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The map label for this gene is trmA

Identifier: 119897603

GI number: 119897603

Start: 1431039

End: 1432133

Strand: Direct

Name: trmA

Synonym: azo1312

Alternate gene names: 119897603

Gene position: 1431039-1432133 (Clockwise)

Preceding gene: 119897600

Following gene: 119897604

Centisome position: 32.7

GC content: 66.58

Gene sequence:

>1095_bases
ATGTCGCTTCCTCGCTTCGATCCCGCCGAGTACGAAACCCAGCTCGCCGCGAAAATTGCCCGCTTCAAGTCGGATTTCGC
GCCGCTCGACCTGCCCGAACCGGAGGTTTTCCGCTCCGAACCGCTGCACTACCGGCTGCGGGCCGAATTCCGCATCTGGC
ACAGCGAGGGAAGGTTGGACTACGCGATGTTCGACCCCGCCGATCCCAAGCGGCCGGTGCTGATCGACGGTTTTCCGGCG
GCGGCCGCTCCGATTGCCGCAGCCATGCCCGTCCTGCGCGAGCGCGTGATGGCGAGTGAGCCGCTGCGGCGCAAGCTTTT
CCAGGTGGAGTTCCTCGCGACCCTGTCCGGCGAGCTGATGATCAGCCTCGTCTATCACCGCCCGCTGGAAGCGGACTGGG
AAGCGGCGGCGCGCGAACTTGCTGCGGCGATGGGCGTGCAGCTCATCGGGCGCAGCCGCAAGCAGAAGATCGTGCTGGAG
CGCGACTGGGTGCTGGAGTCGTTCGAACTCGACGGGCGCACGCTGCACTACCAGCAGATCGAGGGCAGCTTTTCGCAGCC
CAACGGCGGCGTGAACCGCCAGATGCTGGTGTGGGCGCGCCGCCAGGCCGAGGGTTCTGGCGCGGACCTGCTCGAACTCT
ATTGCGGCAACGGCAACTTCACCGTGGCGCTGGCACCGCTGTTCGGCAAGGTGCTGGCGACCGAGATGAGCAAGTCGTCG
GTCCGTGCCGCGCACTACAACCTCGCCGCCAACGCGGTCGACAACGTCACCATGGTGCGCATGGCGAGCGAGGAAATCAG
CGATGCGCTGGCCGGCGGCCGCGCCTACCGGCGCATGCAGGGCATCGACCTTGCCGGCTACCGTTTCGGCACCCTGTTCG
TCGATCCGCCACGCAGCGGGCTGGATGAAGCCACCGTCGCGCTGGCGCGCCGGTTCGACCGCATCCTCTACATCTCCTGC
AACCCGCAGACGCTGCACGACAACATCGCCGCGCTGCGCGATACGCACGGCATTGCTGCCGCTGCGGCGTTCGACCAGTT
TCCCTATACCCATCACCTCGAGTGCGGCGTATTGCTGCAGAAGACTGCGGCCTGA

Upstream 100 bases:

>100_bases
GCGCGGAGTCGAAGCCGGCCCTCAGTAGGGCCGCAGCCCCGCCTGCGGTATAGTCCCGCCTCCGTCCGCCGGACGGCCCC
TGCTGCCGCTTCACGTTGCC

Downstream 100 bases:

>100_bases
GCAGCGCCGTCCCGACCACCGAACCGAGAATCCAATGAGCAGCCTGCCGAAGTGTCCGAAATGCAGTTCCGAATACACCT
ACGAGGATGGCGAAAACTAC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSLPRFDPAEYETQLAAKIARFKSDFAPLDLPEPEVFRSEPLHYRLRAEFRIWHSEGRLDYAMFDPADPKRPVLIDGFPA
AAAPIAAAMPVLRERVMASEPLRRKLFQVEFLATLSGELMISLVYHRPLEADWEAAARELAAAMGVQLIGRSRKQKIVLE
RDWVLESFELDGRTLHYQQIEGSFSQPNGGVNRQMLVWARRQAEGSGADLLELYCGNGNFTVALAPLFGKVLATEMSKSS
VRAAHYNLAANAVDNVTMVRMASEEISDALAGGRAYRRMQGIDLAGYRFGTLFVDPPRSGLDEATVALARRFDRILYISC
NPQTLHDNIAALRDTHGIAAAAAFDQFPYTHHLECGVLLQKTAA

Sequences:

>Translated_364_residues
MSLPRFDPAEYETQLAAKIARFKSDFAPLDLPEPEVFRSEPLHYRLRAEFRIWHSEGRLDYAMFDPADPKRPVLIDGFPA
AAAPIAAAMPVLRERVMASEPLRRKLFQVEFLATLSGELMISLVYHRPLEADWEAAARELAAAMGVQLIGRSRKQKIVLE
RDWVLESFELDGRTLHYQQIEGSFSQPNGGVNRQMLVWARRQAEGSGADLLELYCGNGNFTVALAPLFGKVLATEMSKSS
VRAAHYNLAANAVDNVTMVRMASEEISDALAGGRAYRRMQGIDLAGYRFGTLFVDPPRSGLDEATVALARRFDRILYISC
NPQTLHDNIAALRDTHGIAAAAAFDQFPYTHHLECGVLLQKTAA
>Mature_363_residues
SLPRFDPAEYETQLAAKIARFKSDFAPLDLPEPEVFRSEPLHYRLRAEFRIWHSEGRLDYAMFDPADPKRPVLIDGFPAA
AAPIAAAMPVLRERVMASEPLRRKLFQVEFLATLSGELMISLVYHRPLEADWEAAARELAAAMGVQLIGRSRKQKIVLER
DWVLESFELDGRTLHYQQIEGSFSQPNGGVNRQMLVWARRQAEGSGADLLELYCGNGNFTVALAPLFGKVLATEMSKSSV
RAAHYNLAANAVDNVTMVRMASEEISDALAGGRAYRRMQGIDLAGYRFGTLFVDPPRSGLDEATVALARRFDRILYISCN
PQTLHDNIAALRDTHGIAAAAAFDQFPYTHHLECGVLLQKTAA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=176, Percent_Identity=28.9772727272727, Blast_Score=66, Evalue=5e-11,
Organism=Homo sapiens, GI51173878, Length=176, Percent_Identity=28.9772727272727, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790403, Length=362, Percent_Identity=50, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1789148, Length=339, Percent_Identity=29.4985250737463, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24666579, Length=166, Percent_Identity=28.3132530120482, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_AZOSB (A1K524)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932816.1
- ProteinModelPortal:   A1K524
- SMR:   A1K524
- STRING:   A1K524
- GeneID:   4606809
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1312
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- PhylomeDB:   A1K524
- ProtClustDB:   PRK05031
- BioCyc:   ASP62928:AZO1312-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 40536; Mature: 40405

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 320-320 BINDING 186-186 BINDING 214-214 BINDING 219-219 BINDING 235-235 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPRFDPAEYETQLAAKIARFKSDFAPLDLPEPEVFRSEPLHYRLRAEFRIWHSEGRLD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEEEEEEEECCCCEE
YAMFDPADPKRPVLIDGFPAAAAPIAAAMPVLRERVMASEPLRRKLFQVEFLATLSGELM
EEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHE
ISLVYHRPLEADWEAAARELAAAMGVQLIGRSRKQKIVLERDWVLESFELDGRTLHYQQI
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCEEEEEEC
EGSFSQPNGGVNRQMLVWARRQAEGSGADLLELYCGNGNFTVALAPLFGKVLATEMSKSS
CCCCCCCCCCCCCEEEEEEHHCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
VRAAHYNLAANAVDNVTMVRMASEEISDALAGGRAYRRMQGIDLAGYRFGTLFVDPPRSG
HHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCHHHHHHCCCEECCEEEEEEEECCCCCC
LDEATVALARRFDRILYISCNPQTLHDNIAALRDTHGIAAAAAFDQFPYTHHLECGVLLQ
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEE
KTAA
ECCC
>Mature Secondary Structure 
SLPRFDPAEYETQLAAKIARFKSDFAPLDLPEPEVFRSEPLHYRLRAEFRIWHSEGRLD
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEEEEEEEEEECCCCEE
YAMFDPADPKRPVLIDGFPAAAAPIAAAMPVLRERVMASEPLRRKLFQVEFLATLSGELM
EEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHE
ISLVYHRPLEADWEAAARELAAAMGVQLIGRSRKQKIVLERDWVLESFELDGRTLHYQQI
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCEEEEEEC
EGSFSQPNGGVNRQMLVWARRQAEGSGADLLELYCGNGNFTVALAPLFGKVLATEMSKSS
CCCCCCCCCCCCCEEEEEEHHCCCCCCCEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHH
VRAAHYNLAANAVDNVTMVRMASEEISDALAGGRAYRRMQGIDLAGYRFGTLFVDPPRSG
HHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHCCHHHHHHCCCEECCEEEEEEEECCCCCC
LDEATVALARRFDRILYISCNPQTLHDNIAALRDTHGIAAAAAFDQFPYTHHLECGVLLQ
HHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEEE
KTAA
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA