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The map label for this gene is acrA [H]

Identifier: 119776685

GI number: 119776685

Start: 4184976

End: 4186112

Strand: Direct

Name: acrA [H]

Synonym: Sama_3553

Alternate gene names: 119776685

Gene position: 4184976-4186112 (Clockwise)

Preceding gene: 119776683

Following gene: 119776686

Centisome position: 97.19

GC content: 57.34

Gene sequence:

>1137_bases
ATGCGGCAAATGTTAAAAATTGCCTCGGCCATAGGCATGGTCTTGTGGGTCAGTGCCTGCCAGGAAGATGCTCAGCAAGC
TGCCAACCAGCAGCAAATGCCTCCCATGGAAGTCGGCACAGTGCAGGTCACTGCAGCGCCTCAAAACATCATGGTTGAGC
TGCCCGGTCGCAGCCGCGCTTTCCTCGAGGCCGAGGTTCGCCCTCAGGTTTCTGGCATCATCATGGAGCGTAACTTCGTT
GAAGGTCGTGAAGTGAAAGAAGGTCAGTCACTCTACAAAATTGACGATGCCACCTATAAGGCCACCCTCGAAAGCGCCAA
GGCCGACCTCGCCAGCGCACAGGCCTCATTGGCCAGTGCCAAAGCCCGCGCCGCCCGCTACGAAAAGTTGGTGAAAACCA
ATGCCATTTCCAAGCAGGACTTCGACGAAGCCGATGCCGCCTATAAGGAAGCACTCGCCCGGGTGAAAGTGGCCGAGGCC
CAGCTGCATACCGCCAATATCAATCTGAACTACACCGAAGTGCAGGCCCCAATTTCAGGCCGCATCGGTAAATCGTCCGT
CACGCCTGGTGCGTTGGTAACCGCCAATCAGGGACAGGTACTGGCCACTATTCAGCAGCTGGACCCCATCAATGTGGACA
TCACCCAATCCAGCGCCCAACTGCTGGCCCTCAAGGCCAAGCTGCGAGCCGGTAAGCTGCAGGCGGCCGACAACGCCGAA
GTGCAGCTGGTACTGGAAGATGGCACAACTTACGAGCACAAGGGCAAGCTGCAATTTGCCGAAGTGAGCGTGGATGAAAA
CACGGGTTCTGTGATTCTGCGGGCCGAGTTCCCCAACCCAGACAACGTGTTGCTGCCGGGTATGTATGTACGCGCCATGC
TGAACACCGGGGTAGATCCCAACGCCATTCTGGTGCCGCAAAAGGCCATTACCCGCAATACCCGTGGTGAAGCCGTGGCC
ATGGTGGTGAACGCCGAAAGCAAGGTTGAAGCCCGCACCGTGACCACTGCCGAGGTTATCAACCACCAGTGGCGTATTAC
TTCGGGGCTGAATGCAGGCGATAAATTGATTGTAGAAGGGCTGCAGAAAATACGCCCAGGTGCACCGGTCACTGCCACAG
CGGCGCAGGCCAAGTAA

Upstream 100 bases:

>100_bases
CTGATCATAAATTCAGTATTAAAAATGGGCGATTTGTCTGGGTATCGAGCGCTTTTTATCCGCACAACCCCGTGAGCCCA
GACATAAAAAGGAGATTTCA

Downstream 100 bases:

>100_bases
TTGGCAAAGGACCCCAGGAACCCGCGAAATTCAGGATTAAGCTATGGCACGTTTTTTTATTGATCGACCCATTTTTGCAT
GGGTGATCGCGATTTTGGTG

Product: multidrug resistance protein AcrA/AcrE family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MRQMLKIASAIGMVLWVSACQEDAQQAANQQQMPPMEVGTVQVTAAPQNIMVELPGRSRAFLEAEVRPQVSGIIMERNFV
EGREVKEGQSLYKIDDATYKATLESAKADLASAQASLASAKARAARYEKLVKTNAISKQDFDEADAAYKEALARVKVAEA
QLHTANINLNYTEVQAPISGRIGKSSVTPGALVTANQGQVLATIQQLDPINVDITQSSAQLLALKAKLRAGKLQAADNAE
VQLVLEDGTTYEHKGKLQFAEVSVDENTGSVILRAEFPNPDNVLLPGMYVRAMLNTGVDPNAILVPQKAITRNTRGEAVA
MVVNAESKVEARTVTTAEVINHQWRITSGLNAGDKLIVEGLQKIRPGAPVTATAAQAK

Sequences:

>Translated_378_residues
MRQMLKIASAIGMVLWVSACQEDAQQAANQQQMPPMEVGTVQVTAAPQNIMVELPGRSRAFLEAEVRPQVSGIIMERNFV
EGREVKEGQSLYKIDDATYKATLESAKADLASAQASLASAKARAARYEKLVKTNAISKQDFDEADAAYKEALARVKVAEA
QLHTANINLNYTEVQAPISGRIGKSSVTPGALVTANQGQVLATIQQLDPINVDITQSSAQLLALKAKLRAGKLQAADNAE
VQLVLEDGTTYEHKGKLQFAEVSVDENTGSVILRAEFPNPDNVLLPGMYVRAMLNTGVDPNAILVPQKAITRNTRGEAVA
MVVNAESKVEARTVTTAEVINHQWRITSGLNAGDKLIVEGLQKIRPGAPVTATAAQAK
>Mature_378_residues
MRQMLKIASAIGMVLWVSACQEDAQQAANQQQMPPMEVGTVQVTAAPQNIMVELPGRSRAFLEAEVRPQVSGIIMERNFV
EGREVKEGQSLYKIDDATYKATLESAKADLASAQASLASAKARAARYEKLVKTNAISKQDFDEADAAYKEALARVKVAEA
QLHTANINLNYTEVQAPISGRIGKSSVTPGALVTANQGQVLATIQQLDPINVDITQSSAQLLALKAKLRAGKLQAADNAE
VQLVLEDGTTYEHKGKLQFAEVSVDENTGSVILRAEFPNPDNVLLPGMYVRAMLNTGVDPNAILVPQKAITRNTRGEAVA
MVVNAESKVEARTVTTAEVINHQWRITSGLNAGDKLIVEGLQKIRPGAPVTATAAQAK

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=359, Percent_Identity=52.6462395543176, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI1789665, Length=363, Percent_Identity=50.4132231404959, Blast_Score=340, Evalue=7e-95,
Organism=Escherichia coli, GI1789929, Length=352, Percent_Identity=47.7272727272727, Blast_Score=321, Evalue=5e-89,
Organism=Escherichia coli, GI87082036, Length=315, Percent_Identity=29.8412698412698, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI87081796, Length=333, Percent_Identity=26.4264264264264, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789637, Length=127, Percent_Identity=31.496062992126, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87081951, Length=127, Percent_Identity=37.007874015748, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40564; Mature: 40564

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQMLKIASAIGMVLWVSACQEDAQQAANQQQMPPMEVGTVQVTAAPQNIMVELPGRSRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCCE
FLEAEVRPQVSGIIMERNFVEGREVKEGQSLYKIDDATYKATLESAKADLASAQASLASA
EEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KARAARYEKLVKTNAISKQDFDEADAAYKEALARVKVAEAQLHTANINLNYTEVQAPISG
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEECCCCC
RIGKSSVTPGALVTANQGQVLATIQQLDPINVDITQSSAQLLALKAKLRAGKLQAADNAE
CCCCCCCCCCEEEECCCCCEEEEEHHCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCE
VQLVLEDGTTYEHKGKLQFAEVSVDENTGSVILRAEFPNPDNVLLPGMYVRAMLNTGVDP
EEEEEECCCCEECCCCEEEEEEEECCCCCCEEEEEECCCCCCEEECHHHHHHHHHCCCCC
NAILVPQKAITRNTRGEAVAMVVNAESKVEARTVTTAEVINHQWRITSGLNAGDKLIVEG
CEEEECCHHHHCCCCCCEEEEEEECCCCCHHHEEHHHHHHCCCEEEECCCCCCHHHHHHH
LQKIRPGAPVTATAAQAK
HHHHCCCCCEECCCCCCC
>Mature Secondary Structure
MRQMLKIASAIGMVLWVSACQEDAQQAANQQQMPPMEVGTVQVTAAPQNIMVELPGRSRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEEEECCCCCCE
FLEAEVRPQVSGIIMERNFVEGREVKEGQSLYKIDDATYKATLESAKADLASAQASLASA
EEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
KARAARYEKLVKTNAISKQDFDEADAAYKEALARVKVAEAQLHTANINLNYTEVQAPISG
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEEECCCCC
RIGKSSVTPGALVTANQGQVLATIQQLDPINVDITQSSAQLLALKAKLRAGKLQAADNAE
CCCCCCCCCCEEEECCCCCEEEEEHHCCCCEEEEECCCHHHHHHHHHHCCCCEECCCCCE
VQLVLEDGTTYEHKGKLQFAEVSVDENTGSVILRAEFPNPDNVLLPGMYVRAMLNTGVDP
EEEEEECCCCEECCCCEEEEEEEECCCCCCEEEEEECCCCCCEEECHHHHHHHHHCCCCC
NAILVPQKAITRNTRGEAVAMVVNAESKVEARTVTTAEVINHQWRITSGLNAGDKLIVEG
CEEEECCHHHHCCCCCCEEEEEEECCCCCHHHEEHHHHHHCCCEEEECCCCCCHHHHHHH
LQKIRPGAPVTATAAQAK
HHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]