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The map label for this gene is metF [H]

Identifier: 119896875

GI number: 119896875

Start: 615741

End: 616574

Strand: Reverse

Name: metF [H]

Synonym: azo0584

Alternate gene names: 119896875

Gene position: 616574-615741 (Counterclockwise)

Preceding gene: 119896876

Following gene: 119896873

Centisome position: 14.09

GC content: 67.87

Gene sequence:

>834_bases
ATGCACAAGACTGAACTTTCCATCGAATTCTTCCCGCCGCAGACGGCCGAGGGCGCAGAGAAGCTGCGCGCGGTGCAGGC
CCGGCTCGCCGAGCTGAAGCCCACCTTCTTCTCGGTGACCTATGGCGCCGGCGGCTCCACCCGCGAGCGCACCTTCGCCA
CGGTCAAGGAGATCGCCGCCAGCGGCAGCGAGGCCGCGCCCCACCTGTCGTGCATCGGCTCCACCCGCGACAGCATCCGC
GCCATCCTTGCCGAATATGCCGACGCCGGCGTCAAGCGCATCGTCGCGCTGCGCGGCGACCTGCCCTCGGGCGTGGTCGA
TCCGGGCGAGTTCCGCTACGCCAACGAACTGGTGGAATTCATCCGCGCCGAGACCGGCTCGCGCTTCCGCATCGAGGTTG
CCGCCTATCCGGAATGGCATCCGCAGGCCAAGACCCCCGCCGACGACCTTGCCGCCTTCAAGCGCAAGATGGACGCCGGC
GCGGATTCGGCGATCACGCAGTACTTCTACAACCTCGACGCCTACCTGCACTTCGTCGACGCGGTGCGCAAGCTCGGCAT
CGACAAACCGGTGGTGCCGGGCATCATGCCGATCGGCAGCTTCTCCAAGCTGGCGCGCTTCTCGGATGCCTGCGGCGCCG
AGATCCCGCGCTGGATGCGGCGCAAGTTCGAGTCCTACGGCGACGACGCCGACGCCATCCGCGCCTTCGGCCTGGATGTG
GTCACCGAGCTGTGCGAGAAGCTGCTCGCCGCCGGCGTCCCCGGCCTGCACTTCTACAGCATGAACCAGTCCGCGCTCAC
CACCGAGATCTGCAAGCGCCTCGGGCTGGCCTGA

Upstream 100 bases:

>100_bases
CGCATCGCCGCCGCGGCTGCAGCGCTGGACGTGCGCGGCTGGTACGACTCGCCCATCACCGGCACCGACGGCAACCGCGA
ATTCTTCATCTGGATGCGAC

Downstream 100 bases:

>100_bases
GATAGAAGGCCCCCAAGCGGGGGCTCAGCCGAGGCCGGCGGAAGCCGGCGTCGGCGGGCGCATGCCGCCCAGCGCCGGCA
TGCAGGCGCGCATCATCGCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MHKTELSIEFFPPQTAEGAEKLRAVQARLAELKPTFFSVTYGAGGSTRERTFATVKEIAASGSEAAPHLSCIGSTRDSIR
AILAEYADAGVKRIVALRGDLPSGVVDPGEFRYANELVEFIRAETGSRFRIEVAAYPEWHPQAKTPADDLAAFKRKMDAG
ADSAITQYFYNLDAYLHFVDAVRKLGIDKPVVPGIMPIGSFSKLARFSDACGAEIPRWMRRKFESYGDDADAIRAFGLDV
VTELCEKLLAAGVPGLHFYSMNQSALTTEICKRLGLA

Sequences:

>Translated_277_residues
MHKTELSIEFFPPQTAEGAEKLRAVQARLAELKPTFFSVTYGAGGSTRERTFATVKEIAASGSEAAPHLSCIGSTRDSIR
AILAEYADAGVKRIVALRGDLPSGVVDPGEFRYANELVEFIRAETGSRFRIEVAAYPEWHPQAKTPADDLAAFKRKMDAG
ADSAITQYFYNLDAYLHFVDAVRKLGIDKPVVPGIMPIGSFSKLARFSDACGAEIPRWMRRKFESYGDDADAIRAFGLDV
VTELCEKLLAAGVPGLHFYSMNQSALTTEICKRLGLA
>Mature_277_residues
MHKTELSIEFFPPQTAEGAEKLRAVQARLAELKPTFFSVTYGAGGSTRERTFATVKEIAASGSEAAPHLSCIGSTRDSIR
AILAEYADAGVKRIVALRGDLPSGVVDPGEFRYANELVEFIRAETGSRFRIEVAAYPEWHPQAKTPADDLAAFKRKMDAG
ADSAITQYFYNLDAYLHFVDAVRKLGIDKPVVPGIMPIGSFSKLARFSDACGAEIPRWMRRKFESYGDDADAIRAFGLDV
VTELCEKLLAAGVPGLHFYSMNQSALTTEICKRLGLA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=40, Blast_Score=191, Evalue=6e-49,
Organism=Escherichia coli, GI1790377, Length=275, Percent_Identity=42.1818181818182, Blast_Score=233, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI25147436, Length=285, Percent_Identity=34.3859649122807, Blast_Score=166, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI25147441, Length=285, Percent_Identity=34.3859649122807, Blast_Score=166, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6325234, Length=288, Percent_Identity=34.0277777777778, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321313, Length=284, Percent_Identity=33.4507042253521, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI24662658, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30292; Mature: 30292

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKTELSIEFFPPQTAEGAEKLRAVQARLAELKPTFFSVTYGAGGSTRERTFATVKEIAA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
SGSEAAPHLSCIGSTRDSIRAILAEYADAGVKRIVALRGDLPSGVVDPGEFRYANELVEF
CCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IRAETGSRFRIEVAAYPEWHPQAKTPADDLAAFKRKMDAGADSAITQYFYNLDAYLHFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AVRKLGIDKPVVPGIMPIGSFSKLARFSDACGAEIPRWMRRKFESYGDDADAIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTELCEKLLAAGVPGLHFYSMNQSALTTEICKRLGLA
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MHKTELSIEFFPPQTAEGAEKLRAVQARLAELKPTFFSVTYGAGGSTRERTFATVKEIAA
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHH
SGSEAAPHLSCIGSTRDSIRAILAEYADAGVKRIVALRGDLPSGVVDPGEFRYANELVEF
CCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IRAETGSRFRIEVAAYPEWHPQAKTPADDLAAFKRKMDAGADSAITQYFYNLDAYLHFVD
HHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
AVRKLGIDKPVVPGIMPIGSFSKLARFSDACGAEIPRWMRRKFESYGDDADAIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VTELCEKLLAAGVPGLHFYSMNQSALTTEICKRLGLA
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]