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The map label for this gene is rimO

Identifier: 119897316

GI number: 119897316

Start: 1101080

End: 1102411

Strand: Direct

Name: rimO

Synonym: azo1025

Alternate gene names: 119897316

Gene position: 1101080-1102411 (Clockwise)

Preceding gene: 119897315

Following gene: 119897317

Centisome position: 25.16

GC content: 63.44

Gene sequence:

>1332_bases
ATGAGCAATATCAAGACCCAGGACGTGCCGCGCGTCGGCTTCGTATCCCTCGGGTGCCCGAAGGCGACTTCGGATTCGGA
ACACATCCTCACGCGCCTGCGGGCCGAAGGGTACGAAATCTCCGGCAGCTATGACGCCGCGGATCTGGTCGTGGTGAATA
CCTGCGGCTTCATCGACGCGGCCGTCGAGGAGTCGCTGGATGCGATCGGCGAGGCCCTGGCGGAGAACGGTCGGGTGATC
GTCACCGGTTGTCTTGGCGCAAAGGACGACGTCATCCTTGCGGCTCATCCCCAGGTTCTTGCGGTGACCGGGCCGCATGC
CACGGAAGAGGTGATGCAGGCTGTCCATCGCCACCTTCCCAAGCCGCACGACCCGTTTTCCGACCTGGTGCCACCGCAGG
GGATCCGGCTCACGCCGCAGCATTACGCGTACCTGAAGATTTCCGAGGGCTGCAACCATCGGTGCACCTTCTGCATCATC
CCCTCGATGCGCGGCGATCTGGTAAGCCGGCCCATTCACGATGTCATGCGGGAGGCCGAGGCGCTCGCCGATGCTGGCGT
GAAGGAGTTGCTGGTCATTTCCCAGGACACCTCGGCCTACGGCGTCGACGTCAAGTACCGCACCGGCTTCTGGGGCGGAA
AGCCCGTGAAGACGAGGCTTTACGATCTTGCCAACGCCCTGGGCGAACTGGGCATCTGGATCCGGATGCACTACGTCTAC
CCATATCCCAGTGTCGATGACCTGATTCCGCTGATGGCGGAAGGAAAGATACTGCCCTATCTCGACGTGCCCTTCCAGCA
CGCGAGCCCGCGCATCCTCAAGGCGATGAAGCGCCCCGCCAATGCGGAAAACGTGCTGGAGCGCGTCCGCAAGTGGCGGG
AGATCTGCCCGGACCTTACGATCCGGTCGACCTTCATCACCGGCTTTCCGGGGGAGACGGAGGAGGACTTCGAGCAACTC
CTGCAGTTTCTGGAGGCGGCGCAGCTCGACCGGGTGGGTGCGTTTGCCTACTCGCCGGTGGAAGGCGCCGCAGCCAACGA
CTTGCCGGATGCCGTCCCCGATGAGGTCCGTGAAGAGCGCCGCGCGCGCCTGATGGATTTCCAGGAAGACATCTCGACGC
AGCGCCTTGAGGCCAAGATTGGCCGTGAGATGACGGTACTGGTGGATGATGTGGACGAGGAGGGGGCGCTTGCGCGTTCG
CCCGGAGATGCGCCTGAAATCGACGGTCTCGTCGTGATCCCGGACGGCGAGGGACTGGCGCCGGGAGACTTCGTCCGTGT
CCGCATCACAGATTGCGATATCCATGATCTCTACGCGGAGCGGGTGGTCTAG

Upstream 100 bases:

>100_bases
GACGCCACCGCTGCCGGCAAGGGCAGTACCGACAAGTAACTCAAAGGCAGCCGTTCAAGGCTCCGGTATCCGGGGCGGCC
GCGCGACAGGCACGAGCAAG

Downstream 100 bases:

>100_bases
TTTCGCGTGAAGGAGGGCGTCTGGACGGTCTGCCCAGTCTGAACCCATCCGAGCGCCCCGTCATTGGTCTGGCGCTGGGG
AGCGGTGCAGCCCGCGGTTG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSNIKTQDVPRVGFVSLGCPKATSDSEHILTRLRAEGYEISGSYDAADLVVVNTCGFIDAAVEESLDAIGEALAENGRVI
VTGCLGAKDDVILAAHPQVLAVTGPHATEEVMQAVHRHLPKPHDPFSDLVPPQGIRLTPQHYAYLKISEGCNHRCTFCII
PSMRGDLVSRPIHDVMREAEALADAGVKELLVISQDTSAYGVDVKYRTGFWGGKPVKTRLYDLANALGELGIWIRMHYVY
PYPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAMKRPANAENVLERVRKWREICPDLTIRSTFITGFPGETEEDFEQL
LQFLEAAQLDRVGAFAYSPVEGAAANDLPDAVPDEVREERRARLMDFQEDISTQRLEAKIGREMTVLVDDVDEEGALARS
PGDAPEIDGLVVIPDGEGLAPGDFVRVRITDCDIHDLYAERVV

Sequences:

>Translated_443_residues
MSNIKTQDVPRVGFVSLGCPKATSDSEHILTRLRAEGYEISGSYDAADLVVVNTCGFIDAAVEESLDAIGEALAENGRVI
VTGCLGAKDDVILAAHPQVLAVTGPHATEEVMQAVHRHLPKPHDPFSDLVPPQGIRLTPQHYAYLKISEGCNHRCTFCII
PSMRGDLVSRPIHDVMREAEALADAGVKELLVISQDTSAYGVDVKYRTGFWGGKPVKTRLYDLANALGELGIWIRMHYVY
PYPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAMKRPANAENVLERVRKWREICPDLTIRSTFITGFPGETEEDFEQL
LQFLEAAQLDRVGAFAYSPVEGAAANDLPDAVPDEVREERRARLMDFQEDISTQRLEAKIGREMTVLVDDVDEEGALARS
PGDAPEIDGLVVIPDGEGLAPGDFVRVRITDCDIHDLYAERVV
>Mature_442_residues
SNIKTQDVPRVGFVSLGCPKATSDSEHILTRLRAEGYEISGSYDAADLVVVNTCGFIDAAVEESLDAIGEALAENGRVIV
TGCLGAKDDVILAAHPQVLAVTGPHATEEVMQAVHRHLPKPHDPFSDLVPPQGIRLTPQHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIHDVMREAEALADAGVKELLVISQDTSAYGVDVKYRTGFWGGKPVKTRLYDLANALGELGIWIRMHYVYP
YPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAMKRPANAENVLERVRKWREICPDLTIRSTFITGFPGETEEDFEQLL
QFLEAAQLDRVGAFAYSPVEGAAANDLPDAVPDEVREERRARLMDFQEDISTQRLEAKIGREMTVLVDDVDEEGALARSP
GDAPEIDGLVVIPDGEGLAPGDFVRVRITDCDIHDLYAERVV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=477, Percent_Identity=26.2054507337526, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI28872782, Length=477, Percent_Identity=26.2054507337526, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI93277076, Length=402, Percent_Identity=26.1194029850746, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=66.5914221218962, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1786882, Length=435, Percent_Identity=28.9655172413793, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=482, Percent_Identity=26.3485477178423, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71996771, Length=291, Percent_Identity=28.5223367697594, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=25.6842105263158, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI19922432, Length=448, Percent_Identity=26.3392857142857, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_AZOSB (A1K487)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932529.1
- ProteinModelPortal:   A1K487
- SMR:   A1K487
- STRING:   A1K487
- GeneID:   4609543
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo1025
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   A1K487
- ProtClustDB:   PRK14862
- BioCyc:   ASP62928:AZO1025-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48792; Mature: 48661

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIKTQDVPRVGFVSLGCPKATSDSEHILTRLRAEGYEISGSYDAADLVVVNTCGFIDA
CCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCCEEEEEECCCHHHH
AVEESLDAIGEALAENGRVIVTGCLGAKDDVILAAHPQVLAVTGPHATEEVMQAVHRHLP
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHCC
KPHDPFSDLVPPQGIRLTPQHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIHDVMREAE
CCCCCHHHCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
ALADAGVKELLVISQDTSAYGVDVKYRTGFWGGKPVKTRLYDLANALGELGIWIRMHYVY
HHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEC
PYPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAMKRPANAENVLERVRKWREICPDLT
CCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
IRSTFITGFPGETEEDFEQLLQFLEAAQLDRVGAFAYSPVEGAAANDLPDAVPDEVREER
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHH
RARLMDFQEDISTQRLEAKIGREMTVLVDDVDEEGALARSPGDAPEIDGLVVIPDGEGLA
HHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PGDFVRVRITDCDIHDLYAERVV
CCCEEEEEEECCCHHHHHHHHCC
>Mature Secondary Structure 
SNIKTQDVPRVGFVSLGCPKATSDSEHILTRLRAEGYEISGSYDAADLVVVNTCGFIDA
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCCCEEEEEECCCHHHH
AVEESLDAIGEALAENGRVIVTGCLGAKDDVILAAHPQVLAVTGPHATEEVMQAVHRHLP
HHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEECCCEEEEECCCHHHHHHHHHHHHCC
KPHDPFSDLVPPQGIRLTPQHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIHDVMREAE
CCCCCHHHCCCCCCEEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
ALADAGVKELLVISQDTSAYGVDVKYRTGFWGGKPVKTRLYDLANALGELGIWIRMHYVY
HHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEEC
PYPSVDDLIPLMAEGKILPYLDVPFQHASPRILKAMKRPANAENVLERVRKWREICPDLT
CCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
IRSTFITGFPGETEEDFEQLLQFLEAAQLDRVGAFAYSPVEGAAANDLPDAVPDEVREER
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHHH
RARLMDFQEDISTQRLEAKIGREMTVLVDDVDEEGALARSPGDAPEIDGLVVIPDGEGLA
HHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCCC
PGDFVRVRITDCDIHDLYAERVV
CCCEEEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA