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The map label for this gene is trpA

Identifier: 119897339

GI number: 119897339

Start: 1127891

End: 1128706

Strand: Direct

Name: trpA

Synonym: azo1048

Alternate gene names: 119897339

Gene position: 1127891-1128706 (Clockwise)

Preceding gene: 119897338

Following gene: 119897340

Centisome position: 25.77

GC content: 68.26

Gene sequence:

>816_bases
ATGTCGAAAATCCAGACCACTTTCCAGCGCCTTCAGGCCCAGGGCCGCAAGGCGCTGATCCCGTTCATCACCGCGGGCGA
TCCCGACCCCACGCTGACGGTGCCGCTGATGCATGCGCTCGTTGCGGGCGGGGCGGACATCATCGAGTTGGGGGTCCCGT
TCTCCGACCCCATGGCTGACGGCCCGACCATCCAGCGTGCTTCCGAGCGCGCATTGGCGCAGGGCATGACCTTGCGCAAG
GTGCTGCAGGCCGTGCGCGAGTTCCGCAGCGGGGATGCCGACACCCCGGTGGTACTGATGGGTTACGCCAATCCGATCGA
GGCGATGGGCCAGCAGGCCTTTGTCGCCGCAGCCCGCGAAGCCGGCGTGGACGGCGCGCTCGTGGTGGATTACCCGCCCG
AAGAGTGCGTCGAATTCGCCGCGGCCAGCAAGGCGGCCGGGCTCGACCCGATCTTCCTGCTCGCGCCGACGTCGTCGGAA
CAGCGCTTTGCGGACGTCGCCCGGGCGGGCAGCGGCTACATCTACTACGTCTCGCTGAAGGGCGTGACCGGCGCTGGCAC
GCTGGATCTGGACGAGGTCGCGCGCCGCATTCCGCAGATCCGCGCTGCGGTGGGCATGCCGGTGGGCGTGGGGTTCGGCA
TCCGTGACGCCGAAAGCGCGCGACGGATCGGCGCCGTGGCCGATGCGGTGGTGATCGGCAGCCGCATCATCGAAGAGATC
GAACGCAGCCCGCGCGAGCAGGCCTGCAGCAATGTCACCCACTTCGTGAAGGGCATCCGCGAAGCCCTCGATACCCTGCC
CGGAGTCAAGCAATGA

Upstream 100 bases:

>100_bases
CGGGCGTGGCGACAAGGACATGCACACCGTCGCCGAACGCTCCGGCATCCAGTTCTGAACCCGTAGCGGGCCGTCCCGGC
CCGCCGCTGCCGAATCCGAC

Downstream 100 bases:

>100_bases
GCTGGCTGCAGAAACTGCTTCCGCCCAAGATCAAGCGCAGCGACGCCGCCGCGCGCAGCAAGTCCATCCCGGAAGGGCTG
TGGAGCAAGTGCCCGTCGTG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSKIQTTFQRLQAQGRKALIPFITAGDPDPTLTVPLMHALVAGGADIIELGVPFSDPMADGPTIQRASERALAQGMTLRK
VLQAVREFRSGDADTPVVLMGYANPIEAMGQQAFVAAAREAGVDGALVVDYPPEECVEFAAASKAAGLDPIFLLAPTSSE
QRFADVARAGSGYIYYVSLKGVTGAGTLDLDEVARRIPQIRAAVGMPVGVGFGIRDAESARRIGAVADAVVIGSRIIEEI
ERSPREQACSNVTHFVKGIREALDTLPGVKQ

Sequences:

>Translated_271_residues
MSKIQTTFQRLQAQGRKALIPFITAGDPDPTLTVPLMHALVAGGADIIELGVPFSDPMADGPTIQRASERALAQGMTLRK
VLQAVREFRSGDADTPVVLMGYANPIEAMGQQAFVAAAREAGVDGALVVDYPPEECVEFAAASKAAGLDPIFLLAPTSSE
QRFADVARAGSGYIYYVSLKGVTGAGTLDLDEVARRIPQIRAAVGMPVGVGFGIRDAESARRIGAVADAVVIGSRIIEEI
ERSPREQACSNVTHFVKGIREALDTLPGVKQ
>Mature_270_residues
SKIQTTFQRLQAQGRKALIPFITAGDPDPTLTVPLMHALVAGGADIIELGVPFSDPMADGPTIQRASERALAQGMTLRKV
LQAVREFRSGDADTPVVLMGYANPIEAMGQQAFVAAAREAGVDGALVVDYPPEECVEFAAASKAAGLDPIFLLAPTSSEQ
RFADVARAGSGYIYYVSLKGVTGAGTLDLDEVARRIPQIRAAVGMPVGVGFGIRDAESARRIGAVADAVVIGSRIIEEIE
RSPREQACSNVTHFVKGIREALDTLPGVKQ

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=250, Percent_Identity=34.8, Blast_Score=140, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6321412, Length=260, Percent_Identity=38.0769230769231, Blast_Score=184, Evalue=1e-47,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_AZOSB (A1K4B0)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932552.1
- ProteinModelPortal:   A1K4B0
- SMR:   A1K4B0
- STRING:   A1K4B0
- GeneID:   4610030
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1048
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   A1K4B0
- ProtClustDB:   PRK13111
- BioCyc:   ASP62928:AZO1048-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28616; Mature: 28485

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIQTTFQRLQAQGRKALIPFITAGDPDPTLTVPLMHALVAGGADIIELGVPFSDPMAD
CCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRASERALAQGMTLRKVLQAVREFRSGDADTPVVLMGYANPIEAMGQQAFVAAARE
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGVDGALVVDYPPEECVEFAAASKAAGLDPIFLLAPTSSEQRFADVARAGSGYIYYVSLK
CCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEC
GVTGAGTLDLDEVARRIPQIRAAVGMPVGVGFGIRDAESARRIGAVADAVVIGSRIIEEI
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERSPREQACSNVTHFVKGIREALDTLPGVKQ
HHCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SKIQTTFQRLQAQGRKALIPFITAGDPDPTLTVPLMHALVAGGADIIELGVPFSDPMAD
CHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRASERALAQGMTLRKVLQAVREFRSGDADTPVVLMGYANPIEAMGQQAFVAAARE
CCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
AGVDGALVVDYPPEECVEFAAASKAAGLDPIFLLAPTSSEQRFADVARAGSGYIYYVSLK
CCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEEC
GVTGAGTLDLDEVARRIPQIRAAVGMPVGVGFGIRDAESARRIGAVADAVVIGSRIIEEI
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ERSPREQACSNVTHFVKGIREALDTLPGVKQ
HHCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA