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The map label for this gene is gidB

Identifier: 119896434

GI number: 119896434

Start: 159946

End: 160584

Strand: Direct

Name: gidB

Synonym: azo0142

Alternate gene names: 119896434

Gene position: 159946-160584 (Clockwise)

Preceding gene: 119896433

Following gene: 119896435

Centisome position: 3.66

GC content: 68.7

Gene sequence:

>639_bases
ATGAGTGTATTGACGCCTTACGCCGGCCGGCTGGCCGACGGCATCGCCGCGCTCGGCCTCGACATCCCCACCGCCACCCA
GGCGCGGCTGATCGCGTTCGGCGAGCTGCTGCTGAAGTGGAACAAGGTCTACAACCTCACCGCGATCCGTGCGCCGCAGG
AGGTCATCACCCACCATCTGCTCGATTCGCTCGCGGTGCTGCCGTATCTGTCCGCGGTGACGCGGCTGGCCGACATCGGC
TCCGGTGGCGGCCTGCCCGGGATTCCGCTCGCCATCGTGCGCCCGGAGCTGAGCGTGATCTCGGTCGAGACGGTGAACAA
GAAGGCGAGCTTCCAGCAGCAGGCGAAAATTGAACTTGGCCTCGGCAACTTCCAGCCGCTCAATGCGCGGGTCGAGAACC
TGAAGCCGGAGCAGCCCTGCGACGGCGTGATCTCGCGCGCCTTCTCCAGCCTGAAGGATTTCGTCGAGCTGTCCGGCCAC
CTCGTCGGCGACGGCGGCGCGCTGTACGCGATGAAGGGCGTGCGCCCGGACGACGAGGTCGCCGCGCTGCCCGCCGGCTG
GACCGTGCGTGCCACCCATCCGCTGGCGGTGCCCGGCCTCGACGCCGAACGCCACCTGCTGGTGATCGCGCGTGGCTGA

Upstream 100 bases:

>100_bases
AGGCCGGCCGCATCCAGGGCATCACCCCAGCGGCGATCTCGCTGCTGCTGGTGTGGTTGAAGCGCCGCGACCTGGCGGCG
CGCAGCGAGCGCAGGTCCGC

Downstream 100 bases:

>100_bases
GGCCGTACGCATCGCCGTCATCATGGCGGCGCAGCCTGTCGCCGGCGCCGGCGGACCGCCGCCGCAATCGACTAAACTCC
GGAAAGCCTGCCGCGCGCGG

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSVLTPYAGRLADGIAALGLDIPTATQARLIAFGELLLKWNKVYNLTAIRAPQEVITHHLLDSLAVLPYLSAVTRLADIG
SGGGLPGIPLAIVRPELSVISVETVNKKASFQQQAKIELGLGNFQPLNARVENLKPEQPCDGVISRAFSSLKDFVELSGH
LVGDGGALYAMKGVRPDDEVAALPAGWTVRATHPLAVPGLDAERHLLVIARG

Sequences:

>Translated_212_residues
MSVLTPYAGRLADGIAALGLDIPTATQARLIAFGELLLKWNKVYNLTAIRAPQEVITHHLLDSLAVLPYLSAVTRLADIG
SGGGLPGIPLAIVRPELSVISVETVNKKASFQQQAKIELGLGNFQPLNARVENLKPEQPCDGVISRAFSSLKDFVELSGH
LVGDGGALYAMKGVRPDDEVAALPAGWTVRATHPLAVPGLDAERHLLVIARG
>Mature_211_residues
SVLTPYAGRLADGIAALGLDIPTATQARLIAFGELLLKWNKVYNLTAIRAPQEVITHHLLDSLAVLPYLSAVTRLADIGS
GGGLPGIPLAIVRPELSVISVETVNKKASFQQQAKIELGLGNFQPLNARVENLKPEQPCDGVISRAFSSLKDFVELSGHL
VGDGGALYAMKGVRPDDEVAALPAGWTVRATHPLAVPGLDAERHLLVIARG

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=191, Percent_Identity=47.6439790575916, Blast_Score=187, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_AZOSB (A1K1Q5)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_931647.1
- ProteinModelPortal:   A1K1Q5
- SMR:   A1K1Q5
- STRING:   A1K1Q5
- GeneID:   4608876
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo0142
- eggNOG:   COG0357
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- PhylomeDB:   A1K1Q5
- ProtClustDB:   PRK00107
- BioCyc:   ASP62928:AZO0142-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 22464; Mature: 22333

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLTPYAGRLADGIAALGLDIPTATQARLIAFGELLLKWNKVYNLTAIRAPQEVITHHL
CCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
LDSLAVLPYLSAVTRLADIGSGGGLPGIPLAIVRPELSVISVETVNKKASFQQQAKIELG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEECCCCHHHHHHHCEEEEC
LGNFQPLNARVENLKPEQPCDGVISRAFSSLKDFVELSGHLVGDGGALYAMKGVRPDDEV
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCE
AALPAGWTVRATHPLAVPGLDAERHLLVIARG
EECCCCCEEEECCCEECCCCCCCCEEEEEECC
>Mature Secondary Structure 
SVLTPYAGRLADGIAALGLDIPTATQARLIAFGELLLKWNKVYNLTAIRAPQEVITHHL
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHH
LDSLAVLPYLSAVTRLADIGSGGGLPGIPLAIVRPELSVISVETVNKKASFQQQAKIELG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCEEEEEECCCCHHHHHHHCEEEEC
LGNFQPLNARVENLKPEQPCDGVISRAFSSLKDFVELSGHLVGDGGALYAMKGVRPDDEV
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEECCCCCCCCE
AALPAGWTVRATHPLAVPGLDAERHLLVIARG
EECCCCCEEEECCCEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA