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The map label for this gene is cca

Identifier: 119896784

GI number: 119896784

Start: 528605

End: 529843

Strand: Reverse

Name: cca

Synonym: azo0493

Alternate gene names: 119896784

Gene position: 529843-528605 (Counterclockwise)

Preceding gene: 119896785

Following gene: 119896782

Centisome position: 12.11

GC content: 72.32

Gene sequence:

>1239_bases
ATGCGCACCTATGTCGTCGGCGGCGCGGTGCGCGACGCCCTGCTCGGCCTGCCGGTAAAGGACCGTGACTGGGTGGTGGT
CGGCGCCACGCCAGACGAGATGCTGGCGCGCGGCTTCCGCCCGGTGGGCAAGGACTTTCCGGTATTCCTGCATCCGCAGA
CACAGGAAGAGCATGCGCTGGCGCGCACCGAGCGCAAGACCGGCCGCGGCTACGCGGGCTTCGCCTTCCACACCGCGCCC
GACGTGACGCTGGAAGAGGATCTCGCCCGCCGCGACCTCACGATCAACGCGATCGCGCGCGACGCGGACGGCACGCTGAT
CGACCCCTATGGCGGCGTGGCCGACCTGCACGGCCGGGTGTTCCGCCACGTCAGCCCCGCCTTCGCCGAAGACCCGGTGC
GCATCCTGCGCGTCGCCCGGTTCGCGGCGCGCTTTGCCGATTTTTCGGTGGCCCCGGAAACCCTGGCGTTGATGCGCGCG
ATGGTGGACAACGGCGAGGTGGACCACCTCGTCGCGGAGCGCGTGTGGCAGGAGTTCGCGCGCGGGCTGATGGAGGCGCA
CCCGGCGCGCATGATCCGCGTGTTGCGCGACTGCGGCGCGCTCGCGCGCCTGCTGCCGGAACTCGACCGCCTGTTCGGTG
TGCCGCAGCCGGCGCAGCATCACCCCGAAATCGACACCGGCGAGCATGTGCTGATGGTCGTCGAGCAGGCTGCGGCGCGC
GCACACAGCCTGCCGGTGCGGTGGGCAACCCTGCTGCACGACCTCGGCAAGGGCGAAACGCCGGCGGCGATCCTGCCCCA
CCACTACGGCCACGAAGCGCGCAGCGCAGACCTCGCGCGCCAGGTGTCGGAGCGGCTGAAAGCGCCGGTCGATTGCCGCG
ACCTCGCGGTGATGGTGGCGCGCGAACACGGCATCCTCGCCCAGGCAGCCGTGTTGCGGGCGGAGACGATGGTGAAGGTG
CTGGAACGCTGCGATGCCCTGCGGCGCCCGGAACGTTTTGCGCTGATGCTGGAGGCAGCCGCCTGCGACCATCTCGGCCG
CGGCGGCGAGCGCCCGGAGAACTGGGCGCCGGCGGCGCAATGGCAGGCCGCGCTTGCCGCGGTGCGCAGCGTGGAAGCGG
GTGCGATTGCGCGCGCCTGCGCCGACAAGGCCCAGGTGCCGCAGCGCATCCACGCCGCGCGCGTCGCGGCGGTCAAGGCC
CTGCGCGCCCACCCACCGCCGGCCGAGGGGATCTCGTGA

Upstream 100 bases:

>100_bases
CCTACCTCGCCACCATGCTCGCCCACCCCGACATGCGCGCGTGGCAGGCGGGCGCGCTGGCGGAAACCGAGGCCATCCCC
GGCGAGGACCTCTACGGCTG

Downstream 100 bases:

>100_bases
CGGCTGCCCGCCGGATCAAAGGGCGCGCAGCAGCAGCCACGCCAGCAGGGACAACAGCAGCGTGGTGGCGAATGGAAAGC
GATAGCTGCGCCCGCGCACG

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MRTYVVGGAVRDALLGLPVKDRDWVVVGATPDEMLARGFRPVGKDFPVFLHPQTQEEHALARTERKTGRGYAGFAFHTAP
DVTLEEDLARRDLTINAIARDADGTLIDPYGGVADLHGRVFRHVSPAFAEDPVRILRVARFAARFADFSVAPETLALMRA
MVDNGEVDHLVAERVWQEFARGLMEAHPARMIRVLRDCGALARLLPELDRLFGVPQPAQHHPEIDTGEHVLMVVEQAAAR
AHSLPVRWATLLHDLGKGETPAAILPHHYGHEARSADLARQVSERLKAPVDCRDLAVMVAREHGILAQAAVLRAETMVKV
LERCDALRRPERFALMLEAAACDHLGRGGERPENWAPAAQWQAALAAVRSVEAGAIARACADKAQVPQRIHAARVAAVKA
LRAHPPPAEGIS

Sequences:

>Translated_412_residues
MRTYVVGGAVRDALLGLPVKDRDWVVVGATPDEMLARGFRPVGKDFPVFLHPQTQEEHALARTERKTGRGYAGFAFHTAP
DVTLEEDLARRDLTINAIARDADGTLIDPYGGVADLHGRVFRHVSPAFAEDPVRILRVARFAARFADFSVAPETLALMRA
MVDNGEVDHLVAERVWQEFARGLMEAHPARMIRVLRDCGALARLLPELDRLFGVPQPAQHHPEIDTGEHVLMVVEQAAAR
AHSLPVRWATLLHDLGKGETPAAILPHHYGHEARSADLARQVSERLKAPVDCRDLAVMVAREHGILAQAAVLRAETMVKV
LERCDALRRPERFALMLEAAACDHLGRGGERPENWAPAAQWQAALAAVRSVEAGAIARACADKAQVPQRIHAARVAAVKA
LRAHPPPAEGIS
>Mature_412_residues
MRTYVVGGAVRDALLGLPVKDRDWVVVGATPDEMLARGFRPVGKDFPVFLHPQTQEEHALARTERKTGRGYAGFAFHTAP
DVTLEEDLARRDLTINAIARDADGTLIDPYGGVADLHGRVFRHVSPAFAEDPVRILRVARFAARFADFSVAPETLALMRA
MVDNGEVDHLVAERVWQEFARGLMEAHPARMIRVLRDCGALARLLPELDRLFGVPQPAQHHPEIDTGEHVLMVVEQAAAR
AHSLPVRWATLLHDLGKGETPAAILPHHYGHEARSADLARQVSERLKAPVDCRDLAVMVAREHGILAQAAVLRAETMVKV
LERCDALRRPERFALMLEAAACDHLGRGGERPENWAPAAQWQAALAAVRSVEAGAIARACADKAQVPQRIHAARVAAVKA
LRAHPPPAEGIS

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Homo sapiens, GI155030240, Length=209, Percent_Identity=32.5358851674641, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789436, Length=410, Percent_Identity=51.9512195121951, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI87081691, Length=225, Percent_Identity=32, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_AZOSB (A1K2Q5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931997.1
- ProteinModelPortal:   A1K2Q5
- SMR:   A1K2Q5
- STRING:   A1K2Q5
- GeneID:   4606430
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0493
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   RWACLLH
- PhylomeDB:   A1K2Q5
- ProtClustDB:   PRK10885
- BioCyc:   ASP62928:AZO0493-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45080; Mature: 45080

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTYVVGGAVRDALLGLPVKDRDWVVVGATPDEMLARGFRPVGKDFPVFLHPQTQEEHAL
CCEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTGRGYAGFAFHTAPDVTLEEDLARRDLTINAIARDADGTLIDPYGGVADLHGRV
HHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCHHHEEECCCCCEECCCCCHHHHHHHH
FRHVSPAFAEDPVRILRVARFAARFADFSVAPETLALMRAMVDNGEVDHLVAERVWQEFA
HHHCCCHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGLMEAHPARMIRVLRDCGALARLLPELDRLFGVPQPAQHHPEIDTGEHVLMVVEQAAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AHSLPVRWATLLHDLGKGETPAAILPHHYGHEARSADLARQVSERLKAPVDCRDLAVMVA
HHCCCHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
REHGILAQAAVLRAETMVKVLERCDALRRPERFALMLEAAACDHLGRGGERPENWAPAAQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
WQAALAAVRSVEAGAIARACADKAQVPQRIHAARVAAVKALRAHPPPAEGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRTYVVGGAVRDALLGLPVKDRDWVVVGATPDEMLARGFRPVGKDFPVFLHPQTQEEHAL
CCEEEECHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCCCCCCEEECCCCCHHHHH
ARTERKTGRGYAGFAFHTAPDVTLEEDLARRDLTINAIARDADGTLIDPYGGVADLHGRV
HHHHHHCCCCCCCEEECCCCCCCHHHHHHHHCCCHHHEEECCCCCEECCCCCHHHHHHHH
FRHVSPAFAEDPVRILRVARFAARFADFSVAPETLALMRAMVDNGEVDHLVAERVWQEFA
HHHCCCHHHHCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGLMEAHPARMIRVLRDCGALARLLPELDRLFGVPQPAQHHPEIDTGEHVLMVVEQAAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AHSLPVRWATLLHDLGKGETPAAILPHHYGHEARSADLARQVSERLKAPVDCRDLAVMVA
HHCCCHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
REHGILAQAAVLRAETMVKVLERCDALRRPERFALMLEAAACDHLGRGGERPENWAPAAQ
HHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
WQAALAAVRSVEAGAIARACADKAQVPQRIHAARVAAVKALRAHPPPAEGIS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA