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The map label for this gene is oprM1 [H]

Identifier: 119896537

GI number: 119896537

Start: 274611

End: 276014

Strand: Direct

Name: oprM1 [H]

Synonym: azo0246

Alternate gene names: 119896537

Gene position: 274611-276014 (Clockwise)

Preceding gene: 119896536

Following gene: 119896538

Centisome position: 6.28

GC content: 73.15

Gene sequence:

>1404_bases
ATGCCGCTCAAGACCCTCGCGGTCGCGCTCGCCGCGGCGTCCCTCGGTGCCTGCTCCACGCTGGCGCCGGATTACGAACG
CCCGGCGGCGCCGGTGGCGGCCGCGTGGCCGGCCGAGCCGGCGCAACCCGCGGCCGGCACGCCCTCGGCCGACGCGGTGG
CGTGGCGCAGCTTCTTCGCCGACGAGCGCCTGCGCCAGGTGGTGGATCTGGCGCTGGCCAACAACCGCGACCTGCGCGTG
TCCGCCCTCAACATCGAGCGCGCCCGCGCGCTGTACCAGATCCAGCGCGCCGAGCTGGTGCCGTGGATCGGCGTTGGCGG
CGGCCAGAGCGCGCAGCGCCTGCCCGCCGACCTGACCCAGCGCGGCGAGCCCACGATCAGCCGCCAGTACAGCGCCACCG
TCGGCTTCTCCTCCTACGAGCTGGACTTCTTCGGTCGCATCCGCAGCCTGCGTGACGCGGCGCTGGAGCAATACCTCGCC
ACCGAGGACGCCCGCCGCAGCGCCCAGATCAGCCTGGTGGCGGAGGCCGCCAACGCGTGGCTGACGCTGGCCGCCGACCG
CGAGCGGCTGGATCTTGCCCGCCGTACCTACGAAACCCAGCAGCAGTCCTACGAGCTGACCAAGAAGAGCTTCGACTTCG
GCGCGGTATCGGCGATCGACCTGCGTCAGGCGCAGACCGCGCGCGAGAGCGCCCGGGCGGACCTCGCCGCCTACACCACC
CAGGTCGCACGCGACATCAACGCGCTCAACCTGGTGGCGGGCGCCGCCGTGCCCGACGAACTGCTGCCCACCGCGCTGAC
GGACAAGGTCAGCGCGGTGGGCGACCTGCCCGCCGGGCTGCCGTCCGAGGTGCTGGTCAGCCGGCCGGACATCCTCGCGG
CCGAACGCCAGCTGCGCGCGGCCTACGCCAACATCGGCGCCGCGCGCGCCGCCTTCTTCCCCAGCATCACGCTGACCGCC
GCCGCCGGCACCGCGAGCGCGTCGCTCGAAGGCTTGTTCAAGGCCGGCTCCGGCACCTGGAGTTTCGCGCCGCAGATCAA
CCTGCCGATCTTCCAGGGCGGGCGCCTGCTCGCCAACCTGCAGGTGGCCAAGGTGGACCGCGAGATCGCGGTGGCGGACT
ACGAGAAGGCGATCCAGAGCGCCTTCCGCGAGGTTGCCGATGCGCTGGCCGAACGCGCCAATGTGGGCGAGGAACTCGAC
GCGCGGCGGGCGCTGGTGGAGGCCACCGCCGAAACCTACCGCCTGTCCGAGGCGCGCTACCGCAGCGGGGTCGACAGCTA
CCTCGGCCTGCTCGACGCGCAGCGCACGCTGTACAGCGCGGAGCAGGGCCTGATCTCGGTGCGGCTGGCGGACGCCGCCA
ACCGCGTCGCGCTCTACCGCGTGCTGGGCGGCGGCGCGCAGTAA

Upstream 100 bases:

>100_bases
CGTTGTTCTACGTCGCGATCAAGCGCATCTTCAAGGACAAGCCGGCCGCCGATGCGGCGCCGCTCGCCGTCCAGGAGGCA
CGATGACCATGTCTTACCCC

Downstream 100 bases:

>100_bases
GGCCGCCGGCACAGGAACAGGGGCGTCGCGCGCAAGCCGGCGCCCCTGTTTGTTTTGGGCGGCGCCGTTCGCGCCGCTGC
TAGAATGCCGCGCATGCCGG

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MPLKTLAVALAAASLGACSTLAPDYERPAAPVAAAWPAEPAQPAAGTPSADAVAWRSFFADERLRQVVDLALANNRDLRV
SALNIERARALYQIQRAELVPWIGVGGGQSAQRLPADLTQRGEPTISRQYSATVGFSSYELDFFGRIRSLRDAALEQYLA
TEDARRSAQISLVAEAANAWLTLAADRERLDLARRTYETQQQSYELTKKSFDFGAVSAIDLRQAQTARESARADLAAYTT
QVARDINALNLVAGAAVPDELLPTALTDKVSAVGDLPAGLPSEVLVSRPDILAAERQLRAAYANIGAARAAFFPSITLTA
AAGTASASLEGLFKAGSGTWSFAPQINLPIFQGGRLLANLQVAKVDREIAVADYEKAIQSAFREVADALAERANVGEELD
ARRALVEATAETYRLSEARYRSGVDSYLGLLDAQRTLYSAEQGLISVRLADAANRVALYRVLGGGAQ

Sequences:

>Translated_467_residues
MPLKTLAVALAAASLGACSTLAPDYERPAAPVAAAWPAEPAQPAAGTPSADAVAWRSFFADERLRQVVDLALANNRDLRV
SALNIERARALYQIQRAELVPWIGVGGGQSAQRLPADLTQRGEPTISRQYSATVGFSSYELDFFGRIRSLRDAALEQYLA
TEDARRSAQISLVAEAANAWLTLAADRERLDLARRTYETQQQSYELTKKSFDFGAVSAIDLRQAQTARESARADLAAYTT
QVARDINALNLVAGAAVPDELLPTALTDKVSAVGDLPAGLPSEVLVSRPDILAAERQLRAAYANIGAARAAFFPSITLTA
AAGTASASLEGLFKAGSGTWSFAPQINLPIFQGGRLLANLQVAKVDREIAVADYEKAIQSAFREVADALAERANVGEELD
ARRALVEATAETYRLSEARYRSGVDSYLGLLDAQRTLYSAEQGLISVRLADAANRVALYRVLGGGAQ
>Mature_466_residues
PLKTLAVALAAASLGACSTLAPDYERPAAPVAAAWPAEPAQPAAGTPSADAVAWRSFFADERLRQVVDLALANNRDLRVS
ALNIERARALYQIQRAELVPWIGVGGGQSAQRLPADLTQRGEPTISRQYSATVGFSSYELDFFGRIRSLRDAALEQYLAT
EDARRSAQISLVAEAANAWLTLAADRERLDLARRTYETQQQSYELTKKSFDFGAVSAIDLRQAQTARESARADLAAYTTQ
VARDINALNLVAGAAVPDELLPTALTDKVSAVGDLPAGLPSEVLVSRPDILAAERQLRAAYANIGAARAAFFPSITLTAA
AGTASASLEGLFKAGSGTWSFAPQINLPIFQGGRLLANLQVAKVDREIAVADYEKAIQSAFREVADALAERANVGEELDA
RRALVEATAETYRLSEARYRSGVDSYLGLLDAQRTLYSAEQGLISVRLADAANRVALYRVLGGGAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=39.1397849462366, Blast_Score=320, Evalue=1e-88,
Organism=Escherichia coli, GI2367348, Length=339, Percent_Identity=28.3185840707965, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49913; Mature: 49781

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKTLAVALAAASLGACSTLAPDYERPAAPVAAAWPAEPAQPAAGTPSADAVAWRSFFA
CCHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
DERLRQVVDLALANNRDLRVSALNIERARALYQIQRAELVPWIGVGGGQSAQRLPADLTQ
HHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEECCCCCCHHHCCHHHHH
RGEPTISRQYSATVGFSSYELDFFGRIRSLRDAALEQYLATEDARRSAQISLVAEAANAW
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
LTLAADRERLDLARRTYETQQQSYELTKKSFDFGAVSAIDLRQAQTARESARADLAAYTT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QVARDINALNLVAGAAVPDELLPTALTDKVSAVGDLPAGLPSEVLVSRPDILAAERQLRA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHH
AYANIGAARAAFFPSITLTAAAGTASASLEGLFKAGSGTWSFAPQINLPIFQGGRLLANL
HHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCEEECCCCCCCEECCCEEEEEE
QVAKVDREIAVADYEKAIQSAFREVADALAERANVGEELDARRALVEATAETYRLSEARY
EHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSGVDSYLGLLDAQRTLYSAEQGLISVRLADAANRVALYRVLGGGAQ
HCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHCCCCC
>Mature Secondary Structure 
PLKTLAVALAAASLGACSTLAPDYERPAAPVAAAWPAEPAQPAAGTPSADAVAWRSFFA
CHHHHHHHHHHHHHCCHHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHH
DERLRQVVDLALANNRDLRVSALNIERARALYQIQRAELVPWIGVGGGQSAQRLPADLTQ
HHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHCCCEEECCCCCCHHHCCHHHHH
RGEPTISRQYSATVGFSSYELDFFGRIRSLRDAALEQYLATEDARRSAQISLVAEAANAW
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCE
LTLAADRERLDLARRTYETQQQSYELTKKSFDFGAVSAIDLRQAQTARESARADLAAYTT
EEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QVARDINALNLVAGAAVPDELLPTALTDKVSAVGDLPAGLPSEVLVSRPDILAAERQLRA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHH
AYANIGAARAAFFPSITLTAAAGTASASLEGLFKAGSGTWSFAPQINLPIFQGGRLLANL
HHHHCCHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCEEECCCCCCCEECCCEEEEEE
QVAKVDREIAVADYEKAIQSAFREVADALAERANVGEELDARRALVEATAETYRLSEARY
EHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
RSGVDSYLGLLDAQRTLYSAEQGLISVRLADAANRVALYRVLGGGAQ
HCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]