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The map label for this gene is ddl

Identifier: 119897177

GI number: 119897177

Start: 948497

End: 949411

Strand: Direct

Name: ddl

Synonym: azo0886

Alternate gene names: 119897177

Gene position: 948497-949411 (Clockwise)

Preceding gene: 119897176

Following gene: 119897178

Centisome position: 21.67

GC content: 68.52

Gene sequence:

>915_bases
GTGAAGCAGCGGTTTGGAAAAGTGGCGGTGCTGTTCGGCGGCTCTTCCGCCGAGCGCGATGTTTCGCTGATGTCGGGCGC
GGCGGTGCTGGCCGCGCTGCAGGGTGCCGGGGTCGATGCGCACGCCTTCGACCCGGCCGAGCGCGACCTCCACATCCTCA
AGGAAGAGGGCTACGACCGCGTCTTCATCGCGCTGCACGGCCGCGGCGGCGAAGACGGTACGGTGCAGGGCGCGCTCGAA
CTGATGGGCATCCCCTACACCGGCAGCGGCGTGATGGCCTCGGCGCTGGCGATGGACAAATGGCGGACCAAGATGGTGTG
GCTGTCCTGCGGGTTGCCGACGCCGCGCTACGCCATCCTCGACGCCGACAGCGACTTCGATGCGATCGCGCGCGACCTCG
GCCTGCCGATCTTCGTCAAGCCGGTGCATGAAGGCTCCAGCATGGGCGCGACCAAGGTGACCGAGGCCGGCCAGTTGCGC
GCCGCGTGGGAACTGGCTGCGCGTTACGACAGCCTGGTGATCGCGGAGGAGTTCATCTCCGGCCAGGAGCTGACCGCGCC
TTTCCTCGACGACCGCGCGCTGCCGCTGGTGCGCATCGTCGCCCCCGACGGCAACTACGACTACCAGCACAAGTACTTCA
CCGACGACACCCGCTACGACTGCCCCTGCGGCCTGCCGCAGGCCGAAGAAGAGGCGCTGCAGGCGCTGATCCTCAAGAGC
GCGCGCGTGCTCGGCTGCCGCGGCTGGGGCCGCGCCGACCTGATCCTGACGCCCGAAGGGCGGCCCTATCTGCTGGAGAT
GAATACCTCGCCCGGCATGACCGGCCATTCGCTGGTGCCGATGTCGGCACGTGTGGCGGGCATGTCGTTCGAAGCGCTGT
GTCTGGCGATTCTCGCGGGAGCCCGTCTTGGCTGA

Upstream 100 bases:

>100_bases
CGGTGCTGGAGGCGGTGCGCGACGGCGATGTGGTGATCACGATGGGTGCGGGCAGCATCGGCGCGGTGCCGGGCAAGCTC
GCCAGCAACGAGGAGCAGGC

Downstream 100 bases:

>100_bases
GTTCCGCACGCGCGCCTCCGCCGCTGCTGCCCCGCGTGGGGCGGCGCGCGAGCGCGCGCGCGTGGAGCGCGGCCTGTGGC
ACCGGCCTGCGCTGCTGCAC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MKQRFGKVAVLFGGSSAERDVSLMSGAAVLAALQGAGVDAHAFDPAERDLHILKEEGYDRVFIALHGRGGEDGTVQGALE
LMGIPYTGSGVMASALAMDKWRTKMVWLSCGLPTPRYAILDADSDFDAIARDLGLPIFVKPVHEGSSMGATKVTEAGQLR
AAWELAARYDSLVIAEEFISGQELTAPFLDDRALPLVRIVAPDGNYDYQHKYFTDDTRYDCPCGLPQAEEEALQALILKS
ARVLGCRGWGRADLILTPEGRPYLLEMNTSPGMTGHSLVPMSARVAGMSFEALCLAILAGARLG

Sequences:

>Translated_304_residues
MKQRFGKVAVLFGGSSAERDVSLMSGAAVLAALQGAGVDAHAFDPAERDLHILKEEGYDRVFIALHGRGGEDGTVQGALE
LMGIPYTGSGVMASALAMDKWRTKMVWLSCGLPTPRYAILDADSDFDAIARDLGLPIFVKPVHEGSSMGATKVTEAGQLR
AAWELAARYDSLVIAEEFISGQELTAPFLDDRALPLVRIVAPDGNYDYQHKYFTDDTRYDCPCGLPQAEEEALQALILKS
ARVLGCRGWGRADLILTPEGRPYLLEMNTSPGMTGHSLVPMSARVAGMSFEALCLAILAGARLG
>Mature_304_residues
MKQRFGKVAVLFGGSSAERDVSLMSGAAVLAALQGAGVDAHAFDPAERDLHILKEEGYDRVFIALHGRGGEDGTVQGALE
LMGIPYTGSGVMASALAMDKWRTKMVWLSCGLPTPRYAILDADSDFDAIARDLGLPIFVKPVHEGSSMGATKVTEAGQLR
AAWELAARYDSLVIAEEFISGQELTAPFLDDRALPLVRIVAPDGNYDYQHKYFTDDTRYDCPCGLPQAEEEALQALILKS
ARVLGCRGWGRADLILTPEGRPYLLEMNTSPGMTGHSLVPMSARVAGMSFEALCLAILAGARLG

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=300, Percent_Identity=54, Blast_Score=313, Evalue=8e-87,
Organism=Escherichia coli, GI1786579, Length=343, Percent_Identity=30.6122448979592, Blast_Score=145, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_AZOSB (A1K3U8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932390.1
- ProteinModelPortal:   A1K3U8
- SMR:   A1K3U8
- STRING:   A1K3U8
- GeneID:   4609289
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0886
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   A1K3U8
- ProtClustDB:   PRK01372
- BioCyc:   ASP62928:AZO0886-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32636; Mature: 32636

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRFGKVAVLFGGSSAERDVSLMSGAAVLAALQGAGVDAHAFDPAERDLHILKEEGYDR
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCE
VFIALHGRGGEDGTVQGALELMGIPYTGSGVMASALAMDKWRTKMVWLSCGLPTPRYAIL
EEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCEEEE
DADSDFDAIARDLGLPIFVKPVHEGSSMGATKVTEAGQLRAAWELAARYDSLVIAEEFIS
ECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEHHHCC
GQELTAPFLDDRALPLVRIVAPDGNYDYQHKYFTDDTRYDCPCGLPQAEEEALQALILKS
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
ARVLGCRGWGRADLILTPEGRPYLLEMNTSPGMTGHSLVPMSARVAGMSFEALCLAILAG
HHEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCEECCCHHHCCCCCHHHHHHHHHHC
ARLG
CCCC
>Mature Secondary Structure
MKQRFGKVAVLFGGSSAERDVSLMSGAAVLAALQGAGVDAHAFDPAERDLHILKEEGYDR
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCE
VFIALHGRGGEDGTVQGALELMGIPYTGSGVMASALAMDKWRTKMVWLSCGLPTPRYAIL
EEEEEECCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCEEEE
DADSDFDAIARDLGLPIFVKPVHEGSSMGATKVTEAGQLRAAWELAARYDSLVIAEEFIS
ECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEHHHCC
GQELTAPFLDDRALPLVRIVAPDGNYDYQHKYFTDDTRYDCPCGLPQAEEEALQALILKS
CCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
ARVLGCRGWGRADLILTPEGRPYLLEMNTSPGMTGHSLVPMSARVAGMSFEALCLAILAG
HHEEEECCCCCEEEEECCCCCEEEEEECCCCCCCCCEECCCHHHCCCCCHHHHHHHHHHC
ARLG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA