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The map label for this gene is cysI [H]

Identifier: 119896723

GI number: 119896723

Start: 456824

End: 458506

Strand: Reverse

Name: cysI [H]

Synonym: azo0432

Alternate gene names: 119896723

Gene position: 458506-456824 (Counterclockwise)

Preceding gene: 119896724

Following gene: 119896722

Centisome position: 10.48

GC content: 65.95

Gene sequence:

>1683_bases
ATGTACCGATACGACGATTACGACCAGCGCCTGGTGGATGAACGGGTGGCGCAGTTCCGCGACCAGACCCGCCGCTACCT
CGCCGGCGAGCTGTCCGAGGACGAGTTCCGCCCGCTGCGCCTGCAGAACGGCCTTTACGTGCAGCGCCACGCGCCGATGC
TGCGCATCGCGGTGCCCTACGGCCTGTTCTCCAGCGCCCAGCTGCGCACGCTGGCCGACATCGCCCGCCGCTACGACCGC
GGCTACGGCCACTTCACCACCCGCCAGAACATCCAGTTCAACTGGCCGGAGCTGGCCAAGGTGCCGGAAATCCTCGCCCA
GCTCGCCACCGTGCAGATGCACGCGATCCAGACCTCGGGCAACTGCATCCGCAACATCACGTCGGACGCCTTCGCCGGCG
TGGCCGCCGACGAGCTGATCGACCCGCGCCCCTGGTGCGAGATCCTGCGCCAGTGGAGCAGCTTCCACCCGGAATTCGCC
CACCTGCCGCGCAAGTTCAAGTTCGCGGTCAATGGCGCCAAGGAAGACCGCGCGGTGATCCAGGCCCACGACATCGGCCT
GGACGTGGTGAAGAACGACGCCGGTGAGATCGGCTTCCGCGTGCTGGTCGGCGGCGGCCTCGGCCGCACTCCGATCATCG
GCGAGGAAATCGCCGCCTTCGTGCCGTGGCAGCACATCGTCACCTACTGTGAATCCATCCTGCGGGTGTACAACCTGCAC
GGCCGCCGCGACAACAAGTACAAGGCGCGCATCAAGATCCTGGTGAAGGCGCTCGGCATCGAGGAATTCCGCCGCCAGGT
CGAGGCCGAATGGGCTTTCCACAAGAACGGTCCGTCGACGCTGACCGAAGCCGAAGTCGCGCGCGTCGCCGCCCACTTCG
TCGATCCGGCCTACGAAAGCCTGCCGGCCACCGACGCCGGCTACGCCAGCCTGCTCGATGAGAACAAGCCCTTCGCCGCC
TGGGTGAAGCGCAATGTGCGCGCCCACAAGGTGCCGGGCTACGCCAGCGTCACCATTTCGCTGAAGAAGACCGGCGTGCC
CCCGGGCGACATCACCGAATCGCAGATGGAGCTGGTCGCCGACCTCGCCGACGCCTACAGCTACGGCGAGGTGCGCGCCA
CCCACCAGCAGAACCTGGTGCTGGCCGACGTGCGCCAGTCCGACCTGTTCAAGGTGTGGCAGTCGCTGCGCGACGCCGGC
CTGGCGACGCCCAACATCGGCCTGCTGACCGACATCATCTCGTGCCCGGGCGGCGACTACTGCGCGCTCGCCAACGCCAA
GTCGATCCCGATCGCCAATGCGATCCAGGAGCGTTTCGACAACCTCGACTACCTGTACGACATCGGCGAGATCGAACTCA
ACATCTCCGGCTGCATGAACGCCTGCGGCCACCACCACGTCGGCCACATCGGCGTGCTCGGCGTCGATAAGAACGGCGAG
GAGTGGTACCAGGTGACCATCGGCGGGGACCAGGGCAACAAGGCCGCGATCGGCAAGGTGATCGGCCCGTCCTTTGCCGC
TGCCGAGATGCCGGACGTCGTCACCCACCTGATCGAAACCTATATCGCCAACCGCCATGAGGACGAACGCTTCGTCGATA
CCGTGCGCCGCATCGGCATCGAACCGTTCAAGGCCCATGTCTATGGCAATCGCCAGACCGAAAAGAAGGTGGCCCATGCC
TAA

Upstream 100 bases:

>100_bases
CTCGCCACCATGCTGGTGCTGGTGGGCGCCAAGCTGATCGGCCACTGAACATCCGCGGGCGGCATTGCCGTCCGCACCCG
AACTCAACGAGAGAGACGCG

Downstream 100 bases:

>100_bases
AGTGATCCGCAACCGCCGCGTCGAGGACGACGCCCGCCAGATCGTCCGCCTTGCCGAAGGCGAAACCGCCGAAACCGTGG
CGATTCCCGCCGGCCCGGTG

Product: putative sulfite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 560

Protein sequence:

>560_residues
MYRYDDYDQRLVDERVAQFRDQTRRYLAGELSEDEFRPLRLQNGLYVQRHAPMLRIAVPYGLFSSAQLRTLADIARRYDR
GYGHFTTRQNIQFNWPELAKVPEILAQLATVQMHAIQTSGNCIRNITSDAFAGVAADELIDPRPWCEILRQWSSFHPEFA
HLPRKFKFAVNGAKEDRAVIQAHDIGLDVVKNDAGEIGFRVLVGGGLGRTPIIGEEIAAFVPWQHIVTYCESILRVYNLH
GRRDNKYKARIKILVKALGIEEFRRQVEAEWAFHKNGPSTLTEAEVARVAAHFVDPAYESLPATDAGYASLLDENKPFAA
WVKRNVRAHKVPGYASVTISLKKTGVPPGDITESQMELVADLADAYSYGEVRATHQQNLVLADVRQSDLFKVWQSLRDAG
LATPNIGLLTDIISCPGGDYCALANAKSIPIANAIQERFDNLDYLYDIGEIELNISGCMNACGHHHVGHIGVLGVDKNGE
EWYQVTIGGDQGNKAAIGKVIGPSFAAAEMPDVVTHLIETYIANRHEDERFVDTVRRIGIEPFKAHVYGNRQTEKKVAHA

Sequences:

>Translated_560_residues
MYRYDDYDQRLVDERVAQFRDQTRRYLAGELSEDEFRPLRLQNGLYVQRHAPMLRIAVPYGLFSSAQLRTLADIARRYDR
GYGHFTTRQNIQFNWPELAKVPEILAQLATVQMHAIQTSGNCIRNITSDAFAGVAADELIDPRPWCEILRQWSSFHPEFA
HLPRKFKFAVNGAKEDRAVIQAHDIGLDVVKNDAGEIGFRVLVGGGLGRTPIIGEEIAAFVPWQHIVTYCESILRVYNLH
GRRDNKYKARIKILVKALGIEEFRRQVEAEWAFHKNGPSTLTEAEVARVAAHFVDPAYESLPATDAGYASLLDENKPFAA
WVKRNVRAHKVPGYASVTISLKKTGVPPGDITESQMELVADLADAYSYGEVRATHQQNLVLADVRQSDLFKVWQSLRDAG
LATPNIGLLTDIISCPGGDYCALANAKSIPIANAIQERFDNLDYLYDIGEIELNISGCMNACGHHHVGHIGVLGVDKNGE
EWYQVTIGGDQGNKAAIGKVIGPSFAAAEMPDVVTHLIETYIANRHEDERFVDTVRRIGIEPFKAHVYGNRQTEKKVAHA
>Mature_560_residues
MYRYDDYDQRLVDERVAQFRDQTRRYLAGELSEDEFRPLRLQNGLYVQRHAPMLRIAVPYGLFSSAQLRTLADIARRYDR
GYGHFTTRQNIQFNWPELAKVPEILAQLATVQMHAIQTSGNCIRNITSDAFAGVAADELIDPRPWCEILRQWSSFHPEFA
HLPRKFKFAVNGAKEDRAVIQAHDIGLDVVKNDAGEIGFRVLVGGGLGRTPIIGEEIAAFVPWQHIVTYCESILRVYNLH
GRRDNKYKARIKILVKALGIEEFRRQVEAEWAFHKNGPSTLTEAEVARVAAHFVDPAYESLPATDAGYASLLDENKPFAA
WVKRNVRAHKVPGYASVTISLKKTGVPPGDITESQMELVADLADAYSYGEVRATHQQNLVLADVRQSDLFKVWQSLRDAG
LATPNIGLLTDIISCPGGDYCALANAKSIPIANAIQERFDNLDYLYDIGEIELNISGCMNACGHHHVGHIGVLGVDKNGE
EWYQVTIGGDQGNKAAIGKVIGPSFAAAEMPDVVTHLIETYIANRHEDERFVDTVRRIGIEPFKAHVYGNRQTEKKVAHA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=565, Percent_Identity=22.6548672566372, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322597, Length=395, Percent_Identity=25.0632911392405, Blast_Score=91, Evalue=7e-19,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62672; Mature: 62672

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRYDDYDQRLVDERVAQFRDQTRRYLAGELSEDEFRPLRLQNGLYVQRHAPMLRIAVPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCEEEEEECC
GLFSSAQLRTLADIARRYDRGYGHFTTRQNIQFNWPELAKVPEILAQLATVQMHAIQTSG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHEECCC
NCIRNITSDAFAGVAADELIDPRPWCEILRQWSSFHPEFAHLPRKFKFAVNGAKEDRAVI
HHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCEE
QAHDIGLDVVKNDAGEIGFRVLVGGGLGRTPIIGEEIAAFVPWQHIVTYCESILRVYNLH
EEHHCCCEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCC
GRRDNKYKARIKILVKALGIEEFRRQVEAEWAFHKNGPSTLTEAEVARVAAHFVDPAYES
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCHHHHH
LPATDAGYASLLDENKPFAAWVKRNVRAHKVPGYASVTISLKKTGVPPGDITESQMELVA
CCCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHH
DLADAYSYGEVRATHQQNLVLADVRQSDLFKVWQSLRDAGLATPNIGLLTDIISCPGGDY
HHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
CALANAKSIPIANAIQERFDNLDYLYDIGEIELNISGCMNACGHHHVGHIGVLGVDKNGE
EEECCCCCCCHHHHHHHHHCCCCEEEECCEEEEEHHHHHHHCCCCCCCEEEEEEECCCCC
EWYQVTIGGDQGNKAAIGKVIGPSFAAAEMPDVVTHLIETYIANRHEDERFVDTVRRIGI
EEEEEEEECCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EPFKAHVYGNRQTEKKVAHA
CCEEEEEECCCHHHHHHCCC
>Mature Secondary Structure
MYRYDDYDQRLVDERVAQFRDQTRRYLAGELSEDEFRPLRLQNGLYVQRHAPMLRIAVPY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCEEEEECCCEEEEEECC
GLFSSAQLRTLADIARRYDRGYGHFTTRQNIQFNWPELAKVPEILAQLATVQMHAIQTSG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHEECCC
NCIRNITSDAFAGVAADELIDPRPWCEILRQWSSFHPEFAHLPRKFKFAVNGAKEDRAVI
HHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCEE
QAHDIGLDVVKNDAGEIGFRVLVGGGLGRTPIIGEEIAAFVPWQHIVTYCESILRVYNLH
EEHHCCCEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCC
GRRDNKYKARIKILVKALGIEEFRRQVEAEWAFHKNGPSTLTEAEVARVAAHFVDPAYES
CCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHCHHHHH
LPATDAGYASLLDENKPFAAWVKRNVRAHKVPGYASVTISLKKTGVPPGDITESQMELVA
CCCCCCCHHHHHCCCCCHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHH
DLADAYSYGEVRATHQQNLVLADVRQSDLFKVWQSLRDAGLATPNIGLLTDIISCPGGDY
HHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCE
CALANAKSIPIANAIQERFDNLDYLYDIGEIELNISGCMNACGHHHVGHIGVLGVDKNGE
EEECCCCCCCHHHHHHHHHCCCCEEEECCEEEEEHHHHHHHCCCCCCCEEEEEEECCCCC
EWYQVTIGGDQGNKAAIGKVIGPSFAAAEMPDVVTHLIETYIANRHEDERFVDTVRRIGI
EEEEEEEECCCCCCHHHHHHHCCCHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCC
EPFKAHVYGNRQTEKKVAHA
CCEEEEEECCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA