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The map label for this gene is dam [H]

Identifier: 119776497

GI number: 119776497

Start: 3990718

End: 3991560

Strand: Reverse

Name: dam [H]

Synonym: Sama_3365

Alternate gene names: 119776497

Gene position: 3991560-3990718 (Counterclockwise)

Preceding gene: 119776498

Following gene: 119776494

Centisome position: 92.69

GC content: 49.58

Gene sequence:

>843_bases
ATGACCATGAAACAAAGGGCCTTTCTAAAATGGGCCGGTGGCAAATTCAAGCTGATGGACGACCTGCGTCGCCATTTGCC
TGAAGGAAAACGCCTGGTTGAACCCTTTGTTGGCGCAGGTTCGGTTTTTCTCAACACCCAATACGATGCCTATCTACTTT
GCGATATCAATCAGGATTTGATTGGCCTCTATAAGATAGTTAAGGCGCAGCCCGAGGAGTATATTCGTGCAGCCAGGGCG
CTCTTTTGTGATGAGATGAATGCCAAAGACGCCTACTACGATATTCGTAGTCGCTTCAATAAATCCAAAGATCCCTTTAG
CCGCGCCGTCTATTTCCTGTACCTGAACCGTCATGGTTTCAATGGCTTGTGTCGTTACAACCGTAAGGGTGAATTTAACG
TTCCTTTCGGTTCTTACAAGAAGCCCTATTTTCCCGAGCAGGAAATCCGGGCCTTTTCTATCAAGGCGCAGCGAGCCGAG
TTTCGCTGTATAAGTTACGAGCAGGCGTTTGAGCTTGCGACCAGCGGCGATGTGATTTATTGCGACCCGCCCTATGCACC
CTTATCGACTACGGCAAGCTTTACCACCTATGTGGGGGCGGGCTTTACTTTAGACGATCAGGCGTTGTTGGCCCGTCACT
CACGTCACATTGCTGTCGAGCGTGGCATACCTGTGCTCATCAGTAATCACGATATCCCATTGACCCGCGGTTTATACCTG
GGCGCCAAACTGGAAACGGTAAAGGTGCAGCGCAATATCAGCCAAAAAGGCAGTGGGCGAAATAAAGTGGATGAACTGAT
GGCGCTCTATGATGAAACCTTTCATCTGGAAGCGCTGGATTGA

Upstream 100 bases:

>100_bases
CTCTTATCGACCGCTGGATTCGGTATCCGGCGGTGAAATTTCAAATTAAACAAAGTACAATCAGGGGCTTCTTTATCTCT
TCCGGCGGCGTTTCCCGATC

Downstream 100 bases:

>100_bases
TCTGCGGTTTGGCTGGAGCCGCTCTTTATTAACTCAATACCAGATTCACTACCCAGATAAGACCAAACACCATACAGGCT
GCCAATAACAAGCCCATCAG

Product: site-specific DNA-methyltransferase (adenine-specific)

Products: NA

Alternate protein names: DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase; M.VchADam [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MTMKQRAFLKWAGGKFKLMDDLRRHLPEGKRLVEPFVGAGSVFLNTQYDAYLLCDINQDLIGLYKIVKAQPEEYIRAARA
LFCDEMNAKDAYYDIRSRFNKSKDPFSRAVYFLYLNRHGFNGLCRYNRKGEFNVPFGSYKKPYFPEQEIRAFSIKAQRAE
FRCISYEQAFELATSGDVIYCDPPYAPLSTTASFTTYVGAGFTLDDQALLARHSRHIAVERGIPVLISNHDIPLTRGLYL
GAKLETVKVQRNISQKGSGRNKVDELMALYDETFHLEALD

Sequences:

>Translated_280_residues
MTMKQRAFLKWAGGKFKLMDDLRRHLPEGKRLVEPFVGAGSVFLNTQYDAYLLCDINQDLIGLYKIVKAQPEEYIRAARA
LFCDEMNAKDAYYDIRSRFNKSKDPFSRAVYFLYLNRHGFNGLCRYNRKGEFNVPFGSYKKPYFPEQEIRAFSIKAQRAE
FRCISYEQAFELATSGDVIYCDPPYAPLSTTASFTTYVGAGFTLDDQALLARHSRHIAVERGIPVLISNHDIPLTRGLYL
GAKLETVKVQRNISQKGSGRNKVDELMALYDETFHLEALD
>Mature_279_residues
TMKQRAFLKWAGGKFKLMDDLRRHLPEGKRLVEPFVGAGSVFLNTQYDAYLLCDINQDLIGLYKIVKAQPEEYIRAARAL
FCDEMNAKDAYYDIRSRFNKSKDPFSRAVYFLYLNRHGFNGLCRYNRKGEFNVPFGSYKKPYFPEQEIRAFSIKAQRAEF
RCISYEQAFELATSGDVIYCDPPYAPLSTTASFTTYVGAGFTLDDQALLARHSRHIAVERGIPVLISNHDIPLTRGLYLG
AKLETVKVQRNISQKGSGRNKVDELMALYDETFHLEALD

Specific function: Methylates DNA within the sequence GATC. Directly involved in methyl-directed DNA mismatch repair [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=267, Percent_Identity=62.9213483146067, Blast_Score=340, Evalue=4e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 32110; Mature: 31979

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKQRAFLKWAGGKFKLMDDLRRHLPEGKRLVEPFVGAGSVFLNTQYDAYLLCDINQDL
CCCCCCCCEEECCCEEEHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCEEEEEECCHHH
IGLYKIVKAQPEEYIRAARALFCDEMNAKDAYYDIRSRFNKSKDPFSRAVYFLYLNRHGF
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHEEEEEEEECCCC
NGLCRYNRKGEFNVPFGSYKKPYFPEQEIRAFSIKAQRAEFRCISYEQAFELATSGDVIY
CCEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCEEEECHHHHHHHCCCCCEEE
CDPPYAPLSTTASFTTYVGAGFTLDDQALLARHSRHIAVERGIPVLISNHDIPLTRGLYL
ECCCCCCCCCCCCHHHHCCCCCEECHHHHHHHCCCCEEEECCCEEEEECCCCCEECCEEE
GAKLETVKVQRNISQKGSGRNKVDELMALYDETFHLEALD
CCEEEEEEEECCHHHCCCCCHHHHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
TMKQRAFLKWAGGKFKLMDDLRRHLPEGKRLVEPFVGAGSVFLNTQYDAYLLCDINQDL
CCCCCCCEEECCCEEEHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCEEEEEECCHHH
IGLYKIVKAQPEEYIRAARALFCDEMNAKDAYYDIRSRFNKSKDPFSRAVYFLYLNRHGF
HHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHEEEEEEEECCCC
NGLCRYNRKGEFNVPFGSYKKPYFPEQEIRAFSIKAQRAEFRCISYEQAFELATSGDVIY
CCEEEECCCCCCCCCCCCCCCCCCCHHHHEEEEEEECCCCEEEECHHHHHHHCCCCCEEE
CDPPYAPLSTTASFTTYVGAGFTLDDQALLARHSRHIAVERGIPVLISNHDIPLTRGLYL
ECCCCCCCCCCCCHHHHCCCCCEECHHHHHHHCCCCEEEECCCEEEEECCCCCEECCEEE
GAKLETVKVQRNISQKGSGRNKVDELMALYDETFHLEALD
CCEEEEEEEECCHHHCCCCCHHHHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11705940 [H]