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The map label for this gene is glnA [H]

Identifier: 119897029

GI number: 119897029

Start: 788464

End: 789873

Strand: Reverse

Name: glnA [H]

Synonym: azo0738

Alternate gene names: 119897029

Gene position: 789873-788464 (Counterclockwise)

Preceding gene: 119897030

Following gene: 119897028

Centisome position: 18.05

GC content: 63.4

Gene sequence:

>1410_bases
ATGAACGCTCAAGACGTCATGAAGATGATCCAGGAAAACGAAGTGCGCTTCGTGGACCTGCGCTTTACGGATACCCGCGG
CAAGGAACAGCACGTCGGCCTGCCGGTGTCGGCCTTCGAAGAAGATCACTTCGAGCACGGCCACCCGTTCGACGGCTCCT
CGATCGCCGGCTGGAAGGGCATCCAGGCTTCCGACATGATCCTGATGCCGGATGCGGCTTCCGCCTACATCGATCCCTTC
TTCGACGAAACCACCCTGGTGCTGGCTTGCGACGTCATCGAGCCGTCCGACGGCAAGGGCTACGACCGCGACCCGCGCTC
CATCGCCAAGCGCGCCGAGGCCTACCTGAAGAGCACCGGCATCGGCGACACCGCCTTCTTCGGCCCGGAACCCGAGTTCT
TCATCTTCGACGCGGTCGAATGGTCGGTCGACATGTCGGGCGTCTATAGCAAGATCATCTCCGAAGAAGCGGCCTGGTCC
ACCGCCGACAAGTTCGAAGGCGGCAACACCGGCCACCGTCCGAAGGTCAAGGGCGGCTACTTCCCGGTTCCCCCGGTCGA
CAGCCTGAACGACATCCGTGCCGCCATGGTGCTGGCACTCGAAGCCGCCGGCATCCCGGTCGAAGTTCACCACCACGAAG
TGGCGAACGCCGGCCAGTGCGAAATCGGCACCAAGTTCAGCACGCTGACCAAGCGCGCCGACTGGACGCAGACCCTGAAG
TACATCGTCCACAACGTGGCCCACCAGTACGGCAAGACCGCCACCTTCATGCCGAAGCCGATCGTCGGCGACAACGGCTC
GGGCATGCACGTGCACCAGTCGATCTGGAAGGACGGCCAGAACCTGTTCGCGGGCAACGGCTATGCCGGCCTGTCGGAAA
CCGCGCTGTACTACATCGGCGGCATCATCAAGCACGCCCGCGCGCTGAACGCCATCACCAACCCGGCCACCAACTCCTAC
AAGCGTCTGGTGCCGCACTACGAAGCGCCGACCAAGCTGGCCTACTCCGCCCGCAACCGTTCCGCCTCGATCCGCATCCC
GTACGTGGCCAACCCGAAGGGTCGCCGCATCGAGTCGCGCTTCCCGGATCCGGCTGCCAACCCGTACCTGTGCTTCGCCG
CGCTGCTGATGGCCGGCCTGGATGGCATCCAGAACAAGATCCACCCGGGCGACCCGGCCGACAAGAACCTGTACGACCTG
CCGCCGGAAGAAGACGCGCTGATCCCGACCGTGTGTACCAGCCTCGACCAGGCGCTGGAATACCTGGACAAGGATCGCGA
GTTCCTGACCCGTGGCGGCGTGTTCTCCAATGACTTCATCGACGCTTACATCGAGCTGAAGATGGAAGAAGTGAACCGCC
TGCGCGTCACCACCCACCCGGTGGAATTCGACCTGTACTACAGCCTGTAA

Upstream 100 bases:

>100_bases
GCATCGGCCGCGAACCGCCTGGCACAAAAAATGCTTTAAGTCGCCGCAACGGAAGTTCGACCGTTTATTTCAGGGATTGC
CCTTCTCACCAGGAGTGAAT

Downstream 100 bases:

>100_bases
TCGGCTGCGCTACGGCGTCCAATGAAAAAGGACGGGCATTGCCCGTCCTTTTTTTATCGGGTCTAATCGCTCCGCTGTCC
CGTCCCCACCGCAGCTTTTC

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MNAQDVMKMIQENEVRFVDLRFTDTRGKEQHVGLPVSAFEEDHFEHGHPFDGSSIAGWKGIQASDMILMPDAASAYIDPF
FDETTLVLACDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGIGDTAFFGPEPEFFIFDAVEWSVDMSGVYSKIISEEAAWS
TADKFEGGNTGHRPKVKGGYFPVPPVDSLNDIRAAMVLALEAAGIPVEVHHHEVANAGQCEIGTKFSTLTKRADWTQTLK
YIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALYYIGGIIKHARALNAITNPATNSY
KRLVPHYEAPTKLAYSARNRSASIRIPYVANPKGRRIESRFPDPAANPYLCFAALLMAGLDGIQNKIHPGDPADKNLYDL
PPEEDALIPTVCTSLDQALEYLDKDREFLTRGGVFSNDFIDAYIELKMEEVNRLRVTTHPVEFDLYYSL

Sequences:

>Translated_469_residues
MNAQDVMKMIQENEVRFVDLRFTDTRGKEQHVGLPVSAFEEDHFEHGHPFDGSSIAGWKGIQASDMILMPDAASAYIDPF
FDETTLVLACDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGIGDTAFFGPEPEFFIFDAVEWSVDMSGVYSKIISEEAAWS
TADKFEGGNTGHRPKVKGGYFPVPPVDSLNDIRAAMVLALEAAGIPVEVHHHEVANAGQCEIGTKFSTLTKRADWTQTLK
YIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALYYIGGIIKHARALNAITNPATNSY
KRLVPHYEAPTKLAYSARNRSASIRIPYVANPKGRRIESRFPDPAANPYLCFAALLMAGLDGIQNKIHPGDPADKNLYDL
PPEEDALIPTVCTSLDQALEYLDKDREFLTRGGVFSNDFIDAYIELKMEEVNRLRVTTHPVEFDLYYSL
>Mature_469_residues
MNAQDVMKMIQENEVRFVDLRFTDTRGKEQHVGLPVSAFEEDHFEHGHPFDGSSIAGWKGIQASDMILMPDAASAYIDPF
FDETTLVLACDVIEPSDGKGYDRDPRSIAKRAEAYLKSTGIGDTAFFGPEPEFFIFDAVEWSVDMSGVYSKIISEEAAWS
TADKFEGGNTGHRPKVKGGYFPVPPVDSLNDIRAAMVLALEAAGIPVEVHHHEVANAGQCEIGTKFSTLTKRADWTQTLK
YIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALYYIGGIIKHARALNAITNPATNSY
KRLVPHYEAPTKLAYSARNRSASIRIPYVANPKGRRIESRFPDPAANPYLCFAALLMAGLDGIQNKIHPGDPADKNLYDL
PPEEDALIPTVCTSLDQALEYLDKDREFLTRGGVFSNDFIDAYIELKMEEVNRLRVTTHPVEFDLYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=488, Percent_Identity=25.4098360655738, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1790301, Length=469, Percent_Identity=66.9509594882729, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=395, Percent_Identity=26.5822784810127, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51948; Mature: 51948

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQDVMKMIQENEVRFVDLRFTDTRGKEQHVGLPVSAFEEDHFEHGHPFDGSSIAGWKG
CCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
IQASDMILMPDAASAYIDPFFDETTLVLACDVIEPSDGKGYDRDPRSIAKRAEAYLKSTG
CCCCCEEEECCCHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IGDTAFFGPEPEFFIFDAVEWSVDMSGVYSKIISEEAAWSTADKFEGGNTGHRPKVKGGY
CCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCC
FPVPPVDSLNDIRAAMVLALEAAGIPVEVHHHEVANAGQCEIGTKFSTLTKRADWTQTLK
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
YIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHH
GIIKHARALNAITNPATNSYKRLVPHYEAPTKLAYSARNRSASIRIPYVANPKGRRIESR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCC
FPDPAANPYLCFAALLMAGLDGIQNKIHPGDPADKNLYDLPPEEDALIPTVCTSLDQALE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YLDKDREFLTRGGVFSNDFIDAYIELKMEEVNRLRVTTHPVEFDLYYSL
HHHHHHHHHHHCCCCCCHHHHHHHHEEHHHCCEEEEEECCEEEEEEEEC
>Mature Secondary Structure
MNAQDVMKMIQENEVRFVDLRFTDTRGKEQHVGLPVSAFEEDHFEHGHPFDGSSIAGWKG
CCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
IQASDMILMPDAASAYIDPFFDETTLVLACDVIEPSDGKGYDRDPRSIAKRAEAYLKSTG
CCCCCEEEECCCHHHHHCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCC
IGDTAFFGPEPEFFIFDAVEWSVDMSGVYSKIISEEAAWSTADKFEGGNTGHRPKVKGGY
CCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCC
FPVPPVDSLNDIRAAMVLALEAAGIPVEVHHHEVANAGQCEIGTKFSTLTKRADWTQTLK
CCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHH
YIVHNVAHQYGKTATFMPKPIVGDNGSGMHVHQSIWKDGQNLFAGNGYAGLSETALYYIG
HHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHH
GIIKHARALNAITNPATNSYKRLVPHYEAPTKLAYSARNRSASIRIPYVANPKGRRIESR
HHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCC
FPDPAANPYLCFAALLMAGLDGIQNKIHPGDPADKNLYDLPPEEDALIPTVCTSLDQALE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
YLDKDREFLTRGGVFSNDFIDAYIELKMEEVNRLRVTTHPVEFDLYYSL
HHHHHHHHHHHCCCCCCHHHHHHHHEEHHHCCEEEEEECCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]