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The map label for this gene is isoJ [H]

Identifier: 119896782

GI number: 119896782

Start: 527690

End: 528310

Strand: Reverse

Name: isoJ [H]

Synonym: azo0491

Alternate gene names: 119896782

Gene position: 528310-527690 (Counterclockwise)

Preceding gene: 119896784

Following gene: 119896781

Centisome position: 12.07

GC content: 65.54

Gene sequence:

>621_bases
ATGATCACGCTTTATACCTGGGGTACGCCCAACGGACGCAAGATCTCGATCGCGCTCGAAGAACTGGGACTGGACTACAC
CGTCCGGCCGATCGACATCACCCGCGGCGAGCAGCACGAGGCGGGCTTCGTCGCGCTCAGCCCGAACAACAAGATTCCGG
TGCTGGTCGATGACGAGGGCCCGGGCGGTGCGCCGATCACGCTGATCGAATCCGGCGCGATCCTGATCTACCTCGCCGAG
AAGCACGGCCAGCTGCTGTCGGCCGAGCCACGCGAGCGGCTGGAAACCCTGCAATGGCTGATGCTGCAGATGGGAAGCAT
CGGCCCGATGCTGGGCCAGGCGCACCACTTCCTGCGTTTTGCGCCGGACGTCATTCCGTACGCGATCGACCGCTACTCGA
AGGAAGCGGTGCGTCTGTACGGCGTCCTCAACACCCGCCTCGCGGGGCGCGACTGGCTTGCCGGCGCAGCCTATTCGATC
GCCGACATCGCCACCTGGCCATGGATAGACCGGCACAAATGGCAGGGCATCGACCTCGCCCGCTACCCGGAGGTCAAGCG
CTGGTATGAAACCATCGCAGCGCGCCCCGCGGTGCAGCGCGGCATGGAGGTGCCGCGATGA

Upstream 100 bases:

>100_bases
CCACGAACCACTTCAGCATCCCACGCTCCATACAAGAACCGGACCTTCCGGACCCACCCCAACGACCCCGCAGCGGGCAT
CCGACAGAGCCAGTACACGC

Downstream 100 bases:

>100_bases
ACAGCACCCGTTTCGGAGAACTGGAAGTCATCTGCCACGCCCCGGCCGGCACGCCGGCCTTTGAAACGCCGCTGCTGTTC
ATCCACGGCGCCTATGTCGG

Product: glutathione S-transferase

Products: NA

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MITLYTWGTPNGRKISIALEELGLDYTVRPIDITRGEQHEAGFVALSPNNKIPVLVDDEGPGGAPITLIESGAILIYLAE
KHGQLLSAEPRERLETLQWLMLQMGSIGPMLGQAHHFLRFAPDVIPYAIDRYSKEAVRLYGVLNTRLAGRDWLAGAAYSI
ADIATWPWIDRHKWQGIDLARYPEVKRWYETIAARPAVQRGMEVPR

Sequences:

>Translated_206_residues
MITLYTWGTPNGRKISIALEELGLDYTVRPIDITRGEQHEAGFVALSPNNKIPVLVDDEGPGGAPITLIESGAILIYLAE
KHGQLLSAEPRERLETLQWLMLQMGSIGPMLGQAHHFLRFAPDVIPYAIDRYSKEAVRLYGVLNTRLAGRDWLAGAAYSI
ADIATWPWIDRHKWQGIDLARYPEVKRWYETIAARPAVQRGMEVPR
>Mature_206_residues
MITLYTWGTPNGRKISIALEELGLDYTVRPIDITRGEQHEAGFVALSPNNKIPVLVDDEGPGGAPITLIESGAILIYLAE
KHGQLLSAEPRERLETLQWLMLQMGSIGPMLGQAHHFLRFAPDVIPYAIDRYSKEAVRLYGVLNTRLAGRDWLAGAAYSI
ADIATWPWIDRHKWQGIDLARYPEVKRWYETIAARPAVQRGMEVPR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=203, Percent_Identity=55.6650246305419, Blast_Score=220, Evalue=7e-59,
Organism=Escherichia coli, GI87082195, Length=218, Percent_Identity=41.743119266055, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI87081789, Length=178, Percent_Identity=33.1460674157303, Blast_Score=85, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324100, Length=240, Percent_Identity=31.25, Blast_Score=112, Evalue=5e-26,
Organism=Drosophila melanogaster, GI28571670, Length=174, Percent_Identity=31.6091954022989, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI17933730, Length=188, Percent_Identity=30.8510638297872, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17864598, Length=183, Percent_Identity=31.6939890710383, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17864592, Length=196, Percent_Identity=29.0816326530612, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864596, Length=187, Percent_Identity=27.807486631016, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17864594, Length=193, Percent_Identity=29.5336787564767, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI85725204, Length=196, Percent_Identity=28.0612244897959, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17737923, Length=196, Percent_Identity=28.0612244897959, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646249, Length=198, Percent_Identity=29.7979797979798, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24646251, Length=182, Percent_Identity=30.2197802197802, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI45549270, Length=182, Percent_Identity=27.4725274725275, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922932, Length=209, Percent_Identity=28.2296650717703, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922160, Length=214, Percent_Identity=24.2990654205607, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 23171; Mature: 23171

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLYTWGTPNGRKISIALEELGLDYTVRPIDITRGEQHEAGFVALSPNNKIPVLVDDEG
CEEEEECCCCCCCEEEEEHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
PGGAPITLIESGAILIYLAEKHGQLLSAEPRERLETLQWLMLQMGSIGPMLGQAHHFLRF
CCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
APDVIPYAIDRYSKEAVRLYGVLNTRLAGRDWLAGAAYSIADIATWPWIDRHKWQGIDLA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHCCCCCCCCCCCCCCCCH
RYPEVKRWYETIAARPAVQRGMEVPR
HCCHHHHHHHHHHCCHHHHCCCCCCC
>Mature Secondary Structure
MITLYTWGTPNGRKISIALEELGLDYTVRPIDITRGEQHEAGFVALSPNNKIPVLVDDEG
CEEEEECCCCCCCEEEEEHHHCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCEEEECCC
PGGAPITLIESGAILIYLAEKHGQLLSAEPRERLETLQWLMLQMGSIGPMLGQAHHFLRF
CCCCEEEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
APDVIPYAIDRYSKEAVRLYGVLNTRLAGRDWLAGAAYSIADIATWPWIDRHKWQGIDLA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHCCCCCCCCCCCCCCCCH
RYPEVKRWYETIAARPAVQRGMEVPR
HCCHHHHHHHHHHCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]