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The map label for this gene is exsB

Identifier: 119896704

GI number: 119896704

Start: 439455

End: 440141

Strand: Reverse

Name: exsB

Synonym: azo0413

Alternate gene names: 119896704

Gene position: 440141-439455 (Counterclockwise)

Preceding gene: 119896705

Following gene: 119896703

Centisome position: 10.06

GC content: 71.32

Gene sequence:

>687_bases
ATGACCGATCTCCCGCGCGCCGTCGTCCTGCTGTCGGGCGGACTCGACTCGGCGACCTGCCTCGCCATCGCCCGCGACAT
GGGGCTGGAAACCTATGCCCTGTCGGTCGCCTACGGCCAGCGCCACGCCGCGGAACTCACGGCCTCGCGGCGCGTCGCCC
ACGCGCTCGGCGCCCGCGAACATCGCGTTGCCAGCGTCAGCCTCGGCGAGTTCGGCGGCTCGGCGCTGACCGATCCGGCG
ATCGCCGTGCCCGAAGATGCCGCGCCCGGCGGAATTCCGGTCACCTATGTACCGGCGCGCAACACCGTGATGCTGTCGAT
GGCGCTCGCGTGGGCCGAAGTGCTGGGCGCGCGCCATATCTTCGTCGGCGTCAACGCGGTGGATTATTCCGGCTATCCGG
ATTGCCGCCCGGCCTTCATCCAAGCCTTCGAGACCATGGCCAACCTCGCCACCAAGGCGGGCGTCGAGGGCCATCCGACC
ACGATCCACGCGCCGCTGATCGACCTGTCGAAGGCCGACATCATCCGTCGCGGGGTGGCGCTCGGGGTGGATTACGGCCT
CACGGTGTCGTGCTACCAGGCCGACGACGACGGCCGCGCCTGCGGCCGCTGCGACGCCTGCCGGCTGCGCCGCGAGGGCT
TCGCCGCGGCCGGCATCGCCGATCCCACGCGCTACCAGGCGCGCTGA

Upstream 100 bases:

>100_bases
CGCGACCGCCTGCCGGTCCGCTTTCAACTACAGTTGCACAAGATACTGTGGAACGACGCGCGCGGCCGCTGACCGTGCGC
CAGCCTGCTCCGGAACTGCC

Downstream 100 bases:

>100_bases
TCCCGCCCGGCGTCATGGAGGCGGCGCTCCCGGCCCCGGCCATCCTCGCCCTCGGCTTCATGCTCGGTGGCGGCTTCGGC
GCGCTCTCCAGCCGCACCCA

Product: ExsB protein

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTDLPRAVVLLSGGLDSATCLAIARDMGLETYALSVAYGQRHAAELTASRRVAHALGAREHRVASVSLGEFGGSALTDPA
IAVPEDAAPGGIPVTYVPARNTVMLSMALAWAEVLGARHIFVGVNAVDYSGYPDCRPAFIQAFETMANLATKAGVEGHPT
TIHAPLIDLSKADIIRRGVALGVDYGLTVSCYQADDDGRACGRCDACRLRREGFAAAGIADPTRYQAR

Sequences:

>Translated_228_residues
MTDLPRAVVLLSGGLDSATCLAIARDMGLETYALSVAYGQRHAAELTASRRVAHALGAREHRVASVSLGEFGGSALTDPA
IAVPEDAAPGGIPVTYVPARNTVMLSMALAWAEVLGARHIFVGVNAVDYSGYPDCRPAFIQAFETMANLATKAGVEGHPT
TIHAPLIDLSKADIIRRGVALGVDYGLTVSCYQADDDGRACGRCDACRLRREGFAAAGIADPTRYQAR
>Mature_227_residues
TDLPRAVVLLSGGLDSATCLAIARDMGLETYALSVAYGQRHAAELTASRRVAHALGAREHRVASVSLGEFGGSALTDPAI
AVPEDAAPGGIPVTYVPARNTVMLSMALAWAEVLGARHIFVGVNAVDYSGYPDCRPAFIQAFETMANLATKAGVEGHPTT
IHAPLIDLSKADIIRRGVALGVDYGLTVSCYQADDDGRACGRCDACRLRREGFAAAGIADPTRYQAR

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=229, Percent_Identity=37.117903930131, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_AZOSB (A1K2H5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931917.1
- ProteinModelPortal:   A1K2H5
- SMR:   A1K2H5
- STRING:   A1K2H5
- GeneID:   4606391
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0413
- eggNOG:   COG0603
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- PhylomeDB:   A1K2H5
- ProtClustDB:   CLSK766551
- BioCyc:   ASP62928:AZO0413-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 23928; Mature: 23797

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLPRAVVLLSGGLDSATCLAIARDMGLETYALSVAYGQRHAAELTASRRVAHALGARE
CCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHCCCH
HRVASVSLGEFGGSALTDPAIAVPEDAAPGGIPVTYVPARNTVMLSMALAWAEVLGARHI
HEEEEEECCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHCCCEE
FVGVNAVDYSGYPDCRPAFIQAFETMANLATKAGVEGHPTTIHAPLIDLSKADIIRRGVA
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHH
LGVDYGLTVSCYQADDDGRACGRCDACRLRREGFAAAGIADPTRYQAR
EECCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
TDLPRAVVLLSGGLDSATCLAIARDMGLETYALSVAYGQRHAAELTASRRVAHALGARE
CCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEEEEHHCCHHHHHHHHHHHHHHHHCCCH
HRVASVSLGEFGGSALTDPAIAVPEDAAPGGIPVTYVPARNTVMLSMALAWAEVLGARHI
HEEEEEECCCCCCCCCCCCCEECCCCCCCCCCEEEEECCCCEEHHHHHHHHHHHHCCCEE
FVGVNAVDYSGYPDCRPAFIQAFETMANLATKAGVEGHPTTIHAPLIDLSKADIIRRGVA
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHH
LGVDYGLTVSCYQADDDGRACGRCDACRLRREGFAAAGIADPTRYQAR
EECCCCCEEEEEECCCCCCCCCCCHHHHHHHCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA