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The map label for this gene is phoB [H]

Identifier: 119896456

GI number: 119896456

Start: 180644

End: 181354

Strand: Direct

Name: phoB [H]

Synonym: azo0164

Alternate gene names: 119896456

Gene position: 180644-181354 (Clockwise)

Preceding gene: 119896452

Following gene: 119896457

Centisome position: 4.13

GC content: 69.62

Gene sequence:

>711_bases
ATGCCTGCCAACATTCTTCTGGTCGAGGACGAGCCGGCCATCCAGGAACTGATAGCCGCCAACCTCAGCCGCGCCGGCCA
CCACGTGGTGCGTGCGGCTGATGCGGAGGCCGCCCAGCGCCTCGTGCGCGACGCCTTGCCGGACCTCGTGCTGCTGGACT
GGATGCTGCCTGGCATGTCGGGTATCGACTTCGCGCGCCGCCTGCGCGCGGAGGAACGCACCCGCGAGATTCCGATCATC
ATGCTCACCGCCCGCGGCGAGGAGCAGGACAAGGTCGCCGGCCTCGAGACCGGCGCGGACGACTACATCACCAAGCCGTT
CAGCCCGCGCGAACTCGTGGCACGGATCAAGGCGGTGCTGCGCCGCCGGGCTCCCCAGGCGACCGAGGATGCGGTCGAAC
TCGGCGGCCTGCGCCTTGATCCCGCCACTCATCGCGTCACCGCGGGTGAGCGGGCGTTGTCGCTCGGGCCCACCGAATTC
CGCCTGCTGCACTTCCTGATGACGCACGCCGAACGGGTCCATTCGCGCGCCCAACTGCTCGACCAGGTGTGGGGCGACCA
CGTCTTCGTCGAGGAGCGCACGGTCGATGTCCACATCCGCCGCCTGCGCTGCGCGCTCGAGCCGTCCGCCCACGACGCGC
TGATCCAGACGGTCCGCGGCAGCGGCTACCGTTTCTCGGCCCAGGTAGACGTCACCTCGGCGACACGCTGA

Upstream 100 bases:

>100_bases
GCCACGCCCCGCCGACCGGACCTGTTGCAGCGCGATGACGTGTCATCAAACTTTCAAAGTACTGACATACATTCGTTCGC
GGTCAATCGGAGCGAAAAAA

Downstream 100 bases:

>100_bases
GCCGTCGTCAGCGTTCTTCGGAGTTCACACCATCCGTTCCACCACCTACATCTGGACCGGCGCACTGTCCCTGCTGCTCG
CGCTGCTGGCCGTGGCCGGA

Product: phosphate regulon transcriptional regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MPANILLVEDEPAIQELIAANLSRAGHHVVRAADAEAAQRLVRDALPDLVLLDWMLPGMSGIDFARRLRAEERTREIPII
MLTARGEEQDKVAGLETGADDYITKPFSPRELVARIKAVLRRRAPQATEDAVELGGLRLDPATHRVTAGERALSLGPTEF
RLLHFLMTHAERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTVRGSGYRFSAQVDVTSATR

Sequences:

>Translated_236_residues
MPANILLVEDEPAIQELIAANLSRAGHHVVRAADAEAAQRLVRDALPDLVLLDWMLPGMSGIDFARRLRAEERTREIPII
MLTARGEEQDKVAGLETGADDYITKPFSPRELVARIKAVLRRRAPQATEDAVELGGLRLDPATHRVTAGERALSLGPTEF
RLLHFLMTHAERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTVRGSGYRFSAQVDVTSATR
>Mature_235_residues
PANILLVEDEPAIQELIAANLSRAGHHVVRAADAEAAQRLVRDALPDLVLLDWMLPGMSGIDFARRLRAEERTREIPIIM
LTARGEEQDKVAGLETGADDYITKPFSPRELVARIKAVLRRRAPQATEDAVELGGLRLDPATHRVTAGERALSLGPTEFR
LLHFLMTHAERVHSRAQLLDQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTVRGSGYRFSAQVDVTSATR

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=62.280701754386, Blast_Score=301, Evalue=3e-83,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=42.7312775330396, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=41.0480349344978, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082012, Length=227, Percent_Identity=39.2070484581498, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=36.8181818181818, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=39.9122807017544, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790860, Length=222, Percent_Identity=38.7387387387387, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=33.0316742081448, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI2367329, Length=228, Percent_Identity=40.7894736842105, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=35.0210970464135, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1789402, Length=224, Percent_Identity=36.1607142857143, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=33.8983050847458, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=34.8214285714286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=34.468085106383, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26307; Mature: 26176

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPANILLVEDEPAIQELIAANLSRAGHHVVRAADAEAAQRLVRDALPDLVLLDWMLPGMS
CCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCC
GIDFARRLRAEERTREIPIIMLTARGEEQDKVAGLETGADDYITKPFSPRELVARIKAVL
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRRAPQATEDAVELGGLRLDPATHRVTAGERALSLGPTEFRLLHFLMTHAERVHSRAQLL
HHCCCCCHHHHHHHCCEEECCCHHEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTVRGSGYRFSAQVDVTSATR
HHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
PANILLVEDEPAIQELIAANLSRAGHHVVRAADAEAAQRLVRDALPDLVLLDWMLPGMS
CCCEEEECCCHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHCCCC
GIDFARRLRAEERTREIPIIMLTARGEEQDKVAGLETGADDYITKPFSPRELVARIKAVL
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHCCCCCCCCHHCCCCCCHHHHHHHHHHHH
RRRAPQATEDAVELGGLRLDPATHRVTAGERALSLGPTEFRLLHFLMTHAERVHSRAQLL
HHCCCCCHHHHHHHCCEEECCCHHEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DQVWGDHVFVEERTVDVHIRRLRCALEPSAHDALIQTVRGSGYRFSAQVDVTSATR
HHHCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]