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The map label for this gene is livM2 [H]

Identifier: 119897417

GI number: 119897417

Start: 1215207

End: 1216199

Strand: Direct

Name: livM2 [H]

Synonym: azo1126

Alternate gene names: 119897417

Gene position: 1215207-1216199 (Clockwise)

Preceding gene: 119897416

Following gene: 119897418

Centisome position: 27.77

GC content: 68.78

Gene sequence:

>993_bases
ATGAGCAGCCATAACAACGCCTGGCTCGCGCCCGGCCTGCTGCTGGCGGCGCTGGCGGCCTTTCCCGCGCTGGCGCCTCA
CTTCGGCCTCGATTACTACACCGGTTTCGTGATGCGCGTGCTGATCGTGATGCTGATCGCGACCAGCCTCAACTTCCTGA
TGGGCTATGGCGGCATGGTGGCGCTTGGCCACGCCGGCTTCGTCGGTGTCGGCGCCTATGCGCTGGTGGCGATGACCGAG
GCGGGCATCACCAACGCCTGGGCGCTGTGGGGCGGGGCGATGCTGGCGGCGTCGGTGGCCGCGCTGCTGGTGGGCGCGGT
TTCACTGCGCACCCGCGGGGTGTACTTCATCATGATCACGCTCGCCTTCGCGCAGATGCTGTACTACCTGGCGGTGTCGC
TGCGCGCCTATGGCGGCGACGACGGCTACAACCTGATGGCGCGCCCGCTGCTCGGCTTCGGGCTGAACGCCGACGATGAC
GCCACGCTGTACTGGGTGGTGTTGGCGGTGTGCGCGCTGTGCTTCCTGTTCTTCCACCACGCCATCGGCTCGCGTTTCGG
CCAGGCGCTGATCGGCGTGCGCGACAACGAGTCGCGCATGCTGGCGCTGGGCTACCCGGTGTTCCTGCTCAAGCTGCAGG
CCTTCGTGATCGCCGCGGCGGTGGCGGGGCTGGGCGGCGCGCTGATGGTGACGCAGAACAGCTTCATCAGTCCGTCCTCG
ATGCACTGGTCGCAGTCGGCGGTGCTGATCGTCATCGTGGTGCTCGGCGGGTTGGGGCATCGCTGGGGCGGGGTGATCGG
CGCGGCGGTGTGGGTGTCGCTGGAAGAGGTGCTGCGTATGAGCACCGAGTACTGGCACTGGCCGCTCGGCGCGCTGCTGA
TCGCGATCATCCTGTTTGCGCCGGACGGCCTCGGCGCGCTGTGCCGCCGCCGCGCTGCAGGCACCGGCGCCGGCCGCGTC
GGCTTCTTCCATCTGCTGCGGAGGTCGGCATGA

Upstream 100 bases:

>100_bases
CCGACGTCGGCCCGGCGCTGGCCGGCATCGCGATGTACGTGCTGATGGCGGCGATCCTGTGCTTCAAGCCGCGCGGCCTG
TTCCCGGCGAGGGGGTGAAG

Downstream 100 bases:

>100_bases
CGCTTTTCTCCACCCGCGGTCTCGTCAAGCAGTTTGGCCGGCTGCGCGCCACCGACGAGGTTTCGATCGAGGTTGCGCCC
AACGAGGTGCATGCGCTGAT

Product: putative branched-chain amino acid transport permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSSHNNAWLAPGLLLAALAAFPALAPHFGLDYYTGFVMRVLIVMLIATSLNFLMGYGGMVALGHAGFVGVGAYALVAMTE
AGITNAWALWGGAMLAASVAALLVGAVSLRTRGVYFIMITLAFAQMLYYLAVSLRAYGGDDGYNLMARPLLGFGLNADDD
ATLYWVVLAVCALCFLFFHHAIGSRFGQALIGVRDNESRMLALGYPVFLLKLQAFVIAAAVAGLGGALMVTQNSFISPSS
MHWSQSAVLIVIVVLGGLGHRWGGVIGAAVWVSLEEVLRMSTEYWHWPLGALLIAIILFAPDGLGALCRRRAAGTGAGRV
GFFHLLRRSA

Sequences:

>Translated_330_residues
MSSHNNAWLAPGLLLAALAAFPALAPHFGLDYYTGFVMRVLIVMLIATSLNFLMGYGGMVALGHAGFVGVGAYALVAMTE
AGITNAWALWGGAMLAASVAALLVGAVSLRTRGVYFIMITLAFAQMLYYLAVSLRAYGGDDGYNLMARPLLGFGLNADDD
ATLYWVVLAVCALCFLFFHHAIGSRFGQALIGVRDNESRMLALGYPVFLLKLQAFVIAAAVAGLGGALMVTQNSFISPSS
MHWSQSAVLIVIVVLGGLGHRWGGVIGAAVWVSLEEVLRMSTEYWHWPLGALLIAIILFAPDGLGALCRRRAAGTGAGRV
GFFHLLRRSA
>Mature_329_residues
SSHNNAWLAPGLLLAALAAFPALAPHFGLDYYTGFVMRVLIVMLIATSLNFLMGYGGMVALGHAGFVGVGAYALVAMTEA
GITNAWALWGGAMLAASVAALLVGAVSLRTRGVYFIMITLAFAQMLYYLAVSLRAYGGDDGYNLMARPLLGFGLNADDDA
TLYWVVLAVCALCFLFFHHAIGSRFGQALIGVRDNESRMLALGYPVFLLKLQAFVIAAAVAGLGGALMVTQNSFISPSSM
HWSQSAVLIVIVVLGGLGHRWGGVIGAAVWVSLEEVLRMSTEYWHWPLGALLIAIILFAPDGLGALCRRRAAGTGAGRVG
FFHLLRRSA

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=295, Percent_Identity=28.135593220339, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 35019; Mature: 34888

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSHNNAWLAPGLLLAALAAFPALAPHFGLDYYTGFVMRVLIVMLIATSLNFLMGYGGMV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ALGHAGFVGVGAYALVAMTEAGITNAWALWGGAMLAASVAALLVGAVSLRTRGVYFIMIT
EECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAFAQMLYYLAVSLRAYGGDDGYNLMARPLLGFGLNADDDATLYWVVLAVCALCFLFFHH
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AIGSRFGQALIGVRDNESRMLALGYPVFLLKLQAFVIAAAVAGLGGALMVTQNSFISPSS
HHHHHHHHHHCCEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
MHWSQSAVLIVIVVLGGLGHRWGGVIGAAVWVSLEEVLRMSTEYWHWPLGALLIAIILFA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
PDGLGALCRRRAAGTGAGRVGFFHLLRRSA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SSHNNAWLAPGLLLAALAAFPALAPHFGLDYYTGFVMRVLIVMLIATSLNFLMGYGGMV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ALGHAGFVGVGAYALVAMTEAGITNAWALWGGAMLAASVAALLVGAVSLRTRGVYFIMIT
EECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LAFAQMLYYLAVSLRAYGGDDGYNLMARPLLGFGLNADDDATLYWVVLAVCALCFLFFHH
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHH
AIGSRFGQALIGVRDNESRMLALGYPVFLLKLQAFVIAAAVAGLGGALMVTQNSFISPSS
HHHHHHHHHHCCEECCCCCEEEECHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
MHWSQSAVLIVIVVLGGLGHRWGGVIGAAVWVSLEEVLRMSTEYWHWPLGALLIAIILFA
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
PDGLGALCRRRAAGTGAGRVGFFHLLRRSA
CCCHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]