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The map label for this gene is emrY [H]

Identifier: 119896869

GI number: 119896869

Start: 607889

End: 609352

Strand: Direct

Name: emrY [H]

Synonym: azo0578

Alternate gene names: 119896869

Gene position: 607889-609352 (Clockwise)

Preceding gene: 119896867

Following gene: 119896870

Centisome position: 13.89

GC content: 73.22

Gene sequence:

>1464_bases
GTGTTGCTCAGCGCCCACCCCGAGCTGCGGCCGCTGCGCGTCCATGCCTTCGGGGCCACGCTGGGACTGGTGGCCGGGGT
GGATTTCGTTGCCGCGATCCTGATCGCGATGGCGGGCACCCAGGTCCAGCACGGCCTGGGGGTGGCGCCGCGCGACTTCC
TGTGGGCGCTGACCAGCTACGCGGTGGCGGCGGTGATGGCCAACCTGATGCTGGTGCAGCTGGCGGGGCGGGTGAACTAC
CGCCGCTTCACGCTCGCCAGCCTGGCGGTCTTCATCGCCGGCGCGCTCGGCTGCGCGGCGGCCGATTCGCTGCCGGCCTT
CACCGCGGCCCGCCTGGTGCAGGGCCTGGGTGGCGGCGGGCTGTTCGCGGCGGCACGCATCCTGGCCCAGTACAGCTCGC
AGCCGCAGGAGCGGCTGTCGCTGTTCTGGGGCTTCGGCGTGGCCAACTTCGCGCTCGTCGCGCTCGCACCCTGGCTGGCG
GGCTGGCTGGTGGCCCATCACGGCTGGCACACCCTGTTCCTGCTGCAGGCGGGGCTGGCGCTGCCCTTGCTGCCGCTGGT
GGCGTGGCTGTATCCGCGCGTCGAGCGCACCCCGTCGCGCGCCCTCGGCCGGCTGGACTGGCCCGGCGTGCTGGCCTTCG
CCGGCGGGGCGCTGCTGCTGGTCCATGCGCTGGAGCACCTGCGCTATCTCAGCCGTGCGGAACTGCCGCAGCTGGCCGCC
TACGCGCTGCTCGGGCTGGTGCTGCTGGGGGTGGTGATGCGGCGCTTCGCGCGCCATCCCGACCCTTGGCTCGATCCGCG
CCGGCTGGCCAGCCGCCGCTACCTGGCCGGGCTCGCCTTCTACGGCGTGTTCTATCTGATCTGCGGCTACTGGAACTTCC
TGGTCCCGGCGGTGCTGGTCGACGGCCTGGGCTTCGGCCTCGACACGGTGGGCGGGCTGCTCGCGCTCGGCGGGCTCACC
ACGCTGGGTGCGGTGGTCGTCTTCCACCTCTGCCTGCCCTACATCATGCGCAAGCGCCGCTATATCGCGCTCGGCTATGT
CGGCCTCGCGGCCGCCTTCACGCTGATGGCCGTCAGCGCGCGGCCCGGCGCCGGGGTGCTGGACGTGCTGCCGCCCATCG
TGCTGCAGGGGGCGATGCCGGTGCTGGCGCTGCTGCAGGTGGCCATGATGACCTTCGTCGACATGCCGACCGAGGACTTC
GCCCACGCCTACGCGTTCAAGAATATCGTCCGCGAGATCCTCAACGCGCTCGGCACCGGGCTCGCGGTGCTTCAGCTGCA
CCACGGCACCGCGGCGGCGCAGGCGGCGATGCAGGCCGCCACCCCCGGCCCGCTGCAGCCCCACCTGGCCGAGGTGGTGC
ATCGGGCGGCGGTGGGCACCGCCGCCGGCCAGATGCTGTCCGGCCTGGCGCTGGCCTGCCTGCTGGTGGCCGCGGTTGCG
GTGTGGCAGCGCGCGCTGCGCTGA

Upstream 100 bases:

>100_bases
TTATTCAATGCTACGCACAATGTAAGTTGCGTGCATGTCGCCTCCGCCCCCGTCCTTTCCGCCCCCCGCCGTCCCCTGCA
CGGCCGCTCCCGCCCGTGCG

Downstream 100 bases:

>100_bases
GGGCGTCTCGCGCGCGGTTGTGTGCGCGCGGCGGCGATGGCCTAATCGCGGATTCGATCGGAATGGCGGGGCGGTGATGA
CGGCGGCAAGCAGCAAGGTG

Product: multidrug resistence transmembrane protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MLLSAHPELRPLRVHAFGATLGLVAGVDFVAAILIAMAGTQVQHGLGVAPRDFLWALTSYAVAAVMANLMLVQLAGRVNY
RRFTLASLAVFIAGALGCAAADSLPAFTAARLVQGLGGGGLFAAARILAQYSSQPQERLSLFWGFGVANFALVALAPWLA
GWLVAHHGWHTLFLLQAGLALPLLPLVAWLYPRVERTPSRALGRLDWPGVLAFAGGALLLVHALEHLRYLSRAELPQLAA
YALLGLVLLGVVMRRFARHPDPWLDPRRLASRRYLAGLAFYGVFYLICGYWNFLVPAVLVDGLGFGLDTVGGLLALGGLT
TLGAVVVFHLCLPYIMRKRRYIALGYVGLAAAFTLMAVSARPGAGVLDVLPPIVLQGAMPVLALLQVAMMTFVDMPTEDF
AHAYAFKNIVREILNALGTGLAVLQLHHGTAAAQAAMQAATPGPLQPHLAEVVHRAAVGTAAGQMLSGLALACLLVAAVA
VWQRALR

Sequences:

>Translated_487_residues
MLLSAHPELRPLRVHAFGATLGLVAGVDFVAAILIAMAGTQVQHGLGVAPRDFLWALTSYAVAAVMANLMLVQLAGRVNY
RRFTLASLAVFIAGALGCAAADSLPAFTAARLVQGLGGGGLFAAARILAQYSSQPQERLSLFWGFGVANFALVALAPWLA
GWLVAHHGWHTLFLLQAGLALPLLPLVAWLYPRVERTPSRALGRLDWPGVLAFAGGALLLVHALEHLRYLSRAELPQLAA
YALLGLVLLGVVMRRFARHPDPWLDPRRLASRRYLAGLAFYGVFYLICGYWNFLVPAVLVDGLGFGLDTVGGLLALGGLT
TLGAVVVFHLCLPYIMRKRRYIALGYVGLAAAFTLMAVSARPGAGVLDVLPPIVLQGAMPVLALLQVAMMTFVDMPTEDF
AHAYAFKNIVREILNALGTGLAVLQLHHGTAAAQAAMQAATPGPLQPHLAEVVHRAAVGTAAGQMLSGLALACLLVAAVA
VWQRALR
>Mature_487_residues
MLLSAHPELRPLRVHAFGATLGLVAGVDFVAAILIAMAGTQVQHGLGVAPRDFLWALTSYAVAAVMANLMLVQLAGRVNY
RRFTLASLAVFIAGALGCAAADSLPAFTAARLVQGLGGGGLFAAARILAQYSSQPQERLSLFWGFGVANFALVALAPWLA
GWLVAHHGWHTLFLLQAGLALPLLPLVAWLYPRVERTPSRALGRLDWPGVLAFAGGALLLVHALEHLRYLSRAELPQLAA
YALLGLVLLGVVMRRFARHPDPWLDPRRLASRRYLAGLAFYGVFYLICGYWNFLVPAVLVDGLGFGLDTVGGLLALGGLT
TLGAVVVFHLCLPYIMRKRRYIALGYVGLAAAFTLMAVSARPGAGVLDVLPPIVLQGAMPVLALLQVAMMTFVDMPTEDF
AHAYAFKNIVREILNALGTGLAVLQLHHGTAAAQAAMQAATPGPLQPHLAEVVHRAAVGTAAGQMLSGLALACLLVAAVA
VWQRALR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=25.7281553398058, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1788710, Length=408, Percent_Identity=22.5490196078431, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI87081983, Length=254, Percent_Identity=27.9527559055118, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51672; Mature: 51672

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSAHPELRPLRVHAFGATLGLVAGVDFVAAILIAMAGTQVQHGLGVAPRDFLWALTSY
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHH
AVAAVMANLMLVQLAGRVNYRRFTLASLAVFIAGALGCAAADSLPAFTAARLVQGLGGGG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
LFAAARILAQYSSQPQERLSLFWGFGVANFALVALAPWLAGWLVAHHGWHTLFLLQAGLA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LPLLPLVAWLYPRVERTPSRALGRLDWPGVLAFAGGALLLVHALEHLRYLSRAELPQLAA
HHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
YALLGLVLLGVVMRRFARHPDPWLDPRRLASRRYLAGLAFYGVFYLICGYWNFLVPAVLV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGLGFGLDTVGGLLALGGLTTLGAVVVFHLCLPYIMRKRRYIALGYVGLAAAFTLMAVSA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
RPGAGVLDVLPPIVLQGAMPVLALLQVAMMTFVDMPTEDFAHAYAFKNIVREILNALGTG
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCH
LAVLQLHHGTAAAQAAMQAATPGPLQPHLAEVVHRAAVGTAAGQMLSGLALACLLVAAVA
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWQRALR
HHHHHCC
>Mature Secondary Structure
MLLSAHPELRPLRVHAFGATLGLVAGVDFVAAILIAMAGTQVQHGLGVAPRDFLWALTSY
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHH
AVAAVMANLMLVQLAGRVNYRRFTLASLAVFIAGALGCAAADSLPAFTAARLVQGLGGGG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCH
LFAAARILAQYSSQPQERLSLFWGFGVANFALVALAPWLAGWLVAHHGWHTLFLLQAGLA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LPLLPLVAWLYPRVERTPSRALGRLDWPGVLAFAGGALLLVHALEHLRYLSRAELPQLAA
HHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
YALLGLVLLGVVMRRFARHPDPWLDPRRLASRRYLAGLAFYGVFYLICGYWNFLVPAVLV
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DGLGFGLDTVGGLLALGGLTTLGAVVVFHLCLPYIMRKRRYIALGYVGLAAAFTLMAVSA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
RPGAGVLDVLPPIVLQGAMPVLALLQVAMMTFVDMPTEDFAHAYAFKNIVREILNALGTG
CCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCH
LAVLQLHHGTAAAQAAMQAATPGPLQPHLAEVVHRAAVGTAAGQMLSGLALACLLVAAVA
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VWQRALR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]