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The map label for this gene is ansB2 [H]

Identifier: 119896729

GI number: 119896729

Start: 463964

End: 465061

Strand: Reverse

Name: ansB2 [H]

Synonym: azo0438

Alternate gene names: 119896729

Gene position: 465061-463964 (Counterclockwise)

Preceding gene: 119896730

Following gene: 119896728

Centisome position: 10.63

GC content: 69.85

Gene sequence:

>1098_bases
ATGCCCCTCTCCTCCCAACTCCGCCACTTGCTCCTCCCTGCCCTCGCCGCGGCGGCCTTGCTGCTGCCGCCCGCCGCCCA
CGCGCGTGAAGCCGCAGCGCCGGCACGCCTCGCCAAGGTGGTGATCCTCGCCACCGGCGGCACGATTGCCGGCGCCGGCG
CCGACGCGGCCTCCAGCGCCACCTACCAGGCCGCCAAGGTTCCGGTCGAAAAACTCGTCGCCGGCATCCCGGAGCTGAAG
AACCTTGCCGAAGTGCGCGGCGAGCAGGTGTTCCAGATCGCCTCCGAGAGCTTCACCAACGAGCACCTGCTGCAGCTCGG
CCGCCGGGTCGCGGAGCTGAGCGCCAGCGCGGATGTGGACGGCATCGTGATCACCCATGGCACCGATACGCTGGAGGAGA
CGGCGTTCTTCCTCAACCTGGTGATCCGCAGCGACAAGCCGGTGGTGGTGGTCGGTTCGATGCGGCCGGGCACCGCGCTG
TCGGCCGACGGCGCGCTCAACCTCTACGACGCGGTGGCGGTGGCGGCGAGCAGCGACGCGCGCGGCAAGGGCGTGCTGGT
GACGATGAACGACGAGATCCACAGCGGCCGCGACGTCAGCAAGGCGGTGAACATCCGCCCGGACGCGTTCCGCAGTCCGT
GGGGTCCGCTGGGCATGGTGGTGGAAGGCAAGCCCTACTGGTTCCGCCTGCCGGCCAAGCGCCACACGCTGAATTCCGAA
TTCGACATCGCCCGCATCCAGTCGCTGGCGCCGGTCGAGATCGCCTACGGCTACGGCAACGTAAGCGACACCGCCTACAA
GGCCTTCGCCGCCGCGGGCGCCAAGGCCATCATCCATGCCGGTACCGGCAACGGCTCGGTGTCGAACCGGGTGGTGCCGG
GGCTGCAGGCGCTGCGCGCGCAGGGCGTCCACATCGTGCGGTCGTCGCGGGTGGATGGCGGCGGCTTCGTGCTGCGCAAT
GCCGAACAGCCGGACGACAAGTACGACTGGGTCGCCGCGCACGACCTCAACCCGCAGAAGGCCCGCATCCTCGCCGCGGT
GGCGCTGACCACCACCAGCGACAGCCGCGAGCTGCAGCGCATCTTCTGGGAATACTGA

Upstream 100 bases:

>100_bases
GCCCCGTGCGGCCGCCAGAGCCGTGGCCTGACTTGCTCCCTCCCCTTCAAGGGGGAGGGACTACCCCCACCACATCGCGC
CAATACCCCCGGAGACCCAA

Downstream 100 bases:

>100_bases
GCACATGCCGCCCGGCGCCGCCCGGCGCCGTGGCAGCCGTCACACCGCTGCGCGCGGACGCGCCGCACCCTCTGCGCCGC
GCGCGGTCGAACCCGACGAG

Product: periplasmic L-asparaginase II

Products: NA

Alternate protein names: L-ASNase/L-GLNase; L-asparagine/L-glutamine amidohydrolase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MPLSSQLRHLLLPALAAAALLLPPAAHAREAAAPARLAKVVILATGGTIAGAGADAASSATYQAAKVPVEKLVAGIPELK
NLAEVRGEQVFQIASESFTNEHLLQLGRRVAELSASADVDGIVITHGTDTLEETAFFLNLVIRSDKPVVVVGSMRPGTAL
SADGALNLYDAVAVAASSDARGKGVLVTMNDEIHSGRDVSKAVNIRPDAFRSPWGPLGMVVEGKPYWFRLPAKRHTLNSE
FDIARIQSLAPVEIAYGYGNVSDTAYKAFAAAGAKAIIHAGTGNGSVSNRVVPGLQALRAQGVHIVRSSRVDGGGFVLRN
AEQPDDKYDWVAAHDLNPQKARILAAVALTTTSDSRELQRIFWEY

Sequences:

>Translated_365_residues
MPLSSQLRHLLLPALAAAALLLPPAAHAREAAAPARLAKVVILATGGTIAGAGADAASSATYQAAKVPVEKLVAGIPELK
NLAEVRGEQVFQIASESFTNEHLLQLGRRVAELSASADVDGIVITHGTDTLEETAFFLNLVIRSDKPVVVVGSMRPGTAL
SADGALNLYDAVAVAASSDARGKGVLVTMNDEIHSGRDVSKAVNIRPDAFRSPWGPLGMVVEGKPYWFRLPAKRHTLNSE
FDIARIQSLAPVEIAYGYGNVSDTAYKAFAAAGAKAIIHAGTGNGSVSNRVVPGLQALRAQGVHIVRSSRVDGGGFVLRN
AEQPDDKYDWVAAHDLNPQKARILAAVALTTTSDSRELQRIFWEY
>Mature_364_residues
PLSSQLRHLLLPALAAAALLLPPAAHAREAAAPARLAKVVILATGGTIAGAGADAASSATYQAAKVPVEKLVAGIPELKN
LAEVRGEQVFQIASESFTNEHLLQLGRRVAELSASADVDGIVITHGTDTLEETAFFLNLVIRSDKPVVVVGSMRPGTALS
ADGALNLYDAVAVAASSDARGKGVLVTMNDEIHSGRDVSKAVNIRPDAFRSPWGPLGMVVEGKPYWFRLPAKRHTLNSEF
DIARIQSLAPVEIAYGYGNVSDTAYKAFAAAGAKAIIHAGTGNGSVSNRVVPGLQALRAQGVHIVRSSRVDGGGFVLRNA
EQPDDKYDWVAAHDLNPQKARILAAVALTTTSDSRELQRIFWEY

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=312, Percent_Identity=44.8717948717949, Blast_Score=271, Evalue=4e-74,
Organism=Escherichia coli, GI1788065, Length=214, Percent_Identity=31.3084112149533, Blast_Score=68, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323189, Length=335, Percent_Identity=36.4179104477612, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323187, Length=335, Percent_Identity=36.4179104477612, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323186, Length=335, Percent_Identity=36.4179104477612, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323184, Length=335, Percent_Identity=36.4179104477612, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6320527, Length=345, Percent_Identity=33.6231884057971, Blast_Score=162, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004550
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.38 [H]

Molecular weight: Translated: 38548; Mature: 38417

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLSSQLRHLLLPALAAAALLLPPAAHAREAAAPARLAKVVILATGGTIAGAGADAASSA
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHEEEEEEEEECCEEECCCCCCCCCC
TYQAAKVPVEKLVAGIPELKNLAEVRGEQVFQIASESFTNEHLLQLGRRVAELSASADVD
CHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
GIVITHGTDTLEETAFFLNLVIRSDKPVVVVGSMRPGTALSADGALNLYDAVAVAASSDA
EEEEECCCCHHHHHHHHHHHEEECCCCEEEEECCCCCCEECCCCCCCHHHHHHEEECCCC
RGKGVLVTMNDEIHSGRDVSKAVNIRPDAFRSPWGPLGMVVEGKPYWFRLPAKRHTLNSE
CCCEEEEEECCCCCCCCCHHHHCCCCCHHHCCCCCCCCEEECCCCEEEECCCHHCCCCCC
FDIARIQSLAPVEIAYGYGNVSDTAYKAFAAAGAKAIIHAGTGNGSVSNRVVPGLQALRA
CCHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHH
QGVHIVRSSRVDGGGFVLRNAEQPDDKYDWVAAHDLNPQKARILAAVALTTTSDSRELQR
CCEEEEEECCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHEEEEEEEECCCCHHHHHH
IFWEY
HHHCC
>Mature Secondary Structure 
PLSSQLRHLLLPALAAAALLLPPAAHAREAAAPARLAKVVILATGGTIAGAGADAASSA
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHEEEEEEEEECCEEECCCCCCCCCC
TYQAAKVPVEKLVAGIPELKNLAEVRGEQVFQIASESFTNEHLLQLGRRVAELSASADVD
CHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
GIVITHGTDTLEETAFFLNLVIRSDKPVVVVGSMRPGTALSADGALNLYDAVAVAASSDA
EEEEECCCCHHHHHHHHHHHEEECCCCEEEEECCCCCCEECCCCCCCHHHHHHEEECCCC
RGKGVLVTMNDEIHSGRDVSKAVNIRPDAFRSPWGPLGMVVEGKPYWFRLPAKRHTLNSE
CCCEEEEEECCCCCCCCCHHHHCCCCCHHHCCCCCCCCEEECCCCEEEECCCHHCCCCCC
FDIARIQSLAPVEIAYGYGNVSDTAYKAFAAAGAKAIIHAGTGNGSVSNRVVPGLQALRA
CCHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHH
QGVHIVRSSRVDGGGFVLRNAEQPDDKYDWVAAHDLNPQKARILAAVALTTTSDSRELQR
CCEEEEEECCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHEEEEEEEECCCCHHHHHH
IFWEY
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA