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The map label for this gene is phaC [H]

Identifier: 119897312

GI number: 119897312

Start: 1096743

End: 1098485

Strand: Direct

Name: phaC [H]

Synonym: azo1021

Alternate gene names: 119897312

Gene position: 1096743-1098485 (Clockwise)

Preceding gene: 119897310

Following gene: 119897313

Centisome position: 25.06

GC content: 64.26

Gene sequence:

>1743_bases
ATGTCCAATTCGAACGAAGGGCAGGCGGCTGCGGCCATGCAGGCGATGCTCCTGGCGGGCCAGGCCATGGCCCAGACATT
TTTCGACAATATCGCCAAACAGCACTCGGCCGTGGAGGATTCCGGCGGGGGAGGCGTGCCGCCGCTGCCTCTGCCCGAAA
CCGAAACCTTCTCCCGTCTCCAGCACGATTTCGCCGAGAAGCACCTCGCCCTGTGGTCGTCGATGCTCGGGCGCAAACCG
CAGCAGCCGGTTGCCCCCGTTGTGAAGGCGGTCGAGGCAGACAAGCGCTTCAACGCCCCGGAATGGGCCGACAGCCCGGT
GTTCGACTACGTCCGCCAGGCCTACATGTTGAACGCGGGTTTCCTGAAGGAGGTTGCGGATGCCTTGCCGATTGCCGATG
GCCGCGCGAAATCCCGCATCCAGTTCCTGACCCGGCAGTACATCGATGCGCTCGCGCCGACCAACTTTGCTGCGACCAAT
CCCGAGTTCATCCGTACCGCACTCGAAACCCGCGGCGAAAGCATCACGACCGGGGTCAGGAACCTGATTGCCGACCTCGA
GAAGGGCCGGATCTCGATGACCGACGACGAGGCGTTCGAGGTCGGCCGCAACCTGGCGGTGTCGCCGGGTGCGGTGATCT
ATCAGAACGAACTCATCCAGCTGATCCAGTACGCGCCGCTGACCGACAAGGTGGCCCAGGTTCCGCTGCTGATCGTGCCG
CCCTGCATCAACAAGTTCTACATCCTCGATCTCCAGCCGGAGAATTCGCTGGTGCGCTTCCTCGTCGAGCAAGGGCACAC
GGTGTTCCTGGTGTCGTGGAAGAACGTCCGGCCGGACGAGGGCAACTTCACCTGGGATGACTATCTCGACAAGGGCGTGT
TCGCCGCGCTCGACGTCGTGCGCGCGGTCACGCGCGTCAAAAAGCCGAATGTGCTGGGCTTCTGCGTCGGCGGCACCATT
CTGACCTCGGCACTCGCGGTCGCCCGCGCGCGCGGGGAGGACCCGGTGCAGAGCCTGACCCTGATGACCACGCTGCTCGA
TTTCGCCGATGCGGGCGAACTGGGTTGCCTGGTGGACGAGGCGAGCGTGGCGGCGCGCGAGGCCGCGATTGGCAAGGGCG
GCATCCTGCGGGGACAGGAACTAGCCAACGTGTTTTCTTCGCTGCGCGCCAACGATCTCGTCTGGCAATACGTGGTCGGC
AACTATCTGAAGGGGCAGAAGCCGCAGGCCTTCGACCTGCTGTACTGGAATTCGGACTCCACCAACCTGCCGGGGCCGTT
CCTGACCTGGTACCTGCGCAACATGTATCTGGAGAACAACCTGCGCGTGCCGGGCAAACTCAAGATGCTTGGCCACAAGG
TCGACCTCGGCAAGGTGGATGCGCCTGCCTATGTGATGGCCGCGCGCGAGGACCACATCGTTCCGTGGGCGAGTTCCTAT
CTCGGTCGCCACCTGCTTGGGGGAGAAACGACCTTCGTGCTGGGGGCCAGCGGTCATATCGCCGGGGCTATCAACCCGGC
CTCGAAGAATCGCCGCAGCTACTGGTCCAGCGACGCGAAGAGCGCGGACCCCGAAGAGTGGCTGGAGCAGGCGGTCGAGA
ACAAAGGGTCGTGGTGGCTGCACTGGGCGCAGTGGTTGACCGCGCACCGGGGGAAGACCGTGGCCGCTCGCGGTCGGTTG
GGCAATGCGAAGTACGCGCCGATCGAGCCTGCTCCTGGGCGTTATGTGAAGGACAAGGCCTAG

Upstream 100 bases:

>100_bases
CGATCAGGTACATGCCTCGATTGTACTTCCGCGGGCTGCGCGGACGAGGCGACGACGGAAACTATCGGGGCTGCTGGCGG
CCTCGGCTTAGGAGGGGCAG

Downstream 100 bases:

>100_bases
CGCGCTTCCAGGCGGAAAACCGGGATCTGGTCGACTGACGACGACGGGGAGGGCGCCGTCAGTCGAGCTTGCTACCCCCC
CGAGACAACACAAGATAGAG

Product: polyhydroxyalkanoate synthase

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 580; Mature: 579

Protein sequence:

>580_residues
MSNSNEGQAAAAMQAMLLAGQAMAQTFFDNIAKQHSAVEDSGGGGVPPLPLPETETFSRLQHDFAEKHLALWSSMLGRKP
QQPVAPVVKAVEADKRFNAPEWADSPVFDYVRQAYMLNAGFLKEVADALPIADGRAKSRIQFLTRQYIDALAPTNFAATN
PEFIRTALETRGESITTGVRNLIADLEKGRISMTDDEAFEVGRNLAVSPGAVIYQNELIQLIQYAPLTDKVAQVPLLIVP
PCINKFYILDLQPENSLVRFLVEQGHTVFLVSWKNVRPDEGNFTWDDYLDKGVFAALDVVRAVTRVKKPNVLGFCVGGTI
LTSALAVARARGEDPVQSLTLMTTLLDFADAGELGCLVDEASVAAREAAIGKGGILRGQELANVFSSLRANDLVWQYVVG
NYLKGQKPQAFDLLYWNSDSTNLPGPFLTWYLRNMYLENNLRVPGKLKMLGHKVDLGKVDAPAYVMAAREDHIVPWASSY
LGRHLLGGETTFVLGASGHIAGAINPASKNRRSYWSSDAKSADPEEWLEQAVENKGSWWLHWAQWLTAHRGKTVAARGRL
GNAKYAPIEPAPGRYVKDKA

Sequences:

>Translated_580_residues
MSNSNEGQAAAAMQAMLLAGQAMAQTFFDNIAKQHSAVEDSGGGGVPPLPLPETETFSRLQHDFAEKHLALWSSMLGRKP
QQPVAPVVKAVEADKRFNAPEWADSPVFDYVRQAYMLNAGFLKEVADALPIADGRAKSRIQFLTRQYIDALAPTNFAATN
PEFIRTALETRGESITTGVRNLIADLEKGRISMTDDEAFEVGRNLAVSPGAVIYQNELIQLIQYAPLTDKVAQVPLLIVP
PCINKFYILDLQPENSLVRFLVEQGHTVFLVSWKNVRPDEGNFTWDDYLDKGVFAALDVVRAVTRVKKPNVLGFCVGGTI
LTSALAVARARGEDPVQSLTLMTTLLDFADAGELGCLVDEASVAAREAAIGKGGILRGQELANVFSSLRANDLVWQYVVG
NYLKGQKPQAFDLLYWNSDSTNLPGPFLTWYLRNMYLENNLRVPGKLKMLGHKVDLGKVDAPAYVMAAREDHIVPWASSY
LGRHLLGGETTFVLGASGHIAGAINPASKNRRSYWSSDAKSADPEEWLEQAVENKGSWWLHWAQWLTAHRGKTVAARGRL
GNAKYAPIEPAPGRYVKDKA
>Mature_579_residues
SNSNEGQAAAAMQAMLLAGQAMAQTFFDNIAKQHSAVEDSGGGGVPPLPLPETETFSRLQHDFAEKHLALWSSMLGRKPQ
QPVAPVVKAVEADKRFNAPEWADSPVFDYVRQAYMLNAGFLKEVADALPIADGRAKSRIQFLTRQYIDALAPTNFAATNP
EFIRTALETRGESITTGVRNLIADLEKGRISMTDDEAFEVGRNLAVSPGAVIYQNELIQLIQYAPLTDKVAQVPLLIVPP
CINKFYILDLQPENSLVRFLVEQGHTVFLVSWKNVRPDEGNFTWDDYLDKGVFAALDVVRAVTRVKKPNVLGFCVGGTIL
TSALAVARARGEDPVQSLTLMTTLLDFADAGELGCLVDEASVAAREAAIGKGGILRGQELANVFSSLRANDLVWQYVVGN
YLKGQKPQAFDLLYWNSDSTNLPGPFLTWYLRNMYLENNLRVPGKLKMLGHKVDLGKVDAPAYVMAAREDHIVPWASSYL
GRHLLGGETTFVLGASGHIAGAINPASKNRRSYWSSDAKSADPEEWLEQAVENKGSWWLHWAQWLTAHRGKTVAARGRLG
NAKYAPIEPAPGRYVKDKA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 63679; Mature: 63548

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSNEGQAAAAMQAMLLAGQAMAQTFFDNIAKQHSAVEDSGGGGVPPLPLPETETFSRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
QHDFAEKHLALWSSMLGRKPQQPVAPVVKAVEADKRFNAPEWADSPVFDYVRQAYMLNAG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHH
FLKEVADALPIADGRAKSRIQFLTRQYIDALAPTNFAATNPEFIRTALETRGESITTGVR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
NLIADLEKGRISMTDDEAFEVGRNLAVSPGAVIYQNELIQLIQYAPLTDKVAQVPLLIVP
HHHHHHHCCCEECCCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHCCEEEEC
PCINKFYILDLQPENSLVRFLVEQGHTVFLVSWKNVRPDEGNFTWDDYLDKGVFAALDVV
CCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
RAVTRVKKPNVLGFCVGGTILTSALAVARARGEDPVQSLTLMTTLLDFADAGELGCLVDE
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEECH
ASVAAREAAIGKGGILRGQELANVFSSLRANDLVWQYVVGNYLKGQKPQAFDLLYWNSDS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
TNLPGPFLTWYLRNMYLENNLRVPGKLKMLGHKVDLGKVDAPAYVMAAREDHIVPWASSY
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHH
LGRHLLGGETTFVLGASGHIAGAINPASKNRRSYWSSDAKSADPEEWLEQAVENKGSWWL
HHHHHCCCCEEEEEECCCCEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEE
HWAQWLTAHRGKTVAARGRLGNAKYAPIEPAPGRYVKDKA
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNSNEGQAAAAMQAMLLAGQAMAQTFFDNIAKQHSAVEDSGGGGVPPLPLPETETFSRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHH
QHDFAEKHLALWSSMLGRKPQQPVAPVVKAVEADKRFNAPEWADSPVFDYVRQAYMLNAG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCHH
FLKEVADALPIADGRAKSRIQFLTRQYIDALAPTNFAATNPEFIRTALETRGESITTGVR
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHH
NLIADLEKGRISMTDDEAFEVGRNLAVSPGAVIYQNELIQLIQYAPLTDKVAQVPLLIVP
HHHHHHHCCCEECCCHHHHHHCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHCCEEEEC
PCINKFYILDLQPENSLVRFLVEQGHTVFLVSWKNVRPDEGNFTWDDYLDKGVFAALDVV
CCCCCEEEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
RAVTRVKKPNVLGFCVGGTILTSALAVARARGEDPVQSLTLMTTLLDFADAGELGCLVDE
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCEEECH
ASVAAREAAIGKGGILRGQELANVFSSLRANDLVWQYVVGNYLKGQKPQAFDLLYWNSDS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCC
TNLPGPFLTWYLRNMYLENNLRVPGKLKMLGHKVDLGKVDAPAYVMAAREDHIVPWASSY
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCHHHH
LGRHLLGGETTFVLGASGHIAGAINPASKNRRSYWSSDAKSADPEEWLEQAVENKGSWWL
HHHHHCCCCEEEEEECCCCEEECCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEE
HWAQWLTAHRGKTVAARGRLGNAKYAPIEPAPGRYVKDKA
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]