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The map label for this gene is prfC [H]

Identifier: 119896372

GI number: 119896372

Start: 91812

End: 93434

Strand: Direct

Name: prfC [H]

Synonym: azo0080

Alternate gene names: 119896372

Gene position: 91812-93434 (Clockwise)

Preceding gene: 119896368

Following gene: 119896373

Centisome position: 2.1

GC content: 65.37

Gene sequence:

>1623_bases
TTGTCCTCCACGCCTTTTCCGCCCGAGCTGCTCCGCGACGTCGACAAACGCCGCACCTTCGCGATCATTTCCCACCCCGA
CGCGGGCAAGACCACGCTGACCGAAAAGCTGCTGCTGTTCGGCGGCGCGATCCAGCTCGCCGGCACGGTGAAGGCGCGCA
AGTCCGCCCGCCACGCCACCTCCGACTGGATGGAGGTGGAAAAGCAGCGCGGCATCTCGGTGACCAGCTCGGTGATGCAG
TTCGAGTATCAGGACCACACCATCAACCTGCTCGACACCCCGGGCCACGAGGACTTCTCCGAGGACACCTACCGCGTGCT
CACCGCGGTGGATGCCGCGGTGATGGTGATCGACGCGGCCAAGGGCGTGGAAGCGCAGACCATCAAGCTGCTCGAGGTCT
GCCGCCTGCGCAACACCCCCATCATCACCTTCGTGAACAAGATGGACCGCGAGGTGCGCGAGCCCTTCGAACTGCTCGCC
GAGATCGAGGACGTGCTCAAGATCAGCTGCGCGCCGGTGACCTGGCCGATCGGCATGGGCAAGGCCTTCCGCGGCGTCTA
TCACCTGCTGCAGGACCACGTGCTCACCTTCACCCCCGGCGAGGAGCGCCGCAGCGACGCCGAAGTGCTGTCCGGCATCG
GCAACCCGGAACTCGACGCCCGCTTCCCGATGGAGATCGGCCAGGTGCGCGAAAACGTGGACCTGCTCAACGATGCCTCG
CCGCCCTTCGATCTCGACGAATTCCTCGCCGGCAAGCAGAGCCCGGTGTTCTTCGGCTCCGGTATCAACAACTTCGGCGT
TCAGGAGATCCTGCAGGCCCTGATCGACTGGGCGCCGCCGCCGCAGCAGCGCATCGCCGGCACCAAGGGCAACGACACGC
GCATGGTGCAGCCGGCCGAACCCGCCTTCACCGGCTTCGTGTTCAAGATCCAGGCCAACATGGACCCGAAGCACCGCGAC
CGCATCGCCTTCTTCCGCATCTGCTCCGGCCGCTACCAGTCGGGCATGAAGGTCAAGCACGTGCGCGCCGGGCGCGAGAT
GAAGCTCGCCAACGCGCTCACCTTCATGGCCAACGAACGGGTGCTGAAGGACGACGGCGTCGCCGGCGACATCATCGGCA
TCCACAACCACGGCCAGCTGCAGATCGGCGACACCCTGACCGAAGGCGAGATCCTCGGCTTCAAGGGCATCCCCTACTTC
TCGCCGGAAATGTTCCGCGCCGCGCGCCTGCGCGACCCCCTCAAATCCAAGCAGTTACAGAAGGGTTTGCAGGAGTTGGG
CGAAGAAGGCGCCATCCAGGTGTTCGAACAGGACGGCGGGCAGATGCTGCTCGGCGCGGTCGGCCAGCTGCAGTTCGAAG
TCGTCGCGCAGCGTCTGAAGGACGAATACAAGGTCGATGCGATCTTCGAAACCGCGGACATCCACACCGCGCGCTGGCTG
ACCTTCCCCGACGACCTCACCCGCCGCAATTTCGAGCGCGAACAGGCGCTACGGCTGGGCAAGGACGTGGATGGCAACCT
GGTGTACCTGGCCAGCAGCCGCTACAACCTGGAAGTGACGCGCGAAAAATGGCCCACGGTGGGCTTCCACGCCACCCGCG
AGCACGGCCAGGTGATCGCCTGA

Upstream 100 bases:

>100_bases
GCCCGCCGGCGCACGGAAGGCCCTGATTGCTTGGTAGAATCCGGCCACTGCGCGCTGCCGCCCGGCAGCGCCCTCCGCAA
CCCTTGATGGCCCTCTTCCC

Downstream 100 bases:

>100_bases
GCCGGCGCTCCACGACGCGATGAGCAACACCGACCACGCCGACACCCGCCGCCTCGGCCGCGTCATGGCCTGGCTGGCGG
CGCTGGCGCTGCTTGGCCTG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSSTPFPPELLRDVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKSARHATSDWMEVEKQRGISVTSSVMQ
FEYQDHTINLLDTPGHEDFSEDTYRVLTAVDAAVMVIDAAKGVEAQTIKLLEVCRLRNTPIITFVNKMDREVREPFELLA
EIEDVLKISCAPVTWPIGMGKAFRGVYHLLQDHVLTFTPGEERRSDAEVLSGIGNPELDARFPMEIGQVRENVDLLNDAS
PPFDLDEFLAGKQSPVFFGSGINNFGVQEILQALIDWAPPPQQRIAGTKGNDTRMVQPAEPAFTGFVFKIQANMDPKHRD
RIAFFRICSGRYQSGMKVKHVRAGREMKLANALTFMANERVLKDDGVAGDIIGIHNHGQLQIGDTLTEGEILGFKGIPYF
SPEMFRAARLRDPLKSKQLQKGLQELGEEGAIQVFEQDGGQMLLGAVGQLQFEVVAQRLKDEYKVDAIFETADIHTARWL
TFPDDLTRRNFEREQALRLGKDVDGNLVYLASSRYNLEVTREKWPTVGFHATREHGQVIA

Sequences:

>Translated_540_residues
MSSTPFPPELLRDVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKSARHATSDWMEVEKQRGISVTSSVMQ
FEYQDHTINLLDTPGHEDFSEDTYRVLTAVDAAVMVIDAAKGVEAQTIKLLEVCRLRNTPIITFVNKMDREVREPFELLA
EIEDVLKISCAPVTWPIGMGKAFRGVYHLLQDHVLTFTPGEERRSDAEVLSGIGNPELDARFPMEIGQVRENVDLLNDAS
PPFDLDEFLAGKQSPVFFGSGINNFGVQEILQALIDWAPPPQQRIAGTKGNDTRMVQPAEPAFTGFVFKIQANMDPKHRD
RIAFFRICSGRYQSGMKVKHVRAGREMKLANALTFMANERVLKDDGVAGDIIGIHNHGQLQIGDTLTEGEILGFKGIPYF
SPEMFRAARLRDPLKSKQLQKGLQELGEEGAIQVFEQDGGQMLLGAVGQLQFEVVAQRLKDEYKVDAIFETADIHTARWL
TFPDDLTRRNFEREQALRLGKDVDGNLVYLASSRYNLEVTREKWPTVGFHATREHGQVIA
>Mature_539_residues
SSTPFPPELLRDVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKSARHATSDWMEVEKQRGISVTSSVMQF
EYQDHTINLLDTPGHEDFSEDTYRVLTAVDAAVMVIDAAKGVEAQTIKLLEVCRLRNTPIITFVNKMDREVREPFELLAE
IEDVLKISCAPVTWPIGMGKAFRGVYHLLQDHVLTFTPGEERRSDAEVLSGIGNPELDARFPMEIGQVRENVDLLNDASP
PFDLDEFLAGKQSPVFFGSGINNFGVQEILQALIDWAPPPQQRIAGTKGNDTRMVQPAEPAFTGFVFKIQANMDPKHRDR
IAFFRICSGRYQSGMKVKHVRAGREMKLANALTFMANERVLKDDGVAGDIIGIHNHGQLQIGDTLTEGEILGFKGIPYFS
PEMFRAARLRDPLKSKQLQKGLQELGEEGAIQVFEQDGGQMLLGAVGQLQFEVVAQRLKDEYKVDAIFETADIHTARWLT
FPDDLTRRNFEREQALRLGKDVDGNLVYLASSRYNLEVTREKWPTVGFHATREHGQVIA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=504, Percent_Identity=26.984126984127, Blast_Score=159, Evalue=8e-39,
Organism=Homo sapiens, GI18390331, Length=503, Percent_Identity=26.0437375745527, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=27.8481012658228, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI25306287, Length=479, Percent_Identity=26.7223382045929, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=31.9767441860465, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI94966754, Length=167, Percent_Identity=30.5389221556886, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=33.8709677419355, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=33.8709677419355, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=33.8709677419355, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1790835, Length=538, Percent_Identity=55.5762081784387, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1789738, Length=492, Percent_Identity=28.2520325203252, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI48994988, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=487, Percent_Identity=27.5154004106776, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=485, Percent_Identity=24.7422680412371, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=35.5555555555556, Blast_Score=93, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=29.6969696969697, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=506, Percent_Identity=25.6916996047431, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=521, Percent_Identity=25.9117082533589, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=143, Percent_Identity=30.0699300699301, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=161, Percent_Identity=31.055900621118, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=161, Percent_Identity=31.055900621118, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324166, Length=153, Percent_Identity=31.3725490196078, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24582462, Length=478, Percent_Identity=26.9874476987448, Blast_Score=170, Evalue=3e-42,
Organism=Drosophila melanogaster, GI221458488, Length=540, Percent_Identity=26.1111111111111, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI78706572, Length=224, Percent_Identity=32.5892857142857, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI24585711, Length=157, Percent_Identity=31.2101910828025, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=157, Percent_Identity=31.2101910828025, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585709, Length=157, Percent_Identity=31.2101910828025, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 60449; Mature: 60318

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTPFPPELLRDVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKSARHAT
CCCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHH
SDWMEVEKQRGISVTSSVMQFEYQDHTINLLDTPGHEDFSEDTYRVLTAVDAAVMVIDAA
HHHHHHHHHCCCHHHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECC
KGVEAQTIKLLEVCRLRNTPIITFVNKMDREVREPFELLAEIEDVLKISCAPVTWPIGMG
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCCCC
KAFRGVYHLLQDHVLTFTPGEERRSDAEVLSGIGNPELDARFPMEIGQVRENVDLLNDAS
HHHHHHHHHHHCCEEEECCCHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PPFDLDEFLAGKQSPVFFGSGINNFGVQEILQALIDWAPPPQQRIAGTKGNDTRMVQPAE
CCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCEEECCCC
PAFTGFVFKIQANMDPKHRDRIAFFRICSGRYQSGMKVKHVRAGREMKLANALTFMANER
CCCCEEEEEEECCCCCCHHHHHHHEEECCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCC
VLKDDGVAGDIIGIHNHGQLQIGDTLTEGEILGFKGIPYFSPEMFRAARLRDPLKSKQLQ
EECCCCCCCCEEEECCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHH
KGLQELGEEGAIQVFEQDGGQMLLGAVGQLQFEVVAQRLKDEYKVDAIFETADIHTARWL
HHHHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEE
TFPDDLTRRNFEREQALRLGKDVDGNLVYLASSRYNLEVTREKWPTVGFHATREHGQVIA
ECCHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
SSTPFPPELLRDVDKRRTFAIISHPDAGKTTLTEKLLLFGGAIQLAGTVKARKSARHAT
CCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHHHH
SDWMEVEKQRGISVTSSVMQFEYQDHTINLLDTPGHEDFSEDTYRVLTAVDAAVMVIDAA
HHHHHHHHHCCCHHHHHHHHEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEECC
KGVEAQTIKLLEVCRLRNTPIITFVNKMDREVREPFELLAEIEDVLKISCAPVTWPIGMG
CCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEECCCCC
KAFRGVYHLLQDHVLTFTPGEERRSDAEVLSGIGNPELDARFPMEIGQVRENVDLLNDAS
HHHHHHHHHHHCCEEEECCCHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
PPFDLDEFLAGKQSPVFFGSGINNFGVQEILQALIDWAPPPQQRIAGTKGNDTRMVQPAE
CCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHCCCCCCHHHHCCCCCCCCEEECCCC
PAFTGFVFKIQANMDPKHRDRIAFFRICSGRYQSGMKVKHVRAGREMKLANALTFMANER
CCCCEEEEEEECCCCCCHHHHHHHEEECCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCC
VLKDDGVAGDIIGIHNHGQLQIGDTLTEGEILGFKGIPYFSPEMFRAARLRDPLKSKQLQ
EECCCCCCCCEEEECCCCCEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCHHHHHHHH
KGLQELGEEGAIQVFEQDGGQMLLGAVGQLQFEVVAQRLKDEYKVDAIFETADIHTARWL
HHHHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEE
TFPDDLTRRNFEREQALRLGKDVDGNLVYLASSRYNLEVTREKWPTVGFHATREHGQVIA
ECCHHHHHCCCCHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA