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The map label for this gene is odhB [H]

Identifier: 119897847

GI number: 119897847

Start: 1706183

End: 1707385

Strand: Direct

Name: odhB [H]

Synonym: azo1556

Alternate gene names: 119897847

Gene position: 1706183-1707385 (Clockwise)

Preceding gene: 119897846

Following gene: 119897848

Centisome position: 38.99

GC content: 67.41

Gene sequence:

>1203_bases
ATGCTGATCGAAGTGAAAGTGCCGCAGCTGTCCGAGTCCGTTTCCGAAGCCACGCTGGTCACCTGGCACAAGAAGGAAGG
CGACGCGGTCACCCGTGACGAGAACCTGATCGACATCGAGACCGACAAGGTCGTGCTGGAAACCCCGGCCCCGGCCGATG
GCGTGCTGGTCAAGATCATCAAGGGCGACGGCGGCACTGTGACGTCCGGCGAACTGATCGCCCAGATCGACACCGAAGCC
AAGGCCGCCGCCGGCGGCGCCAAGGCCGCGGAGCCCGTGCAGGCGGTGACCCCGCCGCCGGCCGCCCCCGCTGCCGCGCC
GTCCGCCGCTGCTGGCGCCGCCAGCCCGGCTGCGCGCAAGATCCTTGAAGAGAAGGGCATCGCCGCGGGCGACGTGGCCG
GCTCCGGCCGTGGCGGCCGCGTCACCAAGGAAGACGCCGTGGGCGCCAGCGCCAAGCCGGCGGCTGCCGCTGCTGCGGCC
CCGGCCGTGGTGATCGCCGGCGAGCGTCCGGAAGAGCGCGTGCCGATGACCCGCCTGCGCGCCCGCATCGCCGAGCGCCT
GCTGCAGTCGAAGAACGAGAACGCCATCCTCACCACGTTCAACGAAGTGAACATGGCCCCGGTGATGGCGCTGCGCAAGC
AGTACGGCGACAAGTTCGAGAAGGCCCACGGCGTGCGCCTGGGCTTCATGGGCTTCTTCGTCAAGGCCGCCGTGGCCGCG
CTGAAGAAGTTCCCGATCCTGAACGCCTCGGTCGATGGCAACGACATCGTCTACCACGGCTACATCGACATCGGCATCGC
GGTCGGTTCGCCGCGTGGCCTGGTCGTGCCGATCCTGCGTGACGCCGAATCGATGTCGATCGCCGACATCGAGAAGAAGA
TCGCCGAGTTCGGCCAGAAGGCCAAGGACGGCAAGCTGTCGCTGGAAGAACTCTCCGGCGGCACCTTCTCGATCTCCAAC
GGTGGCGTGTTCGGTTCGATGCTGTCGACCCCGATCATCAACCCGCCGCAGTCCGCCATCCTCGGCATCCACGCCACCAA
GGACCGTCCGGTGGTCGAGAACGGCCAGATCGTGATCCGCCCGATCAACTACCTGGCGATGTCCTACGACCACCGCATCA
TCGACGGCCGCGAAGCGGTGCTCGGCCTGGTGACGATGAAGGAAGCGCTGGAAGATCCGGCCCGCCTGATCCTCGACGTC
TAA

Upstream 100 bases:

>100_bases
GCAGCAGAAGGCCGTGATCGAAAACGCCTTCGGTCCGATCCAGGACTGATCCGCGCTCAATGACAACGAAAGACTCCGAA
TAACAAGGGGAGAGTATTTC

Downstream 100 bases:

>100_bases
CACACGTAGTCGGGGCGCAACCCGCGGGGAACCTCGGTTCCCGGCGTGGCTGCGCCCCGTTTTCCTGCAAGGGAGTTCTC
AATGTCCAAAGAATTCGACG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MLIEVKVPQLSESVSEATLVTWHKKEGDAVTRDENLIDIETDKVVLETPAPADGVLVKIIKGDGGTVTSGELIAQIDTEA
KAAAGGAKAAEPVQAVTPPPAAPAAAPSAAAGAASPAARKILEEKGIAAGDVAGSGRGGRVTKEDAVGASAKPAAAAAAA
PAVVIAGERPEERVPMTRLRARIAERLLQSKNENAILTTFNEVNMAPVMALRKQYGDKFEKAHGVRLGFMGFFVKAAVAA
LKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDAESMSIADIEKKIAEFGQKAKDGKLSLEELSGGTFSISN
GGVFGSMLSTPIINPPQSAILGIHATKDRPVVENGQIVIRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLILDV

Sequences:

