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The map label for this gene is mdeA [H]

Identifier: 119896763

GI number: 119896763

Start: 509624

End: 510913

Strand: Direct

Name: mdeA [H]

Synonym: azo0472

Alternate gene names: 119896763

Gene position: 509624-510913 (Clockwise)

Preceding gene: 119896761

Following gene: 119896764

Centisome position: 11.65

GC content: 69.15

Gene sequence:

>1290_bases
ATGCCCGATCATCGCTTCGGCATCGAATCCCTTTGCCTGCACGCCGGCCAGCAGCCCGACGCCGAAACCGGCGCGCGCGC
GGTGCCGATCTACCAGACCACCTCCTTCGTCTTCGATTCGCCCGAGCACGCGGCCGAACTGTTCAACCTGCAGACCTTCG
GCAACGTCTATTCGCGCATCTCCAACCCCACGGTGGCGGTGTTCGAAGAGCGCATGGCGGCGCTCGAAGGCGGCCGCGCC
GCGCTCGCCACCTCGTCCGGCCTGGCCGCGCAGATGACCGCGCTGCTGACCCTGGCGCAGGCGGGCGACGAAATCGTCGC
GGCGCGCACGCTGTACGGCGGCAGCTATTCCCAGCTCGACGTCAGCCTGCGCCGGCTCGGCATCAGCACGGTGTTCGTCG
ATCCGTCCGACCCGGAGAACTTCCGCGCCGCGATCACCGACCGCACCAAGGCGGTGTATGGCGAGATCATCGGCAACCCC
AGCCTCAACGTGTTCGACGTGGCCGCGGTGGCGGCGATCACCCGGGCTGCTGGGGTGCCGCTGGTGATCGACAGCACGCT
CGCCTCGCCCTACCTGTGCCGGCCGCTTGAACACGGCGCCGACATCGTCATCCACTCGGCCACCAAGTACATCGGCGGCC
ACGGCACCACCATGGGCGGCGTGCTGGTCGAATCCGGCACCTTCCCGTGGGACAACGGCCGCTTCCCGCAGATGGTCGAA
CCGTCGCCGGGCTACCACGGCGTGCGTTTCTACGAAACCTTCGGCAACTTCGGCTTCACGATGAAGGCGCGCATGGAAAC
CCTGCGCACCTTCGGCCAGGCGCTGTCGCCGTTCAACGCCTTCATGCTGCTGCAGGGGCTGGAGACGCTGCATGTGCGCA
TGGACCGCCATGTCGCCAATGCGCGCGCGGTGGCGGACTTCCTCGCCGCCCACCCGGCGGTGGCGTGGGTGAATTACCCC
GGCCTCGCCGCCAGCCCCGAGCACGCGCTGGCGCAGCGCTATTTCCCCAAGGGCCCGGGCGCCATCCTGTCCTTCGGCAT
CCGCGGCGGCCAGCCCGCCGGCGAGCGCTTCATCGAGGCGCTGCAGATGATCAGTCACCTCGCCAACGTCGGCGACGCCA
AGACCCTGGTGATCCACCCGGCCTCGACCACCCACCGCCAGCTCTCCGAAGAAGAGCAGCGCGCCGCCGGTGTGCCGCCC
GACATGGTGCGGCTGTCGGTCGGCATCGAAACCCTGGACGACATCCTCTGGGATCTCGACCAGGCGCTCGCGCGCGGAGC
CGCGGCGTGA

Upstream 100 bases:

>100_bases
CAGCCAGAACAGCAGCAGCGCAAAATCGAAAGTCATCGGGATTTGGCGGGCGAGGGTGGGGCCAGTATTATCGGCGCAAT
TCCTCAGCGTCCCCGCGCCC

Downstream 100 bases:

>100_bases
GTCTGCGCCTGCTGCTCGGTATCGCGATCGCATTGCTGCTGGTCTACGGCGGCTGGCAGCTGCTGCGCGCGGTGCGCGGC
GGCTGGCGCGCGGGACGGGC

Product: O-acetylhomoserine aminocarboxypropyltransferase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MPDHRFGIESLCLHAGQQPDAETGARAVPIYQTTSFVFDSPEHAAELFNLQTFGNVYSRISNPTVAVFEERMAALEGGRA
ALATSSGLAAQMTALLTLAQAGDEIVAARTLYGGSYSQLDVSLRRLGISTVFVDPSDPENFRAAITDRTKAVYGEIIGNP
SLNVFDVAAVAAITRAAGVPLVIDSTLASPYLCRPLEHGADIVIHSATKYIGGHGTTMGGVLVESGTFPWDNGRFPQMVE
PSPGYHGVRFYETFGNFGFTMKARMETLRTFGQALSPFNAFMLLQGLETLHVRMDRHVANARAVADFLAAHPAVAWVNYP
GLAASPEHALAQRYFPKGPGAILSFGIRGGQPAGERFIEALQMISHLANVGDAKTLVIHPASTTHRQLSEEEQRAAGVPP
DMVRLSVGIETLDDILWDLDQALARGAAA

Sequences:

>Translated_429_residues
MPDHRFGIESLCLHAGQQPDAETGARAVPIYQTTSFVFDSPEHAAELFNLQTFGNVYSRISNPTVAVFEERMAALEGGRA
ALATSSGLAAQMTALLTLAQAGDEIVAARTLYGGSYSQLDVSLRRLGISTVFVDPSDPENFRAAITDRTKAVYGEIIGNP
SLNVFDVAAVAAITRAAGVPLVIDSTLASPYLCRPLEHGADIVIHSATKYIGGHGTTMGGVLVESGTFPWDNGRFPQMVE
PSPGYHGVRFYETFGNFGFTMKARMETLRTFGQALSPFNAFMLLQGLETLHVRMDRHVANARAVADFLAAHPAVAWVNYP
GLAASPEHALAQRYFPKGPGAILSFGIRGGQPAGERFIEALQMISHLANVGDAKTLVIHPASTTHRQLSEEEQRAAGVPP
DMVRLSVGIETLDDILWDLDQALARGAAA
>Mature_428_residues
PDHRFGIESLCLHAGQQPDAETGARAVPIYQTTSFVFDSPEHAAELFNLQTFGNVYSRISNPTVAVFEERMAALEGGRAA
LATSSGLAAQMTALLTLAQAGDEIVAARTLYGGSYSQLDVSLRRLGISTVFVDPSDPENFRAAITDRTKAVYGEIIGNPS
LNVFDVAAVAAITRAAGVPLVIDSTLASPYLCRPLEHGADIVIHSATKYIGGHGTTMGGVLVESGTFPWDNGRFPQMVEP
SPGYHGVRFYETFGNFGFTMKARMETLRTFGQALSPFNAFMLLQGLETLHVRMDRHVANARAVADFLAAHPAVAWVNYPG
LAASPEHALAQRYFPKGPGAILSFGIRGGQPAGERFIEALQMISHLANVGDAKTLVIHPASTTHRQLSEEEQRAAGVPPD
MVRLSVGIETLDDILWDLDQALARGAAA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=400, Percent_Identity=34.25, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI299473759, Length=400, Percent_Identity=31, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI25453487, Length=399, Percent_Identity=30.3258145363409, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI1790375, Length=412, Percent_Identity=31.3106796116505, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1789383, Length=439, Percent_Identity=26.879271070615, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71980708, Length=414, Percent_Identity=31.1594202898551, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17560056, Length=422, Percent_Identity=33.6492890995261, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=415, Percent_Identity=33.7349397590361, Blast_Score=198, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=48.1735159817352, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6319307, Length=421, Percent_Identity=34.4418052256532, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321254, Length=448, Percent_Identity=27.4553571428571, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6321170, Length=367, Percent_Identity=23.7057220708447, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321904, Length=427, Percent_Identity=25.5269320843091, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19922554, Length=430, Percent_Identity=33.2558139534884, Blast_Score=211, Evalue=6e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45976; Mature: 45844

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDHRFGIESLCLHAGQQPDAETGARAVPIYQTTSFVFDSPEHAAELFNLQTFGNVYSRI
CCCCHHCHHHHHHHCCCCCCCCCCCEEEEEEEECHHEECCHHHHHHHHHHHHHHHHHHHH
SNPTVAVFEERMAALEGGRAALATSSGLAAQMTALLTLAQAGDEIVAARTLYGGSYSQLD
CCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
VSLRRLGISTVFVDPSDPENFRAAITDRTKAVYGEIIGNPSLNVFDVAAVAAITRAAGVP
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
LVIDSTLASPYLCRPLEHGADIVIHSATKYIGGHGTTMGGVLVESGTFPWDNGRFPQMVE
EEEECCCCCCHHCCHHHCCCCEEEECCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
PSPGYHGVRFYETFGNFGFTMKARMETLRTFGQALSPFNAFMLLQGLETLHVRMDRHVAN
CCCCCCCEEHEEHHCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ARAVADFLAAHPAVAWVNYPGLAASPEHALAQRYFPKGPGAILSFGIRGGQPAGERFIEA
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHH
LQMISHLANVGDAKTLVIHPASTTHRQLSEEEQRAAGVPPDMVRLSVGIETLDDILWDLD
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHEEEECCHHHHHHHHHHHH
QALARGAAA
HHHHCCCCC
>Mature Secondary Structure 
PDHRFGIESLCLHAGQQPDAETGARAVPIYQTTSFVFDSPEHAAELFNLQTFGNVYSRI
CCCHHCHHHHHHHCCCCCCCCCCCEEEEEEEECHHEECCHHHHHHHHHHHHHHHHHHHH
SNPTVAVFEERMAALEGGRAALATSSGLAAQMTALLTLAQAGDEIVAARTLYGGSYSQLD
CCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHH
VSLRRLGISTVFVDPSDPENFRAAITDRTKAVYGEIIGNPSLNVFDVAAVAAITRAAGVP
HHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
LVIDSTLASPYLCRPLEHGADIVIHSATKYIGGHGTTMGGVLVESGTFPWDNGRFPQMVE
EEEECCCCCCHHCCHHHCCCCEEEECCHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCC
PSPGYHGVRFYETFGNFGFTMKARMETLRTFGQALSPFNAFMLLQGLETLHVRMDRHVAN
CCCCCCCEEHEEHHCCCCEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
ARAVADFLAAHPAVAWVNYPGLAASPEHALAQRYFPKGPGAILSFGIRGGQPAGERFIEA
HHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHH
LQMISHLANVGDAKTLVIHPASTTHRQLSEEEQRAAGVPPDMVRLSVGIETLDDILWDLD
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHEEEECCHHHHHHHHHHHH
QALARGAAA
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]