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The map label for this gene is hadL

Identifier: 119896507

GI number: 119896507

Start: 237835

End: 238500

Strand: Direct

Name: hadL

Synonym: azo0216

Alternate gene names: NA

Gene position: 237835-238500 (Clockwise)

Preceding gene: 119896506

Following gene: 119896508

Centisome position: 5.43

GC content: 63.81

Gene sequence:

>666_bases
ATGACCTTCCGCCCCAAGTACATCACCTTCGACTGCTACGGCACCCTGATCCGCTTCCGCATGGGCGACACCGCGCGCGC
CATGTACGCCGACCGCCTGCCGCCGGAACAGCTGGAACGCTTCGTGCACGACTTCAATGCCTACCGCCTCGATGAAGTGA
TGGGCGACTGGAAGCCCTACGTCGACATCCTCAAGAGCGCGCTGGAGCGCACCTGCAAGCGCCACGGCGTGAAGTACCTC
GAGGAAGAAGGCCAGAAGTACTACGACCTGGTGCCGACCTGGGGCCCCAACCCGGACGTGCCGGAACCGCTCGCGGTGAT
CGCCAAGGAAATCCCGCTGGTGATCCTATCGAACGCCGCCAACGAACAGATCATGACCAACGTTGAAAAGCTCGGCGCGC
CTTTCCACGCCGTCTATACCGCCGAACAGGCCCGCGCCTACAAGCCGCGCCTGCAAGCCTTCGAATACATGCTGGACCAG
CTCGGCTGCGGCCCGGAAGACATCCTCCACGTGTCCTCCAGCCTGCGCTACGACCTGATGTCGGCCGACGACCTCGGCAT
CAAGAACAAGGTCTTCGTGAACCGCGGCCACGATCCCGCCTGCGCCGCCTACAACTACACCGAGATCAAGGACATCGGCG
GCCTGCCCGCGCTGGTCGGCCTCTGA

Upstream 100 bases:

>100_bases
ATTTCATGCCGGACCCCATGCTCTAATCGATTCACGCAACCCCTCCGCCAAAGGTCGCCACTCCCCCGGCCGGCGGCACG
AACGACAGGAAATTTCGACC

Downstream 100 bases:

>100_bases
TCCTGCTGTCTCCTTCCACCGCCACCCCGAACGAGCCGCGATGAAACTCGAGTCCTACTGGACGGACAGCGCGCCGGCAT
TTGCCGGCGCCGATACCCCC

Product: haloalkanoic acid dehalogenase

Products: (R)-2-hydroxyacid; halide

Alternate protein names: HAD Family Hydrolase; Hydrolase; Haloalkanoic Acid Dehalogenase; Dehalogenase; 2-Haloacid Dehalogenase; Haloacid Dehalogenase-Like Hydrolase; Haloacid Dehalogenase; Haloacid Dehalogenase I; 2-Haloalkanoic Acid Dehalogenase; Haloacid Dehydrogenase; 2-Haloacid Halidohydrolase Type II; HAD-Superfamily Hydrolase; Haloacid Dehalogenase Type II Truncated; 2-Haloacid Halidohydrolase IVa; 2-Haloacid Halidohydrolase IVA; Dehalogenase Protein; 2-Haloalkanoic Acid Dehalogenase Protein; HAD Superfamily Haloalkanoic Acid Dehalogenase

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTFRPKYITFDCYGTLIRFRMGDTARAMYADRLPPEQLERFVHDFNAYRLDEVMGDWKPYVDILKSALERTCKRHGVKYL
EEEGQKYYDLVPTWGPNPDVPEPLAVIAKEIPLVILSNAANEQIMTNVEKLGAPFHAVYTAEQARAYKPRLQAFEYMLDQ
LGCGPEDILHVSSSLRYDLMSADDLGIKNKVFVNRGHDPACAAYNYTEIKDIGGLPALVGL

Sequences:

>Translated_221_residues
MTFRPKYITFDCYGTLIRFRMGDTARAMYADRLPPEQLERFVHDFNAYRLDEVMGDWKPYVDILKSALERTCKRHGVKYL
EEEGQKYYDLVPTWGPNPDVPEPLAVIAKEIPLVILSNAANEQIMTNVEKLGAPFHAVYTAEQARAYKPRLQAFEYMLDQ
LGCGPEDILHVSSSLRYDLMSADDLGIKNKVFVNRGHDPACAAYNYTEIKDIGGLPALVGL
>Mature_220_residues
TFRPKYITFDCYGTLIRFRMGDTARAMYADRLPPEQLERFVHDFNAYRLDEVMGDWKPYVDILKSALERTCKRHGVKYLE
EEGQKYYDLVPTWGPNPDVPEPLAVIAKEIPLVILSNAANEQIMTNVEKLGAPFHAVYTAEQARAYKPRLQAFEYMLDQL
GCGPEDILHVSSSLRYDLMSADDLGIKNKVFVNRGHDPACAAYNYTEIKDIGGLPALVGL

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.8.1.2

Molecular weight: Translated: 25129; Mature: 24998

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFRPKYITFDCYGTLIRFRMGDTARAMYADRLPPEQLERFVHDFNAYRLDEVMGDWKPY
CCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
VDILKSALERTCKRHGVKYLEEEGQKYYDLVPTWGPNPDVPEPLAVIAKEIPLVILSNAA
HHHHHHHHHHHHHHHCHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHCCEEEECCCC
NEQIMTNVEKLGAPFHAVYTAEQARAYKPRLQAFEYMLDQLGCGPEDILHVSSSLRYDLM
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHECCC
SADDLGIKNKVFVNRGHDPACAAYNYTEIKDIGGLPALVGL
CCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCHHHHCC
>Mature Secondary Structure 
TFRPKYITFDCYGTLIRFRMGDTARAMYADRLPPEQLERFVHDFNAYRLDEVMGDWKPY
CCCCCEEEEHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
VDILKSALERTCKRHGVKYLEEEGQKYYDLVPTWGPNPDVPEPLAVIAKEIPLVILSNAA
HHHHHHHHHHHHHHHCHHHHHHCCCEEEECCCCCCCCCCCCHHHHHHHHHCCEEEECCCC
NEQIMTNVEKLGAPFHAVYTAEQARAYKPRLQAFEYMLDQLGCGPEDILHVSSSLRYDLM
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHECCC
SADDLGIKNKVFVNRGHDPACAAYNYTEIKDIGGLPALVGL
CCCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-haloacid; H2O

Specific reaction: (S)-2-haloacid + H2O = (R)-2-hydroxyacid + halide

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA