Click here to switch to the map view.

The map label for this gene is aroK

Identifier: 119776500

GI number: 119776500

Start: 3994250

End: 3994765

Strand: Reverse

Name: aroK

Synonym: Sama_3368

Alternate gene names: 119776500

Gene position: 3994765-3994250 (Counterclockwise)

Preceding gene: 119776501

Following gene: 119776499

Centisome position: 92.77

GC content: 48.45

Gene sequence:

>516_bases
ATGGCTGAAAAACGAAATATTTTTCTGGTAGGCCCTATGGGCGCGGGCAAGAGCACCATAGGCCGTCATCTGGCTCAAAT
GCTGCACTTGGAATTCCATGACTCTGACCAAGAAATTGAAAGCCGTACTGGGGCTGACATTGCTTGGGTATTTGATGTTG
AAGGTGAAGAAGGTTTCCGTCGCCGCGAGGCGCAGGTGATAGCCGACTTAACCGAAAAGCAGGGTATTGTGCTGGCAACC
GGTGGTGGTTCAGTACAAAGCAAAGATATACGTAACTATCTGTCCGCAAGAGGGATTGTGGTATATCTCGAGACAACCAT
AGATAAGCAGGTTGCTCGTACCCAAAGAGACAAACGTCGTCCACTTTTGCAGGTTGATGATCCTCGTTCAGTGCTAGAGA
CACTGGCCGAGAGCCGTAATCCACTCTATGAAGAAATCGCTGATGTGATTGTCAAAACCGATGACCAAAGTGCCAAGGTC
GTTGCCAATCAAATCATCGAACAGCTGGGATTCTGA

Upstream 100 bases:

>100_bases
TAATCCCCGGTCAAGTCTCTTTAGAGCAAGGTACTGAGATCGCCATCCTGTTTGGAATGGCATAGGTAGACACTTCATAA
ACGATTCAGACGTATAAGAA

Downstream 100 bases:

>100_bases
CGCACCATGAAGCAAATTCAGGTTGATTTGGCAAATCGAAGTTATCCGATCCACATTGGCCCGAATTTGTTTGAAGATCA
GGAGCTCTTTGCGCCTGTGG

Product: shikimate kinase I

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAEKRNIFLVGPMGAGKSTIGRHLAQMLHLEFHDSDQEIESRTGADIAWVFDVEGEEGFRRREAQVIADLTEKQGIVLAT
GGGSVQSKDIRNYLSARGIVVYLETTIDKQVARTQRDKRRPLLQVDDPRSVLETLAESRNPLYEEIADVIVKTDDQSAKV
VANQIIEQLGF

Sequences:

>Translated_171_residues
MAEKRNIFLVGPMGAGKSTIGRHLAQMLHLEFHDSDQEIESRTGADIAWVFDVEGEEGFRRREAQVIADLTEKQGIVLAT
GGGSVQSKDIRNYLSARGIVVYLETTIDKQVARTQRDKRRPLLQVDDPRSVLETLAESRNPLYEEIADVIVKTDDQSAKV
VANQIIEQLGF
>Mature_170_residues
AEKRNIFLVGPMGAGKSTIGRHLAQMLHLEFHDSDQEIESRTGADIAWVFDVEGEEGFRRREAQVIADLTEKQGIVLATG
GGSVQSKDIRNYLSARGIVVYLETTIDKQVARTQRDKRRPLLQVDDPRSVLETLAESRNPLYEEIADVIVKTDDQSAKVV
ANQIIEQLGF

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=170, Percent_Identity=78.8235294117647, Blast_Score=275, Evalue=1e-75,
Organism=Escherichia coli, GI1786587, Length=159, Percent_Identity=32.7044025157233, Blast_Score=90, Evalue=8e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_SHEAM (A1SB14)

Other databases:

- EMBL:   CP000507
- RefSeq:   YP_929240.1
- ProteinModelPortal:   A1SB14
- SMR:   A1SB14
- STRING:   A1SB14
- GeneID:   4605615
- GenomeReviews:   CP000507_GR
- KEGG:   saz:Sama_3368
- NMPDR:   fig|326297.7.peg.3170
- eggNOG:   COG0703
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- PhylomeDB:   A1SB14
- ProtClustDB:   PRK05057
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19107; Mature: 18976

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 36-36 BINDING 60-60 BINDING 82-82 BINDING 120-120 BINDING 139-139 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKRNIFLVGPMGAGKSTIGRHLAQMLHLEFHDSDQEIESRTGADIAWVFDVEGEEGFR
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHHHH
RREAQVIADLTEKQGIVLATGGGSVQSKDIRNYLSARGIVVYLETTIDKQVARTQRDKRR
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCC
PLLQVDDPRSVLETLAESRNPLYEEIADVIVKTDDQSAKVVANQIIEQLGF
CCEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEKRNIFLVGPMGAGKSTIGRHLAQMLHLEFHDSDQEIESRTGADIAWVFDVEGEEGFR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCCCHHHH
RREAQVIADLTEKQGIVLATGGGSVQSKDIRNYLSARGIVVYLETTIDKQVARTQRDKRR
HHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHCC
PLLQVDDPRSVLETLAESRNPLYEEIADVIVKTDDQSAKVVANQIIEQLGF
CCEEECCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA