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The map label for this gene is mraW

Identifier: 119897167

GI number: 119897167

Start: 936433

End: 937371

Strand: Direct

Name: mraW

Synonym: azo0876

Alternate gene names: 119897167

Gene position: 936433-937371 (Clockwise)

Preceding gene: 119897166

Following gene: 119897168

Centisome position: 21.4

GC content: 68.58

Gene sequence:

>939_bases
GTGAGCGCCCAACTGCAACACGTCAGCGTGCTGCTCACCGAGGCGGTGGATGCGCTGGCGATCAAGCCGGAAGGCATTTA
CGTGGACGGCACCTTCGGCCGCGGCGGCCACAGCCGCGCCGTGCTCGCCCGGCTGGGCGCGGGCGGCAGGCTCATCGCTT
TCGATCGCGACCCCGCGGCGATCGCGGCGGGTCAGGCGATCGCCGATCCGCGCCTCGAACTCGTCCATGCCCCTTTCAGC
GAATTCGCGGTCGTGCTCGACCGTTTGGGCGTCAGCGCCGTCGACGGCGTGCTGCTCGACCTTGGCGTGTCGTCGCCGCA
GCTCGACGATGCCAGCCGCGGCATGAGCTTTCGCTTCGACGCGCCGCTGGACATGCGCATGGACACAAGCCGCGGACGCA
CCGTCGCGGAATGGCTGGCGGAAGCCTCCGTCGCCGAGATCACGGAGGTCATCAGAGACTATGGGGAAGAACGGTTTGCT
TATGCGATTGCAAAGGCGATTGCAGCTGCTCGGACAGGGGGGGCTATCGCAACCACTGGACAGCTTGCCGCGATCGTGGA
AAAGGCGGTCCGTACACGCGAGCCGGGGCAGCACCCAGCGACGCGCAGCTTCCAGGCTCTACGGATTTTCATCAATCAGG
AACTCGAAGAGCTGACGACCGTGCTGCCGGACTGCGTTGCGCGCCTGAGCGAGGGCGGCCGGCTGGTGGTGATCAGCTTT
CATTCGCTCGAGGACCGCATCGTCAAGCGCTTCCTGCGCGACGAAGCGCGTCCGCCGCAGCTGCCGTCGCGGCTGCCGGT
GCGCGCGGCCGACCTCCCGCCGCCCCGGCTGCAACTCGTCGGCAAGGCGCAGCGGCCGGGCGAGGCTGAAGTGGCGGCCA
ATCCCCGCGCGCGTAGCGCAGTGATGCGGGTGGCCGAGCGCTGCGGGGTGGCTGCATGA

Upstream 100 bases:

>100_bases
ACTTCGAACTCTGGTCGGATGCCGGCTGGCAGAAGCAGCAGGAGGCGATGCTGGCGCTGGTCGACAACCCCTTGCCCGCC
GGACTGGAGAACTTCGTCCT

Downstream 100 bases:

>100_bases
TCCGCTTCGACGCAATGCTCGTCGCACTCGTGGTCGCCAGCGCGCTCGGCGTGGTGGCCGCGCAGCACCAGTCGCGCAAG
CTCTACATCGAGCTGGAGCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSAQLQHVSVLLTEAVDALAIKPEGIYVDGTFGRGGHSRAVLARLGAGGRLIAFDRDPAAIAAGQAIADPRLELVHAPFS
EFAVVLDRLGVSAVDGVLLDLGVSSPQLDDASRGMSFRFDAPLDMRMDTSRGRTVAEWLAEASVAEITEVIRDYGEERFA
YAIAKAIAAARTGGAIATTGQLAAIVEKAVRTREPGQHPATRSFQALRIFINQELEELTTVLPDCVARLSEGGRLVVISF
HSLEDRIVKRFLRDEARPPQLPSRLPVRAADLPPPRLQLVGKAQRPGEAEVAANPRARSAVMRVAERCGVAA

Sequences:

>Translated_312_residues
MSAQLQHVSVLLTEAVDALAIKPEGIYVDGTFGRGGHSRAVLARLGAGGRLIAFDRDPAAIAAGQAIADPRLELVHAPFS
EFAVVLDRLGVSAVDGVLLDLGVSSPQLDDASRGMSFRFDAPLDMRMDTSRGRTVAEWLAEASVAEITEVIRDYGEERFA
YAIAKAIAAARTGGAIATTGQLAAIVEKAVRTREPGQHPATRSFQALRIFINQELEELTTVLPDCVARLSEGGRLVVISF
HSLEDRIVKRFLRDEARPPQLPSRLPVRAADLPPPRLQLVGKAQRPGEAEVAANPRARSAVMRVAERCGVAA
>Mature_311_residues
SAQLQHVSVLLTEAVDALAIKPEGIYVDGTFGRGGHSRAVLARLGAGGRLIAFDRDPAAIAAGQAIADPRLELVHAPFSE
FAVVLDRLGVSAVDGVLLDLGVSSPQLDDASRGMSFRFDAPLDMRMDTSRGRTVAEWLAEASVAEITEVIRDYGEERFAY
AIAKAIAAARTGGAIATTGQLAAIVEKAVRTREPGQHPATRSFQALRIFINQELEELTTVLPDCVARLSEGGRLVVISFH
SLEDRIVKRFLRDEARPPQLPSRLPVRAADLPPPRLQLVGKAQRPGEAEVAANPRARSAVMRVAERCGVAA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=39.1691394658754, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI165377202, Length=197, Percent_Identity=39.0862944162437, Blast_Score=118, Evalue=8e-27,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=53.0351437699681, Blast_Score=327, Evalue=8e-91,
Organism=Drosophila melanogaster, GI62472493, Length=333, Percent_Identity=38.1381381381381, Blast_Score=171, Evalue=4e-43,
Organism=Drosophila melanogaster, GI28571637, Length=333, Percent_Identity=38.1381381381381, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_AZOSB (A1K3T8)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_932380.1
- ProteinModelPortal:   A1K3T8
- SMR:   A1K3T8
- STRING:   A1K3T8
- GeneID:   4609270
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0876
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- PhylomeDB:   A1K3T8
- ProtClustDB:   PRK00050
- BioCyc:   ASP62928:AZO0876-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 33423; Mature: 33291

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQLQHVSVLLTEAVDALAIKPEGIYVDGTFGRGGHSRAVLARLGAGGRLIAFDRDPAA
CCCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCHH
IAAGQAIADPRLELVHAPFSEFAVVLDRLGVSAVDGVLLDLGVSSPQLDDASRGMSFRFD
HHCCCHHCCCCHHEEECCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHCCCEEEEC
APLDMRMDTSRGRTVAEWLAEASVAEITEVIRDYGEERFAYAIAKAIAAARTGGAIATTG
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEHH
QLAAIVEKAVRTREPGQHPATRSFQALRIFINQELEELTTVLPDCVARLSEGGRLVVISF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
HSLEDRIVKRFLRDEARPPQLPSRLPVRAADLPPPRLQLVGKAQRPGEAEVAANPRARSA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCHHHHH
VMRVAERCGVAA
HHHHHHHCCCCC
>Mature Secondary Structure 
SAQLQHVSVLLTEAVDALAIKPEGIYVDGTFGRGGHSRAVLARLGAGGRLIAFDRDPAA
CCCHHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCHH
IAAGQAIADPRLELVHAPFSEFAVVLDRLGVSAVDGVLLDLGVSSPQLDDASRGMSFRFD
HHCCCHHCCCCHHEEECCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCHHHCCCEEEEC
APLDMRMDTSRGRTVAEWLAEASVAEITEVIRDYGEERFAYAIAKAIAAARTGGAIATTG
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEHH
QLAAIVEKAVRTREPGQHPATRSFQALRIFINQELEELTTVLPDCVARLSEGGRLVVISF
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
HSLEDRIVKRFLRDEARPPQLPSRLPVRAADLPPPRLQLVGKAQRPGEAEVAANPRARSA
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHEECCCCCCCCCCCCCCHHHHH
VMRVAERCGVAA
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA