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The map label for this gene is FtsJ

Identifier: 119897677

GI number: 119897677

Start: 1514707

End: 1515324

Strand: Direct

Name: FtsJ

Synonym: azo1386

Alternate gene names: 119897677

Gene position: 1514707-1515324 (Clockwise)

Preceding gene: 119897675

Following gene: 119897678

Centisome position: 34.61

GC content: 62.62

Gene sequence:

>618_bases
ATGAAGCGGAACAAGACCAGCAAGGCGTGGATGCACGAGCATCTGAACGACACCTACGTGCAACGCGCGAATGCCGAGGG
ATACCGTGCGCGCGCCGCGTACAAGTTGATGGAGATCGACGAGCGGGACCGGCTGTTGAGACCAGGGCGTGTCGTTGTCG
ACCTTGGCGCCGCGCCGGGGTCGTGGTGTCAGGTGGCGCGCCAGCGTGTCGGTTCCGATGGGCGAGTGCTTGCGCTGGAC
ATTCTCCCGATGGACCCGGTCCCCGGCGTGGACTTTCTCCAGGGCGATTTCACCGAGGACGCGGTTCTGGCGGAACTCGA
ATCTCGCCTCGCCGGTGCGGCGGTAGACCTTGTGCTTTCAGACATGGCCCCCAACTTGTCCGGTGTGGCAACGGTCGATC
AGGCGCGCTCCATTTACTTGTGCGAACTCGCGCTGGATTTTGCCCGGCGACATCTCAAGCCCGGCGGTCAGTTCCTGGTG
AAGGTGTTTCAGGGGGAAGGCTTCATGGGATTCCGCAAGGCCATGGACGAAGTGTTCCTTTCGGTTCAGGTTCGCAAGCC
GAAGGCGTCGCGGGACCGCAGTGCGGAGGTCTATCTGCTAGGCGTCGATTTGCGCTGA

Upstream 100 bases:

>100_bases
GAGGTGGTGGGCGCTGGCGCGCAGTTCGCGGCGCCGGGCGGGCGTGAGCTGGATCATCGATTTTTTCTCCGTGGGGCGGG
GATTCTACTCTCTTAGGCAA

Downstream 100 bases:

>100_bases
TTTCCGCGTGCTTAATAGGGTTTGACAATCGTTCCCATGGTTTGCGGGTGGGAACGATCCCCTCTAGAATGGGTCCATCG
AACGCACGCCCTATTGGGGC

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKRNKTSKAWMHEHLNDTYVQRANAEGYRARAAYKLMEIDERDRLLRPGRVVVDLGAAPGSWCQVARQRVGSDGRVLALD
ILPMDPVPGVDFLQGDFTEDAVLAELESRLAGAAVDLVLSDMAPNLSGVATVDQARSIYLCELALDFARRHLKPGGQFLV
KVFQGEGFMGFRKAMDEVFLSVQVRKPKASRDRSAEVYLLGVDLR

Sequences:

>Translated_205_residues
MKRNKTSKAWMHEHLNDTYVQRANAEGYRARAAYKLMEIDERDRLLRPGRVVVDLGAAPGSWCQVARQRVGSDGRVLALD
ILPMDPVPGVDFLQGDFTEDAVLAELESRLAGAAVDLVLSDMAPNLSGVATVDQARSIYLCELALDFARRHLKPGGQFLV
KVFQGEGFMGFRKAMDEVFLSVQVRKPKASRDRSAEVYLLGVDLR
>Mature_205_residues
MKRNKTSKAWMHEHLNDTYVQRANAEGYRARAAYKLMEIDERDRLLRPGRVVVDLGAAPGSWCQVARQRVGSDGRVLALD
ILPMDPVPGVDFLQGDFTEDAVLAELESRLAGAAVDLVLSDMAPNLSGVATVDQARSIYLCELALDFARRHLKPGGQFLV
KVFQGEGFMGFRKAMDEVFLSVQVRKPKASRDRSAEVYLLGVDLR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=210, Percent_Identity=40.4761904761905, Blast_Score=151, Evalue=3e-37,
Organism=Homo sapiens, GI29029591, Length=184, Percent_Identity=38.5869565217391, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI29029589, Length=184, Percent_Identity=38.5869565217391, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI7110661, Length=184, Percent_Identity=38.5869565217391, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI194097365, Length=184, Percent_Identity=34.7826086956522, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1789569, Length=199, Percent_Identity=51.7587939698493, Blast_Score=219, Evalue=7e-59,
Organism=Caenorhabditis elegans, GI17553474, Length=214, Percent_Identity=35.0467289719626, Blast_Score=129, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17554650, Length=190, Percent_Identity=35.7894736842105, Blast_Score=128, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71987550, Length=184, Percent_Identity=35.8695652173913, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71987561, Length=184, Percent_Identity=35.8695652173913, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17553860, Length=184, Percent_Identity=35.8695652173913, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319796, Length=185, Percent_Identity=34.5945945945946, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319535, Length=194, Percent_Identity=32.4742268041237, Blast_Score=111, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321302, Length=90, Percent_Identity=43.3333333333333, Blast_Score=88, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21356387, Length=212, Percent_Identity=37.7358490566038, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859957, Length=184, Percent_Identity=33.695652173913, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24647580, Length=198, Percent_Identity=32.8282828282828, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=193, Percent_Identity=30.5699481865285, Blast_Score=95, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_AZOSB (A1K598)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932890.1
- ProteinModelPortal:   A1K598
- SMR:   A1K598
- STRING:   A1K598
- GeneID:   4606842
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1386
- eggNOG:   COG0293
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   A1K598
- ProtClustDB:   CLSK766882
- BioCyc:   ASP62928:AZO1386-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 22793; Mature: 22793

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNKTSKAWMHEHLNDTYVQRANAEGYRARAAYKLMEIDERDRLLRPGRVVVDLGAAPG
CCCCCCHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCC
SWCQVARQRVGSDGRVLALDILPMDPVPGVDFLQGDFTEDAVLAELESRLAGAAVDLVLS
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
DMAPNLSGVATVDQARSIYLCELALDFARRHLKPGGQFLVKVFQGEGFMGFRKAMDEVFL
HCCCCCCCEEEHHHCCCEEHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHH
SVQVRKPKASRDRSAEVYLLGVDLR
EEEEECCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKRNKTSKAWMHEHLNDTYVQRANAEGYRARAAYKLMEIDERDRLLRPGRVVVDLGAAPG
CCCCCCHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCC
SWCQVARQRVGSDGRVLALDILPMDPVPGVDFLQGDFTEDAVLAELESRLAGAAVDLVLS
HHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
DMAPNLSGVATVDQARSIYLCELALDFARRHLKPGGQFLVKVFQGEGFMGFRKAMDEVFL
HCCCCCCCEEEHHHCCCEEHHHHHHHHHHHHCCCCHHEEEEEECCCCCHHHHHHHHHHHH
SVQVRKPKASRDRSAEVYLLGVDLR
EEEEECCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA