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The map label for this gene is purB [H]

Identifier: 119897192

GI number: 119897192

Start: 965057

End: 966433

Strand: Direct

Name: purB [H]

Synonym: azo0901

Alternate gene names: 119897192

Gene position: 965057-966433 (Clockwise)

Preceding gene: 119897188

Following gene: 119897193

Centisome position: 22.05

GC content: 65.14

Gene sequence:

>1377_bases
ATGTCCCTCGCCGCACCCTCCGCCCTCACCGCCCTTTCCCCGCTCGATGGCCGTTACCACGGCAAGGTGGAAGGCCTGCG
CGACCACTTCTCTGAGCATGGCCTGATCCGCAACCGGGTCAAGGTTGAAGTCGAGTGGCTGAAGGCGCTCGCGGCCGACG
GCGCGCTGGCCGAGATCGCACCGTTTTCGGCGGCGACGATCGCCGAACTCGACAGCGTGGTGTCCGCCTTTGCGCCGGCC
GATGGCGAAGCGGTGAAGGCGATCGAAGCCACCACCAACCACGACGTGAAGGCGATGGAGTACTGGCTGAAGAAGCGCCT
CGGCCACAACGCCGAAGTGATGAAAGTGTCGGAATTCATCCATTTCGCCTGCACTTCGGAGGATATCAACAACACCTCCC
ACGCCCTGATGCTGAAGGCCGGGCGCGACGAGGTGCTGCTGCCGGCGGTGGACAAGGTGATCGCCCGCTTCCGCGAACTG
GCGCACCAGCTTGCCGACCTGCCGATGCTGTCGCGCACCCACGGCCAGCCCGCCAGCCCGACCACGCTGGGCAAGGAGAT
GGCCAACATCGCGGCCCGGCTGATCCGCGCCCGCGCGCAGATCGCCAATGTGTCGCTCACTGCCAAGTTCAATGGCGCGG
TCGGCAACTACAATGCCCACCTGTCGGCGTGGCCTGAATTCGACTGGGAAGGCTTCAACAAGCGCTTCATCGAATCGCTC
GGCCTCGAGTTCAACCCCTACACCATCCAGATCGAACCCCATGACGCGATGGCCGAACTGTTCGACGCCATCGGCCGCAT
GAACACCATCCTGATCGACGCCTGCCGCGACATCTGGATGTACATCTCCTTTGGCTACTTCAAGCAGAAGCTGAAGGAGG
GCGAGGTCGGTTCGTCGACCATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACGCCGAAGGCAACCTCGGCATCGCC
AACGCGGTGCTGCGCCACTTCTCGGAGAAGCTGCCGGTGTCGCGCATGCAGCGCGACCTCACCGACTCCACCGTGCTGCG
CAACATGGGCGTGGGCTTCGGCCATACCGTGCTGGCGCTCGATTCCGCGCTGCGCGGCCTGGGCAAGCTGGAGGCCGAAC
CGGCCCGCCTTGCCGCCGACCTGGCCGAGTGCTGGGAAGTGCTGGCCGAGCCGGTGCAGACCGTGATGCGCCGCTTCGGC
ATCGAGAACCCGTACGAACAGCTGAAGGCGATGACGCGCGGCAAGGGCATCACCCGTGAGGCACTGCAGGAGTTCATCGG
TACGCTGGAGATTCCGCAGAGCGAGCGCGACCGCCTGCTGGCGATGACGCCGGCGAGCTACGTCGGCAAGGCCGCGGAGC
TTGCCCGCCGTATTTGA

Upstream 100 bases:

>100_bases
GTACTTGCAAGAAACCTGCCTGACCACAGCGTCTTTTTGTGTTGGCCGCCTCGGGAAAGGGCCGGAGCGGTAAAATAGCA
TTTTGTCCGGAAGTCCTGTC

Downstream 100 bases:

>100_bases
CCGACGCCCGCCGGCCGCCGCGCCGGCATTGCCGCGACGCCACCCGAGGGTGGCGTTTTCCATCCTGAAGGAGCACACAT
GGCTTTTGCCGATACGCTGA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSLAAPSALTALSPLDGRYHGKVEGLRDHFSEHGLIRNRVKVEVEWLKALAADGALAEIAPFSAATIAELDSVVSAFAPA
DGEAVKAIEATTNHDVKAMEYWLKKRLGHNAEVMKVSEFIHFACTSEDINNTSHALMLKAGRDEVLLPAVDKVIARFREL
AHQLADLPMLSRTHGQPASPTTLGKEMANIAARLIRARAQIANVSLTAKFNGAVGNYNAHLSAWPEFDWEGFNKRFIESL
GLEFNPYTIQIEPHDAMAELFDAIGRMNTILIDACRDIWMYISFGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNLGIA
NAVLRHFSEKLPVSRMQRDLTDSTVLRNMGVGFGHTVLALDSALRGLGKLEAEPARLAADLAECWEVLAEPVQTVMRRFG
IENPYEQLKAMTRGKGITREALQEFIGTLEIPQSERDRLLAMTPASYVGKAAELARRI

Sequences:

>Translated_458_residues
MSLAAPSALTALSPLDGRYHGKVEGLRDHFSEHGLIRNRVKVEVEWLKALAADGALAEIAPFSAATIAELDSVVSAFAPA
DGEAVKAIEATTNHDVKAMEYWLKKRLGHNAEVMKVSEFIHFACTSEDINNTSHALMLKAGRDEVLLPAVDKVIARFREL
AHQLADLPMLSRTHGQPASPTTLGKEMANIAARLIRARAQIANVSLTAKFNGAVGNYNAHLSAWPEFDWEGFNKRFIESL
GLEFNPYTIQIEPHDAMAELFDAIGRMNTILIDACRDIWMYISFGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNLGIA
NAVLRHFSEKLPVSRMQRDLTDSTVLRNMGVGFGHTVLALDSALRGLGKLEAEPARLAADLAECWEVLAEPVQTVMRRFG
IENPYEQLKAMTRGKGITREALQEFIGTLEIPQSERDRLLAMTPASYVGKAAELARRI
>Mature_457_residues
SLAAPSALTALSPLDGRYHGKVEGLRDHFSEHGLIRNRVKVEVEWLKALAADGALAEIAPFSAATIAELDSVVSAFAPAD
GEAVKAIEATTNHDVKAMEYWLKKRLGHNAEVMKVSEFIHFACTSEDINNTSHALMLKAGRDEVLLPAVDKVIARFRELA
HQLADLPMLSRTHGQPASPTTLGKEMANIAARLIRARAQIANVSLTAKFNGAVGNYNAHLSAWPEFDWEGFNKRFIESLG
LEFNPYTIQIEPHDAMAELFDAIGRMNTILIDACRDIWMYISFGYFKQKLKEGEVGSSTMPHKVNPIDFENAEGNLGIAN
AVLRHFSEKLPVSRMQRDLTDSTVLRNMGVGFGHTVLALDSALRGLGKLEAEPARLAADLAECWEVLAEPVQTVMRRFGI
ENPYEQLKAMTRGKGITREALQEFIGTLEIPQSERDRLLAMTPASYVGKAAELARRI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI183227688, Length=300, Percent_Identity=24, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI4557269, Length=300, Percent_Identity=24, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1787376, Length=445, Percent_Identity=59.3258426966292, Blast_Score=568, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24647570, Length=307, Percent_Identity=27.3615635179153, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 50500; Mature: 50369

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAAPSALTALSPLDGRYHGKVEGLRDHFSEHGLIRNRVKVEVEWLKALAADGALAEIA
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHC
PFSAATIAELDSVVSAFAPADGEAVKAIEATTNHDVKAMEYWLKKRLGHNAEVMKVSEFI
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
HFACTSEDINNTSHALMLKAGRDEVLLPAVDKVIARFRELAHQLADLPMLSRTHGQPASP
HHHCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
TTLGKEMANIAARLIRARAQIANVSLTAKFNGAVGNYNAHLSAWPEFDWEGFNKRFIESL
HHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GLEFNPYTIQIEPHDAMAELFDAIGRMNTILIDACRDIWMYISFGYFKQKLKEGEVGSST
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MPHKVNPIDFENAEGNLGIANAVLRHFSEKLPVSRMQRDLTDSTVLRNMGVGFGHTVLAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
DSALRGLGKLEAEPARLAADLAECWEVLAEPVQTVMRRFGIENPYEQLKAMTRGKGITRE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
ALQEFIGTLEIPQSERDRLLAMTPASYVGKAAELARRI
HHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLAAPSALTALSPLDGRYHGKVEGLRDHFSEHGLIRNRVKVEVEWLKALAADGALAEIA
CCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHC
PFSAATIAELDSVVSAFAPADGEAVKAIEATTNHDVKAMEYWLKKRLGHNAEVMKVSEFI
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
HFACTSEDINNTSHALMLKAGRDEVLLPAVDKVIARFRELAHQLADLPMLSRTHGQPASP
HHHCCCCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
TTLGKEMANIAARLIRARAQIANVSLTAKFNGAVGNYNAHLSAWPEFDWEGFNKRFIESL
HHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
GLEFNPYTIQIEPHDAMAELFDAIGRMNTILIDACRDIWMYISFGYFKQKLKEGEVGSST
CCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MPHKVNPIDFENAEGNLGIANAVLRHFSEKLPVSRMQRDLTDSTVLRNMGVGFGHTVLAL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHH
DSALRGLGKLEAEPARLAADLAECWEVLAEPVQTVMRRFGIENPYEQLKAMTRGKGITRE
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHH
ALQEFIGTLEIPQSERDRLLAMTPASYVGKAAELARRI
HHHHHHCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]