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The map label for this gene is ynfL [H]

Identifier: 119897834

GI number: 119897834

Start: 1690740

End: 1691627

Strand: Direct

Name: ynfL [H]

Synonym: azo1543

Alternate gene names: 119897834

Gene position: 1690740-1691627 (Clockwise)

Preceding gene: 119897832

Following gene: 119897835

Centisome position: 38.64

GC content: 72.41

Gene sequence:

>888_bases
TTGCGCTACTTCGTCGCGGTTGCCGAGACGCTCAATTTCACCCGCGCGGCCGAGCGCCTGCACATCGGCCAACCCCCCTT
GTCGATGCAGATCCGCGACCTCGAGACGGAGGTCGGCGCCCGGCTGTTCGAGCGCAGCCGGCGCAAGGTGGAGCTGACCG
ATGCCGGCGAGCGCTTCCTCGCCCATGCGCGCGCCATCCTGGCCCGGACCGAGGAGGCGGCCGAGGAGGCGCGCCGGGCG
GCGGCGGGCGAGATCGGCGAACTGCGCGTCGGCATCACGTCGTCGCTGCCCTACACCGCGATCCTGCCCGACGCGCTGGC
GGCGTTCCGCCAGCGCCACCCCGCATTGCGGCTGCAGATCCACGAGATGTTCACCGCGGAGCAGTTCGACGCGCTTGCGC
AGGACAGGCTCGACGTCGGCCTGGTGCGCTTCGACGCGACCCTGGCGCCACCCCATATCCGGGTTCGGGAGATCGGCCGC
GACGCCTTGCGCGTGGTGGTCCATGCCGAGCATCCGCTGGCGGCGAAGGCGAGCGTTTCGTTCGCCGAGTTGCGTGACGC
CGACTTCATCAGCTTCCCGGCGGATGCCGGCACCGAACTGCCCGCCATCCTCGACCGGCTGTGCCGGGGCGCGGGTTTCG
AGCCGCGCGTGGTCCAGCTTGCGCGCGAGGCGACCACGCAGATCGGCCTGGTCGCCGCCGGGCTCGGCATCGCGCTGTTG
CCGGCGCCGCTGGAGTGCGTGCGCATGCCGCGGGTGCGCTACCTGCCGGTGAAGGACGCGGGAGCCGACTTCCCGCTCGC
CGTGGCCTGCCATGCGCATCGCACCCGCGCCGTGACCGATCGCTTCCTCGCGGTGCTGGACGAGGTGCGCACCACCCGCG
CGGGCTGA

Upstream 100 bases:

>100_bases
CAAGGCGAGCCATACAGTGATGCTAGGTGTGCGCGAATACGCAGTCCAATATATTCTTTAGTGTCGATTGATACTTTTTA
AATATATGGAACTGCGCCAC

Downstream 100 bases:

>100_bases
CACGCCGACGGGCGGCATTTCGCCCGCCGCGGCGGGCCGCGTAGAATGACGTCTCGATTCATCCCTCCCGCGCCTCTTCT
GCTTATGCCTGTTTCTCCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MRYFVAVAETLNFTRAAERLHIGQPPLSMQIRDLETEVGARLFERSRRKVELTDAGERFLAHARAILARTEEAAEEARRA
AAGEIGELRVGITSSLPYTAILPDALAAFRQRHPALRLQIHEMFTAEQFDALAQDRLDVGLVRFDATLAPPHIRVREIGR
DALRVVVHAEHPLAAKASVSFAELRDADFISFPADAGTELPAILDRLCRGAGFEPRVVQLAREATTQIGLVAAGLGIALL
PAPLECVRMPRVRYLPVKDAGADFPLAVACHAHRTRAVTDRFLAVLDEVRTTRAG

Sequences:

>Translated_295_residues
MRYFVAVAETLNFTRAAERLHIGQPPLSMQIRDLETEVGARLFERSRRKVELTDAGERFLAHARAILARTEEAAEEARRA
AAGEIGELRVGITSSLPYTAILPDALAAFRQRHPALRLQIHEMFTAEQFDALAQDRLDVGLVRFDATLAPPHIRVREIGR
DALRVVVHAEHPLAAKASVSFAELRDADFISFPADAGTELPAILDRLCRGAGFEPRVVQLAREATTQIGLVAAGLGIALL
PAPLECVRMPRVRYLPVKDAGADFPLAVACHAHRTRAVTDRFLAVLDEVRTTRAG
>Mature_295_residues
MRYFVAVAETLNFTRAAERLHIGQPPLSMQIRDLETEVGARLFERSRRKVELTDAGERFLAHARAILARTEEAAEEARRA
AAGEIGELRVGITSSLPYTAILPDALAAFRQRHPALRLQIHEMFTAEQFDALAQDRLDVGLVRFDATLAPPHIRVREIGR
DALRVVVHAEHPLAAKASVSFAELRDADFISFPADAGTELPAILDRLCRGAGFEPRVVQLAREATTQIGLVAAGLGIALL
PAPLECVRMPRVRYLPVKDAGADFPLAVACHAHRTRAVTDRFLAVLDEVRTTRAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=292, Percent_Identity=41.0958904109589, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1788887, Length=289, Percent_Identity=35.9861591695502, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI2367136, Length=259, Percent_Identity=31.6602316602317, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI157672245, Length=244, Percent_Identity=34.8360655737705, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI145693105, Length=255, Percent_Identity=29.0196078431373, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1790399, Length=247, Percent_Identity=31.5789473684211, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1787530, Length=250, Percent_Identity=28, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87081904, Length=175, Percent_Identity=33.7142857142857, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=26.0330578512397, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788297, Length=142, Percent_Identity=33.0985915492958, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787601, Length=249, Percent_Identity=26.9076305220884, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786713, Length=250, Percent_Identity=28.8, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082132, Length=169, Percent_Identity=31.3609467455621, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=245, Percent_Identity=26.530612244898, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790208, Length=279, Percent_Identity=26.5232974910394, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1787949, Length=240, Percent_Identity=25.8333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1789173, Length=158, Percent_Identity=31.6455696202532, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI145693193, Length=120, Percent_Identity=30.8333333333333, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=24.2214532871972, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787589, Length=244, Percent_Identity=29.9180327868852, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790262, Length=226, Percent_Identity=27.4336283185841, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI87082024, Length=128, Percent_Identity=27.34375, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32413; Mature: 32413

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYFVAVAETLNFTRAAERLHIGQPPLSMQIRDLETEVGARLFERSRRKVELTDAGERFL
CCEEEEHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEECHHHHHHH
AHARAILARTEEAAEEARRAAAGEIGELRVGITSSLPYTAILPDALAAFRQRHPALRLQI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHEEHH
HEMFTAEQFDALAQDRLDVGLVRFDATLAPPHIRVREIGRDALRVVVHAEHPLAAKASVS
HHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHCHHHEEEEEECCCCCCHHCCCH
FAELRDADFISFPADAGTELPAILDRLCRGAGFEPRVVQLAREATTQIGLVAAGLGIALL
HHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCC
PAPLECVRMPRVRYLPVKDAGADFPLAVACHAHRTRAVTDRFLAVLDEVRTTRAG
CCCHHHHCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRYFVAVAETLNFTRAAERLHIGQPPLSMQIRDLETEVGARLFERSRRKVELTDAGERFL
CCEEEEHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEECHHHHHHH
AHARAILARTEEAAEEARRAAAGEIGELRVGITSSLPYTAILPDALAAFRQRHPALRLQI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCHHEEHH
HEMFTAEQFDALAQDRLDVGLVRFDATLAPPHIRVREIGRDALRVVVHAEHPLAAKASVS
HHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHCHHHEEEEEECCCCCCHHCCCH
FAELRDADFISFPADAGTELPAILDRLCRGAGFEPRVVQLAREATTQIGLVAAGLGIALL
HHHHCCCCCEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHCC
PAPLECVRMPRVRYLPVKDAGADFPLAVACHAHRTRAVTDRFLAVLDEVRTTRAG
CCCHHHHCCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]