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The map label for this gene is hupB [H]

Identifier: 119896606

GI number: 119896606

Start: 340402

End: 340677

Strand: Direct

Name: hupB [H]

Synonym: azo0315

Alternate gene names: 119896606

Gene position: 340402-340677 (Clockwise)

Preceding gene: 119896605

Following gene: 119896607

Centisome position: 7.78

GC content: 61.59

Gene sequence:

>276_bases
ATGAACAAAGGTGAATTCGTCGAAGCCCTGGCCGACCGTCTGGACGTATCCCGCGCTCAAGCTGACCGCGCCCTGTCCGC
CGTGCTCGACATCATCTCCGAGCAACTCGGCAAGGGTGAAAAAGTGGCGTTTACCGGCTTCGGCTCTTTCGAGGTTTCCG
AGCGTGCAGCGCGTACCGGTCGCAACCCGCAGACCGGCGCAACCATCGACATCGCTGCGTCCAGCGTGCCGAAGTTCACG
GCCGGTGCCACGCTGAAGGCTGCAGTCAACAAGTAA

Upstream 100 bases:

>100_bases
TGCCACGACCGGATGTTACCGCCTGCCCCGAATCCCGCGACGGTGCTAGAATCCGGCGCGTAGAACCACATCACTTCCCT
AATACCAATCGAGGAAAAGC

Downstream 100 bases:

>100_bases
TACCGCCTGTTCCTCGTTTTCGGACGGCCCTCGCAACCTAAGTTGCCGGGCCGTTTGCATTTTTTCGGCCTCGCGACGGA
AAAGCGGGTATAATCCGCCG

Product: DNA-binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 91; Mature: 91

Protein sequence:

>91_residues
MNKGEFVEALADRLDVSRAQADRALSAVLDIISEQLGKGEKVAFTGFGSFEVSERAARTGRNPQTGATIDIAASSVPKFT
AGATLKAAVNK

Sequences:

>Translated_91_residues
MNKGEFVEALADRLDVSRAQADRALSAVLDIISEQLGKGEKVAFTGFGSFEVSERAARTGRNPQTGATIDIAASSVPKFT
AGATLKAAVNK
>Mature_91_residues
MNKGEFVEALADRLDVSRAQADRALSAVLDIISEQLGKGEKVAFTGFGSFEVSERAARTGRNPQTGATIDIAASSVPKFT
AGATLKAAVNK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=54.4444444444444, Blast_Score=98, Evalue=1e-22,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=48.314606741573, Blast_Score=91, Evalue=1e-20,
Organism=Escherichia coli, GI1787141, Length=91, Percent_Identity=35.1648351648352, Blast_Score=67, Evalue=3e-13,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=37.0786516853933, Blast_Score=64, Evalue=2e-12,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9498; Mature: 9498

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGEFVEALADRLDVSRAQADRALSAVLDIISEQLGKGEKVAFTGFGSFEVSERAARTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
RNPQTGATIDIAASSVPKFTAGATLKAAVNK
CCCCCCCEEEECCCCCCCCCCCCCCEECCCC
>Mature Secondary Structure
MNKGEFVEALADRLDVSRAQADRALSAVLDIISEQLGKGEKVAFTGFGSFEVSERAARTG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCC
RNPQTGATIDIAASSVPKFTAGATLKAAVNK
CCCCCCCEEEECCCCCCCCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA