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The map label for this gene is dsbA [H]

Identifier: 119776453

GI number: 119776453

Start: 3949999

End: 3950613

Strand: Reverse

Name: dsbA [H]

Synonym: Sama_3321

Alternate gene names: 119776453

Gene position: 3950613-3949999 (Counterclockwise)

Preceding gene: 119776454

Following gene: 119776452

Centisome position: 91.74

GC content: 51.06

Gene sequence:

>615_bases
ATGAAAAAAGCACTCTTGATGGCAGCTGCGCTGCTGGTTGCTCCCCTGATGTCCATGGCCGCCGATTTCAAAGAAGGTGT
GCATTACGAAGTCATTAACGAAGGTCCTGGCAGTGCCAAGCCCGAAATCACCGAGTTTTTCTCTTTCTACTGCCCACACT
GCTACAACTTCTCCAAAACAGTTGTTCCCATGATCGAAGCCAAAAAGCCTGAAGGTGTTGCCTTCAATCAGGCTCACGTT
GACTTTATCGGCCGTGAAATGGGCCCGGAAATGTCACGCGCCTTCGCCGTGGCGCATCAGCTGAAAGTCGAAAAGAAAAT
GGAAAAGGCCCTGTTTGCCGCTATCCACGACAAAAAGCAGCACTTCACCACCCGTGACGACGTGCGTGCCCTGTTTGTGG
CCAATGGTGTGGATGGCAAAGACTTCGATTCTGCTGCCAACTCCTTTATGGTGAACGCCCAGATGTCGAAGATGAAGCGT
GATACCGAAAACGCCAAGCTGTCCGGTGTGCCTTCCCTGGTGGTTAACGGCAAGTACAAGGTACTGACCGATTCCATCAA
GAGCTACGAAGAAATGCTGGATATCGCTTTCTACCTGGCTACCAAAAAGGACTGA

Upstream 100 bases:

>100_bases
AAAGTTGAACTAGGGGCTTGAGATGGCATCAAAAAGCGTATTAGTTTAGGCATCGCCCAATGAGATTTGGTCCACCGGAC
CCGCTAAGCAAGGAAATAAC

Downstream 100 bases:

>100_bases
GTTTAAACACTCAACGAAAAAAGCGCCCCAGAGGCGCTTTTTTTATCCCCCAAAGGGGCTCAGAGAGGGGGCTCAGGCCG
TAAGTCGATGAAAACCCTGA

Product: thiol:disulfide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MKKALLMAAALLVAPLMSMAADFKEGVHYEVINEGPGSAKPEITEFFSFYCPHCYNFSKTVVPMIEAKKPEGVAFNQAHV
DFIGREMGPEMSRAFAVAHQLKVEKKMEKALFAAIHDKKQHFTTRDDVRALFVANGVDGKDFDSAANSFMVNAQMSKMKR
DTENAKLSGVPSLVVNGKYKVLTDSIKSYEEMLDIAFYLATKKD

Sequences:

>Translated_204_residues
MKKALLMAAALLVAPLMSMAADFKEGVHYEVINEGPGSAKPEITEFFSFYCPHCYNFSKTVVPMIEAKKPEGVAFNQAHV
DFIGREMGPEMSRAFAVAHQLKVEKKMEKALFAAIHDKKQHFTTRDDVRALFVANGVDGKDFDSAANSFMVNAQMSKMKR
DTENAKLSGVPSLVVNGKYKVLTDSIKSYEEMLDIAFYLATKKD
>Mature_204_residues
MKKALLMAAALLVAPLMSMAADFKEGVHYEVINEGPGSAKPEITEFFSFYCPHCYNFSKTVVPMIEAKKPEGVAFNQAHV
DFIGREMGPEMSRAFAVAHQLKVEKKMEKALFAAIHDKKQHFTTRDDVRALFVANGVDGKDFDSAANSFMVNAQMSKMKR
DTENAKLSGVPSLVVNGKYKVLTDSIKSYEEMLDIAFYLATKKD

Specific function: Involved in disulfide-bond formation. Required for the functional maturation of secreted virulence factors. Acts by transferring its disulfide bond to other proteins [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=211, Percent_Identity=34.1232227488152, Blast_Score=137, Evalue=7e-34,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22702; Mature: 22702

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKALLMAAALLVAPLMSMAADFKEGVHYEVINEGPGSAKPEITEFFSFYCPHCYNFSKT
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCH
VVPMIEAKKPEGVAFNQAHVDFIGREMGPEMSRAFAVAHQLKVEKKMEKALFAAIHDKKQ
HHHHHCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFTTRDDVRALFVANGVDGKDFDSAANSFMVNAQMSKMKRDTENAKLSGVPSLVVNGKYK
HCCCHHHHEEEEEECCCCCCCHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCEEEECCCHH
VLTDSIKSYEEMLDIAFYLATKKD
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKALLMAAALLVAPLMSMAADFKEGVHYEVINEGPGSAKPEITEFFSFYCPHCYNFSKT
CCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCH
VVPMIEAKKPEGVAFNQAHVDFIGREMGPEMSRAFAVAHQLKVEKKMEKALFAAIHDKKQ
HHHHHCCCCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HFTTRDDVRALFVANGVDGKDFDSAANSFMVNAQMSKMKRDTENAKLSGVPSLVVNGKYK
HCCCHHHHEEEEEECCCCCCCHHHHHHHEEEHHHHHHHHHHCCCCCCCCCCEEEECCCHH
VLTDSIKSYEEMLDIAFYLATKKD
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1631111; 1324389; 10952301; 9149147 [H]