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The map label for this gene is nuoI

Identifier: 119897695

GI number: 119897695

Start: 1532947

End: 1533432

Strand: Direct

Name: nuoI

Synonym: azo1404

Alternate gene names: 119897695

Gene position: 1532947-1533432 (Clockwise)

Preceding gene: 119897694

Following gene: 119897696

Centisome position: 35.03

GC content: 61.32

Gene sequence:

>486_bases
ATGGGTGCCAAGGATTACATCGGTAGTCTGTTCCTGAAGGAACTCGTGAAGGGCATGGCCCTGACGGGTCGCCATTTCTT
CGCGCGCAAGATCACCGTTCAGTTCCCGGAAGAGAAGACGCCGCAGAGTGCGCGCTTTCGCGGACTGCATGCGCTGCGCC
GCTATCCCAACGGCGAGGAGCGCTGCATCGCCTGCAAGCTGTGCGAAGCGATCTGTCCGGCGATGGCAATCACGATCGAG
TCGGAGCAGCGTGAAGACGGTTCGCGCCGGACCAGCCGCTACGATATCGACCTGACCAAGTGCATCTTCTGTGGCTTCTG
CGAGGAAGCCTGCCCGGTGGATGCGGTCGTTGAAACCCGCGTGTTCGAGTATCACGGTGAGCAGCGCGGCGATCTGTACT
ACACGAAGCAGATGCTGCTCGCAGTGGGCGACCGCCACGAGCAGCAGATCGCCGCCGACCGCGAACAAGAGGCGAAATTC
CGCTAA

Upstream 100 bases:

>100_bases
TTGGGCTGGAAGGTGTTCATTCCGGTCACCCTGGTCTGGGTGGTCGTGGTCGCGGTGTGGTTGATGTCGCCGCTGTCGAT
CTGGAAGTAAGAGGCTGAAC

Downstream 100 bases:

>100_bases
AGGGGAGGGCGCCCGCTGGCGCCGTGGATACTGAACATGGAATTCAAGACGGCTGTCTTCTACTTTTTGGCCGCGATCCT
GGTGTTTGCCGGATTGCGCG

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MGAKDYIGSLFLKELVKGMALTGRHFFARKITVQFPEEKTPQSARFRGLHALRRYPNGEERCIACKLCEAICPAMAITIE
SEQREDGSRRTSRYDIDLTKCIFCGFCEEACPVDAVVETRVFEYHGEQRGDLYYTKQMLLAVGDRHEQQIAADREQEAKF
R

Sequences:

>Translated_161_residues
MGAKDYIGSLFLKELVKGMALTGRHFFARKITVQFPEEKTPQSARFRGLHALRRYPNGEERCIACKLCEAICPAMAITIE
SEQREDGSRRTSRYDIDLTKCIFCGFCEEACPVDAVVETRVFEYHGEQRGDLYYTKQMLLAVGDRHEQQIAADREQEAKF
R
>Mature_160_residues
GAKDYIGSLFLKELVKGMALTGRHFFARKITVQFPEEKTPQSARFRGLHALRRYPNGEERCIACKLCEAICPAMAITIES
EQREDGSRRTSRYDIDLTKCIFCGFCEEACPVDAVVETRVFEYHGEQRGDLYYTKQMLLAVGDRHEQQIAADREQEAKFR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=153, Percent_Identity=58.8235294117647, Blast_Score=193, Evalue=6e-50,
Organism=Escherichia coli, GI1788617, Length=128, Percent_Identity=41.40625, Blast_Score=108, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555194, Length=153, Percent_Identity=58.8235294117647, Blast_Score=194, Evalue=2e-50,
Organism=Drosophila melanogaster, GI17864306, Length=154, Percent_Identity=59.0909090909091, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_AZOSB (A1K5B6)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932908.1
- ProteinModelPortal:   A1K5B6
- SMR:   A1K5B6
- STRING:   A1K5B6
- GeneID:   4609426
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1404
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- PhylomeDB:   A1K5B6
- ProtClustDB:   PRK05888
- BioCyc:   ASP62928:AZO1404-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18508; Mature: 18377

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAKDYIGSLFLKELVKGMALTGRHFFARKITVQFPEEKTPQSARFRGLHALRRYPNGEE
CCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCH
RCIACKLCEAICPAMAITIESEQREDGSRRTSRYDIDLTKCIFCGFCEEACPVDAVVETR
HHHHHHHHHHHCCHHEEEECCHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
VFEYHGEQRGDLYYTKQMLLAVGDRHEQQIAADREQEAKFR
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHCCCCC
>Mature Secondary Structure 
GAKDYIGSLFLKELVKGMALTGRHFFARKITVQFPEEKTPQSARFRGLHALRRYPNGEE
CCHHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCH
RCIACKLCEAICPAMAITIESEQREDGSRRTSRYDIDLTKCIFCGFCEEACPVDAVVETR
HHHHHHHHHHHCCHHEEEECCHHCCCCHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
VFEYHGEQRGDLYYTKQMLLAVGDRHEQQIAADREQEAKFR
HHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA