Click here to switch to the map view.

The map label for this gene is pheT [H]

Identifier: 119897375

GI number: 119897375

Start: 1166949

End: 1169339

Strand: Direct

Name: pheT [H]

Synonym: azo1084

Alternate gene names: 119897375

Gene position: 1166949-1169339 (Clockwise)

Preceding gene: 119897374

Following gene: 119897376

Centisome position: 26.67

GC content: 65.91

Gene sequence:

>2391_bases
ATGCAATTCTCCGAACAGTGGTTGCGCACCTTCGTCAACCCGCCGCTCGATAGCGATGCGCTCGGCCACCTGCTGACCAT
GGCGGGTCTGGAGGTCGAGGAGGCGCAGCCGGTCGCCCCCGCGTTTTCAGGCATCGTCGTGGCCTCGATCATCGAGGCCG
AGAAGCATCCCAACGCGGACAAGCTGCGTGTCTGCAAGGTGGATGCGGGCACCGGCGAACTGCTGCAGATCGTGTGCGGT
GCGCCGAATGCCGCCGCCGGCATGAAGGTGCCCTGCGCCCTGGTGGGCGCGCAACTGCCTGGCGATTTCGCCATCAAGGC
TGCAAAGCTCCGTGGCGTCGAATCGTTCGGCATGCTGTGCTCGGCTCGCGAGCTGGGCATCTCCGAAGATCACGGCGGCT
TGCTTGAACTGCCGGCCGATGCCCCCGTGGGGTGCGATATCCGGGCGTATCTGGCGCTCGACGACACGCTATTCACCATC
AAGCTGACGCCCAACCGCGCGGATTGCCTGAGTCTGGCCGGGGTTGCACGCGAGGTGGCGGCGATCACCGCGGCCGAGTT
GTCGTTGCCTGCCGTGCAGCCCGTCGAGCCGGTTACGGATGCCAGGAGAGCCGTCGCGTTGGACGTGCCGGCCGCGTGCC
CCCGTTACTGTGGTCGTGTCCTGCGTGGCGTTGACGCTCGCGCGCAAACGCCGGACTGGATGAAGCAACGCCTGCAGCGC
AGTGGATTGCGCCCGATCAGTGCGCTGGTCGACATCACGAACTACGTCATGCTCGAACTCGGCCAGCCGCTGCATGCCTT
CGACGACGCTCGCATCAGCGGAAGCGTCCATGTCCGGCTTGCTTTGCCTGGCGAGCAGCTCCTGTTGCTGAATGGTCAGA
CCGTGTCCCTGACGCCGGACAGCCTCGTCATCGCCGATGAGGATCGTGTTCTTGCATTGGCGGGGGTCATGGGTGGAGAG
GAGTGCGGTGTCACCCTCGACACCCGCGACGTCTTCCTTGAAAGCGCATTCTTCGCACCCGCGGGGATCGCCGGGCGCGC
GCGTTCCTACGGGTTTTCCTCGGATGCCGCCCACCGCTTCGAGCGTGGCGTGGACTTCGCCCTCGGCCCCCGGGCGATCG
AGCGCGCGACACGCCTGATCCTCGACATCTGCGGCGGCGAGGCAGGGCCTACGGTGGTGGCGGAAGTGGCTTCGGCGCTG
CCCGAACGGCCGGCCGTGCGGCTGCGGCCGGAGCGTGCCCGACGCCTTCTTGGCGTCACCCTGGATGACGGACAGATGCA
GGCGCTGCTGGAGCGGGTGCATCTCAACGTGGTGCCCAGTGGTAGCGACCTCCTCGTGACGCCGCCGTCCTTCCGCTTCG
ACGTGGAGATCGAAGAAGACCTTATCGAAGAGCTGGCGCGGCTGCACGGTTACGACAACATCCCGGCGGTGCCTCCGCAG
GGGCAGTTGATGATGCTCGAGCGCTCCGAGTCCGCGCGCTCGCCCTGGACGGTCCGCCACCTGCTCGCAGCACGGGGATA
CCAGGAAGTGGTGAATTTCGCGTTCGTCGAGGAGGCTTGGGAGCGGGATTTCTGCGCCAACGAAGATCCCATCCGTCTCG
CCAACCCGATCGCCAGTCAGATGAGCGTGATGCGCTCGAGTCTGGTGCCGGGGCTGGTGGCAAACCTCGCAACCAATCGG
AAGCGCCAGCAACAGCGCGTGCGCGTGTTCGAAGTCGGACGCTGCTTTGAACGCAAGGCCGACGGCGAGCCGGTCGCGGG
GTTTCATCAGCCGGTTCGCATCGCCGCGCTGGCGGCGGGCGGCGTTGTGCCCGAGCAATGGGGAGAGCGCGAGCGTCAGG
TCGACTTCTACGACGTGAAGGGCGATCTTGAGGCCCTGTTTGCGCCTCGTCTGCTGAGCTTCGAGCCGCTGTCCCACCCC
GCGCTCCACCCGGGTCGGGCTGCCGCGGTGCTGCTCGACGGGCGGCGGATCGGGTGGCTTGGCGAAGTGCATCCGGTTTG
GGTGCAGCGCTACGACGTCGGTTCGGCACCGGTGGTTTTCGAGGTCGACCTCGATTCGGCGCTCGAAAGCCGTTTGCCGA
GTTTCGGCGGTGTCTCCCGGATGCCTGCGGTAACGCGCGACCTGGCGCTGGTCGTGGCGGCGGAGGTGCCGGCTGCACGG
ATTCTGGAGGTGCTGAAAGCTGCCGCGGGGCCTCTCGTAACCGAGATTTCGCTGTTCGATCTGTACCATGGTAAAGGCAT
TGATCCGGACAAGAAGAGCCTTGCTTTCAGGGTGTTGATGCAAGATACTCATCGGACTCTTGAAGACGCCGAAGTTGATG
CCGCCGTGGCTGCGCTAGTGCAACAGGCCGAGCTCGTGTTCGGCGCGCGACTGCGGGGGGCAGTTGAATGA

Upstream 100 bases:

>100_bases
GGGGCCGGACCGACTCACGATGTTGCGCTACGGCGTCAACGACCTGCGCCTGTTCTTCGAAGGCGATCTGCGCTTCTTGA
GCCAATTCAGGTAATCGAAC

Downstream 100 bases:

>100_bases
ACGTTACCTTGACGAAGGCCGAGTTGGCCGACCTGCTGTTCGAGCGGGTCGGGCTCAACAAGCGCGAGGCCAAGGACATG
GTCGAGGGTTTCTTCGAGGA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 796; Mature: 796

Protein sequence:

>796_residues
MQFSEQWLRTFVNPPLDSDALGHLLTMAGLEVEEAQPVAPAFSGIVVASIIEAEKHPNADKLRVCKVDAGTGELLQIVCG
APNAAAGMKVPCALVGAQLPGDFAIKAAKLRGVESFGMLCSARELGISEDHGGLLELPADAPVGCDIRAYLALDDTLFTI
KLTPNRADCLSLAGVAREVAAITAAELSLPAVQPVEPVTDARRAVALDVPAACPRYCGRVLRGVDARAQTPDWMKQRLQR
SGLRPISALVDITNYVMLELGQPLHAFDDARISGSVHVRLALPGEQLLLLNGQTVSLTPDSLVIADEDRVLALAGVMGGE
ECGVTLDTRDVFLESAFFAPAGIAGRARSYGFSSDAAHRFERGVDFALGPRAIERATRLILDICGGEAGPTVVAEVASAL
PERPAVRLRPERARRLLGVTLDDGQMQALLERVHLNVVPSGSDLLVTPPSFRFDVEIEEDLIEELARLHGYDNIPAVPPQ
GQLMMLERSESARSPWTVRHLLAARGYQEVVNFAFVEEAWERDFCANEDPIRLANPIASQMSVMRSSLVPGLVANLATNR
KRQQQRVRVFEVGRCFERKADGEPVAGFHQPVRIAALAAGGVVPEQWGERERQVDFYDVKGDLEALFAPRLLSFEPLSHP
ALHPGRAAAVLLDGRRIGWLGEVHPVWVQRYDVGSAPVVFEVDLDSALESRLPSFGGVSRMPAVTRDLALVVAAEVPAAR
ILEVLKAAAGPLVTEISLFDLYHGKGIDPDKKSLAFRVLMQDTHRTLEDAEVDAAVAALVQQAELVFGARLRGAVE

Sequences:

