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The map label for this gene is dnaB [H]

Identifier: 119897013

GI number: 119897013

Start: 768968

End: 770377

Strand: Direct

Name: dnaB [H]

Synonym: azo0722

Alternate gene names: 119897013

Gene position: 768968-770377 (Clockwise)

Preceding gene: 119897012

Following gene: 119897014

Centisome position: 17.57

GC content: 61.56

Gene sequence:

>1410_bases
ATGAGTAGTACTTCCCGCCGGTTTCAGGATGGACCGCATGATCCCGAAATGGCCTCGATCAAGCTTCCTCCGCATTCGCT
GGAGGCCGAGCAGTCACTGATCGGTGGCATCCTGATCGACAACGCGGCCTGGGAGCGCATCGCCGACCTGGTGAACGAGG
CGGACTTCTACCGCGACGATCATCGCCGCATCTACCGCCATATCACCCGCCTCATTGACCTCGGCAAGCCGGCCGACGTC
GTCACTGTTTTCGAATCGCTCGAGAAGAACGGCGAAGCGGAGCAGGCTGGCGGACTGGTGTATCTGGCAGAGATCGCCAA
CAACACGCCGTCGGCGGCGAACATCCGGCGCTACGCCGAAATCGTTCGGGAGCGCGCGATCCTGCGTAAGCTGGTCGCGG
TGGGCGACGAGATCGCGGCGTCCGCGCTGACGCCCTCGGGCAAGGACGCCAAGTCGCTGCTCGATGAGGCGGAGGCGAAG
GTATTCGAGATCGCAGAGGCGGGGGCCCGTCACGCAAGCGGTTTCATTTCGATCCAGCCCATCCTGAAGCAGGTGGTCGA
CCGCGTGCAGGAGCTGTACGACCGCGACAGCCCCTCGGAGGTAACGGGTATTCCGTCCGGTTTCGTCGATCTCGACGACA
AGACGTCCGGCCTGCAGCCGTCGGATATGTTGATCGTCGCCGGGCGGCCGGCCATGGGCAAGACGACGTTTGCGCTTAAC
GTGGCTGAACACGTCGCGGTGGAAGCCCGTTTGCCGGTAGCGATCTTCTCGATGGAAATGCCCGGCACCCAGCTTGCGAC
CCGCTTCATCTCGTCGGTCGGCCGTATCGACATGCAGAAGATCCGCAGCGGGCGGCTCACCGACGAGGACTGGCAGCGCC
TCACCGTTGCAATGGGAAAGCTGTACGACGCGCCGCTCTATATTGATGAAACGCCCGGCCTGAATCCGATTGACCTGCGC
GCACGTGCGCGCCGGCTTGCCCGACAGTGCGGCAAGCTGGGGCTGATCGTGATCGACTACCTGCAGCTGATGACGGGGAG
CAAGGATAGCGATAACCGTGCCGCCGAGCTTTCCGAGATTTCCCGCTCGATCAAGGCGCTGGCAAAAGAGCTGCACGTTC
CTATCATTGCCCTGTCGCAGCTGAATCGAAGCCTGGAACAACGGCCGAACAAACGCCCCGTGATGTCCGACTTGCGTGAG
TCGGGTGCAATTGAGCAGGACGCGGACATCATCATGTTCATTTACCGGGATGAGGTTTACAACCCTGATTCGCCGGACAA
GGGAACTGCAGAGCTGATTATCGGCAAGCACCGTAATGGCCCGACGGGTGTGATTCGCATGACCTTCCTGGGCCAATACA
CGCGCTTCGAGAATTTCGCCGGCGGCGGCTTCTACACAGCGGATGAGTAA

Upstream 100 bases:

>100_bases
GCCCTTTTGCTTTTTGAATCGCCTTTCTTCACAAGGAGCGGTGCGCATCCCTCGCTAGAATGTTGCGTTCCGCCGTCTCC
TTTTCCGTGAGCAGCACGAC

Downstream 100 bases:

>100_bases
TCGCCAAGTTTCTCCGCAATCCGCTTGACCTTTCGAACAAGGGCTTTATACTGCGCGGCTCTTTCGCGTTAGCCCTTGTT
TAGGGCGGTCGCGGAAAAGG

Product: DnaB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSSTSRRFQDGPHDPEMASIKLPPHSLEAEQSLIGGILIDNAAWERIADLVNEADFYRDDHRRIYRHITRLIDLGKPADV
VTVFESLEKNGEAEQAGGLVYLAEIANNTPSAANIRRYAEIVRERAILRKLVAVGDEIAASALTPSGKDAKSLLDEAEAK
VFEIAEAGARHASGFISIQPILKQVVDRVQELYDRDSPSEVTGIPSGFVDLDDKTSGLQPSDMLIVAGRPAMGKTTFALN
VAEHVAVEARLPVAIFSMEMPGTQLATRFISSVGRIDMQKIRSGRLTDEDWQRLTVAMGKLYDAPLYIDETPGLNPIDLR
ARARRLARQCGKLGLIVIDYLQLMTGSKDSDNRAAELSEISRSIKALAKELHVPIIALSQLNRSLEQRPNKRPVMSDLRE
SGAIEQDADIIMFIYRDEVYNPDSPDKGTAELIIGKHRNGPTGVIRMTFLGQYTRFENFAGGGFYTADE

Sequences:

>Translated_469_residues
MSSTSRRFQDGPHDPEMASIKLPPHSLEAEQSLIGGILIDNAAWERIADLVNEADFYRDDHRRIYRHITRLIDLGKPADV
VTVFESLEKNGEAEQAGGLVYLAEIANNTPSAANIRRYAEIVRERAILRKLVAVGDEIAASALTPSGKDAKSLLDEAEAK
VFEIAEAGARHASGFISIQPILKQVVDRVQELYDRDSPSEVTGIPSGFVDLDDKTSGLQPSDMLIVAGRPAMGKTTFALN
VAEHVAVEARLPVAIFSMEMPGTQLATRFISSVGRIDMQKIRSGRLTDEDWQRLTVAMGKLYDAPLYIDETPGLNPIDLR
ARARRLARQCGKLGLIVIDYLQLMTGSKDSDNRAAELSEISRSIKALAKELHVPIIALSQLNRSLEQRPNKRPVMSDLRE
SGAIEQDADIIMFIYRDEVYNPDSPDKGTAELIIGKHRNGPTGVIRMTFLGQYTRFENFAGGGFYTADE
>Mature_468_residues
SSTSRRFQDGPHDPEMASIKLPPHSLEAEQSLIGGILIDNAAWERIADLVNEADFYRDDHRRIYRHITRLIDLGKPADVV
TVFESLEKNGEAEQAGGLVYLAEIANNTPSAANIRRYAEIVRERAILRKLVAVGDEIAASALTPSGKDAKSLLDEAEAKV
FEIAEAGARHASGFISIQPILKQVVDRVQELYDRDSPSEVTGIPSGFVDLDDKTSGLQPSDMLIVAGRPAMGKTTFALNV
AEHVAVEARLPVAIFSMEMPGTQLATRFISSVGRIDMQKIRSGRLTDEDWQRLTVAMGKLYDAPLYIDETPGLNPIDLRA
RARRLARQCGKLGLIVIDYLQLMTGSKDSDNRAAELSEISRSIKALAKELHVPIIALSQLNRSLEQRPNKRPVMSDLRES
GAIEQDADIIMFIYRDEVYNPDSPDKGTAELIIGKHRNGPTGVIRMTFLGQYTRFENFAGGGFYTADE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=450, Percent_Identity=54.4444444444444, Blast_Score=507, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51771; Mature: 51640

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTSRRFQDGPHDPEMASIKLPPHSLEAEQSLIGGILIDNAAWERIADLVNEADFYRDD
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHH
HRRIYRHITRLIDLGKPADVVTVFESLEKNGEAEQAGGLVYLAEIANNTPSAANIRRYAE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCEEEEEEHHCCCCCHHHHHHHHH
IVRERAILRKLVAVGDEIAASALTPSGKDAKSLLDEAEAKVFEIAEAGARHASGFISIQP
HHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEHHH
ILKQVVDRVQELYDRDSPSEVTGIPSGFVDLDDKTSGLQPSDMLIVAGRPAMGKTTFALN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCEEEEE
VAEHVAVEARLPVAIFSMEMPGTQLATRFISSVGRIDMQKIRSGRLTDEDWQRLTVAMGK
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LYDAPLYIDETPGLNPIDLRARARRLARQCGKLGLIVIDYLQLMTGSKDSDNRAAELSEI
HHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
SRSIKALAKELHVPIIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADIIMFIYRDEVY
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCC
NPDSPDKGTAELIIGKHRNGPTGVIRMTFLGQYTRFENFAGGGFYTADE
CCCCCCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SSTSRRFQDGPHDPEMASIKLPPHSLEAEQSLIGGILIDNAAWERIADLVNEADFYRDD
CCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHH
HRRIYRHITRLIDLGKPADVVTVFESLEKNGEAEQAGGLVYLAEIANNTPSAANIRRYAE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCEEEEEEHHCCCCCHHHHHHHHH
IVRERAILRKLVAVGDEIAASALTPSGKDAKSLLDEAEAKVFEIAEAGARHASGFISIQP
HHHHHHHHHHHHHHCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEHHH
ILKQVVDRVQELYDRDSPSEVTGIPSGFVDLDDKTSGLQPSDMLIVAGRPAMGKTTFALN
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCEEEEE
VAEHVAVEARLPVAIFSMEMPGTQLATRFISSVGRIDMQKIRSGRLTDEDWQRLTVAMGK
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LYDAPLYIDETPGLNPIDLRARARRLARQCGKLGLIVIDYLQLMTGSKDSDNRAAELSEI
HHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHH
SRSIKALAKELHVPIIALSQLNRSLEQRPNKRPVMSDLRESGAIEQDADIIMFIYRDEVY
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCC
NPDSPDKGTAELIIGKHRNGPTGVIRMTFLGQYTRFENFAGGGFYTADE
CCCCCCCCCEEEEEECCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]