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The map label for this gene is mdh

Identifier: 119897838

GI number: 119897838

Start: 1696246

End: 1697238

Strand: Reverse

Name: mdh

Synonym: azo1547

Alternate gene names: 119897838

Gene position: 1697238-1696246 (Counterclockwise)

Preceding gene: 119897849

Following gene: 119897837

Centisome position: 38.78

GC content: 64.95

Gene sequence:

>993_bases
ATGAGCAAAGCCCCTGTGCGCGTTGCCGTCACCGGCGCGGCCGGCCAGATCGGTTACAGCCTGCTGTTCCGCATCGCGAG
TGGCGAAATGCTGGGCAAGGACCAGCCCGTCATCCTGCAACTGCTCGACCTGCCCCAGGCCCAGAAGGCGGTCAAGGGCG
TCATGATGGAACTGGAAGACTGCGCCTTCCCGCTGCTGGCCGGCATGATCGCCACCGACGACCCCAACGTCGCCTTCAAG
GACGCCAAGGTTGCACTGCTGGTCGGCGCCCGTCCGCGCGGCCCCGGCATGGAGCGCAAGGACCTGCTGACCGAAAACGC
CAAGATCTTCACCGTTCAGGGCGCTGCGATCGGCCAGTACGCTGATCCGGACTGCAAGGTGCTGGTCGTCGGCAACCCGT
GCAACACCAACGCCTACATCGCCAAGGAAGTCGCGCAGAAGTACGGCCGCGTTCCGGCGAAGAACTTCACCGGCATGCTG
CGCCTGGACCACAACCGCGCGCTGTCGCAACTGGCCGGCAAGTCGGGCCGTGAAGTCTCCAGCCTGAAGAACCTGGTCGT
GTGGGGCAACCACTCGCCCACCATGTACGCCGACTACCGCTTCGTTAAGTCGAACGGCGACAGCGTCAAGGACCTGATCA
ACGACGCGGCCTGGAACAAGGACGTGTTCCTGCCCACCGTCGGCAAGCGCGGCGCCGCCATCATCGAAGCGCGCGGCCTG
TCGTCCGCCGCCTCGGCTGCCAACGCGGCCATCGACCATATCCGCGACTGGGTGCTCGGCTCCAACGGCGAATGGGTCAC
CATGGGCATCCCGTCCGACGGTTCCTACGGCATCCCCGAAGGCGTCATCTACGGCTTCCCGGTCACCACCGAAAACGGTG
AGTACAAGATCGTCCAGGGTCTGGAAATCGACGAGTTCTCGCGCGAGCGCATGACCGTCACCCTGAACGAGCTGCTGGAA
GAGCGCGAAGGCGTGAAGGACCTGCTGGCCTGA

Upstream 100 bases:

>100_bases
GTTGCACGGGATCGAGTCCGCCCTTCCCGCCCAGCACGCGGCAGCGCCGCCGAGACCGCAAAACCTTCATCCAGACCGTC
TTCTTAGAGGGAGTATTCAC

Downstream 100 bases:

>100_bases
TCCACCCCGGCTCCAGCTCTAAGGCGCATGGCTCCGGCCATGCGCCTTTTTCGTTTACCGCCCCGCTGCTATCCTTCGCC
GCCATTCCCTGACGCCGAGA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLPQAQKAVKGVMMELEDCAFPLLAGMIATDDPNVAFK
DAKVALLVGARPRGPGMERKDLLTENAKIFTVQGAAIGQYADPDCKVLVVGNPCNTNAYIAKEVAQKYGRVPAKNFTGML
RLDHNRALSQLAGKSGREVSSLKNLVVWGNHSPTMYADYRFVKSNGDSVKDLINDAAWNKDVFLPTVGKRGAAIIEARGL
SSAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQGLEIDEFSRERMTVTLNELLE
EREGVKDLLA

Sequences:

>Translated_330_residues
MSKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLPQAQKAVKGVMMELEDCAFPLLAGMIATDDPNVAFK
DAKVALLVGARPRGPGMERKDLLTENAKIFTVQGAAIGQYADPDCKVLVVGNPCNTNAYIAKEVAQKYGRVPAKNFTGML
RLDHNRALSQLAGKSGREVSSLKNLVVWGNHSPTMYADYRFVKSNGDSVKDLINDAAWNKDVFLPTVGKRGAAIIEARGL
SSAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQGLEIDEFSRERMTVTLNELLE
EREGVKDLLA
>Mature_329_residues
SKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLPQAQKAVKGVMMELEDCAFPLLAGMIATDDPNVAFKD
AKVALLVGARPRGPGMERKDLLTENAKIFTVQGAAIGQYADPDCKVLVVGNPCNTNAYIAKEVAQKYGRVPAKNFTGMLR
LDHNRALSQLAGKSGREVSSLKNLVVWGNHSPTMYADYRFVKSNGDSVKDLINDAAWNKDVFLPTVGKRGAAIIEARGLS
SAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQGLEIDEFSRERMTVTLNELLEE
REGVKDLLA

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=332, Percent_Identity=51.8072289156626, Blast_Score=331, Evalue=4e-91,
Organism=Homo sapiens, GI89886456, Length=337, Percent_Identity=26.1127596439169, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17561064, Length=325, Percent_Identity=54.1538461538462, Blast_Score=337, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI32566798, Length=262, Percent_Identity=53.8167938931298, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=51.7647058823529, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6322765, Length=168, Percent_Identity=32.7380952380952, Blast_Score=66, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24583394, Length=324, Percent_Identity=52.7777777777778, Blast_Score=321, Evalue=5e-88,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_AZOSB (A1K5Q9)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_933051.1
- ProteinModelPortal:   A1K5Q9
- SMR:   A1K5Q9
- STRING:   A1K5Q9
- GeneID:   4608485
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1547
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- PhylomeDB:   A1K5Q9
- ProtClustDB:   PRK05442
- BioCyc:   ASP62928:AZO1547-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35527; Mature: 35396

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 191-191 BINDING 93-93 BINDING 99-99 BINDING 106-106 BINDING 113-113 BINDING 132-132 BINDING 166-166

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLPQAQKAVKGVMMELED
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHH
CAFPLLAGMIATDDPNVAFKDAKVALLVGARPRGPGMERKDLLTENAKIFTVQGAAIGQY
HHHHHHHCCCCCCCCCEEEECCEEEEEEECCCCCCCCCHHHHHCCCCEEEEEECCEECCC
ADPDCKVLVVGNPCNTNAYIAKEVAQKYGRVPAKNFTGMLRLDHNRALSQLAGKSGREVS
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHCCCCCCHH
SLKNLVVWGNHSPTMYADYRFVKSNGDSVKDLINDAAWNKDVFLPTVGKRGAAIIEARGL
HHCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCC
SSAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQG
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECEEECCCCCEEEEEC
LEIDEFSRERMTVTLNELLEEREGVKDLLA
CCCCHHCCCCEEEEHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SKAPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLDLPQAQKAVKGVMMELED
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHH
CAFPLLAGMIATDDPNVAFKDAKVALLVGARPRGPGMERKDLLTENAKIFTVQGAAIGQY
HHHHHHHCCCCCCCCCEEEECCEEEEEEECCCCCCCCCHHHHHCCCCEEEEEECCEECCC
ADPDCKVLVVGNPCNTNAYIAKEVAQKYGRVPAKNFTGMLRLDHNRALSQLAGKSGREVS
CCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECHHHHHHHHHCCCCCCHH
SLKNLVVWGNHSPTMYADYRFVKSNGDSVKDLINDAAWNKDVFLPTVGKRGAAIIEARGL
HHCCEEEECCCCCCEEEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCCCEEEEECCC
SSAASAANAAIDHIRDWVLGSNGEWVTMGIPSDGSYGIPEGVIYGFPVTTENGEYKIVQG
CHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEECEEECCCCCEEEEEC
LEIDEFSRERMTVTLNELLEEREGVKDLLA
CCCCHHCCCCEEEEHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA