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The map label for this gene is rstB [H]

Identifier: 119896345

GI number: 119896345

Start: 59974

End: 61287

Strand: Reverse

Name: rstB [H]

Synonym: azo0053

Alternate gene names: 119896345

Gene position: 61287-59974 (Counterclockwise)

Preceding gene: 119896346

Following gene: 119896343

Centisome position: 1.4

GC content: 74.51

Gene sequence:

>1314_bases
GTGAAGTCGCTTGCCGGGCGGGTGTTCCTGATCCTGGTGGTGGCCACGGTGCTGATCCAGGTGCTGAGCTTCGGCGGCGC
GCTGGCGGTGAGCGCGCGCGATTCGCGGCACCAGATGTTCGAGTTCATGGGCGCCGACATCGCTTTCCTGCACCGCCTGC
TCGGGCGCATGCCGCCCGCCGAGCGCGAGACGTGGATACCGGCGCTCGACCGCGGTTTCTACCAGCCCGCGCTGGAACCG
GCCGGACGGACCCACCCCCCGGCCGATACGCCGCATCTGCGCGAATCGATGGTGCCGATGCGGCGCCTGCTCGGTCCCGC
GATCGATGTGCGCGCGGTGATGCTCGCGCCGCCCGTGCCGGGCGAAGACGGCATCCCCGCGCTGGCGGTGGCGCTGGACG
AGGCCCACACCCTGCTGGTGCGCTTTCCCACCCGCAAGGCGCCGCTCGCGCCGCCGCCGCTGGGCGTGATCGCCGCCTAT
GTGGCGGCCGTCAGCCTGGCGGTGATGCTGGCGGCGTGGGGCGCGGTGCGGCTGGCGACGCGGCCGCTGCGCCGGCTGGC
CGACGCCGCGCGGGCGCTCGGCCACAACCTCGACGCGCCGGCGCTGCCCGAGCGCGGCGCGAGCGAGCTGGTCGAAGCCA
GCCGCGCCTTCAACGCGATGCAGGCCGCGCTGCAGAAGAACCTGGCCGAACGCACCCAGATCCTCGCGGCGATTTCCCAC
GACCTCAAGACCCCGCTCACCCGCCTGCGCCTGCGCGTCGGCGCCTTGCCCGCCGATGAGCAGGCGCGCGCGCGCATCGA
GGCCGACATCGACGCGATGAGCCATCTCGTCGAGGAAGGGCTGGACTACGCCCGCAGCGCGCGCCCGAGCGAGGCGAGCA
CGCGGGTGGACCTGCAGGCGCTGGTGGAGTCGCTTGCCGAGCAGGCCGCCGACCTGGGCCAGGCGGTACGCATCGAGGGC
CGCCTGGCGGCGCCGGTGCGCTGTGCCCCGCGCGCGCTGCAGCGCGCGCTGCAGAACCTGCTCGACAACGCGCTGAAGTA
CGGCGGCGGCGCCACCACGCTGCGGCTCGCGCACGAGGGTGACGGGGTCGAGATCCGCATCGAGGACGACGGCCCCGGGC
TGCCGCCGGCGCTGCTGGAGCAGGTGTTCGAACCCTTCTTCCGCGCCGAGGATTCGCGCAGCCGCCAGACGGGTGGCGCC
GGCCTCGGCCTGGCGATTGCGCGCAACCTGCTGCGCGCGCAGGGCGGCGACATCCGGCTGGAGAACCGCGGCGGCGGCGG
GCTGGCCGCCATCGTCAGCCTGCCCCAGGGCTGA

Upstream 100 bases:

>100_bases
CGGCCTACATCAAGACCGTGCGCAACGAGGGCTACGTGCTGTGCAAGACGGTCGCCGCGCACGTGACGCGCCCCCCCGGC
TTTGGCGACGGAGATGGCGC

Downstream 100 bases:

>100_bases
TGCGGCGCCACGCCGCCGCGCTCAGCGCCGCGGCTGCAGCACGATCGGCGCCATCGGTCCGCCCTGTCCCGGCACCACGG
TATGGCGCGACCGATGGGTT

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKSLAGRVFLILVVATVLIQVLSFGGALAVSARDSRHQMFEFMGADIAFLHRLLGRMPPAERETWIPALDRGFYQPALEP
AGRTHPPADTPHLRESMVPMRRLLGPAIDVRAVMLAPPVPGEDGIPALAVALDEAHTLLVRFPTRKAPLAPPPLGVIAAY
VAAVSLAVMLAAWGAVRLATRPLRRLADAARALGHNLDAPALPERGASELVEASRAFNAMQAALQKNLAERTQILAAISH
DLKTPLTRLRLRVGALPADEQARARIEADIDAMSHLVEEGLDYARSARPSEASTRVDLQALVESLAEQAADLGQAVRIEG
RLAAPVRCAPRALQRALQNLLDNALKYGGGATTLRLAHEGDGVEIRIEDDGPGLPPALLEQVFEPFFRAEDSRSRQTGGA
GLGLAIARNLLRAQGGDIRLENRGGGGLAAIVSLPQG

Sequences:

>Translated_437_residues
MKSLAGRVFLILVVATVLIQVLSFGGALAVSARDSRHQMFEFMGADIAFLHRLLGRMPPAERETWIPALDRGFYQPALEP
AGRTHPPADTPHLRESMVPMRRLLGPAIDVRAVMLAPPVPGEDGIPALAVALDEAHTLLVRFPTRKAPLAPPPLGVIAAY
VAAVSLAVMLAAWGAVRLATRPLRRLADAARALGHNLDAPALPERGASELVEASRAFNAMQAALQKNLAERTQILAAISH
DLKTPLTRLRLRVGALPADEQARARIEADIDAMSHLVEEGLDYARSARPSEASTRVDLQALVESLAEQAADLGQAVRIEG
RLAAPVRCAPRALQRALQNLLDNALKYGGGATTLRLAHEGDGVEIRIEDDGPGLPPALLEQVFEPFFRAEDSRSRQTGGA
GLGLAIARNLLRAQGGDIRLENRGGGGLAAIVSLPQG
>Mature_437_residues
MKSLAGRVFLILVVATVLIQVLSFGGALAVSARDSRHQMFEFMGADIAFLHRLLGRMPPAERETWIPALDRGFYQPALEP
AGRTHPPADTPHLRESMVPMRRLLGPAIDVRAVMLAPPVPGEDGIPALAVALDEAHTLLVRFPTRKAPLAPPPLGVIAAY
VAAVSLAVMLAAWGAVRLATRPLRRLADAARALGHNLDAPALPERGASELVEASRAFNAMQAALQKNLAERTQILAAISH
DLKTPLTRLRLRVGALPADEQARARIEADIDAMSHLVEEGLDYARSARPSEASTRVDLQALVESLAEQAADLGQAVRIEG
RLAAPVRCAPRALQRALQNLLDNALKYGGGATTLRLAHEGDGVEIRIEDDGPGLPPALLEQVFEPFFRAEDSRSRQTGGA
GLGLAIARNLLRAQGGDIRLENRGGGGLAAIVSLPQG

Specific function: Member of the two-component regulatory system envZ/ompR involved in the regulation of osmoregulation (genes ompF and ompC). EnvZ functions as a membrane-associated protein kinase that phosphorylates ompR in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1789808, Length=282, Percent_Identity=34.0425531914894, Blast_Score=147, Evalue=9e-37,
Organism=Escherichia coli, GI1787894, Length=459, Percent_Identity=29.1938997821351, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=31.6981132075472, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1788393, Length=267, Percent_Identity=29.2134831460674, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1786912, Length=227, Percent_Identity=35.2422907488987, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790861, Length=281, Percent_Identity=32.0284697508897, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI145693157, Length=248, Percent_Identity=28.2258064516129, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082128, Length=280, Percent_Identity=29.2857142857143, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786600, Length=273, Percent_Identity=26.7399267399267, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788713, Length=257, Percent_Identity=27.6264591439689, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786783, Length=293, Percent_Identity=25.2559726962457, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789149, Length=264, Percent_Identity=28.030303030303, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=27.6785714285714, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=27.4774774774775, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081816, Length=258, Percent_Identity=29.8449612403101, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790551, Length=285, Percent_Identity=28.0701754385965, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788279, Length=320, Percent_Identity=23.125, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=25.5813953488372, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI1789403, Length=266, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=73, Percent_Identity=43.8356164383562, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 46567; Mature: 46567

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLAGRVFLILVVATVLIQVLSFGGALAVSARDSRHQMFEFMGADIAFLHRLLGRMPPA
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCC
ERETWIPALDRGFYQPALEPAGRTHPPADTPHLRESMVPMRRLLGPAIDVRAVMLAPPVP
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCEEEEECCCCC
GEDGIPALAVALDEAHTLLVRFPTRKAPLAPPPLGVIAAYVAAVSLAVMLAAWGAVRLAT
CCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPLRRLADAARALGHNLDAPALPERGASELVEASRAFNAMQAALQKNLAERTQILAAISH
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLKTPLTRLRLRVGALPADEQARARIEADIDAMSHLVEEGLDYARSARPSEASTRVDLQA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVESLAEQAADLGQAVRIEGRLAAPVRCAPRALQRALQNLLDNALKYGGGATTLRLAHEG
HHHHHHHHHHHHCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DGVEIRIEDDGPGLPPALLEQVFEPFFRAEDSRSRQTGGAGLGLAIARNLLRAQGGDIRL
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ENRGGGGLAAIVSLPQG
ECCCCCCEEEEEECCCC
>Mature Secondary Structure
MKSLAGRVFLILVVATVLIQVLSFGGALAVSARDSRHQMFEFMGADIAFLHRLLGRMPPA
CCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCC
ERETWIPALDRGFYQPALEPAGRTHPPADTPHLRESMVPMRRLLGPAIDVRAVMLAPPVP
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCEEEEECCCCC
GEDGIPALAVALDEAHTLLVRFPTRKAPLAPPPLGVIAAYVAAVSLAVMLAAWGAVRLAT
CCCCCCHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RPLRRLADAARALGHNLDAPALPERGASELVEASRAFNAMQAALQKNLAERTQILAAISH
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLKTPLTRLRLRVGALPADEQARARIEADIDAMSHLVEEGLDYARSARPSEASTRVDLQA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVESLAEQAADLGQAVRIEGRLAAPVRCAPRALQRALQNLLDNALKYGGGATTLRLAHEG
HHHHHHHHHHHHCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
DGVEIRIEDDGPGLPPALLEQVFEPFFRAEDSRSRQTGGAGLGLAIARNLLRAQGGDIRL
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEE
ENRGGGGLAAIVSLPQG
ECCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6292200; 2997120; 9278503; 8132603; 2277041; 2824492; 1323560; 9817206; 10426948 [H]