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The map label for this gene is pheS

Identifier: 119897374

GI number: 119897374

Start: 1165911

End: 1166942

Strand: Direct

Name: pheS

Synonym: azo1083

Alternate gene names: 119897374

Gene position: 1165911-1166942 (Clockwise)

Preceding gene: 119897373

Following gene: 119897375

Centisome position: 26.64

GC content: 63.08

Gene sequence:

>1032_bases
ATGGATAACCTGGATCAACTGGTTCAACAGGCACAAGCCGACTTTGCCGGCGTGTCCGACAGCGCCCAGCTCGAACAGGC
GAAAGCGCGCTACCTGGGCAAGAGCGGTGCGCTTACCGAGCAGCTCAAAGGGTTGGGCAAGTTGCCGCCCGAGGAAAAGC
GCGAAGCCGGCGCCGCCATCAATCGCGCCAAGACCGCGATCGAGGCAGCCCTGGAGGCGCGGCGCAATGCCCTGCGAGAA
GCGGCGCTGCTTGCCCAACTTGCGGCAGAGGCGCTTGATGTCACCTTGCCCGGAAGGGGGGCGCAGGGGGGCGGATTGCA
CCCGGTGAGCCGTACGCTCGAGCGCATCGAGATGCTGTTCCGTTCGATCGGCTTCATTGTTGCCGATGGCCCGGAAATCG
AGACCGACTGGCACAATTTCACCGCGCTCAACACGCCGGAAAACCACCCCGCCCGGTCCATGCACGACACCTTCTACCTT
GAAGGGCGTGACGATGTGCTGCTGCGTACCCACACTAGCCCGGTGCAGATCCGCACCATGCTTGCGCACGTCAAGCGCCA
TGCCGAGGCGGCCGCGATGCCGGAGATCCGGGTGATCATCCCGGGCCGGGTGTACCGGGTCGATTCAGACGCCACGCACT
CGCCGATGTTCCATCAGGTCGAAGGCCTGTGGGTTGGCGAGAAAGTCAGCTTCGCCGATCTGAAGGGCGTCATTGCCGAC
TTCTTGCGCAAGTTCTTCGAGACCGAAGACCTGCAGGTCCGCTTCCGTCCGTCGTTCTTCCCGTTCACCGAGCCGTCCGC
TGAAATCGACGTCGCGTTCATGAGCGGTCCGCTCAAGGGGCGTTGGCTGGAGATCGCCGGCTGCGGGATGGTGCATCCGA
ATGTGTTGCGCTTTGGTGGGGTCGATCCCGAGCGTTACACCGGATTCGCATTCGGCATGGGGCCGGACCGACTCACGATG
TTGCGCTACGGCGTCAACGACCTGCGCCTGTTCTTCGAAGGCGATCTGCGCTTCTTGAGCCAATTCAGGTAA

Upstream 100 bases:

>100_bases
CGCTGAACAAGCCCGGGCCAAACTTGCTGCGTAAGCTGACGCAGCCCGAAAAAAGGAGGCCTGGCCTCCTTTTTTTTTCT
GAATAGCGGACGTGTGGCAC

Downstream 100 bases:

>100_bases
TCGAACATGCAATTCTCCGAACAGTGGTTGCGCACCTTCGTCAACCCGCCGCTCGATAGCGATGCGCTCGGCCACCTGCT
GACCATGGCGGGTCTGGAGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDNLDQLVQQAQADFAGVSDSAQLEQAKARYLGKSGALTEQLKGLGKLPPEEKREAGAAINRAKTAIEAALEARRNALRE
AALLAQLAAEALDVTLPGRGAQGGGLHPVSRTLERIEMLFRSIGFIVADGPEIETDWHNFTALNTPENHPARSMHDTFYL
EGRDDVLLRTHTSPVQIRTMLAHVKRHAEAAAMPEIRVIIPGRVYRVDSDATHSPMFHQVEGLWVGEKVSFADLKGVIAD
FLRKFFETEDLQVRFRPSFFPFTEPSAEIDVAFMSGPLKGRWLEIAGCGMVHPNVLRFGGVDPERYTGFAFGMGPDRLTM
LRYGVNDLRLFFEGDLRFLSQFR

Sequences:

>Translated_343_residues
MDNLDQLVQQAQADFAGVSDSAQLEQAKARYLGKSGALTEQLKGLGKLPPEEKREAGAAINRAKTAIEAALEARRNALRE
AALLAQLAAEALDVTLPGRGAQGGGLHPVSRTLERIEMLFRSIGFIVADGPEIETDWHNFTALNTPENHPARSMHDTFYL
EGRDDVLLRTHTSPVQIRTMLAHVKRHAEAAAMPEIRVIIPGRVYRVDSDATHSPMFHQVEGLWVGEKVSFADLKGVIAD
FLRKFFETEDLQVRFRPSFFPFTEPSAEIDVAFMSGPLKGRWLEIAGCGMVHPNVLRFGGVDPERYTGFAFGMGPDRLTM
LRYGVNDLRLFFEGDLRFLSQFR
>Mature_343_residues
MDNLDQLVQQAQADFAGVSDSAQLEQAKARYLGKSGALTEQLKGLGKLPPEEKREAGAAINRAKTAIEAALEARRNALRE
AALLAQLAAEALDVTLPGRGAQGGGLHPVSRTLERIEMLFRSIGFIVADGPEIETDWHNFTALNTPENHPARSMHDTFYL
EGRDDVLLRTHTSPVQIRTMLAHVKRHAEAAAMPEIRVIIPGRVYRVDSDATHSPMFHQVEGLWVGEKVSFADLKGVIAD
FLRKFFETEDLQVRFRPSFFPFTEPSAEIDVAFMSGPLKGRWLEIAGCGMVHPNVLRFGGVDPERYTGFAFGMGPDRLTM
LRYGVNDLRLFFEGDLRFLSQFR

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=267, Percent_Identity=30.7116104868914, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI5729820, Length=294, Percent_Identity=30.6122448979592, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1788007, Length=343, Percent_Identity=59.4752186588921, Blast_Score=401, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17508957, Length=269, Percent_Identity=31.9702602230483, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=269, Percent_Identity=32.3420074349442, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI32566635, Length=264, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=370, Percent_Identity=26.4864864864865, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=94, Percent_Identity=41.4893617021277, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI18858079, Length=288, Percent_Identity=30.9027777777778, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17137424, Length=253, Percent_Identity=27.6679841897233, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_AZOSB (A1K4E5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932587.1
- ProteinModelPortal:   A1K4E5
- SMR:   A1K4E5
- STRING:   A1K4E5
- GeneID:   4609779
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1083
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   A1K4E5
- ProtClustDB:   PRK00488
- BioCyc:   ASP62928:AZO1083-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38161; Mature: 38161

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNLDQLVQQAQADFAGVSDSAQLEQAKARYLGKSGALTEQLKGLGKLPPEEKREAGAAI
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
NRAKTAIEAALEARRNALREAALLAQLAAEALDVTLPGRGAQGGGLHPVSRTLERIEMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
RSIGFIVADGPEIETDWHNFTALNTPENHPARSMHDTFYLEGRDDVLLRTHTSPVQIRTM
HHCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHH
LAHVKRHAEAAAMPEIRVIIPGRVYRVDSDATHSPMFHQVEGLWVGEKVSFADLKGVIAD
HHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHH
FLRKFFETEDLQVRFRPSFFPFTEPSAEIDVAFMSGPLKGRWLEIAGCGMVHPNVLRFGG
HHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCHHEECC
VDPERYTGFAFGMGPDRLTMLRYGVNDLRLFFEGDLRFLSQFR
CCHHHHCEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHCC
>Mature Secondary Structure
MDNLDQLVQQAQADFAGVSDSAQLEQAKARYLGKSGALTEQLKGLGKLPPEEKREAGAAI
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHH
NRAKTAIEAALEARRNALREAALLAQLAAEALDVTLPGRGAQGGGLHPVSRTLERIEMLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
RSIGFIVADGPEIETDWHNFTALNTPENHPARSMHDTFYLEGRDDVLLRTHTSPVQIRTM
HHCCEEEECCCCCCCCCCCEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHH
LAHVKRHAEAAAMPEIRVIIPGRVYRVDSDATHSPMFHQVEGLWVGEKVSFADLKGVIAD
HHHHHHHHHHHCCCCEEEEECCEEEEECCCCCCCCHHHHHCCEEECCCCCHHHHHHHHHH
FLRKFFETEDLQVRFRPSFFPFTEPSAEIDVAFMSGPLKGRWLEIAGCGMVHPNVLRFGG
HHHHHHCCCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCHHEECC
VDPERYTGFAFGMGPDRLTMLRYGVNDLRLFFEGDLRFLSQFR
CCHHHHCEEEECCCCCHHHHHHHCCCCEEEEECCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA