BacMap

Definition	Azoarcus sp. BH72 chromosome, complete genome.
Accession	NC_008702
Length	4,376,040

Click here to switch to the map view.

The map label for this gene is fabG2 [H]

Identifier: 119897629

GI number: 119897629

Start: 1456606

End: 1457373

Strand: Direct

Name: fabG2 [H]

Synonym: azo1338

Alternate gene names: 119897629

Gene position: 1456606-1457373 (Clockwise)

Preceding gene: 119897628

Following gene: 119897630

Centisome position: 33.29

GC content: 67.71

Gene sequence:

>768_bases
ATGAAAATCGAGAACAGCGTGTTCGTGGTGACTGGCGGCGGATCCGGCCTGGGGGCTGCAACCGCACGGATGCTGGTCGC
CGGCGGCGGCCGGGTCGTGCTGGCCGACGTGAATGCCGGCGCTGGCGAGGCCGTCGCCGCGGAGCTGGGCGCCCATGCCC
GTTTCGTCTCGACTGACGTGACCGACGAGGCCAGCGCGAAGGCGGCTTTCGATTGCGCACTGACGCAGTTCGGCGGCCTC
AACGGGTTGGTCAACTGTGCGGGCGTGGCACCGGCGGAAAAGGTCGTCGGCCGCGAAGCGCCACACCGGCTCGAAGCGTT
TGCACGCACGGTGAACATCAACCTCGTCGGCAGTTTCAACATGATGCGGCTCGCCGCCGACATCATGAGCAAGGCGGCGC
CGAACGAGGAGGGCGAGCGCGGCGTGATCGTCAGCACGGCTTCGGTCGCGGCATACGACGGGCAGGTCGGGCAGGCCGCC
TACGCCGCCTCCAAGGCGGGCATCGTCGGGCTGACCTTGCCGGTGGCGCGCGAACTCGCGCGTTTCGGCATCCGCGTGAT
GACGATTGCGCCGGGCATCATGGAAACGCCGATGCTGACCGGGATGCCGCAGGAAGTGCAGGACTCGCTCGGCAAGATGG
TTCCGTTCCCGTCCCGGCTGGGGCGTCCCGCCGAATACGCTGCGCTCGTGCGCAGCATCATCGAGAACCCCTATCTGAAT
GGTGAAGTGATCCGCCTCGACGGCGCGATCCGCATGGCCGCGAAGTAG

Upstream 100 bases:

>100_bases
GCCGTAATCGGGTGCTGGATCACCGCCGTCCGGTCTATTGACGTTTACGTCAACGTAAACTATTTTCCCCGCATCCATGA
CTGATGCGGAGGAGAGACGG

Downstream 100 bases:

>100_bases
GGCGTGCCTTGTCGTCGGGGCGCGCGTTCAGCCGTCCCGACGCGCGCTTGACCCAGCGCATTGGTCGGTCTGGCGAAATG
TGCTACAACAGCTGCTTCCC

Product: short-chain dehydrogenase family protein

Products: 3-oxoacyl-CoA; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKIENSVFVVTGGGSGLGAATARMLVAGGGRVVLADVNAGAGEAVAAELGAHARFVSTDVTDEASAKAAFDCALTQFGGL
NGLVNCAGVAPAEKVVGREAPHRLEAFARTVNINLVGSFNMMRLAADIMSKAAPNEEGERGVIVSTASVAAYDGQVGQAA
YAASKAGIVGLTLPVARELARFGIRVMTIAPGIMETPMLTGMPQEVQDSLGKMVPFPSRLGRPAEYAALVRSIIENPYLN
GEVIRLDGAIRMAAK

Sequences:

>Translated_255_residues
MKIENSVFVVTGGGSGLGAATARMLVAGGGRVVLADVNAGAGEAVAAELGAHARFVSTDVTDEASAKAAFDCALTQFGGL
NGLVNCAGVAPAEKVVGREAPHRLEAFARTVNINLVGSFNMMRLAADIMSKAAPNEEGERGVIVSTASVAAYDGQVGQAA
YAASKAGIVGLTLPVARELARFGIRVMTIAPGIMETPMLTGMPQEVQDSLGKMVPFPSRLGRPAEYAALVRSIIENPYLN
GEVIRLDGAIRMAAK
>Mature_255_residues
MKIENSVFVVTGGGSGLGAATARMLVAGGGRVVLADVNAGAGEAVAAELGAHARFVSTDVTDEASAKAAFDCALTQFGGL
NGLVNCAGVAPAEKVVGREAPHRLEAFARTVNINLVGSFNMMRLAADIMSKAAPNEEGERGVIVSTASVAAYDGQVGQAA
YAASKAGIVGLTLPVARELARFGIRVMTIAPGIMETPMLTGMPQEVQDSLGKMVPFPSRLGRPAEYAALVRSIIENPYLN
GEVIRLDGAIRMAAK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4758504, Length=252, Percent_Identity=55.5555555555556, Blast_Score=269, Evalue=2e-72,
Organism=Homo sapiens, GI83715985, Length=252, Percent_Identity=51.984126984127, Blast_Score=244, Evalue=8e-65,
Organism=Homo sapiens, GI15277342, Length=270, Percent_Identity=31.1111111111111, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI40254992, Length=248, Percent_Identity=31.4516129032258, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542939, Length=262, Percent_Identity=29.0076335877863, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI59889578, Length=219, Percent_Identity=27.3972602739726, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI224922801, Length=177, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI5031737, Length=254, Percent_Identity=30.3149606299213, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=31.4720812182741, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI32483357, Length=255, Percent_Identity=28.6274509803922, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI22507405, Length=228, Percent_Identity=28.5087719298246, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI40548397, Length=202, Percent_Identity=28.2178217821782, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI214010158, Length=202, Percent_Identity=28.2178217821782, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI214010156, Length=202, Percent_Identity=28.2178217821782, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI22758144, Length=202, Percent_Identity=28.2178217821782, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI150247226, Length=202, Percent_Identity=28.7128712871287, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=28.5714285714286, Blast_Score=65, Evalue=7e-11,
Organism=Homo sapiens, GI4504505, Length=221, Percent_Identity=28.5067873303167, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787335, Length=254, Percent_Identity=30.7086614173228, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI87082100, Length=260, Percent_Identity=29.2307692307692, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789057, Length=266, Percent_Identity=28.9473684210526, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082160, Length=248, Percent_Identity=27.8225806451613, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789378, Length=251, Percent_Identity=27.8884462151394, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787905, Length=254, Percent_Identity=28.740157480315, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1789208, Length=256, Percent_Identity=28.125, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786812, Length=189, Percent_Identity=32.8042328042328, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI2367175, Length=252, Percent_Identity=31.3492063492063, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788459, Length=254, Percent_Identity=25.1968503937008, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=250, Percent_Identity=47.6, Blast_Score=232, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17555706, Length=254, Percent_Identity=34.6456692913386, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25147288, Length=249, Percent_Identity=31.3253012048193, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17561402, Length=265, Percent_Identity=29.0566037735849, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=260, Percent_Identity=28.8461538461538, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=213, Percent_Identity=27.6995305164319, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17559104, Length=261, Percent_Identity=26.8199233716475, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=268, Percent_Identity=25.7462686567164, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562906, Length=265, Percent_Identity=25.6603773584906, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17563726, Length=260, Percent_Identity=25.7692307692308, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193203117, Length=207, Percent_Identity=27.536231884058, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI72000259, Length=266, Percent_Identity=26.3157894736842, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=199, Percent_Identity=28.1407035175879, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=259, Percent_Identity=24.3243243243243, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=232, Percent_Identity=29.7413793103448, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322227, Length=222, Percent_Identity=25.2252252252252, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17737361, Length=252, Percent_Identity=55.952380952381, Blast_Score=282, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24639444, Length=249, Percent_Identity=32.9317269076305, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21357041, Length=229, Percent_Identity=31.0043668122271, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21355319, Length=246, Percent_Identity=27.6422764227642, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24644339, Length=231, Percent_Identity=30.7359307359307, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28571526, Length=229, Percent_Identity=29.2576419213974, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24665243, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221331218, Length=203, Percent_Identity=29.064039408867, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI23397609, Length=200, Percent_Identity=32, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24665247, Length=204, Percent_Identity=28.921568627451, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI221331216, Length=204, Percent_Identity=28.921568627451, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI28571387, Length=222, Percent_Identity=28.8288288288288, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.35

Molecular weight: Translated: 26186; Mature: 26186

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIENSVFVVTGGGSGLGAATARMLVAGGGRVVLADVNAGAGEAVAAELGAHARFVSTDV
CCCCCCEEEEECCCCCCHHHHHHHEEECCCEEEEEECCCCCCHHHHHHHCCCEEEEECCC
TDEASAKAAFDCALTQFGGLNGLVNCAGVAPAEKVVGREAPHRLEAFARTVNINLVGSFN
CCCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHCCCCCHHHHHHHHEEEEEEEECCH
MMRLAADIMSKAAPNEEGERGVIVSTASVAAYDGQVGQAAYAASKAGIVGLTLPVARELA
HHHHHHHHHHHCCCCCCCCCEEEEEECEEEEECCCCCHHHHHHCCCCEEEEHHHHHHHHH
RFGIRVMTIAPGIMETPMLTGMPQEVQDSLGKMVPFPSRLGRPAEYAALVRSIIENPYLN
HHCEEEEEECCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCC
GEVIRLDGAIRMAAK
CEEEEECCEEEECCC
>Mature Secondary Structure
MKIENSVFVVTGGGSGLGAATARMLVAGGGRVVLADVNAGAGEAVAAELGAHARFVSTDV
CCCCCCEEEEECCCCCCHHHHHHHEEECCCEEEEEECCCCCCHHHHHHHCCCEEEEECCC
TDEASAKAAFDCALTQFGGLNGLVNCAGVAPAEKVVGREAPHRLEAFARTVNINLVGSFN
CCCCCHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHCCCCCHHHHHHHHEEEEEEEECCH
MMRLAADIMSKAAPNEEGERGVIVSTASVAAYDGQVGQAAYAASKAGIVGLTLPVARELA
HHHHHHHHHHHCCCCCCCCCEEEEEECEEEEECCCCCHHHHHHCCCCEEEEHHHHHHHHH
RFGIRVMTIAPGIMETPMLTGMPQEVQDSLGKMVPFPSRLGRPAEYAALVRSIIENPYLN
HHCEEEEEECCCHHCCCCCCCCCHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHCCCCC
GEVIRLDGAIRMAAK
CEEEEECCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-3-hydroxyacyl-CoA; NAD+

Specific reaction: (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]