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The map label for this gene is rep [H]

Identifier: 119896478

GI number: 119896478

Start: 201756

End: 203750

Strand: Direct

Name: rep [H]

Synonym: azo0186

Alternate gene names: 119896478

Gene position: 201756-203750 (Clockwise)

Preceding gene: 119896477

Following gene: 119896481

Centisome position: 4.61

GC content: 63.51

Gene sequence:

>1995_bases
ATGTCCGCCCTGCTCAATCCGCCGCAACGCGAAGCCATCCACTACCTGAATGGCCCCTGTCTGGTCCTGGCCGGAGCCGG
CAGCGGGAAGACCCGCGTCATCACCCACAAGATCGCGCATCTGATCAACGAGTGCGGGCTGAGCCCCACCAATGTCGGTG
CGATCACCTTCACCAACAAGGCGGCGAAGGAGATGCAGGAGCGCGTCGCGCATCTGATGGGCGGGCGGGCGCCCGGTGGG
CTTACGGTGTGCACCTTTCACGCGCTCGGCGTGCGCATCATCCGGCAGGAGGCGCTGCACTGCGGACTGAAGCCTCAGTT
TTCCATCCTCGACGCCTCGGATGCGGTGCAAATCGTGGCCGATGTCGCCGGCGACCAGGACAAAGGCATCGCCAAGCAGA
TGCAGTGGCAGATCTCGTCGTGGAAGAACGCCATGATCTCCCCCGCCGAAGCGGCGCAACTGGCGGACAACGAGCTGTCG
GCCGCCGCGGCCAAGGCGTTTGCCGACTACGAGCGGACGTTGCGGGCCTACCAGGCGGTGGATTTCGACGACCTCATTTC
GCTGCCCGTCCGACTGTTCGAGGAAAACGACGAGGTGCGCGAGCGGTGGCAGAACCGCCTCCGCTACCTGCTCGTGGATG
AGTACCAGGATACGAACCGTGCGCAATACCGTCTGCTGCGTCAGCTCGCCGGGGTGCGGGGCGCCTTCACTGCCGTGGGC
GACGACGATCAGGCCATCTACGCCTGGCGTGGCGCCGATGTGGAAAACCTGAAGCTGCTGCAACGCGACTATCCCAAGCT
GCGCGTCATCAAGCTTGAACAGAACTACCGGTCCTCGCGGCGGATTCTCGAGGCGGCCAACGTGCTGATCGCCAACAACG
AGAAGCTGTTCGACAAGCGCCTGTGGTCCGAACACGGTCCGGGCGAACAGATCGCGGTGACCAGTTGCCGGGACGCCGAT
CACGAGGCCGAGTGGGTGGCGATGAAGATCTCCGCCCACAAGTTCGAGAACCGTACCCGGTTCAAGGACTACGCGATTCT
CTACCGCGGAAACCATCAGGCCCGCATCATCGAGCAGCAGTTGCGTAATCAACGCATTCCCTACGTGATGTCAGGGGGCC
AGAGCTTCTTCGAGAAGCCCGAAATCAAGGACCTGATCGCCTACCTGCGGCTATTGATGAACGAGGACGACGATCTCGCG
TTCATCCGCGCGATCACGATGCCGCGCCGTGGCATCGGACCGAGCACCATCGAAGCGCTGGGCCACTACGCGGGCGGGCG
CCACATCAGCCTCTTCGCCGCCAGCTTCGAGGAAGGCGTCGCGCTCCACCTCGGCGCGCGCCAGCTTGAGGCCGTCCAGC
AGTTCACCGCCTTCATCAACCGGATACAGCATCGCGCCCGTCGTGAGCCTGCGGCGCAGGTGCTGGAAGACCTGCTGGCA
GCCATTCGCTACGAAGCCTGGCTCTTCGAGCATTTCGATTCCCGCGAGGCGGAGTCAAAGTGGAGCAATGTGCGCGACTT
CGTCGGCTGGCTGGGGCGCAAGGGTGAGGAGGATGGCAAGAATCTCATCGAACTGACCCAGACGATCGCGCTGATCTCGA
TGCTCGACAAGGAGGACGCCGACTTCGACGGCGTGCAGCTTGCGACGCTGCATGCATCCAAGGGTCTTGAGTTTCCCCAT
GTGTTCCTCGTCGGCGTGGAAGAAGGCCTGCTGCCACATCAAAGCAGCATCGACGAAGACAAGGTCGAAGAGGAACGCCG
GCTGATGTATGTCGGGATCACCCGCGCACAGCGCAGTCTGAACATCACCTGGTGCGAACGCCGCAAGGCGGGCAAGGAGT
TCCGCCCGTGCGAGATTTCCCGCTTCATCGCCGAGATGGGCGGTGACCTGAACATCGGCGACCGCAAGCGGAACGCCCCC
GTGTCGCGCGAAGAGGGGCGGGCGCGGCTTGCCAACCTGAAGGCCATGTTCGAGAGGCGCGACGAAGGGAGCTAG

Upstream 100 bases:

>100_bases
GCAAAGAAGGTCTTCGGCGCGCTCTGACGACGCGCACGGGCGCGTCCGGTAAACTGCCGCCTCCCGTCGCACCGGCGGGG
TTCCCCTTGCGCCCGTTCCC

Downstream 100 bases:

>100_bases
AGGTCCCGCCCGTGCCGCGGGTTCGGACGACACAGGCAACAAAAAAGGCGGCCCGTGGGCCGCCTTTCATCCGCATCGCT
GCCGGCTTACTGCGCAACCG

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 664; Mature: 663

Protein sequence:

>664_residues
MSALLNPPQREAIHYLNGPCLVLAGAGSGKTRVITHKIAHLINECGLSPTNVGAITFTNKAAKEMQERVAHLMGGRAPGG
LTVCTFHALGVRIIRQEALHCGLKPQFSILDASDAVQIVADVAGDQDKGIAKQMQWQISSWKNAMISPAEAAQLADNELS
AAAAKAFADYERTLRAYQAVDFDDLISLPVRLFEENDEVRERWQNRLRYLLVDEYQDTNRAQYRLLRQLAGVRGAFTAVG
DDDQAIYAWRGADVENLKLLQRDYPKLRVIKLEQNYRSSRRILEAANVLIANNEKLFDKRLWSEHGPGEQIAVTSCRDAD
HEAEWVAMKISAHKFENRTRFKDYAILYRGNHQARIIEQQLRNQRIPYVMSGGQSFFEKPEIKDLIAYLRLLMNEDDDLA
FIRAITMPRRGIGPSTIEALGHYAGGRHISLFAASFEEGVALHLGARQLEAVQQFTAFINRIQHRARREPAAQVLEDLLA
AIRYEAWLFEHFDSREAESKWSNVRDFVGWLGRKGEEDGKNLIELTQTIALISMLDKEDADFDGVQLATLHASKGLEFPH
VFLVGVEEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSLNITWCERRKAGKEFRPCEISRFIAEMGGDLNIGDRKRNAP
VSREEGRARLANLKAMFERRDEGS

Sequences:

>Translated_664_residues
MSALLNPPQREAIHYLNGPCLVLAGAGSGKTRVITHKIAHLINECGLSPTNVGAITFTNKAAKEMQERVAHLMGGRAPGG
LTVCTFHALGVRIIRQEALHCGLKPQFSILDASDAVQIVADVAGDQDKGIAKQMQWQISSWKNAMISPAEAAQLADNELS
AAAAKAFADYERTLRAYQAVDFDDLISLPVRLFEENDEVRERWQNRLRYLLVDEYQDTNRAQYRLLRQLAGVRGAFTAVG
DDDQAIYAWRGADVENLKLLQRDYPKLRVIKLEQNYRSSRRILEAANVLIANNEKLFDKRLWSEHGPGEQIAVTSCRDAD
HEAEWVAMKISAHKFENRTRFKDYAILYRGNHQARIIEQQLRNQRIPYVMSGGQSFFEKPEIKDLIAYLRLLMNEDDDLA
FIRAITMPRRGIGPSTIEALGHYAGGRHISLFAASFEEGVALHLGARQLEAVQQFTAFINRIQHRARREPAAQVLEDLLA
AIRYEAWLFEHFDSREAESKWSNVRDFVGWLGRKGEEDGKNLIELTQTIALISMLDKEDADFDGVQLATLHASKGLEFPH
VFLVGVEEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSLNITWCERRKAGKEFRPCEISRFIAEMGGDLNIGDRKRNAP
VSREEGRARLANLKAMFERRDEGS
>Mature_663_residues
SALLNPPQREAIHYLNGPCLVLAGAGSGKTRVITHKIAHLINECGLSPTNVGAITFTNKAAKEMQERVAHLMGGRAPGGL
TVCTFHALGVRIIRQEALHCGLKPQFSILDASDAVQIVADVAGDQDKGIAKQMQWQISSWKNAMISPAEAAQLADNELSA
AAAKAFADYERTLRAYQAVDFDDLISLPVRLFEENDEVRERWQNRLRYLLVDEYQDTNRAQYRLLRQLAGVRGAFTAVGD
DDQAIYAWRGADVENLKLLQRDYPKLRVIKLEQNYRSSRRILEAANVLIANNEKLFDKRLWSEHGPGEQIAVTSCRDADH
EAEWVAMKISAHKFENRTRFKDYAILYRGNHQARIIEQQLRNQRIPYVMSGGQSFFEKPEIKDLIAYLRLLMNEDDDLAF
IRAITMPRRGIGPSTIEALGHYAGGRHISLFAASFEEGVALHLGARQLEAVQQFTAFINRIQHRARREPAAQVLEDLLAA
IRYEAWLFEHFDSREAESKWSNVRDFVGWLGRKGEEDGKNLIELTQTIALISMLDKEDADFDGVQLATLHASKGLEFPHV
FLVGVEEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSLNITWCERRKAGKEFRPCEISRFIAEMGGDLNIGDRKRNAPV
SREEGRARLANLKAMFERRDEGS

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=670, Percent_Identity=51.1940298507463, Blast_Score=645, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=664, Percent_Identity=37.3493975903614, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI1787196, Length=355, Percent_Identity=28.4507042253521, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=468, Percent_Identity=30.1282051282051, Blast_Score=174, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 75116; Mature: 74985

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALLNPPQREAIHYLNGPCLVLAGAGSGKTRVITHKIAHLINECGLSPTNVGAITFTNK
CCCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCH
AAKEMQERVAHLMGGRAPGGLTVCTFHALGVRIIRQEALHCGLKPQFSILDASDAVQIVA
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
DVAGDQDKGIAKQMQWQISSWKNAMISPAEAAQLADNELSAAAAKAFADYERTLRAYQAV
HHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
DFDDLISLPVRLFEENDEVRERWQNRLRYLLVDEYQDTNRAQYRLLRQLAGVRGAFTAVG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCEEEECC
DDDQAIYAWRGADVENLKLLQRDYPKLRVIKLEQNYRSSRRILEAANVLIANNEKLFDKR
CCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCEEEECCHHHHHHH
LWSEHGPGEQIAVTSCRDADHEAEWVAMKISAHKFENRTRFKDYAILYRGNHQARIIEQQ
HHHCCCCCCCEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
LRNQRIPYVMSGGQSFFEKPEIKDLIAYLRLLMNEDDDLAFIRAITMPRRGIGPSTIEAL
HHCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHEECCCCCCCCHHHHHHH
GHYAGGRHISLFAASFEEGVALHLGARQLEAVQQFTAFINRIQHRARREPAAQVLEDLLA
HHHCCCCEEEEEEECHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AIRYEAWLFEHFDSREAESKWSNVRDFVGWLGRKGEEDGKNLIELTQTIALISMLDKEDA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DFDGVQLATLHASKGLEFPHVFLVGVEEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSL
CCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCEEEEEEEHHHHCC
NITWCERRKAGKEFRPCEISRFIAEMGGDLNIGDRKRNAPVSREEGRARLANLKAMFERR
CEEEHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
DEGS
CCCC
>Mature Secondary Structure 
SALLNPPQREAIHYLNGPCLVLAGAGSGKTRVITHKIAHLINECGLSPTNVGAITFTNK
CCCCCCCHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCH
AAKEMQERVAHLMGGRAPGGLTVCTFHALGVRIIRQEALHCGLKPQFSILDASDAVQIVA
HHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHH
DVAGDQDKGIAKQMQWQISSWKNAMISPAEAAQLADNELSAAAAKAFADYERTLRAYQAV
HHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCC
DFDDLISLPVRLFEENDEVRERWQNRLRYLLVDEYQDTNRAQYRLLRQLAGVRGAFTAVG
CHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCEEEECC
DDDQAIYAWRGADVENLKLLQRDYPKLRVIKLEQNYRSSRRILEAANVLIANNEKLFDKR
CCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCEEEECCHHHHHHH
LWSEHGPGEQIAVTSCRDADHEAEWVAMKISAHKFENRTRFKDYAILYRGNHQARIIEQQ
HHHCCCCCCCEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCCCEEEEEECCCHHHHHHHH
LRNQRIPYVMSGGQSFFEKPEIKDLIAYLRLLMNEDDDLAFIRAITMPRRGIGPSTIEAL
HHCCCCCEEECCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEHHEECCCCCCCCHHHHHHH
GHYAGGRHISLFAASFEEGVALHLGARQLEAVQQFTAFINRIQHRARREPAAQVLEDLLA
HHHCCCCEEEEEEECHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
AIRYEAWLFEHFDSREAESKWSNVRDFVGWLGRKGEEDGKNLIELTQTIALISMLDKEDA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
DFDGVQLATLHASKGLEFPHVFLVGVEEGLLPHQSSIDEDKVEEERRLMYVGITRAQRSL
CCCCEEEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCEEEEEEEHHHHCC
NITWCERRKAGKEFRPCEISRFIAEMGGDLNIGDRKRNAPVSREEGRARLANLKAMFERR
CEEEHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
DEGS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]