Click here to switch to the map view.

The map label for this gene is sbcD [H]

Identifier: 119896490

GI number: 119896490

Start: 218051

End: 219196

Strand: Direct

Name: sbcD [H]

Synonym: azo0199

Alternate gene names: 119896490

Gene position: 218051-219196 (Clockwise)

Preceding gene: 119896488

Following gene: 119896491

Centisome position: 4.98

GC content: 72.69

Gene sequence:

>1146_bases
ATGCGTTTTCTCCACACCGCCGACTGGCATCTCGGTCGCGTCTATCACGGCGTGTCGCTGCTCGAGGACCAGGCCCATGT
GCTGCGCGATTTCGTCCGCCTTGCCGGCGAAACCCGGCCGGACGCCATCCTGATTGCCGGCGACGTCTACGACCGCTCGG
TGCCGCCCGCCGACGCGGTGCGCCTGCTCGACGAGACGCTGACCGAACTGGTGGTCGGCCTCGCCATCCCGGTGGTGCTG
ATCGCCGGCAACCACGACGGCCCCGACCGCCTCGCCTTCGGCGCCAGCCTGCTGGGCCGCGCCGGCCTTACCGTGCGCGG
GCCGGTGGATGCCAGTGTCGCCCCGGTGAGGCTGCGTGATGCCCACGGCGAGGTCGCGATCTACCCGCTGCCCTACGCCG
AACCGGCGCTGGTGCGCAGCGCGTTCGGCGAGGAAGCGCTGGCCGATCACCATGCCGCGCTCGGCGCGCAGTTGCGGGCG
ATTCGCGCCGCGCACGCGGCCGGCACCCGCTCCGTGGTGGTGGCGCACGCCTTCGTGCTCGGCGGCAGCGAATCGGAATC
GGAACGGCCGCTGTCGGTAGGCGGCAGCGGCGCGGTCGGCGCCGAACTTTTCGCAGGCTTCGATTACGTCGCCCTCGGCC
ACCTGCACCGTCCGCAGCAGGCCGGCGGCGAGCACATCCACTATTCCGGCTCGCTGCTCAAGTACAGCTTCGCCGAGGCC
GGCCACGCCAAGTCGGTGAACCTGGTGGAGATGGACGCCGCCGGGCGCTGCACGGTGGAGCGCGTCGCGCTCGCGCCGCG
CCGCGACCTGCGCATCGTCGAAGGCACGCTGGCGGACATCATCGCCGGCGCCGCCGCCGACCCCGGCCGCGACGACTACC
TGCTCGCCCGCCTCAGCGACAGCGGCGCGCTGCTCGACGCCATGGGCAAGCTGCGCACCGCCTATCCCAACGCGCTCGCC
ATCGAACGCCCGTTGCACGCGGGCGACGGCCCCGGCCGCGCGGCGGCCGACCACCGCCGCATCCGCATCGAAGACCTCTT
CGCCAGCTTCTACGCCGAAACCGCCGGCCGCCCGCTGGAAGCCGAGGCCATCGCCACCGTGCAGCGCATCGTAGGCGCCA
TCGAGCAGGAGGCGCGCCACGCATGA

Upstream 100 bases:

>100_bases
GGCAAAGCTTTCCATCGCGGCATTCTAGCGGCGGGCCGGTGCGCGGCCGCGCCGTGCGACACTCCACCGTTTTTCCGCTT
CCGCGCCTGAGGATTTCCGC

Downstream 100 bases:

>100_bases
AGCCGCTCAAGCTCAGCCTGCAGGCCTTCGGCCCCTTCGCCGGGCGCGAGGAGATCGACTTCACGCTGCTGCCGGAAGGG
GCGCTGTTCCTGATCTCCGG

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRFLHTADWHLGRVYHGVSLLEDQAHVLRDFVRLAGETRPDAILIAGDVYDRSVPPADAVRLLDETLTELVVGLAIPVVL
IAGNHDGPDRLAFGASLLGRAGLTVRGPVDASVAPVRLRDAHGEVAIYPLPYAEPALVRSAFGEEALADHHAALGAQLRA
IRAAHAAGTRSVVVAHAFVLGGSESESERPLSVGGSGAVGAELFAGFDYVALGHLHRPQQAGGEHIHYSGSLLKYSFAEA
GHAKSVNLVEMDAAGRCTVERVALAPRRDLRIVEGTLADIIAGAAADPGRDDYLLARLSDSGALLDAMGKLRTAYPNALA
IERPLHAGDGPGRAAADHRRIRIEDLFASFYAETAGRPLEAEAIATVQRIVGAIEQEARHA

Sequences:

>Translated_381_residues
MRFLHTADWHLGRVYHGVSLLEDQAHVLRDFVRLAGETRPDAILIAGDVYDRSVPPADAVRLLDETLTELVVGLAIPVVL
IAGNHDGPDRLAFGASLLGRAGLTVRGPVDASVAPVRLRDAHGEVAIYPLPYAEPALVRSAFGEEALADHHAALGAQLRA
IRAAHAAGTRSVVVAHAFVLGGSESESERPLSVGGSGAVGAELFAGFDYVALGHLHRPQQAGGEHIHYSGSLLKYSFAEA
GHAKSVNLVEMDAAGRCTVERVALAPRRDLRIVEGTLADIIAGAAADPGRDDYLLARLSDSGALLDAMGKLRTAYPNALA
IERPLHAGDGPGRAAADHRRIRIEDLFASFYAETAGRPLEAEAIATVQRIVGAIEQEARHA
>Mature_381_residues
MRFLHTADWHLGRVYHGVSLLEDQAHVLRDFVRLAGETRPDAILIAGDVYDRSVPPADAVRLLDETLTELVVGLAIPVVL
IAGNHDGPDRLAFGASLLGRAGLTVRGPVDASVAPVRLRDAHGEVAIYPLPYAEPALVRSAFGEEALADHHAALGAQLRA
IRAAHAAGTRSVVVAHAFVLGGSESESERPLSVGGSGAVGAELFAGFDYVALGHLHRPQQAGGEHIHYSGSLLKYSFAEA
GHAKSVNLVEMDAAGRCTVERVALAPRRDLRIVEGTLADIIAGAAADPGRDDYLLARLSDSGALLDAMGKLRTAYPNALA
IERPLHAGDGPGRAAADHRRIRIEDLFASFYAETAGRPLEAEAIATVQRIVGAIEQEARHA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=292, Percent_Identity=29.1095890410959, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 40276; Mature: 40276

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTADWHLGRVYHGVSLLEDQAHVLRDFVRLAGETRPDAILIAGDVYDRSVPPADAV
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
RLLDETLTELVVGLAIPVVLIAGNHDGPDRLAFGASLLGRAGLTVRGPVDASVAPVRLRD
HHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEC
AHGEVAIYPLPYAEPALVRSAFGEEALADHHAALGAQLRAIRAAHAAGTRSVVVAHAFVL
CCCCEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHEEE
GGSESESERPLSVGGSGAVGAELFAGFDYVALGHLHRPQQAGGEHIHYSGSLLKYSFAEA
CCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCHHHCCCCEEEECCCEEEEEHHHC
GHAKSVNLVEMDAAGRCTVERVALAPRRDLRIVEGTLADIIAGAAADPGRDDYLLARLSD
CCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEEEEECC
SGALLDAMGKLRTAYPNALAIERPLHAGDGPGRAAADHRRIRIEDLFASFYAETAGRPLE
CCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCC
AEAIATVQRIVGAIEQEARHA
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRFLHTADWHLGRVYHGVSLLEDQAHVLRDFVRLAGETRPDAILIAGDVYDRSVPPADAV
CCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
RLLDETLTELVVGLAIPVVLIAGNHDGPDRLAFGASLLGRAGLTVRGPVDASVAPVRLRD
HHHHHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEC
AHGEVAIYPLPYAEPALVRSAFGEEALADHHAALGAQLRAIRAAHAAGTRSVVVAHAFVL
CCCCEEEEECCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEEEHEEE
GGSESESERPLSVGGSGAVGAELFAGFDYVALGHLHRPQQAGGEHIHYSGSLLKYSFAEA
CCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCHHHCCCCEEEECCCEEEEEHHHC
GHAKSVNLVEMDAAGRCTVERVALAPRRDLRIVEGTLADIIAGAAADPGRDDYLLARLSD
CCCCCCEEEEECCCCCCCHHHHHCCCCCCCEEHHHHHHHHHHCCCCCCCCCCEEEEEECC
SGALLDAMGKLRTAYPNALAIERPLHAGDGPGRAAADHRRIRIEDLFASFYAETAGRPLE
CCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCC
AEAIATVQRIVGAIEQEARHA
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA