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The map label for this gene is ahpF [H]

Identifier: 119897061

GI number: 119897061

Start: 821802

End: 823355

Strand: Direct

Name: ahpF [H]

Synonym: azo0770

Alternate gene names: 119897061

Gene position: 821802-823355 (Clockwise)

Preceding gene: 119897060

Following gene: 119897068

Centisome position: 18.78

GC content: 64.61

Gene sequence:

>1554_bases
ATGCTGGACGCCAACATCAAGACTCAACTGAAAGCCTATCTGGAGCGGGTGACGCAGCCGATCGAGATCGTGGCGTCGCT
GGACGATGGCGCGAAGTCGCGCGAAATGCAGGAACTGCTGGCCGACGTCGCCGAGCAGTCCCACCTGATCACCGTGATCG
AGCGGCGCGATGACAATGAGCGCAAGCCCTCGTTCTCGGTCGGCCCCAAGGGCGGAGAGGCGCGCGTGCGCTTTGCCGGC
CTGCCGATGGGCCACGAATTCACCTCGCTCATCCTCGCGCTGCTGCAGTCGGCGGGTTATCCGCCGAAGGTCGAAGCCGA
CGTGATCGAGCAGATCAAGGCGCTCGAAGGCGAGTTCAACTTCGAGACCTACATCTCGCTGTCCTGCCACAACTGCCCGG
ACGTGGTGCAGGCCTTGAACCTGCTGTCCATCCTCAACCCCAACATCCGCCACACCATGATCGACGGCGGCGTGTTCCAG
GAAGAGGTCGAGAAGCGCCAGATCATGGCGGTGCCCACCGTCTATCTAAACGGCCAGGAGTTTGGCGCCGGCCGCATGGA
ACTCGGTGAAATCCTCGCCAAGATCGACACCGGTGCGGCCAAGCGCGACGCGGCCAAGCTCTCCGCCAAGGACGTGTTCG
ACGTGCTCGTCGTCGGCGGCGGTCCGGCGGGCGCCGCGGCGGCGATCTACGCGGCGCGCAAGGGCATCCGCACCGGCGTG
CTGGCCGAACGCTTCGGCGGCCAGGTGATGGACACGCTCGCGATCGAGAACTTCATCTCGGTGAAGGAAACCGAAGGTCC
GAAGCTCGCGATGGCGCTGGAGGAGCACGTCAAGCAGTACGAGGTCGACGTGATGAACCTGCAGCGGGCGACCGCACTGG
TGCCGGGCGACATCCACGAGGTCAGGCTTGAAAACGGTGGCACGCTGAAGGCGAAGACGGTGATTCTGGCGACCGGGGCG
CGCTGGCGCGAAATGAACGTGCCGGGCGAAAAGGAATACCGCGGCAAGGGCGTGGCTTACTGCCCGCACTGCGACGGCCC
GCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGCGGCAACTCCGGCGTTGAAGCCGCGATCGATCTCGCCGGCATCG
TCGCCCATGTCACGCTGCTGGAATTTGCCGACACCCTGCGTGCGGACGCGGTGCTGCAGAAGAAGCTCGCCAGCCTGCCC
AACGTCACGGTGATCAAGAGCGCACAGACCACCGAGGTGACCGGGGCCGAAAAGGTGAATGGCCTGGTGTACAAGGACCG
CGTGAGCGGCGAAGAAAAGCGCCTGGAGTTGGAAGGCATCTTCGTGCAGATCGGTCTGCTGCCCAACACCGACTTCCTGA
AGGGCACGGTCGAACTCACCCGCTTCGGGGAGATCATCGTCGATGCCAAGGGTCAGACCTCGGTGCCGGGCGTATTCGCC
GCGGGCGACTGCACCACGGTGCCGTACAAGCAGATCATCATCGCGATGGGTGAGGGATCAAAGGCCTCGCTGGCGGCCTT
CGATCACCTGATCCGGACGTCGGTGCCTGCCTGA

Upstream 100 bases:

>100_bases
CGGCCCGGGGCGATGTGCTCTGCAGCCGGCCATGCGCCGGCTGTGTCGTATCTGGCCGCCGAAAACACCGGCGGCGCCGA
ACAAGCAAGGAAAAATCATC

Downstream 100 bases:

>100_bases
GCCTTCCGGTCGCCATGTACGGAAATCCCGCCCGGTGGCGGGATTTCCGCTTCCAACGGGTCGATATGACTTTCGTGTGA
TCGAGGCGGGAAATGAAAAG

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MLDANIKTQLKAYLERVTQPIEIVASLDDGAKSREMQELLADVAEQSHLITVIERRDDNERKPSFSVGPKGGEARVRFAG
LPMGHEFTSLILALLQSAGYPPKVEADVIEQIKALEGEFNFETYISLSCHNCPDVVQALNLLSILNPNIRHTMIDGGVFQ
EEVEKRQIMAVPTVYLNGQEFGAGRMELGEILAKIDTGAAKRDAAKLSAKDVFDVLVVGGGPAGAAAAIYAARKGIRTGV
LAERFGGQVMDTLAIENFISVKETEGPKLAMALEEHVKQYEVDVMNLQRATALVPGDIHEVRLENGGTLKAKTVILATGA
RWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFADTLRADAVLQKKLASLP
NVTVIKSAQTTEVTGAEKVNGLVYKDRVSGEEKRLELEGIFVQIGLLPNTDFLKGTVELTRFGEIIVDAKGQTSVPGVFA
AGDCTTVPYKQIIIAMGEGSKASLAAFDHLIRTSVPA

Sequences:

>Translated_517_residues
MLDANIKTQLKAYLERVTQPIEIVASLDDGAKSREMQELLADVAEQSHLITVIERRDDNERKPSFSVGPKGGEARVRFAG
LPMGHEFTSLILALLQSAGYPPKVEADVIEQIKALEGEFNFETYISLSCHNCPDVVQALNLLSILNPNIRHTMIDGGVFQ
EEVEKRQIMAVPTVYLNGQEFGAGRMELGEILAKIDTGAAKRDAAKLSAKDVFDVLVVGGGPAGAAAAIYAARKGIRTGV
LAERFGGQVMDTLAIENFISVKETEGPKLAMALEEHVKQYEVDVMNLQRATALVPGDIHEVRLENGGTLKAKTVILATGA
RWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFADTLRADAVLQKKLASLP
NVTVIKSAQTTEVTGAEKVNGLVYKDRVSGEEKRLELEGIFVQIGLLPNTDFLKGTVELTRFGEIIVDAKGQTSVPGVFA
AGDCTTVPYKQIIIAMGEGSKASLAAFDHLIRTSVPA
>Mature_517_residues
MLDANIKTQLKAYLERVTQPIEIVASLDDGAKSREMQELLADVAEQSHLITVIERRDDNERKPSFSVGPKGGEARVRFAG
LPMGHEFTSLILALLQSAGYPPKVEADVIEQIKALEGEFNFETYISLSCHNCPDVVQALNLLSILNPNIRHTMIDGGVFQ
EEVEKRQIMAVPTVYLNGQEFGAGRMELGEILAKIDTGAAKRDAAKLSAKDVFDVLVVGGGPAGAAAAIYAARKGIRTGV
LAERFGGQVMDTLAIENFISVKETEGPKLAMALEEHVKQYEVDVMNLQRATALVPGDIHEVRLENGGTLKAKTVILATGA
RWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLLEFADTLRADAVLQKKLASLP
NVTVIKSAQTTEVTGAEKVNGLVYKDRVSGEEKRLELEGIFVQIGLLPNTDFLKGTVELTRFGEIIVDAKGQTSVPGVFA
AGDCTTVPYKQIIIAMGEGSKASLAAFDHLIRTSVPA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=65.0579150579151, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=316, Percent_Identity=35.126582278481, Blast_Score=158, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6321898, Length=312, Percent_Identity=34.2948717948718, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6320560, Length=310, Percent_Identity=33.5483870967742, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55556; Mature: 55556

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANIKTQLKAYLERVTQPIEIVASLDDGAKSREMQELLADVAEQSHLITVIERRDDNE
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
RKPSFSVGPKGGEARVRFAGLPMGHEFTSLILALLQSAGYPPKVEADVIEQIKALEGEFN
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
FETYISLSCHNCPDVVQALNLLSILNPNIRHTMIDGGVFQEEVEKRQIMAVPTVYLNGQE
EEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHEEEECEEEECCCC
FGAGRMELGEILAKIDTGAAKRDAAKLSAKDVFDVLVVGGGPAGAAAAIYAARKGIRTGV
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCCHHHH
LAERFGGQVMDTLAIENFISVKETEGPKLAMALEEHVKQYEVDVMNLQRATALVPGDIHE
HHHHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHEECCCCCEE
VRLENGGTLKAKTVILATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGG
EEECCCCEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGIVAHVTLLEFADTLRADAVLQKKLASLPNVTVIKSAQTTEVTGAEKVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHC
GLVYKDRVSGEEKRLELEGIFVQIGLLPNTDFLKGTVELTRFGEIIVDAKGQTSVPGVFA
CEEEECCCCCHHHEEEEEEEEEEEEECCCCCHHHHHHHHEECCEEEEECCCCCCCCCEEE
AGDCTTVPYKQIIIAMGEGSKASLAAFDHLIRTSVPA
CCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLDANIKTQLKAYLERVTQPIEIVASLDDGAKSREMQELLADVAEQSHLITVIERRDDNE
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCC
RKPSFSVGPKGGEARVRFAGLPMGHEFTSLILALLQSAGYPPKVEADVIEQIKALEGEFN
CCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCC
FETYISLSCHNCPDVVQALNLLSILNPNIRHTMIDGGVFQEEVEKRQIMAVPTVYLNGQE
EEEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHEEEECEEEECCCC
FGAGRMELGEILAKIDTGAAKRDAAKLSAKDVFDVLVVGGGPAGAAAAIYAARKGIRTGV
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCCHHHH
LAERFGGQVMDTLAIENFISVKETEGPKLAMALEEHVKQYEVDVMNLQRATALVPGDIHE
HHHHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHEECCCCCEE
VRLENGGTLKAKTVILATGARWREMNVPGEKEYRGKGVAYCPHCDGPLFKGKRVAVIGGG
EEECCCCEEEEEEEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEECC
NSGVEAAIDLAGIVAHVTLLEFADTLRADAVLQKKLASLPNVTVIKSAQTTEVTGAEKVN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHC
GLVYKDRVSGEEKRLELEGIFVQIGLLPNTDFLKGTVELTRFGEIIVDAKGQTSVPGVFA
CEEEECCCCCHHHEEEEEEEEEEEEECCCCCHHHHHHHHEECCEEEEECCCCCCCCCEEE
AGDCTTVPYKQIIIAMGEGSKASLAAFDHLIRTSVPA
CCCCCCCCHHHEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]