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The map label for this gene is dut

Identifier: 119897430

GI number: 119897430

Start: 1226436

End: 1226885

Strand: Direct

Name: dut

Synonym: azo1139

Alternate gene names: 119897430

Gene position: 1226436-1226885 (Clockwise)

Preceding gene: 119897429

Following gene: 119897431

Centisome position: 28.03

GC content: 68.22

Gene sequence:

>450_bases
ATGCACCGCATCGACGTCAAGCTGCTCGATCCCCGTCTGAAAACCCATCCGCCCGCTTATGCCAGTGCCGGCGCTGCGGG
GCTGGACCTGCGCGCCTGTCTCGACGCGCCGGTGCTGCTGCACCCGGGGGAGACGACCCTGGTGCCGAGCGGACTCGCCA
TCCACCTCGCCGACCCCGGGCTCGCCGCCATGGTGCTGCCGCGTTCCGGGCTGGGCCACAAGCACGGGATCGTGCTCGGC
AATCTCGTGGGACTGATCGATTCGGATTATCAGGGGCAGGTTTTCGTGTCGGTGTGGAACCGGGGCCGCGACGTGTTCAC
GATCCAGCCGATGGAGCGCATCGCGCAACTGGTGGTGGTGCCCGTGCTGCAGGTCGGGTTCAACGTCGTGGACGACTTCG
CCGCCAGCGAGCGGGGCGAGGGAGGGTTCGGCAGCACCGGCAAGCACTGA

Upstream 100 bases:

>100_bases
TTGCCACGTGCGCCCAAAACCGAACTCGCCCGACAGATCGTCGACCATCTGGCCGCCCTGCTGCCGCCTTCGAATTCCCG
CTGACCCATCGGAAACCGCC

Downstream 100 bases:

>100_bases
CGTGCAGGCGAAGGGGATTCCCACCGGCGTGCACATCGTGTGCGAGCGTGGTGGACGCGTGTTGTTGATGCGCCGCGCCG
GCACCGGGTTTTTCGACGGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MHRIDVKLLDPRLKTHPPAYASAGAAGLDLRACLDAPVLLHPGETTLVPSGLAIHLADPGLAAMVLPRSGLGHKHGIVLG
NLVGLIDSDYQGQVFVSVWNRGRDVFTIQPMERIAQLVVVPVLQVGFNVVDDFAASERGEGGFGSTGKH

Sequences:

>Translated_149_residues
MHRIDVKLLDPRLKTHPPAYASAGAAGLDLRACLDAPVLLHPGETTLVPSGLAIHLADPGLAAMVLPRSGLGHKHGIVLG
NLVGLIDSDYQGQVFVSVWNRGRDVFTIQPMERIAQLVVVPVLQVGFNVVDDFAASERGEGGFGSTGKH
>Mature_149_residues
MHRIDVKLLDPRLKTHPPAYASAGAAGLDLRACLDAPVLLHPGETTLVPSGLAIHLADPGLAAMVLPRSGLGHKHGIVLG
NLVGLIDSDYQGQVFVSVWNRGRDVFTIQPMERIAQLVVVPVLQVGFNVVDDFAASERGEGGFGSTGKH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI4503423, Length=146, Percent_Identity=39.041095890411, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI70906444, Length=146, Percent_Identity=39.041095890411, Blast_Score=85, Evalue=2e-17,
Organism=Homo sapiens, GI70906441, Length=146, Percent_Identity=39.041095890411, Blast_Score=83, Evalue=7e-17,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=71.523178807947, Blast_Score=223, Evalue=3e-60,
Organism=Caenorhabditis elegans, GI71988561, Length=133, Percent_Identity=42.8571428571429, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=147, Percent_Identity=34.6938775510204, Blast_Score=69, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=36.1538461538462, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583610, Length=130, Percent_Identity=36.1538461538462, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_AZOSB (A1K4K1)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932643.1
- ProteinModelPortal:   A1K4K1
- SMR:   A1K4K1
- STRING:   A1K4K1
- GeneID:   4608619
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1139
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   A1K4K1
- ProtClustDB:   PRK00601
- BioCyc:   ASP62928:AZO1139-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15758; Mature: 15758

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRIDVKLLDPRLKTHPPAYASAGAAGLDLRACLDAPVLLHPGETTLVPSGLAIHLADPG
CCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCEEECCCCEEEEECCC
LAAMVLPRSGLGHKHGIVLGNLVGLIDSDYQGQVFVSVWNRGRDVFTIQPMERIAQLVVV
EEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHH
PVLQVGFNVVDDFAASERGEGGFGSTGKH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MHRIDVKLLDPRLKTHPPAYASAGAAGLDLRACLDAPVLLHPGETTLVPSGLAIHLADPG
CCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCEEECCCCEEEEECCC
LAAMVLPRSGLGHKHGIVLGNLVGLIDSDYQGQVFVSVWNRGRDVFTIQPMERIAQLVVV
EEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHH
PVLQVGFNVVDDFAASERGEGGFGSTGKH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA