BacMap

Definition	Azoarcus sp. BH72 chromosome, complete genome.
Accession	NC_008702
Length	4,376,040

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The map label for this gene is paaG2 [H]

Identifier: 119896761

GI number: 119896761

Start: 507945

End: 508745

Strand: Direct

Name: paaG2 [H]

Synonym: azo0470

Alternate gene names: 119896761

Gene position: 507945-508745 (Clockwise)

Preceding gene: 119896760

Following gene: 119896763

Centisome position: 11.61

GC content: 69.16

Gene sequence:

>801_bases
ATGGCCGATACCGTATTGCTCGACGTCGCCGATGGCGTCGCCACCCTGACGCTGAACCGCCCGGACGTGCTCAACGTATT
GTCGGTGGCGATGATGCAGGACCTTGCCGAAGCGGTCCGCGCGCTTGCCGCGCGCAAGGACGTGGCGGTGGTGGTCATCA
CCGGCGCCGGCGCGCATTTCATGGCCGGCGGCGACCTGAAGGACTTCTCCGAGCACCTGCACCTGTCGTCCGAGGCGCGC
CTGGCGACCTTCCGCGCGATGATCGCGCAGCACATCAATCCCACCGTGGAAACCCTGCAGGGCCTGCCGCAGCCGGTGAT
CGCCAAGGTGCGCGGCGCCTGCGCCGGCTTCGGGCTGTCGCTGATGCTGGGCTGCGACTTCGCGCTGTGCGCCGACAACA
GCGTGTTCACCACCGCCTATTCGGCGATCGGCCTGCCTGGCGATGGCGGCGCATCCTACTTCCTGCCGCGCCTGGTCGGT
CGCCGCAAGGCCGCCGAGCTGCTGCTGCTGGCCGAGCGCTTCGACACCGCCGAAGCCCTGCGCCTGGGCCTGATCAACCG
CGCCGTGCCGGAGGCCGAACTGGATGCGGTGACCGACCAGTTCGTCGCCCGGCTCAAGGCCGGTCCGCGCCACGCCTATG
CCGAGATCAAGCGCCTGCTCGCCGGCTCGCACGACAACCAGCTCGAATCGCAATTGCAGAACGAGGCCGAAGCCTTCGGC
CGCTGCGGTGCCACCGCCGACTTCGCCGAAGGCGTCACCGCCTTCCTCGGCAAGCGCAAGCCGGGCTTCAAGGGGGTCTG
A

Upstream 100 bases:

>100_bases
GCCGCGATCTTCGAACGCGTATAGGCGGCGCGGGCCGCTAAACTTCCGGCTCTGGGCGGCGCCGCGGGTGCGCCGCCGCA
ACCCCCTGGAGCCCCCCGAG

Downstream 100 bases:

>100_bases
GCCGCCGCGCCGCCGGCCGGCTTGCTGCTCAGCGGCTCAGCCGGTAGGCGAGCAGGCCCATCCATTCGTGCAGCGCGTAC
CAGCCGGCGTAGGCCGTGCC

Product: putative enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MADTVLLDVADGVATLTLNRPDVLNVLSVAMMQDLAEAVRALAARKDVAVVVITGAGAHFMAGGDLKDFSEHLHLSSEAR
LATFRAMIAQHINPTVETLQGLPQPVIAKVRGACAGFGLSLMLGCDFALCADNSVFTTAYSAIGLPGDGGASYFLPRLVG
RRKAAELLLLAERFDTAEALRLGLINRAVPEAELDAVTDQFVARLKAGPRHAYAEIKRLLAGSHDNQLESQLQNEAEAFG
RCGATADFAEGVTAFLGKRKPGFKGV

Sequences:

>Translated_266_residues
MADTVLLDVADGVATLTLNRPDVLNVLSVAMMQDLAEAVRALAARKDVAVVVITGAGAHFMAGGDLKDFSEHLHLSSEAR
LATFRAMIAQHINPTVETLQGLPQPVIAKVRGACAGFGLSLMLGCDFALCADNSVFTTAYSAIGLPGDGGASYFLPRLVG
RRKAAELLLLAERFDTAEALRLGLINRAVPEAELDAVTDQFVARLKAGPRHAYAEIKRLLAGSHDNQLESQLQNEAEAFG
RCGATADFAEGVTAFLGKRKPGFKGV
>Mature_265_residues
ADTVLLDVADGVATLTLNRPDVLNVLSVAMMQDLAEAVRALAARKDVAVVVITGAGAHFMAGGDLKDFSEHLHLSSEARL
ATFRAMIAQHINPTVETLQGLPQPVIAKVRGACAGFGLSLMLGCDFALCADNSVFTTAYSAIGLPGDGGASYFLPRLVGR
RKAAELLLLAERFDTAEALRLGLINRAVPEAELDAVTDQFVARLKAGPRHAYAEIKRLLAGSHDNQLESQLQNEAEAFGR
CGATADFAEGVTAFLGKRKPGFKGV

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=257, Percent_Identity=31.1284046692607, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=32.258064516129, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI45643119, Length=205, Percent_Identity=25.3658536585366, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260275230, Length=205, Percent_Identity=25.3658536585366, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI260274832, Length=205, Percent_Identity=25.3658536585366, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=29.0196078431373, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI37594469, Length=161, Percent_Identity=31.055900621118, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI37594471, Length=161, Percent_Identity=31.055900621118, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI25453481, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4757966, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI51538573, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI51538581, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4757968, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=184, Percent_Identity=25.5434782608696, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694516, Length=233, Percent_Identity=24.8927038626609, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI213417737, Length=233, Percent_Identity=24.8927038626609, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI68989263, Length=180, Percent_Identity=29.4444444444444, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=30.7692307692308, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136756, Length=239, Percent_Identity=24.2677824267782, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=239, Percent_Identity=24.2677824267782, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=239, Percent_Identity=24.2677824267782, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=265, Percent_Identity=34.7169811320755, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=29.9610894941634, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=31.496062992126, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI221142681, Length=279, Percent_Identity=29.3906810035842, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=26.7175572519084, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788682, Length=189, Percent_Identity=29.1005291005291, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790281, Length=264, Percent_Identity=28.4090909090909, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=30.9803921568627, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17535521, Length=249, Percent_Identity=25.7028112449799, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=201, Percent_Identity=30.3482587064677, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=262, Percent_Identity=29.0076335877863, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=185, Percent_Identity=28.6486486486486, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=194, Percent_Identity=28.3505154639175, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=183, Percent_Identity=31.1475409836066, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=268, Percent_Identity=27.6119402985075, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=26.9230769230769, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560910, Length=185, Percent_Identity=27.5675675675676, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=231, Percent_Identity=25.1082251082251, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922422, Length=271, Percent_Identity=30.6273062730627, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24654903, Length=224, Percent_Identity=29.9107142857143, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20129971, Length=252, Percent_Identity=28.968253968254, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24653477, Length=252, Percent_Identity=28.968253968254, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19920382, Length=253, Percent_Identity=29.2490118577075, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=28.4090909090909, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650670, Length=188, Percent_Identity=29.2553191489362, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=187, Percent_Identity=24.0641711229947, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28116; Mature: 27984

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTVLLDVADGVATLTLNRPDVLNVLSVAMMQDLAEAVRALAARKDVAVVVITGAGAHF
CCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCE
MAGGDLKDFSEHLHLSSEARLATFRAMIAQHINPTVETLQGLPQPVIAKVRGACAGFGLS
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LMLGCDFALCADNSVFTTAYSAIGLPGDGGASYFLPRLVGRRKAAELLLLAERFDTAEAL
HHHHCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
RLGLINRAVPEAELDAVTDQFVARLKAGPRHAYAEIKRLLAGSHDNQLESQLQNEAEAFG
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RCGATADFAEGVTAFLGKRKPGFKGV
CCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ADTVLLDVADGVATLTLNRPDVLNVLSVAMMQDLAEAVRALAARKDVAVVVITGAGAHF
CCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCE
MAGGDLKDFSEHLHLSSEARLATFRAMIAQHINPTVETLQGLPQPVIAKVRGACAGFGLS
ECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LMLGCDFALCADNSVFTTAYSAIGLPGDGGASYFLPRLVGRRKAAELLLLAERFDTAEAL
HHHHCCEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
RLGLINRAVPEAELDAVTDQFVARLKAGPRHAYAEIKRLLAGSHDNQLESQLQNEAEAFG
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RCGATADFAEGVTAFLGKRKPGFKGV
CCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1743516 [H]