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The map label for this gene is tilS

Identifier: 119897207

GI number: 119897207

Start: 981412

End: 982758

Strand: Direct

Name: tilS

Synonym: azo0916

Alternate gene names: 119897207

Gene position: 981412-982758 (Clockwise)

Preceding gene: 119897206

Following gene: 119897210

Centisome position: 22.43

GC content: 72.68

Gene sequence:

>1347_bases
GTGGCCGTGGAACTCGCCGGACCGTTTGCCGAGGTGCTGCGCGCCGCGGCGATCGGTCCGGCGAGCCGTTTGTGCTGCGC
GCTGTCGGGAGGCGTGGATTCGGTCGTCACCCTCGATCTGCTGACGCGGCTGCAACCCCGCTTCGGTTTCACCCTCACCG
CGGTGCATGTGCACCACGGTCTCAGTCCGCATGCCGACGCCTGGGCGCAGTTCTGTGCGCGGCTGTGTGCCGCGCGCGGG
CTTACGCTGGCGATACGGCGGGTCGAGGTGCCCACGGACACCGGGCAGGGGCTGGAGTCGGCCGCGCGTGCGCGGCGCCA
TGCAGAACTCGTCGCCCTGCCGTGCGACTGGCTTGTTTTCGGCCACCATCAGGACGATCAGGCCGAGACCGTGCTGTTCC
GCCTGTTCCGCGGCAGCGGCCTGCGGGGACTGGGGGCGATGGCGGCGGTGGAGCCCGGTCAGCACGCAATGCCGGGCAAG
CTGCGCCCGTTGCTCGACATCGGCCGCGCCGGGATCGTGGCCTATGCGCGGGCCGCCGGCCTGGAGTGGATCGAGGACGA
AAGCAACGCCGACTGCCGCTTCACCCGCAACGCCTTGCGGCACAAGGTGCTTCCGGTCGTCCAGGCGCAGTTTCCGGCTG
TGGCGCCCACGCTGGCGCGGACCGCGGCGCTGCTGCGCGAAGGCGCCGACCTGCTGGACGATCTCGCGCGGCTGGACGAG
AACGCGTGTGGCGGTCCGGTGCTTGCTGCGGACGTCTTCGCGTCGTTGCCGGCGGCGCGGGCCGCCAATCTGTTGCGTTG
GCAGACCGCGCGGATGGGGGCGCGGGCGCCGTCGAGGGCCCGCCTGGGCGAGACGCTCCGCCAGCTGCGCGAGGCACCGG
GCCCGCTACGTCTCCCGCTGGGTGATCTGTGGTGTTGCGCTTATCAGGGCCGGGTGTGGCTGGAGCGTGATGATGAAGCG
CCGCTGCGCGCGCACCTTTGGTGCGGCGAATCCAGCCTGGGTTGGGGGGCGGGGCAGGTGAGGCTGAGTCAGGGGGGCGG
GGGTGCCGCGCTTCGCGTGGCAGCCGGCGAGGCGATGCTGGCGCCGCCCGCACCCGGTCTGCGCATGCGCCTCGGGCCAG
GGCGGCCCACGCGCAGCTTCAAGAACCTGTGCCAGGAGGCGGGCATACCGCCGTGGCTGCGCCCGCGGCTGCCGGTGTTG
TGGGTGGCGGGCGAGCCGGCCTGGATCGGCGGTATTGGCATCGCCGCGCAGTTCCAGTGCGCGGAGGGGGAGCAGGGCGT
GGTGCCGGCGTGGGTGCCGGCGTCAGCTGCGCAGCAGGGTCAGCATCTGGGCCAGTTCGACCGCTGA

Upstream 100 bases:

>100_bases
AGGTCGACACGCTGTCACCGTCGGAACTCGTCGAACAGCGGATGGAAAAGCTGATGGGTTACGGCCGCTTCAAGGAAATC
GCCGCCTGACTCTCCCGGCC

Downstream 100 bases:

>100_bases
GCGCACTTCCATCTTGTCGAATACCCGCGAGCGGTGGGCTTCGACGGTGCGCATCGAGATCTGCAGGTCGTCGGCGATGA
CCTTGTTGAATTTGCCTTCG

Product: putative tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MAVELAGPFAEVLRAAAIGPASRLCCALSGGVDSVVTLDLLTRLQPRFGFTLTAVHVHHGLSPHADAWAQFCARLCAARG
LTLAIRRVEVPTDTGQGLESAARARRHAELVALPCDWLVFGHHQDDQAETVLFRLFRGSGLRGLGAMAAVEPGQHAMPGK
LRPLLDIGRAGIVAYARAAGLEWIEDESNADCRFTRNALRHKVLPVVQAQFPAVAPTLARTAALLREGADLLDDLARLDE
NACGGPVLAADVFASLPAARAANLLRWQTARMGARAPSRARLGETLRQLREAPGPLRLPLGDLWCCAYQGRVWLERDDEA
PLRAHLWCGESSLGWGAGQVRLSQGGGGAALRVAAGEAMLAPPAPGLRMRLGPGRPTRSFKNLCQEAGIPPWLRPRLPVL
WVAGEPAWIGGIGIAAQFQCAEGEQGVVPAWVPASAAQQGQHLGQFDR

Sequences:

>Translated_448_residues
MAVELAGPFAEVLRAAAIGPASRLCCALSGGVDSVVTLDLLTRLQPRFGFTLTAVHVHHGLSPHADAWAQFCARLCAARG
LTLAIRRVEVPTDTGQGLESAARARRHAELVALPCDWLVFGHHQDDQAETVLFRLFRGSGLRGLGAMAAVEPGQHAMPGK
LRPLLDIGRAGIVAYARAAGLEWIEDESNADCRFTRNALRHKVLPVVQAQFPAVAPTLARTAALLREGADLLDDLARLDE
NACGGPVLAADVFASLPAARAANLLRWQTARMGARAPSRARLGETLRQLREAPGPLRLPLGDLWCCAYQGRVWLERDDEA
PLRAHLWCGESSLGWGAGQVRLSQGGGGAALRVAAGEAMLAPPAPGLRMRLGPGRPTRSFKNLCQEAGIPPWLRPRLPVL
WVAGEPAWIGGIGIAAQFQCAEGEQGVVPAWVPASAAQQGQHLGQFDR
>Mature_447_residues
AVELAGPFAEVLRAAAIGPASRLCCALSGGVDSVVTLDLLTRLQPRFGFTLTAVHVHHGLSPHADAWAQFCARLCAARGL
TLAIRRVEVPTDTGQGLESAARARRHAELVALPCDWLVFGHHQDDQAETVLFRLFRGSGLRGLGAMAAVEPGQHAMPGKL
RPLLDIGRAGIVAYARAAGLEWIEDESNADCRFTRNALRHKVLPVVQAQFPAVAPTLARTAALLREGADLLDDLARLDEN
ACGGPVLAADVFASLPAARAANLLRWQTARMGARAPSRARLGETLRQLREAPGPLRLPLGDLWCCAYQGRVWLERDDEAP
LRAHLWCGESSLGWGAGQVRLSQGGGGAALRVAAGEAMLAPPAPGLRMRLGPGRPTRSFKNLCQEAGIPPWLRPRLPVLW
VAGEPAWIGGIGIAAQFQCAEGEQGVVPAWVPASAAQQGQHLGQFDR

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=425, Percent_Identity=37.1764705882353, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_AZOSB (A1K3X8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932420.1
- ProteinModelPortal:   A1K3X8
- SMR:   A1K3X8
- STRING:   A1K3X8
- GeneID:   4608254
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0916
- eggNOG:   COG0037
- HOGENOM:   HBG668702
- OMA:   GADSAML
- PhylomeDB:   A1K3X8
- ProtClustDB:   CLSK766773
- BioCyc:   ASP62928:AZO0916-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- SMART:   SM00977
- TIGRFAMs:   TIGR02433
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C; SSF56037 B3_4

EC number: NA

Molecular weight: Translated: 47757; Mature: 47625

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVELAGPFAEVLRAAAIGPASRLCCALSGGVDSVVTLDLLTRLQPRFGFTLTAVHVHHG
CEEECCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
LSPHADAWAQFCARLCAARGLTLAIRRVEVPTDTGQGLESAARARRHAELVALPCDWLVF
CCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCEEEEECCCEEEE
GHHQDDQAETVLFRLFRGSGLRGLGAMAAVEPGQHAMPGKLRPLLDIGRAGIVAYARAAG
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCC
LEWIEDESNADCRFTRNALRHKVLPVVQAQFPAVAPTLARTAALLREGADLLDDLARLDE
CCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCC
NACGGPVLAADVFASLPAARAANLLRWQTARMGARAPSRARLGETLRQLREAPGPLRLPL
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCC
GDLWCCAYQGRVWLERDDEAPLRAHLWCGESSLGWGAGQVRLSQGGGGAALRVAAGEAML
CCEEEEEECCEEEEECCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCEEE
APPAPGLRMRLGPGRPTRSFKNLCQEAGIPPWLRPRLPVLWVAGEPAWIGGIGIAAQFQC
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEECCCEEEEEEE
AEGEQGVVPAWVPASAAQQGQHLGQFDR
CCCCCCCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
AVELAGPFAEVLRAAAIGPASRLCCALSGGVDSVVTLDLLTRLQPRFGFTLTAVHVHHG
EEECCCHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCC
LSPHADAWAQFCARLCAARGLTLAIRRVEVPTDTGQGLESAARARRHAELVALPCDWLVF
CCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHHHHHHHHCCEEEEECCCEEEE
GHHQDDQAETVLFRLFRGSGLRGLGAMAAVEPGQHAMPGKLRPLLDIGRAGIVAYARAAG
CCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCC
LEWIEDESNADCRFTRNALRHKVLPVVQAQFPAVAPTLARTAALLREGADLLDDLARLDE
CCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCC
NACGGPVLAADVFASLPAARAANLLRWQTARMGARAPSRARLGETLRQLREAPGPLRLPL
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEECCC
GDLWCCAYQGRVWLERDDEAPLRAHLWCGESSLGWGAGQVRLSQGGGGAALRVAAGEAML
CCEEEEEECCEEEEECCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCEEEEEECCCEEE
APPAPGLRMRLGPGRPTRSFKNLCQEAGIPPWLRPRLPVLWVAGEPAWIGGIGIAAQFQC
CCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCCEEECCCEEEEEEE
AEGEQGVVPAWVPASAAQQGQHLGQFDR
CCCCCCCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA