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The map label for this gene is kinE [H]

Identifier: 119896428

GI number: 119896428

Start: 151184

End: 153406

Strand: Reverse

Name: kinE [H]

Synonym: azo0136

Alternate gene names: 119896428

Gene position: 153406-151184 (Counterclockwise)

Preceding gene: 119896429

Following gene: 119896427

Centisome position: 3.51

GC content: 68.42

Gene sequence:

>2223_bases
GTGAGCCGGCTGAAGTCGATCGGCGCGGAGCTGGGCCTGCTCTTCCTGGTCGTGGCGCTGGCCCCTATCGTCACCGCGTA
CTACGTCGCCAACGACATCTTCGGCGAGGCCATCCGCCAGGAAAAACGCGAGGCCCTGACCGCGATCGCCGACGCCGCGC
ACGGCCGCATCGAGTCCTACGTGCGCACGCTGATCTCCGACACCACCACGCTGGCGAAATCGCCCAAGGTCGGCGAACTG
CTCAGTGCGCTCGAGCATCTGCCCGGCCCCTCGCCGGCCACCGCCGAATTCCTGCGCGCCTACCGCGAGGAAAAGGGCTA
CTACGACGTGATGCTGGTCGACCACCTCGGCAACATCCGGCTGTCGCTGTCGCAGCCCGAAGTGGTCGGCCAGAGCATCC
AGGGCGAACTGCTGCGCGGCAGCGCGCTCGCGCGCACCATCGACGCCGCCAATACCTTGCTGCAGACCGAGGTCTCCAAC
TTCGCGCATTTCCCGCCCAGCAAGGGCCACGCCGCCTTCCTCGCGGCGCCGGTGTTCGCCGGCGGCGTCATCATCGGCAA
CGCGGTGCTGCAGATCGACAACCGCGAGCTGGACGGCATCATCAACCGCTTCGGCGGGCTGGGCGAAAGCGGCGAGGTGC
TGGTGGGCACCATCGAGAACGCGCAGTTGCTGATCACCGCGCCGCCCCGCCACGACGCGACCTTGCGCAACCGCCAGGTG
GACGCCGCCCGCTTCGCCCCGCTGCGCGCCGCGCTCGAAGGCAGCCAGGGCACCGGCGGCTACACCGACTATCGCGGCCA
CGACACGCTGGCGGTGTGGCGCTACCTGCCGTCGCTCAACTGGGGAATGCTGGTCAAGATCGACACTGCCGAGCTGTACG
CGCCCATCCGCCGCTTCGAGGCGATCTCGCTGATGGTGATGGTGGCCTCGGTGCTGCTGGTGCTGTTCGGCGTGGCGCTG
TCCAACCGGCTGGTGTCGCGTCCGCTGGTGCGCTTCGCGCAGACGGTGAAGGCGCTGGACGAGGAATCGCTGCCGGCGGC
GGTGAATATCGACGCCCGCCACGAAATCCGCGACCTCGTCACCGCCTTCAATACGCTGATCGGCAGCGTCCGCTCGCACC
AGCACCAGCTCGAAGCGCGGGTCAGCGAACGCACCGCCGAGCTTGCCACCGCCAACGCCGGGCTGGCGCGCGCCAACCTC
GACCTGCAGGAGAGCAACGCCGAACTGGCGGTGACGCTGGACGAGCTGCAGCGCACCCACCGCCAGCTGGTCGAATCGGA
AAAGCTCGCCACGCTCGGCCAGCTCATCGCCGGCATCGCGCACGAGATCAACACCCCGCTCGGCTCGATCGCGTCGTCGA
TCGACACCATCATCGCCGGCGGCCAGCAGCAGACGGCCTTCCTCGCGCTCTACGCCGGGCTGGGCGAAGACCACCGCGCG
CTGGTGTGCAGCCTGCTGAACGTGGCCGCGACCGGCTCCAGCCTCAGCCTGAAGGAAAAGCGCGCGCTGAAGAAGGACTA
CGAGACACGGCTGGCCGAGCACCCGGCGGTGGATGCGCGCAAGGCGGCCGAGCTGTTGTCCCATCTGCCGCCGCTGGACC
TCGAACGCTACCTGCCGCTGCTGACCCACCCCGAGGCGGGCGCGATCCTCGATCAGGTCAAGCACGCGATGAACCTGCAC
CGCGCCAGCCACAACATCAAGCTCGCGTCCGAAAAGGCGCGCAAGGTGGTGTTCGCGCTGAAGAACTTCGCCCGCTTCGG
CGACCACGGCAGCAAGACGCCCACCCGGCTGCGCGACAGCATCGACACCGTGCTGACGCTGTACCACAACCAGATCAAGC
AGGCGGTGGACCTGCGCGCCGAGTTGCCCGACCTGCCGCCCATTCCCGCCTACGCCGACGAACTGGGCCAGGTGTGGATG
AACCTGATCCACAACGCGCTGCAGGCGATGGACTACTGCGGCCGGCTCGACATCACGCTGGAACAGGACGCCGAACGCGC
GGTGGTGCGCATCAGCGACACCGGCTGCGGCATCCCGGAGGAGATCCGCGAGCGCATCTTCCAGCCCTTCTTCACCACCA
AGCCCGCCGGCGAAGGCAGCGGCCTCGGGCTCGACATCGTGCGCAAGATCCTCGACAAGCACGGCGGTTCGATCCGGGTC
GAATCCGCCGTCGGCCGCGGCAGCACCTTCGAAGTCACGCTGCCTGCCACGGAGACCGCATGA

Upstream 100 bases:

>100_bases
CGCCGCATGAATGGGACGCCTTCCTGGGCCGCCTCAACGAGCGCTGGAACGGCCACTGGGCCAATCCGGGCGCCACGCCC
TCCACCGTGGAAGCGCGCTC

Downstream 100 bases:

>100_bases
ACCGCCCCCCATCCGCCCGCGCCGGAGAAGCACGATGACCGACGGCGTGATCCTCTGCGTCGATGACGAGGAAACCATCC
TCACCGCGCTCGAGATGCAG

Product: putative hybrid sensor and regulator protein

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 740; Mature: 739

Protein sequence:

>740_residues
MSRLKSIGAELGLLFLVVALAPIVTAYYVANDIFGEAIRQEKREALTAIADAAHGRIESYVRTLISDTTTLAKSPKVGEL
LSALEHLPGPSPATAEFLRAYREEKGYYDVMLVDHLGNIRLSLSQPEVVGQSIQGELLRGSALARTIDAANTLLQTEVSN
FAHFPPSKGHAAFLAAPVFAGGVIIGNAVLQIDNRELDGIINRFGGLGESGEVLVGTIENAQLLITAPPRHDATLRNRQV
DAARFAPLRAALEGSQGTGGYTDYRGHDTLAVWRYLPSLNWGMLVKIDTAELYAPIRRFEAISLMVMVASVLLVLFGVAL
SNRLVSRPLVRFAQTVKALDEESLPAAVNIDARHEIRDLVTAFNTLIGSVRSHQHQLEARVSERTAELATANAGLARANL
DLQESNAELAVTLDELQRTHRQLVESEKLATLGQLIAGIAHEINTPLGSIASSIDTIIAGGQQQTAFLALYAGLGEDHRA
LVCSLLNVAATGSSLSLKEKRALKKDYETRLAEHPAVDARKAAELLSHLPPLDLERYLPLLTHPEAGAILDQVKHAMNLH
RASHNIKLASEKARKVVFALKNFARFGDHGSKTPTRLRDSIDTVLTLYHNQIKQAVDLRAELPDLPPIPAYADELGQVWM
NLIHNALQAMDYCGRLDITLEQDAERAVVRISDTGCGIPEEIRERIFQPFFTTKPAGEGSGLGLDIVRKILDKHGGSIRV
ESAVGRGSTFEVTLPATETA

Sequences:

>Translated_740_residues
MSRLKSIGAELGLLFLVVALAPIVTAYYVANDIFGEAIRQEKREALTAIADAAHGRIESYVRTLISDTTTLAKSPKVGEL
LSALEHLPGPSPATAEFLRAYREEKGYYDVMLVDHLGNIRLSLSQPEVVGQSIQGELLRGSALARTIDAANTLLQTEVSN
FAHFPPSKGHAAFLAAPVFAGGVIIGNAVLQIDNRELDGIINRFGGLGESGEVLVGTIENAQLLITAPPRHDATLRNRQV
DAARFAPLRAALEGSQGTGGYTDYRGHDTLAVWRYLPSLNWGMLVKIDTAELYAPIRRFEAISLMVMVASVLLVLFGVAL
SNRLVSRPLVRFAQTVKALDEESLPAAVNIDARHEIRDLVTAFNTLIGSVRSHQHQLEARVSERTAELATANAGLARANL
DLQESNAELAVTLDELQRTHRQLVESEKLATLGQLIAGIAHEINTPLGSIASSIDTIIAGGQQQTAFLALYAGLGEDHRA
LVCSLLNVAATGSSLSLKEKRALKKDYETRLAEHPAVDARKAAELLSHLPPLDLERYLPLLTHPEAGAILDQVKHAMNLH
RASHNIKLASEKARKVVFALKNFARFGDHGSKTPTRLRDSIDTVLTLYHNQIKQAVDLRAELPDLPPIPAYADELGQVWM
NLIHNALQAMDYCGRLDITLEQDAERAVVRISDTGCGIPEEIRERIFQPFFTTKPAGEGSGLGLDIVRKILDKHGGSIRV
ESAVGRGSTFEVTLPATETA
>Mature_739_residues
SRLKSIGAELGLLFLVVALAPIVTAYYVANDIFGEAIRQEKREALTAIADAAHGRIESYVRTLISDTTTLAKSPKVGELL
SALEHLPGPSPATAEFLRAYREEKGYYDVMLVDHLGNIRLSLSQPEVVGQSIQGELLRGSALARTIDAANTLLQTEVSNF
AHFPPSKGHAAFLAAPVFAGGVIIGNAVLQIDNRELDGIINRFGGLGESGEVLVGTIENAQLLITAPPRHDATLRNRQVD
AARFAPLRAALEGSQGTGGYTDYRGHDTLAVWRYLPSLNWGMLVKIDTAELYAPIRRFEAISLMVMVASVLLVLFGVALS
NRLVSRPLVRFAQTVKALDEESLPAAVNIDARHEIRDLVTAFNTLIGSVRSHQHQLEARVSERTAELATANAGLARANLD
LQESNAELAVTLDELQRTHRQLVESEKLATLGQLIAGIAHEINTPLGSIASSIDTIIAGGQQQTAFLALYAGLGEDHRAL
VCSLLNVAATGSSLSLKEKRALKKDYETRLAEHPAVDARKAAELLSHLPPLDLERYLPLLTHPEAGAILDQVKHAMNLHR
ASHNIKLASEKARKVVFALKNFARFGDHGSKTPTRLRDSIDTVLTLYHNQIKQAVDLRAELPDLPPIPAYADELGQVWMN
LIHNALQAMDYCGRLDITLEQDAERAVVRISDTGCGIPEEIRERIFQPFFTTKPAGEGSGLGLDIVRKILDKHGGSIRVE
SAVGRGSTFEVTLPATETA

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=165, Percent_Identity=38.7878787878788, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1790436, Length=115, Percent_Identity=42.6086956521739, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1790300, Length=163, Percent_Identity=33.1288343558282, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789149, Length=151, Percent_Identity=33.112582781457, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI145693157, Length=119, Percent_Identity=36.1344537815126, Blast_Score=67, Evalue=5e-12,
Organism=Escherichia coli, GI87081816, Length=149, Percent_Identity=32.2147651006711, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 80309; Mature: 80178

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKSIGAELGLLFLVVALAPIVTAYYVANDIFGEAIRQEKREALTAIADAAHGRIESY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRTLISDTTTLAKSPKVGELLSALEHLPGPSPATAEFLRAYREEKGYYDVMLVDHLGNIR
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEE
LSLSQPEVVGQSIQGELLRGSALARTIDAANTLLQTEVSNFAHFPPSKGHAAFLAAPVFA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHH
GGVIIGNAVLQIDNRELDGIINRFGGLGESGEVLVGTIENAQLLITAPPRHDATLRNRQV
CCCEECCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHCCC
DAARFAPLRAALEGSQGTGGYTDYRGHDTLAVWRYLPSLNWGMLVKIDTAELYAPIRRFE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHH
AISLMVMVASVLLVLFGVALSNRLVSRPLVRFAQTVKALDEESLPAAVNIDARHEIRDLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHH
TAFNTLIGSVRSHQHQLEARVSERTAELATANAGLARANLDLQESNAELAVTLDELQRTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEHHHHHHHH
RQLVESEKLATLGQLIAGIAHEINTPLGSIASSIDTIIAGGQQQTAFLALYAGLGEDHRA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
LVCSLLNVAATGSSLSLKEKRALKKDYETRLAEHPAVDARKAAELLSHLPPLDLERYLPL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCH
LTHPEAGAILDQVKHAMNLHRASHNIKLASEKARKVVFALKNFARFGDHGSKTPTRLRDS
HCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IDTVLTLYHNQIKQAVDLRAELPDLPPIPAYADELGQVWMNLIHNALQAMDYCGRLDITL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
EQDAERAVVRISDTGCGIPEEIRERIFQPFFTTKPAGEGSGLGLDIVRKILDKHGGSIRV
CCCCCCEEEEEECCCCCCCHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
ESAVGRGSTFEVTLPATETA
EECCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SRLKSIGAELGLLFLVVALAPIVTAYYVANDIFGEAIRQEKREALTAIADAAHGRIESY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRTLISDTTTLAKSPKVGELLSALEHLPGPSPATAEFLRAYREEKGYYDVMLVDHLGNIR
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEE
LSLSQPEVVGQSIQGELLRGSALARTIDAANTLLQTEVSNFAHFPPSKGHAAFLAAPVFA
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHH
GGVIIGNAVLQIDNRELDGIINRFGGLGESGEVLVGTIENAQLLITAPPRHDATLRNRQV
CCCEECCEEEEECCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEECCCCCCCHHHHCCC
DAARFAPLRAALEGSQGTGGYTDYRGHDTLAVWRYLPSLNWGMLVKIDTAELYAPIRRFE
CHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHH
AISLMVMVASVLLVLFGVALSNRLVSRPLVRFAQTVKALDEESLPAAVNIDARHEIRDLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHH
TAFNTLIGSVRSHQHQLEARVSERTAELATANAGLARANLDLQESNAELAVTLDELQRTH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCEEEEHHHHHHHH
RQLVESEKLATLGQLIAGIAHEINTPLGSIASSIDTIIAGGQQQTAFLALYAGLGEDHRA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHH
LVCSLLNVAATGSSLSLKEKRALKKDYETRLAEHPAVDARKAAELLSHLPPLDLERYLPL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHHHCCH
LTHPEAGAILDQVKHAMNLHRASHNIKLASEKARKVVFALKNFARFGDHGSKTPTRLRDS
HCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
IDTVLTLYHNQIKQAVDLRAELPDLPPIPAYADELGQVWMNLIHNALQAMDYCGRLDITL
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEE
EQDAERAVVRISDTGCGIPEEIRERIFQPFFTTKPAGEGSGLGLDIVRKILDKHGGSIRV
CCCCCCEEEEEECCCCCCCHHHHHHHHCCHHCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
ESAVGRGSTFEVTLPATETA
EECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]