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The map label for this gene is nadE [H]

Identifier: 119897651

GI number: 119897651

Start: 1480139

End: 1481755

Strand: Direct

Name: nadE [H]

Synonym: azo1360

Alternate gene names: 119897651

Gene position: 1480139-1481755 (Clockwise)

Preceding gene: 119897649

Following gene: 119897652

Centisome position: 33.82

GC content: 65.37

Gene sequence:

>1617_bases
ATGAATACCGCGCTTTCCATTGCCGTCGCCCAGTTGAACTTCACCGTGGGCGATCTCGTCGGCAACGCCGACCGCATCAT
CGAAGCCATTTCCGCCGCGCGCGAGCGCGGCGCCGGCCTGCTGATTACCCCTGAACTCGCGCTGTCCGGCTATCCCCCCG
AGGATCTGCTGCTACGCCCGGATTTCTATCGCGGCTGCGCGCGTGAGGTGCGCCGCATTGCCGGGCATTGTCGCGACTTC
TGCCTGGTGCTCGGCCATCCCACCGAGCGCGGCGGGGTGTATTACAACGCCGCGTCCGTGATCCGGGATGGCGAGGTGAT
CGCGACCTACCACAAGCACCTGCTGCCCAATTACGAGGTCTTCGACGAGGAGCGCTATTTCGAATCCGGTGTCGCCCCCT
GCGTTTTCGAGCATGGCGGGGTGCGGATCGGCGTGAACATCTGTGCCGACGTGTGGGAGTCAGGTCCCGCGGAGGTCGCG
CGAGCGGCGGGGGCCGAAGTGCTGGTGTCGCTCAACGCGTCTCCCTTCCATATCGACAAGCAGCAATTGCGCTACGCGGT
GCTGCGCGATCGGGTGCGGGAAACCCGGTTGCCGGTGCTTTACTGCAACATGGTCGGCGGCCAGGACGAACTCGTTTTCG
ACGGCGCCTCCTTCGCCCTCGACCGTGACGGTACGGTGGCCTACCAGTCCGAGGCGTTTGCAGCGTGCATCGACGTGCTG
CGTTTCGAGCAGGGGCGCTGGAGCGGCGGGGGGCACGCGGCGCCCAAGGGTACCGAGGCCGATATCTACGCCGCGCTCGT
CTGCGGGGTGCGCGATTACCTCGGCAAGAACGGCTTTCCCGGCGCGATCATCGGCCTGTCCGGCGGAATCGACTCGGCGC
TCACGCTGGCGGTGGCCGTGGATGCGCTGGGTGCGGACCGCGTGCACGCGGTGATGATGCCGTCGCCCTATACGGCCCAG
ATGAGCCTTGATGATTCCCGCGAGATGGTGAAGCGCCTGGGCGTGCGCTACGACGAGATCGCGATCGAGCCGGCGATGAA
TGTGTTCGCTGACCTGCTCGCGCCGCAGTTCGCCGGCCTCGCGGCCGACACTACCGAAGAGAATCTCCAGAGCCGCATCC
GCGGCATGATCCTGATGGCGCTGTCCAACAAGACCGGGGCGATCGTGCTCACCACCGGCAACAAGAGCGAGATGGCCACC
GGTTACGCCACGCTGTACGGCGACATGGCGGGCGGCTTTGCGGTGCTGAAGGACCTCTACAAGACCACGGTCTATCGCCT
CGCGGCCTGGCGCAACAGCGTGGGCGAGGTCATTCCGCAGAACATCATCGACCGGCCGCCTTCGGCCGAACTGAAGCCGG
ACCAGAAGGATCAGGACAGCCTTCCGCCTTACGAAGTGCTCGACGCGATCATCCAGGCCTACATGGAACACGATGAGTCG
CCGCGCGAGATCATCGCCCGCGGTCTGCCGGAGGCCGACGTGCGGCGCACGGTGACCATGCTCAAGCGCAACGAATACAA
GCGGCGCCAGGCGCCGGTGGGTATCCGGGTGACACAGCGCGGCTTCGGCAGGGACTGGCGTTATCCGATAACATCACGCT
ATCAAGACGAATTCTGA

Upstream 100 bases:

>100_bases
TCCGCTTGGGCGGCTCGTCGTGGAAATGCCGCCCGGCCCGCTGGCCGTGGTGCGACCGTGCCCCCTGACCGGGCGCTGCC
GAAACGATATAGTTGAGGTC

Downstream 100 bases:

>100_bases
ACGACGACACTGCGCCCGACGGGGCGGTGCAAGCCCACCGGAGACTCCCATGAAGAAGATCGAAGCCATCATCAAGCCGT
TCAAGCTGGACGAAGTACGT

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolysing] [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MNTALSIAVAQLNFTVGDLVGNADRIIEAISAARERGAGLLITPELALSGYPPEDLLLRPDFYRGCAREVRRIAGHCRDF
CLVLGHPTERGGVYYNAASVIRDGEVIATYHKHLLPNYEVFDEERYFESGVAPCVFEHGGVRIGVNICADVWESGPAEVA
RAAGAEVLVSLNASPFHIDKQQLRYAVLRDRVRETRLPVLYCNMVGGQDELVFDGASFALDRDGTVAYQSEAFAACIDVL
RFEQGRWSGGGHAAPKGTEADIYAALVCGVRDYLGKNGFPGAIIGLSGGIDSALTLAVAVDALGADRVHAVMMPSPYTAQ
MSLDDSREMVKRLGVRYDEIAIEPAMNVFADLLAPQFAGLAADTTEENLQSRIRGMILMALSNKTGAIVLTTGNKSEMAT
GYATLYGDMAGGFAVLKDLYKTTVYRLAAWRNSVGEVIPQNIIDRPPSAELKPDQKDQDSLPPYEVLDAIIQAYMEHDES
PREIIARGLPEADVRRTVTMLKRNEYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF

Sequences:

>Translated_538_residues
MNTALSIAVAQLNFTVGDLVGNADRIIEAISAARERGAGLLITPELALSGYPPEDLLLRPDFYRGCAREVRRIAGHCRDF
CLVLGHPTERGGVYYNAASVIRDGEVIATYHKHLLPNYEVFDEERYFESGVAPCVFEHGGVRIGVNICADVWESGPAEVA
RAAGAEVLVSLNASPFHIDKQQLRYAVLRDRVRETRLPVLYCNMVGGQDELVFDGASFALDRDGTVAYQSEAFAACIDVL
RFEQGRWSGGGHAAPKGTEADIYAALVCGVRDYLGKNGFPGAIIGLSGGIDSALTLAVAVDALGADRVHAVMMPSPYTAQ
MSLDDSREMVKRLGVRYDEIAIEPAMNVFADLLAPQFAGLAADTTEENLQSRIRGMILMALSNKTGAIVLTTGNKSEMAT
GYATLYGDMAGGFAVLKDLYKTTVYRLAAWRNSVGEVIPQNIIDRPPSAELKPDQKDQDSLPPYEVLDAIIQAYMEHDES
PREIIARGLPEADVRRTVTMLKRNEYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF
>Mature_538_residues
MNTALSIAVAQLNFTVGDLVGNADRIIEAISAARERGAGLLITPELALSGYPPEDLLLRPDFYRGCAREVRRIAGHCRDF
CLVLGHPTERGGVYYNAASVIRDGEVIATYHKHLLPNYEVFDEERYFESGVAPCVFEHGGVRIGVNICADVWESGPAEVA
RAAGAEVLVSLNASPFHIDKQQLRYAVLRDRVRETRLPVLYCNMVGGQDELVFDGASFALDRDGTVAYQSEAFAACIDVL
RFEQGRWSGGGHAAPKGTEADIYAALVCGVRDYLGKNGFPGAIIGLSGGIDSALTLAVAVDALGADRVHAVMMPSPYTAQ
MSLDDSREMVKRLGVRYDEIAIEPAMNVFADLLAPQFAGLAADTTEENLQSRIRGMILMALSNKTGAIVLTTGNKSEMAT
GYATLYGDMAGGFAVLKDLYKTTVYRLAAWRNSVGEVIPQNIIDRPPSAELKPDQKDQDSLPPYEVLDAIIQAYMEHDES
PREIIARGLPEADVRRTVTMLKRNEYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321865, Length=699, Percent_Identity=20.4577968526466, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 58993; Mature: 58993

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTALSIAVAQLNFTVGDLVGNADRIIEAISAARERGAGLLITPELALSGYPPEDLLLRP
CCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCHHCEECC
DFYRGCAREVRRIAGHCRDFCLVLGHPTERGGVYYNAASVIRDGEVIATYHKHLLPNYEV
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEHHHHHCCCCCHHHHHHHHCCCCHH
FDEERYFESGVAPCVFEHGGVRIGVNICADVWESGPAEVARAAGAEVLVSLNASPFHIDK
CCHHHHHHCCCCCEEEECCCEEEEHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCCCH
QQLRYAVLRDRVRETRLPVLYCNMVGGQDELVFDGASFALDRDGTVAYQSEAFAACIDVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCEEECCCCCEEECHHHHHHHHHHH
RFEQGRWSGGGHAAPKGTEADIYAALVCGVRDYLGKNGFPGAIIGLSGGIDSALTLAVAV
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
DALGADRVHAVMMPSPYTAQMSLDDSREMVKRLGVRYDEIAIEPAMNVFADLLAPQFAGL
HHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
AADTTEENLQSRIRGMILMALSNKTGAIVLTTGNKSEMATGYATLYGDMAGGFAVLKDLY
CCCCHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHH
KTTVYRLAAWRNSVGEVIPQNIIDRPPSAELKPDQKDQDSLPPYEVLDAIIQAYMEHDES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PREIIARGLPEADVRRTVTMLKRNEYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTALSIAVAQLNFTVGDLVGNADRIIEAISAARERGAGLLITPELALSGYPPEDLLLRP
CCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCHHCCCCCCHHCEECC
DFYRGCAREVRRIAGHCRDFCLVLGHPTERGGVYYNAASVIRDGEVIATYHKHLLPNYEV
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEHHHHHCCCCCHHHHHHHHCCCCHH
FDEERYFESGVAPCVFEHGGVRIGVNICADVWESGPAEVARAAGAEVLVSLNASPFHIDK
CCHHHHHHCCCCCEEEECCCEEEEHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCCCH
QQLRYAVLRDRVRETRLPVLYCNMVGGQDELVFDGASFALDRDGTVAYQSEAFAACIDVL
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEECCCCEEECCCCCEEECHHHHHHHHHHH
RFEQGRWSGGGHAAPKGTEADIYAALVCGVRDYLGKNGFPGAIIGLSGGIDSALTLAVAV
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHH
DALGADRVHAVMMPSPYTAQMSLDDSREMVKRLGVRYDEIAIEPAMNVFADLLAPQFAGL
HHCCCCCEEEEECCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
AADTTEENLQSRIRGMILMALSNKTGAIVLTTGNKSEMATGYATLYGDMAGGFAVLKDLY
CCCCHHHHHHHHHHHEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHHHHH
KTTVYRLAAWRNSVGEVIPQNIIDRPPSAELKPDQKDQDSLPPYEVLDAIIQAYMEHDES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PREIIARGLPEADVRRTVTMLKRNEYKRRQAPVGIRVTQRGFGRDWRYPITSRYQDEF
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]