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The map label for this gene is napA1 [H]

Identifier: 119896962

GI number: 119896962

Start: 705728

End: 708220

Strand: Reverse

Name: napA1 [H]

Synonym: azo0671

Alternate gene names: 119896962

Gene position: 708220-705728 (Counterclockwise)

Preceding gene: 119896963

Following gene: 119896961

Centisome position: 16.18

GC content: 67.11

Gene sequence:

>2493_bases
ATGAAGCTGACCCGCCGCGCGTTCATTAAGCAGTCCGCCGCCCTCAGCACCGCTGCTGCGGCCGGCATCCCGGCCGAAAC
GCTGGGCGCCAACATCGTCACCGACGCCGCGCTGACCAAGCTCGCCTGGCACAAGGCGCCCTGTCGCTTCTGCGGCGTGG
GCTGCGGGGTGAACGTGGCGGTGCGCGACAACCGCGTCGTCGCCACCCACGGCGACATCAACGCCGAGGTCAATCGCGGC
ATCAACTGCGTCAAGGGCTACTTCCTGTCCAAGATCATGTACGGCGCGGACCGCCTGACCGAGCCGCTGCTGCGCCAGAC
CAACGGCCAGTACGACAAGAACGGCGAACTGCAGCCGGTGAGCTGGGAGCGCGCCTTCGACGTGATGGCCGAGCAGTTCA
AGAAGGCGCTGCGCGAGAAGGGGCCGTCCGGCGTGGGCATGTTCGGCTCGGGCCAATGGACCATCTGGGAAGGTTACGCC
GCCGCCAAGCTCTACAAGGCAGGCTTCCGCTCCAACAACATCGACCCCAACGCGCGCCATTGCATGGCCTCCGCCGTGGC
CGGCTTCATGCGCACCTTCGGCATGGACGAACCGATGGGCTGCTACGACGACTTCGAGCAGGCCGACGCCTTCGTGCTGT
GGGGCGCGAACATGGCCGAGATGCACCCCATCCTGTGGACGCGGCTGACCGACCGCCGGCTGTCCGCGCCGCAGTCGAGG
GTCGCGGTGCTGTCGGTGTTCGAACACCGCTGTTACGACCTCGCCGACCTGACGCTGACCTTCCGCCCCCAGACCGACCT
CGCGATCCTCAACTACATCGCCAACTACATCATTCAGCGCGGCGCTGTGAATCGCGACTTCATCAACCGCCACACCCGCT
TCATGACCGGTAACACCGACATCGGTTACGGCCTGCGGCCGGAGCACCCGCTGCAGAAGGCCAAGAACGTCGCCGCGCCG
GGCGGCGGACAGCCGAGCACGTTCGACGAGTACGCCGCCTTCGTGGCCAAGTACGACGTGGACACGGTGGCGCGCCTGTC
CAATGTGCCGAAGGACCGGCTGGAGCAGCTCGCGCAGCTCTACGCCGATCCCAAGGTCAAGGTGATGTCGCTGTTCACGA
TGGGCTTCAACCAGCACACGCGCGGCACCTGGTGCAGCCACCTGCTGTACAACCTGCACCTGCTCACCGGCAAGATCTCG
TCGCCGGGCAACGGGCCGTTCTCGCTCACCGGCCAGCCCTCGGCCTGCGGCACCGCGCGCGAGGTCGGCACCTTCTCGCA
CCGGCTGCCCGCCGACATGGTCGTCACCAATCCGCAGCACCGCGAAACCGCGGAACGCATCTGGAAGCTGCCGGCAGGCA
CCATCCCCGACAAGCCGGGCTACCACGCGGTGTTGCAGAACCGCATGCTCAAGGACGGCCAGCTGCAGGCCTACTGGGTG
ATGTGCAACAACAACATGCAGGCCGCGCCCAACATCGCGCAGGAAGCCTGGCCCGGCTACCGCAACCCGTCCAACTTCGT
CGTGGTGTCCGACGCCTACCCGACCATGACCACGGTGGCGGCGGACCTGGTGCTGCCAACGGCGATGTGGGTGGAGAAAG
AAGGCGCCTACGGCAACGCCGAGCGCCGCACCCAGTTCTGGCACCAGCTGGTGCAGGCGCCCGGACAGGCGCGTTCGGAC
CTTTGGCAGCTGATGGAATTCTCCAAGCGCTTCCGCATGGAAGAAGTGTGGCCGGCCGAACTGCTGGCCAAGGCGCCGCA
GTACCGCGGCAAGACCTTGTTCGACGTGCTGTTCCGCAACGGCGAGGTCGACAAGTTCCCCGCCACGCAGGTCGAGGCCG
GCTACGCCAACGACGAAGCCCGCGCCTTCGGCTTCTACCCGCAGAAGGGGCTGTTCGAGGAGTACGCCCAGTTCGGCCGC
GGCCACGGCCACGACCTCGCGCCTTTCGACACCTACCACCAGGTGCGCGGGCTGCGCTGGCCGGTGGTAAACGGCCAGGA
AACGCGCTGGCGCTACCGCGAGGGCCTGGACCCCTACGTGAAGGCCGGCGAGGACGTGAAGTTCTACGGCATGCCGGACG
GCAAGGCGGTGATCTACGCCCTGCCCTACGAACCCGCGGCCGAGGAGCCGGACAAGGAGTACCCGTTCTGGCTATGCACC
GGCCGTGTGCTGGAACACTGGCATTCCGGCTCGATGACGCAGCGCGTGCCGGAACTGCACCGCGCCTTCCCGAATGCGGT
GTGCTTCATGCACCCGGACGACACCCGCAAGCTCGGCCTGCGCCGCGGCGACGAGATCGAGGTGGTGTCGCGCCGCGGGG
TCATGCGCACCCGGGTGGAGACGCGCGGCCGCAACAAGCCGCCACCGGGCCTGGTGTTCGTGCCCTGGTTCGATGCCAGC
CAGCTGATCAACAAGGTGACGCTGGACGCGACCTGCCCGATCTCGCTGCAGACGGACTTCAAGAAGTGCGCGGTGAACAT
CCGCAAGGTCTAG

Upstream 100 bases:

>100_bases
GCCCTGCTCGACCGCATCCGCGATCTCGCCGGGGTGGCCAATGTCGCGCTGGTGTACCAGCACACCGAGACCGGCGCCGA
GATGGATGAGGAGATCAACG

Downstream 100 bases:

>100_bases
GAGAACAGCCATGCGCATCCTGCTTCCCTTCGCGCTGGCTGCCGCCTTCGCCGGCGCCGCGCTCGCCCAGCATGTCGACA
ACGCCCGCGGCCCCACGCCG

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 830; Mature: 830

Protein sequence:

>830_residues
MKLTRRAFIKQSAALSTAAAAGIPAETLGANIVTDAALTKLAWHKAPCRFCGVGCGVNVAVRDNRVVATHGDINAEVNRG
INCVKGYFLSKIMYGADRLTEPLLRQTNGQYDKNGELQPVSWERAFDVMAEQFKKALREKGPSGVGMFGSGQWTIWEGYA
AAKLYKAGFRSNNIDPNARHCMASAVAGFMRTFGMDEPMGCYDDFEQADAFVLWGANMAEMHPILWTRLTDRRLSAPQSR
VAVLSVFEHRCYDLADLTLTFRPQTDLAILNYIANYIIQRGAVNRDFINRHTRFMTGNTDIGYGLRPEHPLQKAKNVAAP
GGGQPSTFDEYAAFVAKYDVDTVARLSNVPKDRLEQLAQLYADPKVKVMSLFTMGFNQHTRGTWCSHLLYNLHLLTGKIS
SPGNGPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPQHRETAERIWKLPAGTIPDKPGYHAVLQNRMLKDGQLQAYWV
MCNNNMQAAPNIAQEAWPGYRNPSNFVVVSDAYPTMTTVAADLVLPTAMWVEKEGAYGNAERRTQFWHQLVQAPGQARSD
LWQLMEFSKRFRMEEVWPAELLAKAPQYRGKTLFDVLFRNGEVDKFPATQVEAGYANDEARAFGFYPQKGLFEEYAQFGR
GHGHDLAPFDTYHQVRGLRWPVVNGQETRWRYREGLDPYVKAGEDVKFYGMPDGKAVIYALPYEPAAEEPDKEYPFWLCT
GRVLEHWHSGSMTQRVPELHRAFPNAVCFMHPDDTRKLGLRRGDEIEVVSRRGVMRTRVETRGRNKPPPGLVFVPWFDAS
QLINKVTLDATCPISLQTDFKKCAVNIRKV

Sequences:

>Translated_830_residues
MKLTRRAFIKQSAALSTAAAAGIPAETLGANIVTDAALTKLAWHKAPCRFCGVGCGVNVAVRDNRVVATHGDINAEVNRG
INCVKGYFLSKIMYGADRLTEPLLRQTNGQYDKNGELQPVSWERAFDVMAEQFKKALREKGPSGVGMFGSGQWTIWEGYA
AAKLYKAGFRSNNIDPNARHCMASAVAGFMRTFGMDEPMGCYDDFEQADAFVLWGANMAEMHPILWTRLTDRRLSAPQSR
VAVLSVFEHRCYDLADLTLTFRPQTDLAILNYIANYIIQRGAVNRDFINRHTRFMTGNTDIGYGLRPEHPLQKAKNVAAP
GGGQPSTFDEYAAFVAKYDVDTVARLSNVPKDRLEQLAQLYADPKVKVMSLFTMGFNQHTRGTWCSHLLYNLHLLTGKIS
SPGNGPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPQHRETAERIWKLPAGTIPDKPGYHAVLQNRMLKDGQLQAYWV
MCNNNMQAAPNIAQEAWPGYRNPSNFVVVSDAYPTMTTVAADLVLPTAMWVEKEGAYGNAERRTQFWHQLVQAPGQARSD
LWQLMEFSKRFRMEEVWPAELLAKAPQYRGKTLFDVLFRNGEVDKFPATQVEAGYANDEARAFGFYPQKGLFEEYAQFGR
GHGHDLAPFDTYHQVRGLRWPVVNGQETRWRYREGLDPYVKAGEDVKFYGMPDGKAVIYALPYEPAAEEPDKEYPFWLCT
GRVLEHWHSGSMTQRVPELHRAFPNAVCFMHPDDTRKLGLRRGDEIEVVSRRGVMRTRVETRGRNKPPPGLVFVPWFDAS
QLINKVTLDATCPISLQTDFKKCAVNIRKV
>Mature_830_residues
MKLTRRAFIKQSAALSTAAAAGIPAETLGANIVTDAALTKLAWHKAPCRFCGVGCGVNVAVRDNRVVATHGDINAEVNRG
INCVKGYFLSKIMYGADRLTEPLLRQTNGQYDKNGELQPVSWERAFDVMAEQFKKALREKGPSGVGMFGSGQWTIWEGYA
AAKLYKAGFRSNNIDPNARHCMASAVAGFMRTFGMDEPMGCYDDFEQADAFVLWGANMAEMHPILWTRLTDRRLSAPQSR
VAVLSVFEHRCYDLADLTLTFRPQTDLAILNYIANYIIQRGAVNRDFINRHTRFMTGNTDIGYGLRPEHPLQKAKNVAAP
GGGQPSTFDEYAAFVAKYDVDTVARLSNVPKDRLEQLAQLYADPKVKVMSLFTMGFNQHTRGTWCSHLLYNLHLLTGKIS
SPGNGPFSLTGQPSACGTAREVGTFSHRLPADMVVTNPQHRETAERIWKLPAGTIPDKPGYHAVLQNRMLKDGQLQAYWV
MCNNNMQAAPNIAQEAWPGYRNPSNFVVVSDAYPTMTTVAADLVLPTAMWVEKEGAYGNAERRTQFWHQLVQAPGQARSD
LWQLMEFSKRFRMEEVWPAELLAKAPQYRGKTLFDVLFRNGEVDKFPATQVEAGYANDEARAFGFYPQKGLFEEYAQFGR
GHGHDLAPFDTYHQVRGLRWPVVNGQETRWRYREGLDPYVKAGEDVKFYGMPDGKAVIYALPYEPAAEEPDKEYPFWLCT
GRVLEHWHSGSMTQRVPELHRAFPNAVCFMHPDDTRKLGLRRGDEIEVVSRRGVMRTRVETRGRNKPPPGLVFVPWFDAS
QLINKVTLDATCPISLQTDFKKCAVNIRKV

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788534, Length=830, Percent_Identity=68.1927710843374, Blast_Score=1220, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=802, Percent_Identity=22.4438902743142, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI3868720, Length=662, Percent_Identity=23.1117824773414, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI3868719, Length=512, Percent_Identity=22.65625, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI87081797, Length=905, Percent_Identity=20.5524861878453, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1787870, Length=630, Percent_Identity=22.0634920634921, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 93282; Mature: 93282

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTRRAFIKQSAALSTAAAAGIPAETLGANIVTDAALTKLAWHKAPCRFCGVGCGVNVA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEE
VRDNRVVATHGDINAEVNRGINCVKGYFLSKIMYGADRLTEPLLRQTNGQYDKNGELQPV
EECCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SWERAFDVMAEQFKKALREKGPSGVGMFGSGQWTIWEGYAAAKLYKAGFRSNNIDPNARH
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVAGFMRTFGMDEPMGCYDDFEQADAFVLWGANMAEMHPILWTRLTDRRLSAPQSR
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHCHHHHHHHHHHHHCCCHHH
VAVLSVFEHRCYDLADLTLTFRPQTDLAILNYIANYIIQRGAVNRDFINRHTRFMTGNTD
HHHHHHHHHHHCCEECEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEECCCC
IGYGLRPEHPLQKAKNVAAPGGGQPSTFDEYAAFVAKYDVDTVARLSNVPKDRLEQLAQL
CCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
YADPKVKVMSLFTMGFNQHTRGTWCSHLLYNLHLLTGKISSPGNGPFSLTGQPSACGTAR
HCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCEEECCCCCCCCCHH
EVGTFSHRLPADMVVTNPQHRETAERIWKLPAGTIPDKPGYHAVLQNRMLKDGQLQAYWV
HHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEE
MCNNNMQAAPNIAQEAWPGYRNPSNFVVVSDAYPTMTTVAADLVLPTAMWVEKEGAYGNA
EECCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCH
ERRTQFWHQLVQAPGQARSDLWQLMEFSKRFRMEEVWPAELLAKAPQYRGKTLFDVLFRN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHCCCCCCCCHHHHHHHCC
GEVDKFPATQVEAGYANDEARAFGFYPQKGLFEEYAQFGRGHGHDLAPFDTYHQVRGLRW
CCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
PVVNGQETRWRYREGLDPYVKAGEDVKFYGMPDGKAVIYALPYEPAAEEPDKEYPFWLCT
CCCCCCHHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEH
GRVLEHWHSGSMTQRVPELHRAFPNAVCFMHPDDTRKLGLRRGDEIEVVSRRGVMRTRVE
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCEEHHHHCCHHHHHHH
TRGRNKPPPGLVFVPWFDASQLINKVTLDATCPISLQTDFKKCAVNIRKV
HCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MKLTRRAFIKQSAALSTAAAAGIPAETLGANIVTDAALTKLAWHKAPCRFCGVGCGVNVA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCCEEEE
VRDNRVVATHGDINAEVNRGINCVKGYFLSKIMYGADRLTEPLLRQTNGQYDKNGELQPV
EECCEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SWERAFDVMAEQFKKALREKGPSGVGMFGSGQWTIWEGYAAAKLYKAGFRSNNIDPNARH
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCCHHHH
CMASAVAGFMRTFGMDEPMGCYDDFEQADAFVLWGANMAEMHPILWTRLTDRRLSAPQSR
HHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECCCHHHHCHHHHHHHHHHHHCCCHHH
VAVLSVFEHRCYDLADLTLTFRPQTDLAILNYIANYIIQRGAVNRDFINRHTRFMTGNTD
HHHHHHHHHHHCCEECEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCEEEECCCC
IGYGLRPEHPLQKAKNVAAPGGGQPSTFDEYAAFVAKYDVDTVARLSNVPKDRLEQLAQL
CCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHH
YADPKVKVMSLFTMGFNQHTRGTWCSHLLYNLHLLTGKISSPGNGPFSLTGQPSACGTAR
HCCCCEEEEEHHHHCCCCCCCHHHHHHHHHHHHHEEEECCCCCCCCEEECCCCCCCCCHH
EVGTFSHRLPADMVVTNPQHRETAERIWKLPAGTIPDKPGYHAVLQNRMLKDGQLQAYWV
HHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEE
MCNNNMQAAPNIAQEAWPGYRNPSNFVVVSDAYPTMTTVAADLVLPTAMWVEKEGAYGNA
EECCCCCCCCCHHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCH
ERRTQFWHQLVQAPGQARSDLWQLMEFSKRFRMEEVWPAELLAKAPQYRGKTLFDVLFRN
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCHHHHHHCCCCCCCCHHHHHHHCC
GEVDKFPATQVEAGYANDEARAFGFYPQKGLFEEYAQFGRGHGHDLAPFDTYHQVRGLRW
CCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCC
PVVNGQETRWRYREGLDPYVKAGEDVKFYGMPDGKAVIYALPYEPAAEEPDKEYPFWLCT
CCCCCCHHHHHHHHCCCHHHHCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEH
GRVLEHWHSGSMTQRVPELHRAFPNAVCFMHPDDTRKLGLRRGDEIEVVSRRGVMRTRVE
HHHHHHHCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCEEHHHHCCHHHHHHH
TRGRNKPPPGLVFVPWFDASQLINKVTLDATCPISLQTDFKKCAVNIRKV
HCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8376334; 12948488 [H]