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The map label for this gene is mutB [H]

Identifier: 119896976

GI number: 119896976

Start: 722387

End: 724552

Strand: Direct

Name: mutB [H]

Synonym: azo0685

Alternate gene names: 119896976

Gene position: 722387-724552 (Clockwise)

Preceding gene: 119896974

Following gene: 119896977

Centisome position: 16.51

GC content: 66.02

Gene sequence:

>2166_bases
ATGTCGAACGCCAACCTGCCCGCCTATCCGCAACCGGATCTCGAGGCCTGGAACAAGGCCGCCGCCAAGCAGGCGCCGGA
CGGCCAACTGGACAAGCTGAACTGGATCACGCCGGAAGGCCTGACCGTCAAGCCGCTGTACACCAAGGCTGACACCGCCG
ACCTGCCGCACGCCGACACCCTGCCGGGCTTCGAGCCCTTCCTGCGCGGCCCGCAGCCCACGATGTACGCGGTCAAGCCG
TGGACCATCCGCCAGTACGCCGGCTTCTCCACCGCGGAAGAATCCAACGCCTTCTACCGCAAGGCGCTCGCCGCCGGCGG
CCAGGGCGTGTCGGTGGCCTTCGACCTCGCCACCCACCGCGGCTACGACTCCGACAATCCCCGCGTGCTCGGCGACGTCG
GCAAGGCCGGCGTGGCGATCGACTCGGTGGAAGACATGAAGATCCTGTTCGACGGCATCCCGCTCGACAAGATCTCGGTG
TCGATGACCATGAACGGCGCGGTGCTGCCCATCCTCGCCGGCTACATCGTCGCGGCCGAAGAGCAGGGCGTGGCGCAGGA
CAAGCTGTCCGGGACCATCCAGAACGACATCCTCAAGGAGTTCATGGTCCGCAACACCTACATCTACCCGCCGACGCCGT
CGATGAAGATCATCGCCGACATCTTCCAGTACACGTCGTCGCACATGCCCAAGTTCAACAGCATCTCGATCTCGGGCTAC
CACATCCAGGAAGCGGGCGCGAACCAGGCCATCGAACTGGCCTTCACGCTCGCCGACGGTCTCGAATACGTGCGCACCGG
CATCGCCGCCGGCATGGATGTCGACACCTTCGCCGGCCGCCTGTCCTTCTTCTGGGCGGTGGGCATGAACTTCTACCTCG
AGATCGCCAAGATGCGCGCTGCGCGCCTGCTGTGGTGGCGGATCATGAAGCAGTTCAACCCCAAGAGCCCCAAGTCGATG
ATGCTGCGCACCCACTCGCAGACTTCCGGCTGGTCGCTCACCGAGCAGGACCCGTACAACAACGTGGTGCGCACCACCAT
CGAGGCGATGGCCGCGGTGTTCGGCGGCACCCAGTCGCTGCACACCAACGCGCTCGACGAAGCGATCGCGCTGCCCACCG
AGTTCTCGGCCCGCATCGCTCGCAACACCCAGATCATCATCCAGGAAGAGACCCACATCTGTAACGTCGTCGATCCCTGG
GCCGGCTCCTACATGATGGAAAAGCTCACCCAGGACATGGCAGACAAGGCCTGGGCGCTGATCGAGGAAATCGAGGCCAT
GGGCGGCATGACCAAGGCGGTCGAATCCGGCTGGGCCAAGATGAAGGTCGAGGAATGCGCCGCCGACAAGCAGGCGCGCA
TCGACTCCGGCAAGGACGTCATCGTCGGCGTGAACAAGTACAAGCTGGCGAAGGAAGACCCGATCGAGATCCTCGACATC
GACAACCACGCCGTGCGTGAAGCGCAGATCGCCCGTCTCACCAAGATCCGCGCCACCCGCGACAGCGCCGCGGTGCAGGC
CGCGCTCGACGCGCTCACCCAGTGCGCCGAAAGCGGCGAAGGCAACCTGCTCGACCTCGCGGTCAAGGCGGTGCGTCTGC
GCGCCTCGGTGGGCGAAATCTCCGACGCGCTGGAAAAGGTGTTCGGCCGCTACCGTGCCAACCCGCAGGCGGTGTCCGGC
GTGTATGGCGCCGTGGTGGAAGACGACGCCGACTGGAAGGAACTGAAGGCCGAGATCGAAGCCTTCATCGCCGAGGAAGG
CCGCCGCCCCCGCATCATGATCGCCAAGCTCGGCCAGGACGGCCACGACCGTGGCGCCAAGGTGGTGGCTTCCGCCTTCG
CCGACCTCGGCTTCGACATCGACATCGGCCCGCTCTTCCAGACCCCGGAAGAAGCGGCCCGCCACGCGGTGGAAAACGAT
GTGCACGCGGTCGGCTGCTCCAGCCTCGCCGCCGGCCACAAGACCCTGGTGCCGGCCATCATCGCCGAGCTGAAGCGCTT
GGGCGCCGAAGACATCATCACCTTCGTCGGTGGCGTGATCCCGGCGCAGGACTACGACGCGCTCTACGCCGCCGGTGCCA
AGGGCATCTTCGGCCCGGGTACGCCGATCCCGAAGGCGGCGCGCGAAGTGCTGAAGCAGATCCGCGCGGCCAAGGTCGCG
GCCTGA

Upstream 100 bases:

>100_bases
GCGGTATGGTTCGCGGGCGGTCTCGTCGTCGCCGGTCGGTGCTTGTCACACATACATCTATGCCGCTGCGGCGGCGCTAC
TGATTCAGTGAGGGTTAGTC

Downstream 100 bases:

>100_bases
TACGCTTCGCTGCCCGCAACTCGCCGTCATGGCGGGGTGCGGGCGCAGCCCGTCTCCGTCCGCCCGATGGCCATGAACAT
GCCCGAAAGCCTGCCCGTGC

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MSNANLPAYPQPDLEAWNKAAAKQAPDGQLDKLNWITPEGLTVKPLYTKADTADLPHADTLPGFEPFLRGPQPTMYAVKP
WTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIPLDKISV
SMTMNGAVLPILAGYIVAAEEQGVAQDKLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFQYTSSHMPKFNSISISGY
HIQEAGANQAIELAFTLADGLEYVRTGIAAGMDVDTFAGRLSFFWAVGMNFYLEIAKMRAARLLWWRIMKQFNPKSPKSM
MLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQIIIQEETHICNVVDPW
AGSYMMEKLTQDMADKAWALIEEIEAMGGMTKAVESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKLAKEDPIEILDI
DNHAVREAQIARLTKIRATRDSAAVQAALDALTQCAESGEGNLLDLAVKAVRLRASVGEISDALEKVFGRYRANPQAVSG
VYGAVVEDDADWKELKAEIEAFIAEEGRRPRIMIAKLGQDGHDRGAKVVASAFADLGFDIDIGPLFQTPEEAARHAVEND
VHAVGCSSLAAGHKTLVPAIIAELKRLGAEDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPKAAREVLKQIRAAKVA
A

Sequences:

>Translated_721_residues
MSNANLPAYPQPDLEAWNKAAAKQAPDGQLDKLNWITPEGLTVKPLYTKADTADLPHADTLPGFEPFLRGPQPTMYAVKP
WTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIPLDKISV
SMTMNGAVLPILAGYIVAAEEQGVAQDKLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFQYTSSHMPKFNSISISGY
HIQEAGANQAIELAFTLADGLEYVRTGIAAGMDVDTFAGRLSFFWAVGMNFYLEIAKMRAARLLWWRIMKQFNPKSPKSM
MLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQIIIQEETHICNVVDPW
AGSYMMEKLTQDMADKAWALIEEIEAMGGMTKAVESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKLAKEDPIEILDI
DNHAVREAQIARLTKIRATRDSAAVQAALDALTQCAESGEGNLLDLAVKAVRLRASVGEISDALEKVFGRYRANPQAVSG
VYGAVVEDDADWKELKAEIEAFIAEEGRRPRIMIAKLGQDGHDRGAKVVASAFADLGFDIDIGPLFQTPEEAARHAVEND
VHAVGCSSLAAGHKTLVPAIIAELKRLGAEDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPKAAREVLKQIRAAKVA
A
>Mature_720_residues
SNANLPAYPQPDLEAWNKAAAKQAPDGQLDKLNWITPEGLTVKPLYTKADTADLPHADTLPGFEPFLRGPQPTMYAVKPW
TIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHRGYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIPLDKISVS
MTMNGAVLPILAGYIVAAEEQGVAQDKLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFQYTSSHMPKFNSISISGYH
IQEAGANQAIELAFTLADGLEYVRTGIAAGMDVDTFAGRLSFFWAVGMNFYLEIAKMRAARLLWWRIMKQFNPKSPKSMM
LRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQIIIQEETHICNVVDPWA
GSYMMEKLTQDMADKAWALIEEIEAMGGMTKAVESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKLAKEDPIEILDID
NHAVREAQIARLTKIRATRDSAAVQAALDALTQCAESGEGNLLDLAVKAVRLRASVGEISDALEKVFGRYRANPQAVSGV
YGAVVEDDADWKELKAEIEAFIAEEGRRPRIMIAKLGQDGHDRGAKVVASAFADLGFDIDIGPLFQTPEEAARHAVENDV
HAVGCSSLAAGHKTLVPAIIAELKRLGAEDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPKAAREVLKQIRAAKVAA

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=705, Percent_Identity=66.241134751773, Blast_Score=977, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=708, Percent_Identity=61.864406779661, Blast_Score=885, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=707, Percent_Identity=64.6393210749646, Blast_Score=960, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78196; Mature: 78065

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNANLPAYPQPDLEAWNKAAAKQAPDGQLDKLNWITPEGLTVKPLYTKADTADLPHADT
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
LPGFEPFLRGPQPTMYAVKPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHR
CCCCHHHHCCCCCCEEEECCEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHC
GYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIPLDKISVSMTMNGAVLPILAGYIVAAE
CCCCCCCEEEECCCCCCEEECCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHEEEC
EQGVAQDKLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFQYTSSHMPKFNSISISGY
CCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECE
HIQEAGANQAIELAFTLADGLEYVRTGIAAGMDVDTFAGRLSFFWAVGMNFYLEIAKMRA
EECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLWWRIMKQFNPKSPKSMMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSL
HHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
HTNALDEAIALPTEFSARIARNTQIIIQEETHICNVVDPWAGSYMMEKLTQDMADKAWAL
HHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
IEEIEAMGGMTKAVESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKLAKEDPIEILDI
HHHHHHHCCHHHHHHCCHHHHHHHHHHCCHHHCCCCCCEEEEECCCEECCCCCCCEEEEC
DNHAVREAQIARLTKIRATRDSAAVQAALDALTQCAESGEGNLLDLAVKAVRLRASVGEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SDALEKVFGRYRANPQAVSGVYGAVVEDDADWKELKAEIEAFIAEEGRRPRIMIAKLGQD
HHHHHHHHHHHCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GHDRGAKVVASAFADLGFDIDIGPLFQTPEEAARHAVENDVHAVGCSSLAAGHKTLVPAI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCHHHHHCCHHHHHHHH
IAELKRLGAEDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPKAAREVLKQIRAAKVA
HHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
A
C
>Mature Secondary Structure 
SNANLPAYPQPDLEAWNKAAAKQAPDGQLDKLNWITPEGLTVKPLYTKADTADLPHADT
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
LPGFEPFLRGPQPTMYAVKPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLATHR
CCCCHHHHCCCCCCEEEECCEEEHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEHHHC
GYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIPLDKISVSMTMNGAVLPILAGYIVAAE
CCCCCCCEEEECCCCCCEEECCHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHEEEC
EQGVAQDKLSGTIQNDILKEFMVRNTYIYPPTPSMKIIADIFQYTSSHMPKFNSISISGY
CCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECE
HIQEAGANQAIELAFTLADGLEYVRTGIAAGMDVDTFAGRLSFFWAVGMNFYLEIAKMRA
EECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARLLWWRIMKQFNPKSPKSMMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQSL
HHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
HTNALDEAIALPTEFSARIARNTQIIIQEETHICNVVDPWAGSYMMEKLTQDMADKAWAL
HHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHH
IEEIEAMGGMTKAVESGWAKMKVEECAADKQARIDSGKDVIVGVNKYKLAKEDPIEILDI
HHHHHHHCCHHHHHHCCHHHHHHHHHHCCHHHCCCCCCEEEEECCCEECCCCCCCEEEEC
DNHAVREAQIARLTKIRATRDSAAVQAALDALTQCAESGEGNLLDLAVKAVRLRASVGEI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SDALEKVFGRYRANPQAVSGVYGAVVEDDADWKELKAEIEAFIAEEGRRPRIMIAKLGQD
HHHHHHHHHHHCCCHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GHDRGAKVVASAFADLGFDIDIGPLFQTPEEAARHAVENDVHAVGCSSLAAGHKTLVPAI
CCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCHHHHCHHHHHCCHHHHHHHH
IAELKRLGAEDIITFVGGVIPAQDYDALYAAGAKGIFGPGTPIPKAAREVLKQIRAAKVA
HHHHHHCCHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8566763; 12949112 [H]