>Translated_400_residues
MLIEVKVPQLSESVSEATLVTWHKKEGDAVTRDENLIDIETDKVVLETPAPADGVLVKIIKGDGGTVTSGELIAQIDTEA
KAAAGGAKAAEPVQAVTPPPAAPAAAPSAAAGAASPAARKILEEKGIAAGDVAGSGRGGRVTKEDAVGASAKPAAAAAAA
PAVVIAGERPEERVPMTRLRARIAERLLQSKNENAILTTFNEVNMAPVMALRKQYGDKFEKAHGVRLGFMGFFVKAAVAA
LKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDAESMSIADIEKKIAEFGQKAKDGKLSLEELSGGTFSISN
GGVFGSMLSTPIINPPQSAILGIHATKDRPVVENGQIVIRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLILDV
>Mature_400_residues
MLIEVKVPQLSESVSEATLVTWHKKEGDAVTRDENLIDIETDKVVLETPAPADGVLVKIIKGDGGTVTSGELIAQIDTEA
KAAAGGAKAAEPVQAVTPPPAAPAAAPSAAAGAASPAARKILEEKGIAAGDVAGSGRGGRVTKEDAVGASAKPAAAAAAA
PAVVIAGERPEERVPMTRLRARIAERLLQSKNENAILTTFNEVNMAPVMALRKQYGDKFEKAHGVRLGFMGFFVKAAVAA
LKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDAESMSIADIEKKIAEFGQKAKDGKLSLEELSGGTFSISN
GGVFGSMLSTPIINPPQSAILGIHATKDRPVVENGQIVIRPINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLILDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=254, Percent_Identity=50.3937007874016, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI203098753, Length=456, Percent_Identity=30.7017543859649, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI203098816, Length=456, Percent_Identity=30.7017543859649, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI110671329, Length=429, Percent_Identity=27.972027972028, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=29.4663573085847, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI260898739, Length=166, Percent_Identity=37.9518072289157, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786946, Length=403, Percent_Identity=56.0794044665012, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=31.0588235294118, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=409, Percent_Identity=43.520782396088, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=433, Percent_Identity=32.5635103926097, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=26.1904761904762, Blast_Score=158, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=30.7443365695793, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320352, Length=395, Percent_Identity=44.3037974683544, Blast_Score=332, Evalue=5e-92,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=29.646017699115, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=53.6480686695279, Blast_Score=274, Evalue=9e-74,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=27.3781902552204, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24582497, Length=288, Percent_Identity=32.2916666666667, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI20129315, Length=288, Percent_Identity=32.2916666666667, Blast_Score=132, Evalue=5e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 41721; Mature: 41721

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIEVKVPQLSESVSEATLVTWHKKEGDAVTRDENLIDIETDKVVLETPAPADGVLVKII
CEEEEECCHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEECCEEEEECCCCCCCEEEEEE
KGDGGTVTSGELIAQIDTEAKAAAGGAKAAEPVQAVTPPPAAPAAAPSAAAGAASPAARK
ECCCCEEECCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ILEEKGIAAGDVAGSGRGGRVTKEDAVGASAKPAAAAAAAPAVVIAGERPEERVPMTRLR
HHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHCCCEEEEECCCCHHCCCHHHHH
ARIAERLLQSKNENAILTTFNEVNMAPVMALRKQYGDKFEKAHGVRLGFMGFFVKAAVAA
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHCCCEEHHHHHHHHHHHHH
LKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDAESMSIADIEKKIAEFGQK
HHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
AKDGKLSLEELSGGTFSISNGGVFGSMLSTPIINPPQSAILGIHATKDRPVVENGQIVIR
CCCCCCCHHHCCCCEEEECCCCEEHHHHCCCCCCCCHHHEEEEEECCCCCEEECCCEEEE
PINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLILDV
EHHHHHEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC
>Mature Secondary Structure
MLIEVKVPQLSESVSEATLVTWHKKEGDAVTRDENLIDIETDKVVLETPAPADGVLVKII
CEEEEECCHHHHHHHHCEEEEEECCCCCCCCCCCCEEEEECCEEEEECCCCCCCEEEEEE
KGDGGTVTSGELIAQIDTEAKAAAGGAKAAEPVQAVTPPPAAPAAAPSAAAGAASPAARK
ECCCCEEECCCEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
ILEEKGIAAGDVAGSGRGGRVTKEDAVGASAKPAAAAAAAPAVVIAGERPEERVPMTRLR
HHHHCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHCCCEEEEECCCCHHCCCHHHHH
ARIAERLLQSKNENAILTTFNEVNMAPVMALRKQYGDKFEKAHGVRLGFMGFFVKAAVAA
HHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCHHHHHHCCCEEHHHHHHHHHHHHH
LKKFPILNASVDGNDIVYHGYIDIGIAVGSPRGLVVPILRDAESMSIADIEKKIAEFGQK
HHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHCCC
AKDGKLSLEELSGGTFSISNGGVFGSMLSTPIINPPQSAILGIHATKDRPVVENGQIVIR
CCCCCCCHHHCCCCEEEECCCCEEHHHHCCCCCCCCHHHEEEEEECCCCCEEECCCEEEE
PINYLAMSYDHRIIDGREAVLGLVTMKEALEDPARLILDV
EHHHHHEECCCEEECCHHHHHHHHHHHHHHCCCHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]