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The map label for this gene is hsdS [C]

Identifier: 119896299

GI number: 119896299

Start: 9035

End: 10384

Strand: Direct

Name: hsdS [C]

Synonym: azo0007

Alternate gene names: 119896299

Gene position: 9035-10384 (Clockwise)

Preceding gene: 119896298

Following gene: 119896300

Centisome position: 0.21

GC content: 56.44

Gene sequence:

>1350_bases
ATGAGTTTGCCGAGGTATGCGGAGTACAAGGACAGTGGGGTGGCCTTACTGGCCACGATTCCAGCCCATTGGGAACCAAG
TCCTCTAAAGCGTGTTGTTGCGCTGGTCGAGTCTGGCGTCAGCGTGAACGCAGTGGATGAACCGGCCGGCCCCGATGCGG
TTGGCGTGCTGAAAACCAGCTGCGTCTACAGTGGCAACTTTAGTCATGGCGAAAACAAGGCTGTAGTTGCCGAAGAGCTT
GACCGAGTCGCGTGCCCCGTAAGAGCTGGCACGTTGATTGTCAGCCGAATGAACACTCCGGCCCTCGTTGGGGCCGCCGG
GCTTGTGGAAGAGAACGCAGACAACCTGTTTCTTCCTGACCGACTGTGGCAGGTCCACTTCTCCGGAGCAGTTCCAAAGT
TTGCGCACTACTGGACAGCTTCCCCGTCATACCGAGCGCAAGTTCAGATGGCCTGCGCCGGCACGAGCGCCAGCATGCAG
AACCTGTCGCAAGACGAGTTTCTGCGCTTTGTCATGCCATTGCCGCCAAAAGATGAGCAAACCGCCATCGCCGCCTTCCT
CGACCGCGAGACGGCGAAAATCGATGCGCTGATTGCCAAGCAGGAAAAGCTCATCGCCCTGCTGGCCGAAAAGCGCCAGG
CCACCATCTCCCACGCCGTCACCCGCGGCCTCAACCCCGACGCGCCGATGAAGGATTCCGGCGTGGCCTGGCTGGGGGAA
GTGCCAGCGCATTGGAGCGTCAGTGCGCTAAGTTACCTAGCGTCGCTAGAGACGGGGGCTACGCCTGACCGAGGTGAGCC
AAGCTACTGGAATGGCACAATTCCTTGGTTGAAAACAGGTGAAATTAACTGGGCGCCCATATGTGAAGCGGAAGAGTTCA
TTACTGACGCAGGTCTCGAAAACTCAGCCGCAAAAATAGCGAAGCCGGGAACCTTGCTTATGGCGATGTATGGGCAAGGC
GTTACGCGTGGGCGTGTGGCCTTGCTTGAGATTGAGGCCACTTACAATCAAGCGTGTGCAGCCATTAACTTCAGGAGCCG
AATTATTCCTGAATTCGGTCGGTATTTTTTCATGGCCGCGTATGACCATGTTCGAGACGCTGGAAATGAGACAAGCCAAA
TGAATCTAAGCGCTGGGTTAATCTCAAAAATTCGTCTTCCTGTTCCTCCTTTAGATGAGCAACAAGCTGTGGTGCGTTTT
CTCGATGTTGAAACGGCAAAGCTTGATGTGCTCGGCGCAGAATCTGAGCGAGGCATTACCTTACTCAAAGAACGCCGCTC
CGCCCTCATCGCCGCCGCCGTCACCGGCCAGATCGACGTGCGTAATACGGCAGAAAGAGCCAGCACATGA

Upstream 100 bases:

>100_bases
ACCGTCGCGCGCATCCTCGCCCGTGCAGCCTGCACGCCATCGATGAGGAGCTGAAGACCGTCTCGGCCAACATCATGAAG
ATGCTGGAGGGCCTGGCGGA

Downstream 100 bases:

>100_bases
AAACCCAAGCCAAGACGATCCAGATCTTCCTGCCCGGCGGTGATCCGCAGGGCATCCGCGTGGCGGAGATCACGACGCGC
ATCGTGCAGCTGATCGAAGT

Product: Type I site-specific deoxyribonuclease

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSLPRYAEYKDSGVALLATIPAHWEPSPLKRVVALVESGVSVNAVDEPAGPDAVGVLKTSCVYSGNFSHGENKAVVAEEL
DRVACPVRAGTLIVSRMNTPALVGAAGLVEENADNLFLPDRLWQVHFSGAVPKFAHYWTASPSYRAQVQMACAGTSASMQ
NLSQDEFLRFVMPLPPKDEQTAIAAFLDRETAKIDALIAKQEKLIALLAEKRQATISHAVTRGLNPDAPMKDSGVAWLGE
VPAHWSVSALSYLASLETGATPDRGEPSYWNGTIPWLKTGEINWAPICEAEEFITDAGLENSAAKIAKPGTLLMAMYGQG
VTRGRVALLEIEATYNQACAAINFRSRIIPEFGRYFFMAAYDHVRDAGNETSQMNLSAGLISKIRLPVPPLDEQQAVVRF
LDVETAKLDVLGAESERGITLLKERRSALIAAAVTGQIDVRNTAERAST

Sequences:

>Translated_449_residues
MSLPRYAEYKDSGVALLATIPAHWEPSPLKRVVALVESGVSVNAVDEPAGPDAVGVLKTSCVYSGNFSHGENKAVVAEEL
DRVACPVRAGTLIVSRMNTPALVGAAGLVEENADNLFLPDRLWQVHFSGAVPKFAHYWTASPSYRAQVQMACAGTSASMQ
NLSQDEFLRFVMPLPPKDEQTAIAAFLDRETAKIDALIAKQEKLIALLAEKRQATISHAVTRGLNPDAPMKDSGVAWLGE
VPAHWSVSALSYLASLETGATPDRGEPSYWNGTIPWLKTGEINWAPICEAEEFITDAGLENSAAKIAKPGTLLMAMYGQG
VTRGRVALLEIEATYNQACAAINFRSRIIPEFGRYFFMAAYDHVRDAGNETSQMNLSAGLISKIRLPVPPLDEQQAVVRF
LDVETAKLDVLGAESERGITLLKERRSALIAAAVTGQIDVRNTAERAST
>Mature_448_residues
SLPRYAEYKDSGVALLATIPAHWEPSPLKRVVALVESGVSVNAVDEPAGPDAVGVLKTSCVYSGNFSHGENKAVVAEELD
RVACPVRAGTLIVSRMNTPALVGAAGLVEENADNLFLPDRLWQVHFSGAVPKFAHYWTASPSYRAQVQMACAGTSASMQN
LSQDEFLRFVMPLPPKDEQTAIAAFLDRETAKIDALIAKQEKLIALLAEKRQATISHAVTRGLNPDAPMKDSGVAWLGEV
PAHWSVSALSYLASLETGATPDRGEPSYWNGTIPWLKTGEINWAPICEAEEFITDAGLENSAAKIAKPGTLLMAMYGQGV
TRGRVALLEIEATYNQACAAINFRSRIIPEFGRYFFMAAYDHVRDAGNETSQMNLSAGLISKIRLPVPPLDEQQAVVRFL
DVETAKLDVLGAESERGITLLKERRSALIAAAVTGQIDVRNTAERAST

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 48507; Mature: 48375

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPRYAEYKDSGVALLATIPAHWEPSPLKRVVALVESGVSVNAVDEPAGPDAVGVLKTS
CCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHEEEE
CVYSGNFSHGENKAVVAEELDRVACPVRAGTLIVSRMNTPALVGAAGLVEENADNLFLPD
EEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHCCCHHHHCCCCCCEECCC
RLWQVHFSGAVPKFAHYWTASPSYRAQVQMACAGTSASMQNLSQDEFLRFVMPLPPKDEQ
HHEEEEECCCCCHHHHEECCCCCCCEEEEEEECCCCHHHHHCCHHHHHHHCCCCCCCCCH
TAIAAFLDRETAKIDALIAKQEKLIALLAEKRQATISHAVTRGLNPDAPMKDSGVAWLGE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
VPAHWSVSALSYLASLETGATPDRGEPSYWNGTIPWLKTGEINWAPICEAEEFITDAGLE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCC
NSAAKIAKPGTLLMAMYGQGVTRGRVALLEIEATYNQACAAINFRSRIIPEFGRYFFMAA
CCCHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCHHHEECHHHHHHHHHHHHHHHHH
YDHVRDAGNETSQMNLSAGLISKIRLPVPPLDEQQAVVRFLDVETAKLDVLGAESERGIT
HHHHHHCCCCCCEEEEHHHHHHHEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHH
LLKERRSALIAAAVTGQIDVRNTAERAST
HHHHHHHHEEEEEEECCEECCCCHHHCCC
>Mature Secondary Structure 
SLPRYAEYKDSGVALLATIPAHWEPSPLKRVVALVESGVSVNAVDEPAGPDAVGVLKTS
CCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHEEEE
CVYSGNFSHGENKAVVAEELDRVACPVRAGTLIVSRMNTPALVGAAGLVEENADNLFLPD
EEECCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHCCCHHHHCCCCCCEECCC
RLWQVHFSGAVPKFAHYWTASPSYRAQVQMACAGTSASMQNLSQDEFLRFVMPLPPKDEQ
HHEEEEECCCCCHHHHEECCCCCCCEEEEEEECCCCHHHHHCCHHHHHHHCCCCCCCCCH
TAIAAFLDRETAKIDALIAKQEKLIALLAEKRQATISHAVTRGLNPDAPMKDSGVAWLGE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECC
VPAHWSVSALSYLASLETGATPDRGEPSYWNGTIPWLKTGEINWAPICEAEEFITDAGLE
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCHHHHHHHCCCC
NSAAKIAKPGTLLMAMYGQGVTRGRVALLEIEATYNQACAAINFRSRIIPEFGRYFFMAA
CCCHHHCCCCEEEEEECCCCCCCCCEEEEEEECCCCCHHHEECHHHHHHHHHHHHHHHHH
YDHVRDAGNETSQMNLSAGLISKIRLPVPPLDEQQAVVRFLDVETAKLDVLGAESERGIT
HHHHHHCCCCCCEEEEHHHHHHHEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHH
LLKERRSALIAAAVTGQIDVRNTAERAST
HHHHHHHHEEEEEEECCEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]