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The map label for this gene is rpoH [H]

Identifier: 119776597

GI number: 119776597

Start: 4095007

End: 4095864

Strand: Reverse

Name: rpoH [H]

Synonym: Sama_3465

Alternate gene names: 119776597

Gene position: 4095864-4095007 (Counterclockwise)

Preceding gene: 119776598

Following gene: 119776596

Centisome position: 95.12

GC content: 53.15

Gene sequence:

>858_bases
ATGACTGGACAAACGCAATCAATGGCACTGATGGTCCCCCAGGGAGCCAGTAGTCTTGAAGCTTATATTCACTCGGTCAA
CAGCATGCCCATGCTGGAGGCGGAGCAGGAGTATCAGCTCGCCAAGCGTTTGCAGGAAACCGGTGACCTGCAGGCAGCAA
AGCAGCTGATTATGTCCCACCTGCGCTTTGTGGTTCATGTGGCCAAGAGCTACTCAGGCTATGGCCTGCCACAGGCCGAC
CTGATCCAGGAAGGCAACATAGGCCTGATGAAGGCCGTTAAACGTTTCGACCCTGATGTGGGTGTGCGTTTGGTGTCTTT
TGCCGTGCATTGGATCAAAGCAGAAATTCACGAATATGTGCTGAAAAACTGGCGCATTGTTAAAGTGGCCACCACCAAGG
CCCAGCGCAAGCTGTTCTTTAATCTGCGTAAAGCCAAACAGCGTCTGGGTTGGTTCTCGGATGACGAAGTGGCCATGGTG
GCAGAAAACCTGGGTGTGTCCAAAGACGATGTGACCGAGATGGAGTCGCGTATGGCGGCTCAGGATCAAGCCTTCGATTT
GGTCAACGATCACGACGACGATGAAGGCTTTGCTCCAGTGCACTATCTGGAAGACCACTCTTCCGATTTGGCCGCCAACA
TCGAAAACGACAATTGGGAATCCAATGCCCAGAATCGTTTGATTGCTGCCATTAAGACTCTCGATGAGCGCAGCCAGCAC
ATTCTGCGTGCCCGCTGGTTGGATGACGACAAGGTCACTCTCCAGGAGCTGGCGGAGCAGTATCAGGTGTCAGCTGAGCG
TATTCGTCAGCTTGAAAAGAACGCCATGAACAAGCTCAAGGCGCGTATGGAAGCCTGA

Upstream 100 bases:

>100_bases
GCTTGACATGACATGTCAGATGGCCGATAGTTCATCAGCCCGGTTTGGCCGCCATCCGGCGTCAGCTACGGGCAAATGTG
AGAAGTAATCAGGAGTACCA

Downstream 100 bases:

>100_bases
TAAGCTTCAATAAAAGAAAACCGGCCCAATGGCCGGTTTTTTTGTTGTCACTCTAAAACCACCTCCTATGGAGGTGGTGA
TCGTTCTAGTGGGGCTTTGC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTGQTQSMALMVPQGASSLEAYIHSVNSMPMLEAEQEYQLAKRLQETGDLQAAKQLIMSHLRFVVHVAKSYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKQRLGWFSDDEVAMV
AENLGVSKDDVTEMESRMAAQDQAFDLVNDHDDDEGFAPVHYLEDHSSDLAANIENDNWESNAQNRLIAAIKTLDERSQH
ILRARWLDDDKVTLQELAEQYQVSAERIRQLEKNAMNKLKARMEA

Sequences:

>Translated_285_residues
MTGQTQSMALMVPQGASSLEAYIHSVNSMPMLEAEQEYQLAKRLQETGDLQAAKQLIMSHLRFVVHVAKSYSGYGLPQAD
LIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKQRLGWFSDDEVAMV
AENLGVSKDDVTEMESRMAAQDQAFDLVNDHDDDEGFAPVHYLEDHSSDLAANIENDNWESNAQNRLIAAIKTLDERSQH
ILRARWLDDDKVTLQELAEQYQVSAERIRQLEKNAMNKLKARMEA
>Mature_284_residues
TGQTQSMALMVPQGASSLEAYIHSVNSMPMLEAEQEYQLAKRLQETGDLQAAKQLIMSHLRFVVHVAKSYSGYGLPQADL
IQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYVLKNWRIVKVATTKAQRKLFFNLRKAKQRLGWFSDDEVAMVA
ENLGVSKDDVTEMESRMAAQDQAFDLVNDHDDDEGFAPVHYLEDHSSDLAANIENDNWESNAQNRLIAAIKTLDERSQHI
LRARWLDDDKVTLQELAEQYQVSAERIRQLEKNAMNKLKARMEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=287, Percent_Identity=69.3379790940767, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1789098, Length=279, Percent_Identity=26.5232974910394, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=29.0598290598291, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32472; Mature: 32341

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGQTQSMALMVPQGASSLEAYIHSVNSMPMLEAEQEYQLAKRLQETGDLQAAKQLIMSH
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LRFVVHVAKSYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNLRKAKQRLGWFSDDEVAMVAENLGVSKDDVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
QDQAFDLVNDHDDDEGFAPVHYLEDHSSDLAANIENDNWESNAQNRLIAAIKTLDERSQH
HHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILRARWLDDDKVTLQELAEQYQVSAERIRQLEKNAMNKLKARMEA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TGQTQSMALMVPQGASSLEAYIHSVNSMPMLEAEQEYQLAKRLQETGDLQAAKQLIMSH
CCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
LRFVVHVAKSYSGYGLPQADLIQEGNIGLMKAVKRFDPDVGVRLVSFAVHWIKAEIHEYV
HHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LKNWRIVKVATTKAQRKLFFNLRKAKQRLGWFSDDEVAMVAENLGVSKDDVTEMESRMAA
HHCCEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHH
QDQAFDLVNDHDDDEGFAPVHYLEDHSSDLAANIENDNWESNAQNRLIAAIKTLDERSQH
HHHHHHHHCCCCCCCCCCCHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
ILRARWLDDDKVTLQELAEQYQVSAERIRQLEKNAMNKLKARMEA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]