>Translated_796_residues
MQFSEQWLRTFVNPPLDSDALGHLLTMAGLEVEEAQPVAPAFSGIVVASIIEAEKHPNADKLRVCKVDAGTGELLQIVCG
APNAAAGMKVPCALVGAQLPGDFAIKAAKLRGVESFGMLCSARELGISEDHGGLLELPADAPVGCDIRAYLALDDTLFTI
KLTPNRADCLSLAGVAREVAAITAAELSLPAVQPVEPVTDARRAVALDVPAACPRYCGRVLRGVDARAQTPDWMKQRLQR
SGLRPISALVDITNYVMLELGQPLHAFDDARISGSVHVRLALPGEQLLLLNGQTVSLTPDSLVIADEDRVLALAGVMGGE
ECGVTLDTRDVFLESAFFAPAGIAGRARSYGFSSDAAHRFERGVDFALGPRAIERATRLILDICGGEAGPTVVAEVASAL
PERPAVRLRPERARRLLGVTLDDGQMQALLERVHLNVVPSGSDLLVTPPSFRFDVEIEEDLIEELARLHGYDNIPAVPPQ
GQLMMLERSESARSPWTVRHLLAARGYQEVVNFAFVEEAWERDFCANEDPIRLANPIASQMSVMRSSLVPGLVANLATNR
KRQQQRVRVFEVGRCFERKADGEPVAGFHQPVRIAALAAGGVVPEQWGERERQVDFYDVKGDLEALFAPRLLSFEPLSHP
ALHPGRAAAVLLDGRRIGWLGEVHPVWVQRYDVGSAPVVFEVDLDSALESRLPSFGGVSRMPAVTRDLALVVAAEVPAAR
ILEVLKAAAGPLVTEISLFDLYHGKGIDPDKKSLAFRVLMQDTHRTLEDAEVDAAVAALVQQAELVFGARLRGAVE
>Mature_796_residues
MQFSEQWLRTFVNPPLDSDALGHLLTMAGLEVEEAQPVAPAFSGIVVASIIEAEKHPNADKLRVCKVDAGTGELLQIVCG
APNAAAGMKVPCALVGAQLPGDFAIKAAKLRGVESFGMLCSARELGISEDHGGLLELPADAPVGCDIRAYLALDDTLFTI
KLTPNRADCLSLAGVAREVAAITAAELSLPAVQPVEPVTDARRAVALDVPAACPRYCGRVLRGVDARAQTPDWMKQRLQR
SGLRPISALVDITNYVMLELGQPLHAFDDARISGSVHVRLALPGEQLLLLNGQTVSLTPDSLVIADEDRVLALAGVMGGE
ECGVTLDTRDVFLESAFFAPAGIAGRARSYGFSSDAAHRFERGVDFALGPRAIERATRLILDICGGEAGPTVVAEVASAL
PERPAVRLRPERARRLLGVTLDDGQMQALLERVHLNVVPSGSDLLVTPPSFRFDVEIEEDLIEELARLHGYDNIPAVPPQ
GQLMMLERSESARSPWTVRHLLAARGYQEVVNFAFVEEAWERDFCANEDPIRLANPIASQMSVMRSSLVPGLVANLATNR
KRQQQRVRVFEVGRCFERKADGEPVAGFHQPVRIAALAAGGVVPEQWGERERQVDFYDVKGDLEALFAPRLLSFEPLSHP
ALHPGRAAAVLLDGRRIGWLGEVHPVWVQRYDVGSAPVVFEVDLDSALESRLPSFGGVSRMPAVTRDLALVVAAEVPAAR
ILEVLKAAAGPLVTEISLFDLYHGKGIDPDKKSLAFRVLMQDTHRTLEDAEVDAAVAALVQQAELVFGARLRGAVE

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=796, Percent_Identity=47.9899497487437, Blast_Score=728, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=389, Percent_Identity=23.3933161953728, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 85861; Mature: 85861

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSEQWLRTFVNPPLDSDALGHLLTMAGLEVEEAQPVAPAFSGIVVASIIEAEKHPNAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
KLRVCKVDAGTGELLQIVCGAPNAAAGMKVPCALVGAQLPGDFAIKAAKLRGVESFGMLC
CEEEEEECCCCCHHHHEEECCCCCCCCCCCCHHHCCCCCCCCHHEEHHHHCCCHHHHHHH
SARELGISEDHGGLLELPADAPVGCDIRAYLALDDTLFTIKLTPNRADCLSLAGVAREVA
HHHHCCCCCCCCCEEEECCCCCCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHH
AITAAELSLPAVQPVEPVTDARRAVALDVPAACPRYCGRVLRGVDARAQTPDWMKQRLQR
HHHHHHCCCCCCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
SGLRPISALVDITNYVMLELGQPLHAFDDARISGSVHVRLALPGEQLLLLNGQTVSLTPD
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEECCEEEEEEECCCCEEEEEECCEEEECCC
SLVIADEDRVLALAGVMGGEECGVTLDTRDVFLESAFFAPAGIAGRARSYGFSSDAAHRF
EEEEECCCCEEEEEECCCCCCCCEEECHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHH
ERGVDFALGPRAIERATRLILDICGGEAGPTVVAEVASALPERPAVRLRPERARRLLGVT
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECHHHHHHEEEEE
LDDGQMQALLERVHLNVVPSGSDLLVTPPSFRFDVEIEEDLIEELARLHGYDNIPAVPPQ
ECCHHHHHHHHHHHEEEECCCCCEEECCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCC
GQLMMLERSESARSPWTVRHLLAARGYQEVVNFAFVEEAWERDFCANEDPIRLANPIASQ
CCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
MSVMRSSLVPGLVANLATNRKRQQQRVRVFEVGRCFERKADGEPVAGFHQPVRIAALAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
GVVPEQWGERERQVDFYDVKGDLEALFAPRLLSFEPLSHPALHPGRAAAVLLDGRRIGWL
CCCHHHHCCHHCCCEEEECCCCHHHHHCCHHCCCCCCCCCCCCCCCEEEEEECCCEECCC
GEVHPVWVQRYDVGSAPVVFEVDLDSALESRLPSFGGVSRMPAVTRDLALVVAAEVPAAR
CCCCCEEEEEECCCCCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCHHH
ILEVLKAAAGPLVTEISLFDLYHGKGIDPDKKSLAFRVLMQDTHRTLEDAEVDAAVAALV
HHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQAELVFGARLRGAVE
HHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQFSEQWLRTFVNPPLDSDALGHLLTMAGLEVEEAQPVAPAFSGIVVASIIEAEKHPNAD
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHCCCCCC
KLRVCKVDAGTGELLQIVCGAPNAAAGMKVPCALVGAQLPGDFAIKAAKLRGVESFGMLC
CEEEEEECCCCCHHHHEEECCCCCCCCCCCCHHHCCCCCCCCHHEEHHHHCCCHHHHHHH
SARELGISEDHGGLLELPADAPVGCDIRAYLALDDTLFTIKLTPNRADCLSLAGVAREVA
HHHHCCCCCCCCCEEEECCCCCCCCCEEEEEEECCEEEEEEECCCHHHHHHHHHHHHHHH
AITAAELSLPAVQPVEPVTDARRAVALDVPAACPRYCGRVLRGVDARAQTPDWMKQRLQR
HHHHHHCCCCCCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
SGLRPISALVDITNYVMLELGQPLHAFDDARISGSVHVRLALPGEQLLLLNGQTVSLTPD
CCCCHHHHHHHHHHHHHHHCCCCHHHCCCCEECCEEEEEEECCCCEEEEEECCEEEECCC
SLVIADEDRVLALAGVMGGEECGVTLDTRDVFLESAFFAPAGIAGRARSYGFSSDAAHRF
EEEEECCCCEEEEEECCCCCCCCEEECHHHHHHHHHHCCCCCCCCCHHCCCCCCHHHHHH
ERGVDFALGPRAIERATRLILDICGGEAGPTVVAEVASALPERPAVRLRPERARRLLGVT
HCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEECHHHHHHEEEEE
LDDGQMQALLERVHLNVVPSGSDLLVTPPSFRFDVEIEEDLIEELARLHGYDNIPAVPPQ
ECCHHHHHHHHHHHEEEECCCCCEEECCCCCEEEEEEHHHHHHHHHHHCCCCCCCCCCCC
GQLMMLERSESARSPWTVRHLLAARGYQEVVNFAFVEEAWERDFCANEDPIRLANPIASQ
CCEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
MSVMRSSLVPGLVANLATNRKRQQQRVRVFEVGRCFERKADGEPVAGFHQPVRIAALAAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCC
GVVPEQWGERERQVDFYDVKGDLEALFAPRLLSFEPLSHPALHPGRAAAVLLDGRRIGWL
CCCHHHHCCHHCCCEEEECCCCHHHHHCCHHCCCCCCCCCCCCCCCEEEEEECCCEECCC
GEVHPVWVQRYDVGSAPVVFEVDLDSALESRLPSFGGVSRMPAVTRDLALVVAAEVPAAR
CCCCCEEEEEECCCCCCEEEEECHHHHHHHHCCCCCCCCCCCHHHHHHEEEEECCCCHHH
ILEVLKAAAGPLVTEISLFDLYHGKGIDPDKKSLAFRVLMQDTHRTLEDAEVDAAVAALV
HHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQAELVFGARLRGAVE
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA