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The map label for this gene is rstA [H]

Identifier: 119896346

GI number: 119896346

Start: 61284

End: 62054

Strand: Reverse

Name: rstA [H]

Synonym: azo0054

Alternate gene names: 119896346

Gene position: 62054-61284 (Counterclockwise)

Preceding gene: 119896351

Following gene: 119896345

Centisome position: 1.42

GC content: 71.34

Gene sequence:

>771_bases
ATGAACCGCCCCGACCACGTGCTCATCGTCGACGACGACCCCGATATCCGCGACCTGCTCGCCGACTACCTCGGCAAGCA
GGCGCTGCAGGTATCCGCCGCGGCCGACGGCCGCCAGATGCGCGCCGCGCTGCTGGCGGGGCCGGTCGATGTGGTGGTGC
TGGACCTGATGCTGCCGGGCGAGGACGGCATGGCGCTGTTCCGCTGGCTGCGTGAGACCCCGGCCCATGCGCAGATTCCG
GTGGTGATGCTGACCGCGCGCGCCGACGACGTCGATCGCATCATCGGGCTGGAGATGGGCGCCGACGACTATCTCGGCAA
GCCCTTCGTCGCACGCGAACTGCTGGCGCGCGTGCGCGCGGTGCTGCGCCGCGCGCGCATGCTGCCGCCGGGCACGGTGC
GCACCGAAGTGGCGCGCTACCTTGCCTTTGGCGACTGGCTGCTCGACACCGTCGAACGCCACCTGGTCGCGCGCGGCGGC
ACGGTCACCTTGCTGCAGGCGGCCGAATACACCCTGCTGCGCTTCCTGCTCGACCATCCGCAGTGCGTGGTGAACCGCGA
CCAGCTGCTGGTCGGCCTGGCCGGGCGCGAGGCCGACGTGTTCGACCGCTCCATCGACCTGCGCGTCAGCCGCCTGCGCA
AGCGCCTGGGCGACGATGCGCGCGAGCCGGCCTACATCAAGACCGTGCGCAACGAGGGCTACGTGCTGTGCAAGACGGTC
GCCGCGCACGTGACGCGCCCCCCCGGCTTTGGCGACGGAGATGGCGCGTGA

Upstream 100 bases:

>100_bases
CGGCCGCGGTTTTGTATCGCGCGCTGGCCGTGCGTTGCGCCGATACACGCCGGCGAGGCGCGGATACACGGCGACACATC
CATGACGGCACAATACGGCC

Downstream 100 bases:

>100_bases
AGTCGCTTGCCGGGCGGGTGTTCCTGATCCTGGTGGTGGCCACGGTGCTGATCCAGGTGCTGAGCTTCGGCGGCGCGCTG
GCGGTGAGCGCGCGCGATTC

Product: two-component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MNRPDHVLIVDDDPDIRDLLADYLGKQALQVSAAADGRQMRAALLAGPVDVVVLDLMLPGEDGMALFRWLRETPAHAQIP
VVMLTARADDVDRIIGLEMGADDYLGKPFVARELLARVRAVLRRARMLPPGTVRTEVARYLAFGDWLLDTVERHLVARGG
TVTLLQAAEYTLLRFLLDHPQCVVNRDQLLVGLAGREADVFDRSIDLRVSRLRKRLGDDAREPAYIKTVRNEGYVLCKTV
AAHVTRPPGFGDGDGA

Sequences:

>Translated_256_residues
MNRPDHVLIVDDDPDIRDLLADYLGKQALQVSAAADGRQMRAALLAGPVDVVVLDLMLPGEDGMALFRWLRETPAHAQIP
VVMLTARADDVDRIIGLEMGADDYLGKPFVARELLARVRAVLRRARMLPPGTVRTEVARYLAFGDWLLDTVERHLVARGG
TVTLLQAAEYTLLRFLLDHPQCVVNRDQLLVGLAGREADVFDRSIDLRVSRLRKRLGDDAREPAYIKTVRNEGYVLCKTV
AAHVTRPPGFGDGDGA
>Mature_256_residues
MNRPDHVLIVDDDPDIRDLLADYLGKQALQVSAAADGRQMRAALLAGPVDVVVLDLMLPGEDGMALFRWLRETPAHAQIP
VVMLTARADDVDRIIGLEMGADDYLGKPFVARELLARVRAVLRRARMLPPGTVRTEVARYLAFGDWLLDTVERHLVARGG
TVTLLQAAEYTLLRFLLDHPQCVVNRDQLLVGLAGREADVFDRSIDLRVSRLRKRLGDDAREPAYIKTVRNEGYVLCKTV
AAHVTRPPGFGDGDGA

Specific function: The N-terminus of this protein is required for the transcriptional expression of both major outer membrane protein genes ompF and ompC; its C-terminal moiety mediates the multimerization of the ompR protein. As a multimer, it turns on the expression of th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789809, Length=237, Percent_Identity=43.0379746835443, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1790863, Length=235, Percent_Identity=36.1702127659575, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI145693140, Length=237, Percent_Identity=37.9746835443038, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788394, Length=233, Percent_Identity=36.480686695279, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787229, Length=239, Percent_Identity=35.1464435146443, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=37.1794871794872, Blast_Score=131, Evalue=3e-32,
Organism=Escherichia coli, GI1786599, Length=228, Percent_Identity=36.8421052631579, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1786784, Length=231, Percent_Identity=35.9307359307359, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1789402, Length=230, Percent_Identity=34.3478260869565, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1790860, Length=234, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI87082012, Length=229, Percent_Identity=31.8777292576419, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790552, Length=231, Percent_Identity=32.9004329004329, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=33.9130434782609, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787375, Length=231, Percent_Identity=31.6017316017316, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28323; Mature: 28323

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRPDHVLIVDDDPDIRDLLADYLGKQALQVSAAADGRQMRAALLAGPVDVVVLDLMLPG
CCCCCEEEEECCCCCHHHHHHHHHCHHHEEHHHCCCCHHHHHHHHHCCHHHEEEEEEECC
EDGMALFRWLRETPAHAQIPVVMLTARADDVDRIIGLEMGADDYLGKPFVARELLARVRA
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCCHHCCCHHHHHHHHHHHHH
VLRRARMLPPGTVRTEVARYLAFGDWLLDTVERHLVARGGTVTLLQAAEYTLLRFLLDHP
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCC
QCVVNRDQLLVGLAGREADVFDRSIDLRVSRLRKRLGDDAREPAYIKTVRNEGYVLCKTV
HHEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEHH
AAHVTRPPGFGDGDGA
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNRPDHVLIVDDDPDIRDLLADYLGKQALQVSAAADGRQMRAALLAGPVDVVVLDLMLPG
CCCCCEEEEECCCCCHHHHHHHHHCHHHEEHHHCCCCHHHHHHHHHCCHHHEEEEEEECC
EDGMALFRWLRETPAHAQIPVVMLTARADDVDRIIGLEMGADDYLGKPFVARELLARVRA
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHCCCCCCHHCCCHHHHHHHHHHHHH
VLRRARMLPPGTVRTEVARYLAFGDWLLDTVERHLVARGGTVTLLQAAEYTLLRFLLDHP
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCC
QCVVNRDQLLVGLAGREADVFDRSIDLRVSRLRKRLGDDAREPAYIKTVRNEGYVLCKTV
HHEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEHH
AAHVTRPPGFGDGDGA
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]